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    Physioex Lab Report: Pre-Lab Quiz Results

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    Aurelia Raihani
    The document summarizes an experiment measuring the resting membrane potential of neurons under different conditions. In the control condition, the resting potential was -70mV inside the cell body and axon. When extracellular K+ was increased, the resting potential became less negative at -40mV, supporting the prediction. Changing extracellular Na+ did not significantly affect the resting potential, suggesting Na+ channels are mostly closed at rest.

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    Physioex Lab Report: Pre-Lab Quiz Results

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    Aurelia Raihani
    20 views8 pages
    The document summarizes an experiment measuring the resting membrane potential of neurons under different conditions. In the control condition, the resting potential was -70mV inside the cell body and axon. When extracellular K+ was increased, the resting potential became less negative at -40mV, supporting the prediction. Changing extracellular Na+ did not significantly affect the resting potential, suggesting Na+ channels are mostly closed at rest.

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    PhysioEx Exercise 3 Activity 1

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    The document summarizes an experiment measuring the resting membrane potential of neurons under different conditions. In the control condition, the resting potential was -70mV inside the cell body and axon. When extracellular K+ was increased, the resting potential became less negative at -40mV, supporting the prediction. Changing extracellular Na+ did not significantly affect the resting potential, suggesting Na+ channels are mostly closed at rest.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    20 views8 pages

    Physioex Lab Report: Pre-Lab Quiz Results

    Uploaded by

    Aurelia Raihani
    The document summarizes an experiment measuring the resting membrane potential of neurons under different conditions. In the control condition, the resting potential was -70mV inside the cell body and axon. When extracellular K+ was increased, the resting potential became less negative at -40mV, supporting the prediction. Changing extracellular Na+ did not significantly affect the resting potential, suggesting Na+ channels are mostly closed at rest.

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    11/25/2020 PhysioEx Exercise 3 Activity 1

    PhysioEx Lab Report


    Exercise 3: Neurophysiology of Nerve Impulses
    Activity 1: The Resting Membrane Potential
    Name: Aurelia Raihani Jannah
    Date: 25 November 2020
    Session ID: session-df5dca35-0ff5-9693-f676-2b5909de099f

    Pre-lab Quiz Results


    You scored 100% by answering 4 out of 4 questions correctly.

    1 What is the approximate concentration of K+ inside a typical cell (intracellular


    concentration)?

    You correctly answered: 150 mM.

    2 What is the approximate concentration of K+ outside a cell (extracellular


    concentration)?

    You correctly answered: 5 mM.

    3 What is the approximate concentration of Na+ inside a cell (intracellular


    concentration)?

    You correctly answered: 5 mM.

    4 What is the approximate concentration of Na+ outside a cell (extracellular


    concentration)?

    You correctly answered: 150 mM.

    Experiment Results
    Predict Question
    1 Predict Question: Predict what will happen to the resting membrane potential if the
    extracellular K+ concentration is increased.

    Your answer: The resting membrane potential will become less negative.

    Stop & Think Questions


    1 What is the polarity of the resting membrane potential (voltage)?

    You correctly answered: negative.

    2 What does it mean that the voltage just inside the membrane is negative? i
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    11/25/2020 PhysioEx Exercise 3 Activity 1

    You correctly answered: There are more negative charges than positive charges just
    inside the membrane.

    3 The membrane of most cells, including neurons, contains passive, open, K+ leak
    channels. Given the normal K+ concentrations and the resultant concentration
    gradient, which direction would K+ be expected to move (diffuse) through these leak
    channels?

    You correctly answered: out of the cell .

    4 What effect does increasing extracellular K+ have on the net diffusion of K+ out of the
    cell?

    You correctly answered: It decreases the net diffusion of K+.

    5 Which way would Na+ move across the membrane if there were open Na+ channels?

    You correctly answered: Na+ would diffuse into the cell.

    6 The membrane has open K+ channels, and changing extracellular K+ concentration


    results in a change in membrane potential. Changing the extracellular Na+
    concentration does not significantly change the membrane potential. What do your
    results suggest about the number or state (open or closed) of Na+ channels in the
    resting membrane of a neuron?

    You correctly answered: Na+ channels are mostly closed.

    Experiment Data

    Extracellular Fluid (ECF) Microelectrode Position Voltage (mV)


    Control Cell body, extracellular 0
    Control Cell body, intracellular -70
    Control Axon, extracellular 0
    Control Axon, intracellular -70
    High K+ Axon, intracellular -40
    High K+ Axon, extracellular 0
    High K+ Cell body, extracellular 0
    High K+ Cell body, intracellular -40
    Low Na+ Cell body, intracellular -72
    Low Na+ Cell body, extracellular 0
    Low Na+ Axon, extracellular 0
    Low Na+ Axon, intracellular -72

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    Post-lab Quiz Results


    You scored 67% by answering 2 out of 3 questions correctly.

    1 A negative membrane potential was recorded when the tip of the microelectrode
    was i
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    Your answer: just inside the cell body.

    Correct answer: both inside the cell body and inside the axon.

    2 Which of the following caused a change in membrane potential from -70 to -40 in the
    cell body?

    You correctly answered: an increase in extracellular K+.

    3 Which of the following has the most negative voltage?

    You correctly answered: between the inside of the axon and the outside of the axon
    with control K+ ECF.

    Review Sheet Results


    1 Explain why increasing extracellular K+ reduces the net diffusion of K+ out of the
    neuron through the K+ leak channels.

    Your answer:

    There is usually more K⁺ inside the cell relative to the extracellular. Increasing
    extracellular K⁺ reduces the concentration gradient, thus reducing net diffusion of K⁺.

    2 Explain why increasing extracellular K+ causes the membrane potential to change to


    a less negative value. How well did the results compare with your prediction?

    Your answer:

    Resting membrane potential is negative because the negative charge inside the cell is
    greater than the positive charge outside the cell. Increasing extracellular K+
    increases the positive charge outside the cell. This decreases the difference between
    the inside and outside of the cell.

    3 Explain why a change in extracellular Na+ did not significantly alter the membrane
    potential in the resting neuron?

    Your answer:

    A change in extracellular Na+ results in little change to resting membrane potential


    because the plasma membrane of a neuron is only slightly permeable to Na+
    because it contains relatively few Na+ leakage channels. This inhibits net diffusion of
    Na+ into or out of the cell.

    4 Discuss the relative permeability of the membrane to Na+ and K+ in a resting neuron.

    Your answer:

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    Membrane permeability of a resting neuron to K+ is four to five times greater than


    for Na+.

    5 Discuss how a change in Na+ or K+ conductance would affect the resting membrane
    potential.

    Your answer:

    The membrane has a low permeability to Na ions because there are very fee ma leak
    channels, and Na channels are closed a change in k+ conductance would have a
    greater effect on testing membrane potential than a change in Na+ conductance
    because the membrane Na more permeable to k+.

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