Scribd Logo
Upload

Welcome to Scribd!

  • 199 views

    Biochemistry Assignment-Week 1: NPTEL Online Certification Courses Indian Institute of Technology Kharagpur

    Uploaded by

    Samarjeet Kumar Singh
    This document contains a 15 question multiple choice quiz on biochemistry topics. The questions cover topics like: [1] how antibiotics affect bacterial growth, [2] buffer pH relationships, [3] amino acid properties such as glycine's lack of optical activity, [4] Anfinsen's experiment conclusions on protein folding, and [5] protein structural elements such as alpha helices and beta sheets. For each question, the correct multiple choice answer is provided along with a short explanation of the reasoning.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Biochemistry Assignment-Week 1: NPTEL Online Certification Courses Indian Institute of Technology Kharagpur

    Uploaded by

    Samarjeet Kumar Singh
    199 views8 pages
    This document contains a 15 question multiple choice quiz on biochemistry topics. The questions cover topics like: [1] how antibiotics affect bacterial growth, [2] buffer pH relationships, [3] amino acid properties such as glycine's lack of optical activity, [4] Anfinsen's experiment conclusions on protein folding, and [5] protein structural elements such as alpha helices and beta sheets. For each question, the correct multiple choice answer is provided along with a short explanation of the reasoning.

    Original Title

    BIOCHEMISTRY W1

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document contains a 15 question multiple choice quiz on biochemistry topics. The questions cover topics like: [1] how antibiotics affect bacterial growth, [2] buffer pH relationships, [3] amino acid properties such as glycine's lack of optical activity, [4] Anfinsen's experiment conclusions on protein folding, and [5] protein structural elements such as alpha helices and beta sheets. For each question, the correct multiple choice answer is provided along with a short explanation of the reasoning.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    199 views8 pages

    Biochemistry Assignment-Week 1: NPTEL Online Certification Courses Indian Institute of Technology Kharagpur

    Uploaded by

    Samarjeet Kumar Singh
    This document contains a 15 question multiple choice quiz on biochemistry topics. The questions cover topics like: [1] how antibiotics affect bacterial growth, [2] buffer pH relationships, [3] amino acid properties such as glycine's lack of optical activity, [4] Anfinsen's experiment conclusions on protein folding, and [5] protein structural elements such as alpha helices and beta sheets. For each question, the correct multiple choice answer is provided along with a short explanation of the reasoning.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 8

    NPTEL Online Certification Courses

    Indian Institute of Technology Kharagpur

    Biochemistry
    Assignment- Week 1
    TYPE OF QUESTION: MCQ
    Number ofquestions:15 Total mark: 15 X 1 = 15

    QUESTION 1:

    Many antibiotics interfere with the transfer of genetic information from RNA to protein, preventing
    bacteria from growing. Which of the following processes is affected by antibiotics?

    a) Transmission
    b) Translation
    c) Transcription
    d) Replication

    Correct Answer: b
    Detail Answer:
    During translation, RNA is used as a code to create proteins. If antibiotics interfere with the ability
    for RNA to transfer information to proteins, then translation is being affected.

    QUESTION 2:

    Three buffers are made by combining a 1M solution of acetic acid with a 1M solution of sodium
    acetate in the ratios shown below.
    1M acetic acid 1M sodium acetate
    Buffer 1 10ml 90ml
    Buffer 2 50ml 50ml
    Buffer 3 90ml 10ml

    Which of these statements is true of the resulting buffers?

    a) pH of Buffer 1< pH of Buffer 2< pH of Buffer 3


    b) pH of Buffer 1 = pH of Buffer 2=pH of Buffer 3
    c) pH of Buffer 1 > pH of Buffer 2 > pH of Buffer 3
    d) The problem cannot be solved without knowing the value of pKa

    Correct Answer: c

    Detail Answer:
    NPTEL Online Certification Courses
    Indian Institute of Technology Kharagpur

    QUESTION 3:

    Of the 20 standard amino acids, only ___________ is not optically active. The reason is that its
    side chain ___________.
    a) alanine; has a simple methyl group
    b) glycine; has only a hydrogen atom
    c) glycine; is branched
    d) proline; forms a covalent bond with the amino group

    Correct Answer: b

    Detail Answer:

    Optical activity depends on the asymmetry of the central C atom. In glycine, the central C atom has
    two H atom attached to it, whereas in all other 19 amino acid, the central C is asymmetric with 4
    different groups attached to it.

    QUESTION 4:

    The significant conclusion from Anfinsen's work on Ribonuclease A was that


    a) Disulfide bonds (S-S) in proteins can be reduced in vitro.
    b) The -SH groups of cysteine are not found in vivo.
    c) The native conformation of a protein is adopted spontaneously.
    d) Irreversible denaturation of proteins violates the "Thermodynamic Hypothesis".

    Correct Answer: c

    Detail Answer: Sequence determines structure – this is the conclusion from the experiment.

