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    Reportviewinpdf - 2022-07-26T191041.034

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    Usman niaz
    The patient was tested for HCV RNA using real-time PCR and the result was detected at a titer of 223,540 IU/ml. HCV RNA was extracted from the patient's plasma sample using a mini spin column purification technique. The purified RNA was then amplified using a real-time PCR kit that is CE-IVD approved for diagnosis and detected simultaneously on a rotor gene and qPCR cycler. A single non-detective result should be repeated after a clinically significant interval as determined by clinicians due to viral load fluctuations. Test results may vary between laboratories due to different quality control policies and methods used.

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    Reportviewinpdf - 2022-07-26T191041.034

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    Usman niaz
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    The patient was tested for HCV RNA using real-time PCR and the result was detected at a titer of 223,540 IU/ml. HCV RNA was extracted from the patient's plasma sample using a mini spin column purification technique. The purified RNA was then amplified using a real-time PCR kit that is CE-IVD approved for diagnosis and detected simultaneously on a rotor gene and qPCR cycler. A single non-detective result should be repeated after a clinically significant interval as determined by clinicians due to viral load fluctuations. Test results may vary between laboratories due to different quality control policies and methods used.

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    The patient was tested for HCV RNA using real-time PCR and the result was detected at a titer of 223,540 IU/ml. HCV RNA was extracted from the patient's plasma sample using a mini spin column purification technique. The purified RNA was then amplified using a real-time PCR kit that is CE-IVD approved for diagnosis and detected simultaneously on a rotor gene and qPCR cycler. A single non-detective result should be repeated after a clinically significant interval as determined by clinicians due to viral load fluctuations. Test results may vary between laboratories due to different quality control policies and methods used.

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    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    56 views1 page

    Reportviewinpdf - 2022-07-26T191041.034

    Uploaded by

    Usman niaz
    The patient was tested for HCV RNA using real-time PCR and the result was detected at a titer of 223,540 IU/ml. HCV RNA was extracted from the patient's plasma sample using a mini spin column purification technique. The purified RNA was then amplified using a real-time PCR kit that is CE-IVD approved for diagnosis and detected simultaneously on a rotor gene and qPCR cycler. A single non-detective result should be repeated after a clinically significant interval as determined by clinicians due to viral load fluctuations. Test results may vary between laboratories due to different quality control policies and methods used.

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    Patient Number:

    0155454-1001
    0155454-1001

    Lab Number: 22-072022-2817


    22-072022-2817

    M.R. No: 1 Registration Date: 22-Jul-2022 12:45 pm


    Patient Name: NAZIA BIBI Registration Location: Allied Lahore Lab
    Father/Husband Name: Approved Date: 22-Jul-2022 6:44 pm
    Age / Sex: 30 Year(s)/Female Reporting Location: CC MIAN MEER
    NIC/Passport No: Sampling Location: Brought to The Lab
    Track Online
    Mobile: Reference: CC MIAN MEER
    Address: Consultant:

    MOLECULAR BIOLOGY REPORT

    TEST: HCV RNA BY REAL-TIME PCR (QUANTITATIVE)


    RESULT: DETECTED
    TITRE: 223,540 IU/ml
    Interpretation Analytical Sensitivity of Assay – 30 IU/ml
    Linearity Limit of Assay – 3.0 x 108IU/ml
    Specificity of Assay: 100% for all prevalent geno & sub types of Hepatitis C Virus.
    Methodologies
    RNA Extraction & Purification:

    Hepatitis C virus RNA is isolated from plasma sample of patient, using mini spin column purification technique of
    Viral NA extraction kits manufactured by Vivantis Technologies Malaysia. Internal Control provided in Real Time
    PCR kit is added during procedure of extraction at the same time when sample material is added. This Internal
    Control will help to check possible NA loss during extraction or possible PCR inhibition during Amplification
    process. Thus IC behavior will ensure results authenticity towards false negativity.

    Amplification & Real Time Detection:

    Purified viral NA is amplified using Real Time PCR kits Anatolia Gene Works Turkey, these kits are CE -IVD
    approved for human diagnosis. Real Time detection of amplified HCV RNA and Internal control is simultaneously
    done on Real Time PCR system Rotor Gene Qiagen Germany & qPCR Cycler MIC BMS Australia.
    Comments
    The viral load of hepatitis C virus in blood fluctuates with viral latency and may be below sensitivity limit of the assay, so
    a single non detective result should be repeated after clinical significance interval or as decided by the clinicians.

    The lab tests performed in different laboratories may not be compared with each other as each lab has its own quality
    control policy and use of different methods. This report is not valid to be presented in any court of law.

    This is a digitally verified report and does not require any signature

    Dr. Sara Iqbal Ch DR HUMA SHAHZAD M. Waqar DR.SHAHILA JALEEL


    M.B.B.S, D.C Path MBBS:(PUNJAB) B.Sc (MLT Honors) M.B.B.S -F.C.P.S (Histo-Pathology)
    F.C.P.S (Histo-Pathology) M.PHIL.(HAEMATOLOGIST) Medical Lab Technologist Consultant Histo & Cyto
    Consultant Histo & Cyto CONSULTANT HAEMATOLOGIST & M.Phil (Molecular Biology) Pathologist
    Pathologist CLINICAL PATHOLOGIST Chief Technologist associate professor Histo. Sheikh
    Zayed FPGMI Lahore

    Plaza No. 11, Imperial Market, Paragon City Barki Road, Lahore. Contact: 04237193640, 03464675250 www.alliedlahorelab.com, info@alliedlahorelab.com

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