Guava Chemistry Project

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OBJECTIVE

The objective of this project is to study of


oxalate ion in present guava fruit.
INTRODUCTION

Guava is a common sweet fruit found in India and


many other places which contain about 100
species of tropical shrub
On ripening it turns yellow in color. Rich in
vitamin C, this fruit is a rich source of oxalate
ions whose content varies during different
stages of ripening.
It is a carboxylic acid, primarily found in plants
and animals. It is not an essential molecule and
is excreted from our body, unchanged.
Our body either produces oxalate on its own or
converts other molecules like vitamins C to
oxalate. External sources like food also
contributed to the accumulation of oxalate in our
body.
DAILY LIFE APPLICATION OF GUAVA

1. IMMUNITY BOOSTER
Vitamin C present in guava helps improve immunity
and protects us against common infection and
pathogens.

2. DIABETES-FRENDLY
Due to rich fiber content and low glycaemic index,
guavas prevent the development of diabetes.

3. HEART HEALTHY
Guava improve the sodium and potassium balance of
the body ,thereby regulating blood.
THEORY

Oxalate ions are extracted from the fruit by


boiling pulp with dilute H2SO4.
The oxalate ions are estimated volumetrically, by
titrating the solution with KMnO4 solution.
PROCEDURE

 Weighted 50g of fresh guava and crushed it to a fine pulp


using pestle and mortal.

 Transferred the crushed pulp to a beaker and added


about 50 ml dilute H2SO4 to it.

 Boiled the content for about 10 minutes. Cooled and


filtered the contents in a 100 ml measuring flask.

 Made up the volume 100 ml by adding ample amount of


distilled water.

 Took 20 ml of the solution from the flask and added 20 ml


of dilute sulphuric acid to it.

 Heated the mixture to about 60oC and titrated it against


(N/10) KMnO4 solution taken in a burette till the end point
had an appearance of pink color.

 Repeated the above experiment with 50g of 1 day, 2day


and 3 - day old guava fruits.
OBSERVATION

 Weight of guava fruit for each time was 50 g.

 Volume of guava extract taken for each titration


was 10 ml.

 Normality of KMnO4 SOLUTION WAS (1/10).

 End point: colour changes to pink.

Guava Burette Final Volume Concordant


Solution reading reading of Reading
initial KMnO4
Raw 150 18 132
Semi 150 13 137
136.06
ripened
Ripened 150 10.8 139.2
CALCULATION
1. For raw guava
 N1V1 =N2V2
 N1 * 10= (1/10) * 132
 N1 = 132/100 =1.32
Strength of oxalate in fresh
guava extract
= normality * Eq. mass of oxalate
ion
= 1.32/100* 44g / liter of diluted
extract
= 0.581 g L-1
2. For semi ripened guava (1 day old)
Strength of oxalate in one day old
guava extract
= 1.37/ 100*44g / liter of diluted
extract
= 0.6.3 g L-1
3. For ripened guava
Strength of oxalate in ripened guava
extract
= 1.39 / 100* 44g / liter of diluted
extract
= 0.612 g L-1
RESULT
a) The normality of oxalate ion of:
 Fresh guava solution is =1.32 N
 Semi =ripen guava solution is = 1.37 N
 Ripened guava solution is = 1.39 N
b)The strength of oxalate ion of:
 Fresh of guava solution is =0.58 gL-1
 Semi – ripen guava solution is = 0.60gL-1
 Ripened guava solution is = 0.61 gL-1
CONCLUSION

 The content of oxalate ion in guava


was found to be 59.67 percent,
which is close to the literature value
of 60 percent.

 It was also noticed that the content


of oxalate ion grows with ripening of
guava.
BIBLIOGRAPHY

 WWW.Wikipedia.com
 WWW.encyclopedia.com
 http: //WWW.cbseportal.com
 book.google.co.in
CONTENT
 Objective
 Introduction
 Application
 Theory
 Requirements
 Procedure
 Observation
 Calculation
 Result
 Conclusion
 Bibliography
REQUIREMENTS
100 ml measuring flask burette Beaker Filter

Funnel Paper weighing machine

KMnO4

dil H2SO4

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