    QUESTION 5:

    Proteins can be stabilized by ionic bonds that could include the interaction of the side chain of a
    Lysine residue with the side chain of ________.
    a) Gly
    b) Arg
    c) His
    d) Asp

    Correct Answer: d
    NPTEL Online Certification Courses
    Indian Institute of Technology Kharagpur

    QUESTION6:

    The unique cyclic structure of which of the following amino acids plays a central role in the
    formation of alpha helices and beta sheets?

    a) Proline
    b) Arginine
    c) Valine
    d) Tyrosine

    Correct Answer: a
    Detail Answer:

    QUESTION 7:

    The most likely amino acid that would yield the above titration curve would be
    a) Arginine
    b) Glutamic acid
    c) Glycine
    d) Histidine

    Correct Answer: c

    Detail Answer: There is only one inflection point indicating that the amino acid is not charged.
    NPTEL Online Certification Courses

    Indian Institute of Technology Kharagpur

    QUESTION 8:
    Which is a difference between an α-helix and a β-pleated sheet?
    a) An α-helix is a right-handed structure containing hydrogen bonds while a β-pleated sheet is a
    left-handed structure containing only ionic bonds.
    b) An α-helix has a relatively extended spiral shape while a β-pleated sheet has a relatively
    compact zig-zag shape.
    c) An α-helix has non-covalent bonds between amino acids near each other in the sequence while
    a β-pleated sheet has non-covalent bonds between amino acids far apart in the sequence.
    d) An α-helix contains only amino acids with polar R-groups while a β-pleated sheet contains
    only amino acids with non-polar R-groups.

    Correct Answer: c
    Detail Answer:

    QUESTION 9:

    The repeating structural unit in a multimeric protein is known as a(n):

    a) Domain.
    b) Motif.
    c) Oligomer.
    d) Protomer.

    Correct Answer: d
    Detail Answer:
    In structural biology, a protomer is the structural unit of an oligomeric protein. It is the smallest
    unit composed of at least two different protein chains that form a larger hetero-oligomer by
    association of two or more copies of this unit.
    NPTEL Online Certification Courses
    Indian Institute of Technology Kharagpur

    QUESTION 10:

    The central dogma states that DNA determines an organism’s traits, such as eye color or hair color.
    Which sequence best represents the relationship between DNA and the traits of an organism?

    Correct Answer: a
    Detail Answer:
    A DNA base sequence in an organism is transcribed to mRNA, which are then translated into
    amino acid sequences. These sequences determine protein shape, and the protein shape determines
    protein function. The function of proteins affects the expression of a particular trait.

    QUESTION 11:

    In the diagram below, the plane drawn at the peptide bond indicates the:

    a) Plane of rotation around the Cα– N bond


    b) Absence of rotation around the C-N bond
    because of its partial double bond character.
    c) Region of steric hindrance determined by the
    large C=O group
    d) Theoretical space between -180o and +180o that
    can be occupied by the φ and ψ angles in the peptide
    bond

    Correct Answer: b

    Detail Answer:
    NPTEL Online Certification Courses
    Indian Institute of Technology Kharagpur

    QUESTION 12:

    What effect would converting a glutamic acid to γ-carboxyglutamate have on the overall charge of
    the protein at pH 7.0?

    Structure of γ-carboxyglutamate

    a) It will become more negative.


    b) It will become more positive.
    c) It will stay the same.
    d) There is not enough information to answer the question.

    Correct Answer: a
    Detail Answer:
    γ-carboxyglutamate has 3 COOH group. pKa of glutamic acid is less than 7, so at pH=7.0 the
    solution will act as base and COOH group will be converted to COO-

    QUESTION 13:

    Which of the following statements about cysteine is correct?

    a) Two cystines are released when a —CH2—S—S—CH2— disulfide bridge is oxidized.


    b) Cystine forms when the —CH2—SH group is oxidized to form a —CH2—S—S—CH2—
    disulfide bridge between two cysteines.
    c) Cysteine is an example of a nonstandard amino acid, derived by linking two standard amino
    acids.
    d) Cystine is formed by the oxidation of the carboxylic acid group of cysteine.

    Correct Answer: b
    Detail Answer:
    NPTEL Online Certification Courses
    Indian Institute of Technology Kharagpur

    QUESTION 14:

    You are given a mixture that contains Glutamic acid (pI = 3.2), Arginine (pI = 10.8), and Valine
    (pI= 6.0), and you subject the mixture to electrophoresis. Which amino acids migrate towards the
    cathode when the electrophoresis is carried out at a pH of 7.1?

    a) Glutamic acid
    b) Arginine
    c) Valine
    d) Both glutamic acid and valine

    Correct Answer: b
    Detail Answer:
    As the electrophoresis is carried out at a pH 7.1, Glutamic acid and Valine, having pH less than 7.1
    will remain negatively charged, whereas Arginine at this pH will be positively charged
    (Isoelectronic).

    QUESTION 15:

    The folded states of globular proteins in aqueous solutions are primarily stabilized by ------
    a) Hydrophobic interactions
    b) Peptide bonds
    c) Phosphodiester bonds
    d) Ionic bonds

    Correct Answer: a
    Detail Answer:

    ************END***********

    You might also like