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Module 1 Lessons 4-5 Activities

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Name: ___________________________________________________

Gr./Sec.: _________________________________________________

Activity No. 1: Is it Pure?

Directions: Perform the following activity and summarize your observations.

Materials:
4 small clear containers baking soda spoons
White sugar distilled water masking tape
Baking powder measuring cup pen
Flour teaspoon

Procedure:
a. Label the containers with the names of the four solids (white sugar, baking powder, flour, and
baking soda).
b. Pour one-half cup of water into each of the four containers.
c. Add one-half teaspoon of the white solid to its corresponding container. Do not stir. Take not
of any reactions between the solid and the water.
d. Gently stir the contents of each container for at least 45 seconds, and observe what happens
to the white solids.
e. Summarize your observations below.

Observations:

Questions:
1. Which materials (starting materials and products) do you think are examples of pure
substances? Enumerate all.
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2. How about mixtures? Enumerate all.


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3. Why do you say that your answers in no. 2 are mixtures?

Activity 1: Paper Chromatography


Perform the following activity.

Objectives:
 Separate the different pigments of a candy coating using paper chromatography
 Determine the pigments of a candy coating that have the same nature of polarity as the
mobile and stationary phases used

Materials:
Different colors of coated candies (e,g., M & M) clear plastic tape
Ethyl alcohol pencil
Water beaker
Filter paper 2 watch glass
Ruler toothpick
Clear plastic wrap

(Note: Feel free to modify materials that are not available.)

Procedure:
1. Cut the filter paper following the dimensions in the illustration. The width of the filter paper
should be about the diameter of the beaker. This is your chromatogram paper.

2. Draw a pencil line one centimeter from the bottom of the strip. This line is the “start line”.
3. Sample preparation. Take one candy for each color. Place the candies farther apart on a
watch glass. On each candy, place one to two drops of water. Let the samples stand until
sample spotting is to be done.
4. Using a toothpick, make a tiny dot of each color extract along the start line. The dots should
be farther apart.
5. Pour ethyl alcohol into the beaker, just enough to cover the bottom. Then, place another strip
of filter paper (half the size of the chromatogram paper) in the beaker so that it sticks to the
side of the beaker. Allow the ethyl alcohol to move up this paper. Cover the beaker with a
clean watch glass.
6. When the entire smaller paper has become wet, carefully place the chromatogram paper with
samples in the beaker, making sure that the start line is kept above the ethyl alcohol.
7. Hang the chromatogram paper on a pencil and hold it with plastic tape. Cover the beaker with
the plastic wrap.
8. Observe and describe how the ethyl alcohol “climbs” up the paper, dragging the samples with
it. Watch the colored spots go up the strip and possibly separate into individual components.
Write your observations below.
9. Remove the chromatogram paper from the beaker when the solvent front reaches the “stop
line”.
10. Mark where the solvent front is and where the centers of the colored spots are. Use a pencil
in marking the paper.
11. Allow the chromatogram to dry. Attach it below and submit to your teacher.

Observations:
How did the appearance of the filter paper change during the progress of the chromatographic
procedure?

Attach photo here:

Conclusion:

Post-Activity Questions:
1. Is the candy coating pigment a mixture? Why? Why not?
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2. How useful is paper chromatography?


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