Birla Institute of Technology and Science, Pilani Pilani Campus Instruction Division

BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
Instruction Division
First semester 2021-2022 Date: 25-09-2021
COURSE HANDOUT (PART-II)

In addition to part-I (General Handout for all courses appended to the timetable) this portion gives further specific
details regarding the course.
Course No. : BIO F110
Course Title : BIOLOGY LABORATORY
Instructor-in-charge : SANDHYA AMOL MARATHE
Team of Instructors : Dr. Meghana Tare, Dr. B. Vani, Dr. Balakumaran Chandrasekar, Sonakshi Puri, Saurabh
Singh, Sayantani Chatterjee, Sumukh Thakar, Ankita Sharma, Aishwarya Singh, Propanna Bandyopadhyay,
Gunjan, Harshita Sharma, Hansa Sehgal, Priyanka Roy.
Course Description:
Understanding Biological sciences, analysis and estimation of biomolecules, study of cell structure and division,
investigation of catalytic activity of enzyme, physiology of plant and animal systems, diversity of living systems.
Scope and Objective of the course:

The major objective of this course is to offer an experience on fundamental aspects of practical biology. The
student would observe and understand various biological phenomena and also be equipped with some simple
techniques which form the basis of research in biology. The course has been revised to cover some key concepts
taught in General Biology classes.
Text Book/Manual: Laboratory Manual for Biology, BITS Pilani 2014, revised, 2019.
Reference Book: Simon, E.J. et al: Campbell Essential Biology with Physiology (5th Edition, BITS Pilani custom
edition). Noida: Perason India Education Services Pvt. Ltd., 2015
Experiment Plan
Experiment No. Name of the Experiment Learning Outcome
Experiment – 1: Scientific Process Lab Understanding Scientific process, and its

components, learning to design experiments using
different variables while devising a testable
hypotheses, learning to present the results.
Experiment-2: Protein Lab: Measurement of total protein content Proteins: the building blocks; their role in humans;
in the given sample by Lowry’s method. methods to quantify proteins in different samples.
Experiment – 3: Enzyme Lab: To study the effect of the enzyme Properties of an enzyme and chromogenic
lactase on milk detection methods.
Experiment – 4: DNA Lab: To extract total genomic DNA from Basic knowledge about the genetic material,
cells principle of its isolation.
Experiment – 5: Electrophoresis Lab: Visualizing DNA using gel Understand characterization of DNA based on
electrophoresis molecular weight, using electrophoresis.
Please Do Not Print Unless Necessary

BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
Instruction Division
Experiment – 6: Transport Lab: To study the phenomenon of Effect of tonicity of a solution on different cell
plasmolysis in onion peel. types; osmosis & osmoregulation.
Experiment – 7: Transpiration Lab: Preparation of temporary mount Understanding the role of stomata in controlling
of leaf epidermis to study the structure of stomata transpiration in plants. Transpiration rate and
and measurement of transpiration rate factors affecting it.
Experiment – 8: Photosynthesis lab: To study photosynthesis in the Understanding the process of photosynthesis,
leaves observing it happen in the lab, importance of
different conditions governing photosynthesis.
Experiment – 9: Mitosis Lab: Measurement of mitotic index and Understanding different phases of cell division,
duration of mitosis in the given plant tissue. different factors affecting it and preparation of
Observation of various stages of mitosis through slides to view mitosis in a plant meristematic
permanent slides. tissue.
Experiment – 10: Physiology Lab: Measurement of haemoglobin Blood group incompatibility, genetics behind
content in the human blood and determination of blood group inheritance, hemoglobin and its
blood group and Rh status. importance; Blood typing.
Note:
1. The order of experiments listed above may change depending on the availability of chemicals,
enzymes, and other requirements for a specific experiment.
2. The relevant reading material would be posted on Nalanda
3. ALL THE QUERIES TO THE INSTRUCTOR-IN-CHARGE SHOULD BE EMAILED TO
bitsbiocourse@gmail.com
Evaluation Scheme: Evaluations will be based on laboratory experiments and reading material posted on Nalanda.
S. No. Evaluation component Duration Date, time and Venue Weightage Nature of
(%) component
1. Day to day Evaluation - Daily Lab 35% OB
(Attendance+ Performance)
2. Quizzes - 20th Nov. and 18th Dec. 2021* 24% CB
4. Comprehensive Exam - TBA 41% CB
* Time will be announced one week before the quiz.
Notices: General notices will be displayed on Nalanda and section specific notices on google classroom.
Make up Policy:
No make ups will be granted for quizzes. Genuine cases may be considered only upon discussion with team.
However, it is mandatory to approach the instructor-in-charge via the section instructor.
Instructor-in-Charge
BIO F110
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BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani

First semester 2021-2022 Date: 25-09-2021

COURSE HANDOUT (PART-II)

Scope and Objective of the course:

Experiment No. Name of the Experiment Learning Outcome

Experiment – 1: Scientific Process Lab Understanding Scientific process, and its

Please Do Not Print Unless Necessary

Please Do Not Print Unless Necessary

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