This document is the Slovenian standard SIST EN ISO 7346-2 from 1998 which is identical to the international standard ISO 7346-2 from 1997. ISO 7346-2 provides guidelines for determining the acute lethal toxicity of substances to freshwater fish, specifically the zebrafish (Brachydanio rerio), using a semi-static method where the test solutions are renewed every 24-48 hours.
This document is the Slovenian standard SIST EN ISO 7346-2 from 1998 which is identical to the international standard ISO 7346-2 from 1997. ISO 7346-2 provides guidelines for determining the acute lethal toxicity of substances to freshwater fish, specifically the zebrafish (Brachydanio rerio), using a semi-static method where the test solutions are renewed every 24-48 hours.
This document is the Slovenian standard SIST EN ISO 7346-2 from 1998 which is identical to the international standard ISO 7346-2 from 1997. ISO 7346-2 provides guidelines for determining the acute lethal toxicity of substances to freshwater fish, specifically the zebrafish (Brachydanio rerio), using a semi-static method where the test solutions are renewed every 24-48 hours.
This document is the Slovenian standard SIST EN ISO 7346-2 from 1998 which is identical to the international standard ISO 7346-2 from 1997. ISO 7346-2 provides guidelines for determining the acute lethal toxicity of substances to freshwater fish, specifically the zebrafish (Brachydanio rerio), using a semi-static method where the test solutions are renewed every 24-48 hours.
Water quality - Determination of the acute lethal toxicity of substances to a freshwater
fish (Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)) - Part 2: Semi-static method (ISO 7346-2:1996) iTeh STANDARD PREVIEW (standards.iteh.ai) Wasserbeschaffenheit - Bestimmung der akuten letalen Toxizität von Substanzen gegenüber einem Süßwasserfisch (Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae) - Teil 2: SemistatischesSIST EN ISO 7346-2:1998 Verfahren (ISO 7346-2:1996) https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998 Qualité de l'eau - Détermination de la toxicité aiguë létale de substances vis-a-vis d'un poisson d'eau douce (Brachydanio rerio Hamilton-Buchanan (Téléostei, Cyprinidae)( - Partie 2: Méthode semi-statique (ISO 7346-2:1996)
Ta slovenski standard je istoveten z: EN ISO 7346-2:1997
ICS: 13.060.70 Preiskava bioloških lastnosti Examination of biological vode properties of water
SIST EN ISO 7346-2:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno. SIST EN ISO 7346-2:1998
iTeh STANDARD PREVIEW
(standards.iteh.ai) SIST EN ISO 7346-2:1998 https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998 SIST EN ISO 7346-2:1998
iTeh STANDARD PREVIEW
(standards.iteh.ai) SIST EN ISO 7346-2:1998 https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998 SIST EN ISO 7346-2:1998
iTeh STANDARD PREVIEW
(standards.iteh.ai) SIST EN ISO 7346-2:1998 https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998 SIST EN ISO 7346-2:1998
INTERNATIONAL IS0 STANDARD 7346-2 Second edition 1996-06-I 5
Water quality - Determination of the
acute lethal toxicity of substances to a freshwater fish [ Brachydanio rerio iTeh Hamilton-Buchanan STANDARD PREVIEW (Teleostei, ’ Cyprinidae)] - (standards.iteh.ai) Part 2: SIST EN ISO 7346-2:1998 Semi-static method https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998
Qualit de I’eau - D6 termination de la toxicit aigu6 l&ale de substances
vis-A-vis d’un Poisson d’eau deuce [Brachydanio rerio Hamilton-Buchanan (T6lkos tei, Cyprinidaefi - Partie 2: M&hode semi-statique
Reference number IS0 7346-Z: 1996(E) SIST EN ISO 7346-2:1998
IS0 7346-2: 1996(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work of preparing International Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the International Electrotechnical Commission (I EC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. iTeh STANDARD PREVIEW ISO/TC 147, Water quality, Subcommittee (standards.iteh.ai) International Standard IS0 7346-2 was prepared by Technical Committee SC 5, Biological methods. SIST EN ISO 7346-2:1998 Thi‘S second edition cancels and replaces the first edition https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- (IS0 7346-2: 1984), which has been technically revised. 44c013b6456e/sist-en-iso-7346-2-1998 IS0 7346 consists of the following parts, under the general title VVater quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)]:
- Part I: Static method
- Part 2: Semi-static method
- Part 3: Flow-through method
Annexes A, B and C of this part of IS0 7346 are for information only.
