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    Lab2 (Raw Assay)

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    Lana Raed
    This document describes an experiment to determine the purity of paracetamol raw material using UV spectroscopy. A calibration curve is constructed using standard paracetamol solutions to determine the absorptivity value. Then, the absorbance of a diluted raw paracetamol solution is measured and Beer's law is applied to calculate the concentration. The actual concentration is then used to determine the actual weight and purity of the raw material sample.

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    Lab2 (Raw Assay)

    Uploaded by

    Lana Raed
    133 views18 pages
    This document describes an experiment to determine the purity of paracetamol raw material using UV spectroscopy. A calibration curve is constructed using standard paracetamol solutions to determine the absorptivity value. Then, the absorbance of a diluted raw paracetamol solution is measured and Beer's law is applied to calculate the concentration. The actual concentration is then used to determine the actual weight and purity of the raw material sample.

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    This document describes an experiment to determine the purity of paracetamol raw material using UV spectroscopy. A calibration curve is constructed using standard paracetamol solutions to determine the absorptivity value. Then, the absorbance of a diluted raw paracetamol solution is measured and Beer's law is applied to calculate the concentration. The actual concentration is then used to determine the actual weight and purity of the raw material sample.

    Copyright:

    © All Rights Reserved
    Download as PPSX, PDF, TXT or read online from Scribd
    Download as ppsx, pdf, or txt
    133 views18 pages

    Lab2 (Raw Assay)

    Uploaded by

    Lana Raed
    This document describes an experiment to determine the purity of paracetamol raw material using UV spectroscopy. A calibration curve is constructed using standard paracetamol solutions to determine the absorptivity value. Then, the absorbance of a diluted raw paracetamol solution is measured and Beer's law is applied to calculate the concentration. The actual concentration is then used to determine the actual weight and purity of the raw material sample.

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    You are on page 1of 18

    University of Jordan

    faculty of pharmacy

    Pharmaceutical instrumental analysis laboratory


    Experiment 2
    Assay of Paracetamol raw material
    Aim of the experiment
    • To be able of determining the purity of a pharmaceutical
    compound using UV spectroscopy.
    INTRODUCTION AND
    THEORY
    Theoretical background
    • Paracetamol is an Analgesic and
    antipyretic compound.
    • Molecular formula: C8H9NO2 and
    Mwt =151.2g/mol
    • Characters: White, crystalline
    powder. Sparingly soluble in water,
    freely soluble in alcohol, very
    slightly soluble in methylene
    chloride.
    Theoretical background
    • Percentage purity of a pharmaceutical compound =
    • [Practically found weight / theoretical weight ] x 100%
    Beer’s- Lambert’s law
    • A = A1%. b. C
    • A : absorbance,
    • B: path length (mostly 1cm)
    • C : concentration expressed as g% and
    • A1% : a physical constant known as absorptivity of the compound at
    a particular wavelength.
    Beer’s- Lambert’s law
    • The value of A1% is usually given by the pharmacopeia in
    pharmacopoeial procedure or else it can be determined
    practically in the lab.
    • In order to be able to obtain the value of A1%, a reference standard
    material of the analyte should be available; if so prepare a series
    of standard solution of the material and measure them properly
    at the desired wavelength.
    • Plot the obtained values of absorbance against corresponding
    concentrations. The slope of the obtained linear line should
    represent A1%
    PRACTICAL PART
    Procedure

    ●
    To determine the absorptivity of paracetamol

    A. Calibration curve
    Be careful!?
    • We will be using two paracetamol powder
    • Paracetamol standard powder ,which is 100% pure
    paracetamol, for the absorptivity measurement
    (calibration curve)
    • Paracetamol raw powder which purity is to be
    determined.
    Procedure
    • 1. Calibration curve for paracetamol
    •3. From stock 2 pipette the following
    volumes in 50 ml volumetric flasks and
    complete the volume with 0.1M NaOH

    2ml
    • 1. Dissolve 0.085g •2. From stock 1 pipette 4. Scan the
    paracetamol standard in 2 ml and complete the most
    50 ml flask using 0.1M volume up to 50ml with 4ml concentrated
    NaOH 0.1M NaOH solution to
    6ml determine
    lambda max of
    8ml paracetamol
    then measure
    the absorbance
    of the 5
    Stock 1 Stock 2 10ml
    solutions
    Procedure
    • Calibration curve results
    • The link below will help you in plotting calibration curve via excel
    •
    https://www.youtube.com/watch?v=8efZRBCFpRg

    Solution concentration Absorbance

    0.00027 0.195
    0.00054 0.394
    0.000816 0.58
    0.00109 0.75
    0.00136 0.902
    Procedure
    • 2. Raw assay

    • 1. Dissolve 0.085g 2. From stock 1 pipette


    3. From stock 2 pipette 4. Measure the
    paracetamol raw material 2ml into 50 ml v. flask
    5ml into 50 ml v. flask absorbance of the
    in 50 ml flask using 0.1M and complete the
    and complete the resulting solution
    NaOH volume with 0.1M
    volume with 0.1M at 257nm
    NaOH
    NaOH

    Stock 1 Stock 2 Diluted raw solution


    Purity calculation

    • 1. For the results of the experiments we can apply Beers’


    law to calculate the concentration of the diluted raw
    solution .

    •A=abc
    Absorbance Concentration
    absorptivity (1 cm)
    (raw (unknown) ?
    (calibration
    material )
    curve )
    Purity calculation

    • 2. note that the concentration we get from beers law is


    for diluted raw solution, so it should be multiplied by the
    dilution factor to get the original concentration.
    • Concentration before dilution= Concentration after* dilution factor
    Purity calculation

    • 3. Now we need to convert the concentration to mass by


    multiplying by the volume of the solution.
    • Mass(g) = concentration(g/100ml)* volume (ml)
    Purity calculation

    • 4. purity is ready to be determined


    • Purity = (Actual wt/ theoretical wt )*100%

    The mass you


    got From the The mass you
    calculation weight on the
    balance

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