Dat

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Cl
ass10

Topic:Or thomy xov i

r uses.Labor atorydi agnosisofi nfluenzai nfect i
on.
Paramy xov iruses.Labor at or ydi agnosi sofpar ainf l
uenza, measl es, andmumps.
ISW:Adenov i
ruses.Labor ator ydi agnosi sofadenov i
ral i
nfecti
ons.
Quest i
onst obedi scussed:
1.Char acteristi
cf eat uresoft heFami lyOr t
homy xov ir
idae.
2.Mor phol ogyandst r
uct ur eoft heinfluenzav irus.
3.Ant igeni cst r
uct ur eoft hev irusesf rom GenusI nf luenzav i
rus.
4.Ant igeni cshi ftandant igeni cdr if
toft ypeAi nfluenzav iruses.
5.Cul turingofi nf l
uenzav i
ruses.
6.Pat hogenesi sandcl inical sympt omsofi nfluenza.
7.Immuni tyini nfl
uenzav i
rusi nf ecti
ons.
8.Labor atorydi agnosi sofi nf l
uenza.
9.Ant i-i
nf l
uenzachemot her apeut i
cdr ugsandi mmunobi ological preparations.
10.Classificationandchar act erist i
cfeat uresoft heFami l
yPar amy xovir
idae
11.Mor phol ogyandst r
uct ur eoft hepar amy xov irusv ir
ion.
12.Repr oduct ivecy cl eandcul turingofpar amy xov iruses.
13.GenusRespi rovirus.Par ainf l
uenzav i
rus:Pat hogenesi s,cl
inicalsy mpt omsst r
ucture
andlabor ator ydiagnosi s.
14.GenusRubul avirus.Pat hogenesi sandcl i
nical sy mptomsofmumps.
15.GenusMor bill
ivi
rus.Char act er i
sti
cf eaturesoft hemeasl esv ir
usandv i
ruscausing
SSPE.Pat hogenesi sandcl inical sympt omsofmeasl es.
16.Speci fi
cpr ophy l
ax isofmumpsandmeasl es.
Pr acti
calt asks:
1.Label mai nst ruct ural element softhei nfluenzav iri
on.

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2.Listthemai n st
agesoftheinfl
uenzavir
usrepr
oducti
vecy cl
eand it
s unique
featur
es.Expl
ainamechani
sm ofr
emantadi
neandosel
tamiv
iranti
vi
ral
acti
vi
ty.
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3.Li
stmethodsusedforinfl
uenzavir
usculturi
ngandindi
cationofthevir
usincult
ure:
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4.Runhemaggl uti
nat
iont
estwithal
lantoi
cfl
uidoft hechickenembry
oinf
ect
edwith
nasalwashingsofthei
nfl
uenzapati
entandincubated at37°Сfor72h.Readand
drawtheresult
.

1:
4 1:
8 1:
16 1:
32 1:
64 Cont
rol
-
NaCl0,
85%

Concl
usi
on:___
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5.Readandwri
tedowntheresul
toft hehemagglutinati
oni
nhibi
tiontest(
HItest)
,
perf
ormedwit
hthefort
hepatientsvir
usident
ifi
cationwit
h3t ypesofsera.
A)ant
i-
A/Cal
i
for
nia/7/
2009;B)anti
-A/Cali
for
nia/4/2009;C)anti
-A/Bri
sbane/59/
2007

6.Compl
eteaschemeofl
abor
ator
ydi
agnosi
sofi
nfl
uenza:
Speci
men:

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hod _
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_met
hod

IFt
est 1.Vi
rusi
sol
ati
on:
(
toi
dent
if
y__
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_) Deter
minati
onoft heAb
Chi
ckenembr
yo ti
ter
sinpair
edser a:
Cel
lcul
tur
e HIt est
PCR CFT
(
toi
dent
if
y___
___
__) ELISA

I
mmunochromatogr
aphy 2.Vi
rusdet
ect
ion:
(t
oident
if
y__
_____
__)
HAtest HAdst
est

3.Vir
usident
if
icat
ion:
HItest HadsIt
est
CFT
ELI
SA

7.Descri
beact ualt
ypeofant i
-i
nfl
uenzavacci
ne(quadri
val
entfl
u:A(H1N1)
,A(
H2N3),B
(y
amagat a),B(Vi
ctori
a)andgeneralschemesofit
smanuf act
uri
ng:
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8.Label
mai
nst
ruct
ural
element
soft
hemeasl
esv
iri
on.

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9.Compl
eteaschemeoft
hepar
amy
xov
irusr
epr
oduct
ivecy
cleandl
i
sti
tsmai
nst
ages.
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10.Li
stmet hodsusedf orparainfl
uenzaviruscult
uri
ngandi ndicati
onoft
hev
irusi
n
cul
ture::___
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11.Compl eteaschemeofal aborator
ydiagnosisofparai
nfluenza.

Speci
men:

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hod _
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hod

IFtest 1.I
sol
ationofvir
us: Demonst
rati
onofAbi
n
(
Ident
if
icat
ionof ___
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_____ pai
redbl
oodseraby:
…………) ___
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__ CFT
I
Hads
PCR ELISA
(I
dent
if
icat
ion 2.Vir
usdetecti
on:__
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of…………) ___
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3.Vir
usident
ifi
cat
ion:
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12.
Descr
ibepr
epar
ati
onsusedf
ort
hepr
ophy
laxi
sofpar
amy
xov
irusesi
nfect
ions:

MMR:__
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ISW:Adenov i
ruses.Laborator
ydiagnosisofadenovir
alinf
ecti
ons.
1.Generalcharacter
isti
candclassif
icati
onofadenovir
uses.
2.Str
uctureandr epl
icati
onofadenov i
ruses.
3.Pathogenesisofadenov i
rali
nfecti
ons.
4.Laborat
orydiagnosisofadenov i
ralinf
ecti
ons.
Teacher’
ssignat
ure_
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