0 IS0 1996 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. lnternationa I Organization for Standardization Case Postal e 56 l CH-121 1 Geneve 20 l Switzerland Printed in Switzerland
ii SIST EN ISO 7346-2:1998
0 IS0 IS0 7346-2: 1996(E)
Introduction The three parts of IS0 7346 describe methods of determining the acute lethal toxicity of substances to the zebra fish (Brachydanio rerio Hamilton-Buchanan) but it must be emphasized that the recommended use of the zebra fish does not preclude the use of other species. The methodologies presented here may also be used for other species of freshwater, marine or brackish water fish, with appropriate modifications of, for example, dilution water quality and the temperature conditions of the test. Within the three parts of IS0 7346, a choice can be made between static, semi-static and flow-through methods” The static test, described in IS0 7346-1, in which the solution is not renewed, has the advantage of requiring simple apparatus, although the substances in the test vessel may iTeh STANDARD PREVIEW become depleted during the course of the test and the general quality of the water may deteriorate. The flow-through method, described in (standards.iteh.ai) IS0 7346-3, in which the test solution is replenished continuously, over- comes such problems but requires the use of more complex apparatus. In the semi-static procedure, described in this part of IS0 7346, the test SIST ENare solutions ISOrenewed 7346-2:1998 every 24 h or 48 h, this method being a compro- https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- mise between the other two. 44c013b6456e/sist-en-iso-7346-2-1998 The flow-through method can be used for most types of substances, in- cluding those unstable in water, but the concentrations of the test sub- stance are determined wherever possible. The static method is limited to the study of substances whose tested concentrations remain relatively constant during the test period. The semi-static method can be used for testing those substances whose concentrations can be maintained satis- factorily throughout the test by renewal of the solutions every 24 h or 48 h. Special arrangements may be necessary for substances which are highly volatile. To assist in the preparation and maintenance of concentrations of sub- stances which may be lethal at concentrations close to that of their aqueous solubility, a small volume of solvent may be used, as specified in the methods.
... III SIST EN ISO 7346-2:1998
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(standards.iteh.ai) SIST EN ISO 7346-2:1998 https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- 44c013b6456e/sist-en-iso-7346-2-1998 SIST EN ISO 7346-2:1998
INTERNATIONAL STANDARD 0 IS0 IS0 7346-2: 1996(E)
Water quality - Determination of the acute lethal
toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] -
Part 2: Semi-static method
1 Scope with appropriate modification of the test conditions,
particularly with respect to the quantity and quality of iTeh STANDARD PREVIEW This part of IS0 7346 specifies a semi-static method the dilution water and the temperature.
for the determination (standards.iteh.ai)
of the acute lethal toxicity of stable, non-volatile, single substances, soluble in 2 Principle water under specified conditions, to a freshwater SIST EN fish ISO 7346-2:1998 [ Brachydanio rerio Hamilton-Buchanan (Teleostei, https://standards.iteh.ai/catalog/standards/sist/8ca4ed48-4cf7-4001-ad6b- Determination, under specified conditions, of the Cyprinidae) - common name, zebra 44c013b6456e/sist-en-iso-7346-2-1998 fish] in water of concentrations at which a substance is lethal to a specified quality. 50 % of a test population of Brachydanio rerio after exposure periods of 24 h, 48 h, 72 h and 96 h to that The method is applicable for assigning, for each test substance in the ambient water. These median lethal substance, broad categories of acute lethal toxicity to concentrations are designated the 24 h - LC50, Brachydanio rerio under the test conditions. 48 h - LC50, 72 h - LC50 and 96 h - LC50.
The results are insufficient by themselves to define The test is carried out in two stages: water quality standards for environmental protection. a) a preliminary test which gives an approximate in- The method is also applicable when using certain dication of the acute median lethal concentrations other species of freshwater fish as the test and serves to determine the range of concen- organisml). trations for the final test;
The method may be adapted for use with other b) a final test, the results of which alone are re- freshwater fish and marine and brackish water fish ported.
I) The following species of freshwater fish can be used, in addition to Brachydanio rerio, without modification to this part of IS0 7346.
