Scribd Logo
Upload

Welcome to Scribd!

  • 12 views

    Rheumatoid Factors

    Uploaded by

    Evelyn Medina

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Rheumatoid Factors

    Uploaded by

    Evelyn Medina
    12 views10 pages

    Original Title

    Rheumatoid_factors.pptx

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    12 views10 pages

    Rheumatoid Factors

    Uploaded by

    Evelyn Medina

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 10

    Rheumatoid factors

    RF
    • Rheumatoid factors (RF) are antibodies
    directed against antigenic sites in the Fc
    fragment of human and animal IgG.
    • Their frequent occurrence in rheumatoid
    arthritis makes them useful for diagnosis and
    monitoring of the disease
    TEST PRINCIPLE
    • The RF reagent is a suspension of polystyrene latex particles
    sensitized with specially prepared human IgG.
    • The reagent is based on an immunological reaction between
    human IgG bound to biologically inert latex particles and
    rheumatoid factors in the test specimen.
    • When serum containing rheumatoid factors is mixed with
    the latex reagent, visible agglutination occurs.
    • The RF latex reagent sensitivity has been adjusted to detect
    a minimum of 8 IU/mL of rheumatoid factors according with
    the WHO International Standard without previous sample
    dilution.
    SPECIMEN COLLECTION AND PREPARATION

    1. Use fresh serum collected by centrifuging clotted


    blood.
    2. If the test cannot be carried out on the same
    day, the serum may be stored between 2 - 8°C
    for no longer than 72 hours after collection.
    3. For longer periods the sample must be frozen.
    4. As in all serological tests, hemolytic or
    contaminated serum must not be used.
    5. Do not use plasma.
    Materials provided with the test kits
    • 1. RF Latex Reagent: A suspension of uniform polystyrene
    particles coated with IgG (human) in glycine buffer, pH 8.2.
    MIX WELL BEFORE USING.
    • 2. RF Positive Control Serum: A stabilized, prediluted human
    serum containing at least 8 IU/mL of RF.
    • 3. RF Negative Control Serum: A stabilized, prediluted human
    serum containing less than 8 IU/mL of RF.
    • 4. Glycine-Saline Buffer (20x): pH 8.2 ± 0.1M glycine and
    0.15M NaCl
    • 5. Reaction Slide.
    • 6. Pipette/Stir Sticks
    Qualitative Test

    • 1. Bring reagents and specimens to room temperature before use. 2. Place


    one drop (50 µl) of the RF Positive Control on field #l of the reaction slide.
    Place one drop (50 µl) of the RF Negative Control on field #2. The remaining
    fields are used for test specimens. Using pipettes provided. Place one drop
    of the undiluted specimens on successive fields. Retain Pipette/Stir Sticks for
    mixing step.
    • 3. Gently resuspend the RF Latex Reagent and add one drop to each test
    field. Use pipette/Stir Stick to spread reaction mixture over entire test field.
    • 4. Rotate the slide manually or with a mechanical rotor at 80-100 rpm for 2
    minutes and read immediately under direct light.
    • 5. Presence of agglutination of the latex particle is a positive result (see
    figure 1). Agglutination indicates a RF concentration of equal or more than 8
    IU/ml. Sera with positive agglutination should be run again with the
    Quantitative Test.
    Quantitative Test

    • 1. Bring reagents and specimens to room temperature before use.


    • 2. Using the Glycine-Saline Buffer, dilute the specimens 1:2, 1:4, 1:8, 1:16,
    1:32 or as needed.
    • 3. Place one drop (50 µl) each of negative and positive controls on two
    slide rings. Place on drop (50 µl) of each dilution on successive fields of
    the reaction slide.
    • 4. Gently resuspend the RF Latex Reagent and add one drop to the
    reaction slide.
    • 5. Gently resuspend the RF Latex Reagent and add one drop to each test
    field. Use Pipette/Stir Stick to spread reaction mixture over entire field.
    • 6. Rotate the slide for 2 minutes and read immediately under direct light
    https://www.youtube.com/watch? •
    v=X8vwC26f74M

    You might also like