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    Fuji Dri-Chemi Analyzer

    Uploaded by

    William Lusekelo
    This document provides the procedure for performing electrolytes (Na+, K+, Cl-) tests using a Fuji Dri-Chem analyzer at Rabininsia Memorial Hospital Laboratory. It outlines the purpose, scope, responsibilities, principles, equipment, reagents, sample requirements, procedure steps, limitations, and quality control for the electrolyte test. The procedure is approved by the laboratory manager and laboratory quality officer to ensure accurate and consistent electrolyte testing is performed.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Fuji Dri-Chemi Analyzer

    Uploaded by

    William Lusekelo
    62 views6 pages
    This document provides the procedure for performing electrolytes (Na+, K+, Cl-) tests using a Fuji Dri-Chem analyzer at Rabininsia Memorial Hospital Laboratory. It outlines the purpose, scope, responsibilities, principles, equipment, reagents, sample requirements, procedure steps, limitations, and quality control for the electrolyte test. The procedure is approved by the laboratory manager and laboratory quality officer to ensure accurate and consistent electrolyte testing is performed.

    Original Description:

    Fuji Dri Chem analyzer operating procedure

    Original Title

    Fuji Dri-Chemi analyzer

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    This document provides the procedure for performing electrolytes (Na+, K+, Cl-) tests using a Fuji Dri-Chem analyzer at Rabininsia Memorial Hospital Laboratory. It outlines the purpose, scope, responsibilities, principles, equipment, reagents, sample requirements, procedure steps, limitations, and quality control for the electrolyte test. The procedure is approved by the laboratory manager and laboratory quality officer to ensure accurate and consistent electrolyte testing is performed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    62 views6 pages

    Fuji Dri-Chemi Analyzer

    Uploaded by

    William Lusekelo
    This document provides the procedure for performing electrolytes (Na+, K+, Cl-) tests using a Fuji Dri-Chem analyzer at Rabininsia Memorial Hospital Laboratory. It outlines the purpose, scope, responsibilities, principles, equipment, reagents, sample requirements, procedure steps, limitations, and quality control for the electrolyte test. The procedure is approved by the laboratory manager and laboratory quality officer to ensure accurate and consistent electrolyte testing is performed.

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    Laboratory Name: Rabininsia Memorial Hospital Laboratory

    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test


    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    PROCEDURE FOR ELECTROLYSTES( Na+, K+, Cl-) TEST USING FUJI


    DRI-CHEM ANALYZER.

    Name Designation Signature Date


    Prepared by:

    Reviewed by: William M Lusekelo Laboratory Quality Officer

    Approved by: Laboratory Manager

    @RMHL Page 1
    Laboratory Name: Rabininsia Memorial Hospital Laboratory
    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test
    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    1. Purpose
    This procedure provides Instruction for performing electrolytes (Na+, K+, Cl-) test using
    FUJI DRI-CHEM ANALYZER.

    2. Scope
    This procedure applies instructions and processes to be used at RMH Laboratory during
    electrolytes (Na+, K+, Cl-) test performance.

    3. Responsibility
    All laboratory staffs authorized to perform(Na+, K+, Cl-) testare responsible for
    implementing this procedure. The Head of Clinical Chemistry unit is responsible for
    ensuring the effective implementation and maintenance of this procedure.

    4. Definition and abbreviation


    SOP Standard Operating procedures
    RMH Rabininsia Memorial Hospital
    N/A Not Applicable
    +
    Na Sodium
    +
    K potassium
    -
    Cl Chloride

    5. Principle of the Procedure


    50 µL of reference fluid and 50 µL of whole blood, plasma or serum is deposited on a
    Fuji Dri-Chem Slide Na-K-Cl at the same time on the reference side and the sample side
    respectively. After depositing the reference fluid and the sample spread along the
    distribution device and also toward each other on the special thread bridge to form a
    stable ionic junction. A differential potential is generated between the half-cells, the
    potential difference is proportional to the logarithm of each ion concentration ratio of the
    two fluids. The slide is incubated for a fixed time in the FUJI DRI-CHEM ANALYZER
    and the potentiometric difference between the reference and the sample is measured. The
    potentiometric value is then converted into of the electrolyte’s concentration using a
    calibration curve preinstalled in the analyzer.

    @RMHL Page 2
    Laboratory Name: Rabininsia Memorial Hospital Laboratory
    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test
    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    6. Equipment and reagents


    Reagents Supplies Equipment

     Gloves
     Fuji Dri-Chem  Plain vacutainer tubes  FUJI DRI-
    Reference Fluid  Heparinized CHEM
    vacutainer tube ANALYZER
     Fuji Dri-Chem Slide

    7. Sample and container type


     Heparin can be used as anticoagulant. When using heparin, less than 10 units of
    heparin-Na or less than 50 units of heparin-Li should be used per 1 mL of whole
    blood. Do not use EDTA salt, sodium floride, citric acid, oxalic acid and
    monoiodoacetic acid
     Measure the sample immediately after blood drawing. When samples are to be left
    standing, take the following care. Measure the sample within an hour
     Keep the sample at room temperature. Potassium concentration deviates
    higher when the blood sample is cooled in a refrigerator
     Flopping the sample tube upside down gently prior to the measurement
     Avoid using plasma or serum with precipitate such as fibrin
     Do not use hemolytic plasm or serum.

    8. Environmental and safety controls


     Handle all specimens as if they contain infectious agents, observe established
    precautions against microbiological hazards throughout testing and follow the
    standard procedures for proper disposal of specimens.
     Use appropriate personal protective equipment.
     Room temperature monitoring is done twice daily

    @RMHL Page 3
    Laboratory Name: Rabininsia Memorial Hospital Laboratory
    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test
    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    9. Maintenance
    Refer to Fuji Dri-Chem maintenance procedure RMHL/PR/CC 003

    10. Calibration
    N/A

    11. Quality Control


     On daily basis run control material before performance of any patients sample.
     When the results obtained are outside the expected range, investigate the cause.

    12. Procedural steps


    i. Set slides on FUJI DRI-CHEM ANALYZER.
    ii. Set a sample tube in the specified sample rack.
    iii. Set the reference fluid in the specified position.
    iv. Input a sequence No. and a sample ID if appropriate.
    v. Press the ‘START’ key to initiate testing.
    CAUTION; Use immediately after opening the individual package
    For further details of operation procedure, consult ‘INSTRUCTION
    MANUAL’ for FUJI DRI-CHEM ANALYZER.

    13. Calculations.
    Calculations are automatically done by the analyzer

    14. Limitations.
    Known interfering substances
     Cationic surfactant as benzalkonium chloride and alcohol give a plus bias
     Br- on I- contained in the sample may affect the Cl-.
     Sample from patients overdosed with aspirin, may show plus bias of the Cl- data.
     In case of the sample of which sodium ion (Na+) and Chloride ion (Cl-) are both low
    in concentration, it is known that the value of Na+ measured by colorimetry method

    @RMHL Page 4
    Laboratory Name: Rabininsia Memorial Hospital Laboratory
    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test
    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    may be less than the flame photometry method. This is because part of free Na+
    become non dissociative NaHCO3 by binding with HCO3.

    15. Reporting
    Results are released with reference ranges which aid in the correct interpretation

    16. Result interpretation


    The analyzer is integrated to give out printout of results with normal ranges.

    17. Biological reference interval.


    Na+ 135.00-145.00 mmol/L
    K+ 3.50-5.10 mmol/L
    -
    Cl 96.00-108.00 mmol/L

    18. Performance characteristics


    Refer to Fuji Dri-Chem Verification and Validation RMHL/PR/CC 004

    19. Attachment
     SOP review and updating log
     Staff acknowledgement Log

    20. Supporting documents


    Fiji Dri-Chem maintenance log RMHL/FM/CC 003

    21. References
    Fuji Dri-Chem Clinical Chemistry Analyzer Instruction Manual.

    SOP Review and Updating Logs


    REVIEWERS NAME NATURE OF CHANGE SIGNATURE DATE

    @RMHL Page 5
    Laboratory Name: Rabininsia Memorial Hospital Laboratory
    Document Title: Procedure For Electrolystes ( Na+, K+, Cl-) Test
    Using Fuji Dri-Chem Analyzer.
    Document number. RMHL/SOP/CC 003
    Version number : 01
    Section: Clinical Chemistry
    Effective Date:01/04/2018

    Staff acknowledgement Log


    I have read, understood and agree to follow the procedure as documented.
    Name of staff Signature Date

    @RMHL Page 6

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