More Pink . Less Zinc . - Mauro Di Benedetto

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS
35ᵗʰ Annual Convention

05 - 07 Oct 2022 | 9am - 2pm PHT
MORE PINK, LESS

ZINC
Mauro di Benedetto and Ricardo Neto
Kemin Industries
CONTENTS
• Weaning challenges
• The use of ZnO at weaning
• Successfully weaning piglets: a holistic approach to
take, now and in future
• Solutions for ZnO at high doses
• Conclusions

35ᵗʰ Annual Convention | 05 - 07 Oct 2022 | 9am - 2pm PHT
WEANING CHALLENGES
Weaning is defined as «the progressive reduction in the

rate of milk transfer from mother to young».
Accompanied by and increasing intake of solid food by
the offspring and profound behavioral changes in the
parent offspring relationship. (Martin, 1984)

WEANING CHALLENGES
Natural weaning
•Natural weaning lasts around three months.
•Sow milk continues to be available to the piglet
•The piglet explores novel foods, and its gut adapts
microbiologically and immunologically to new food sources.
•Solid food contains around 200 g DM/kg.
•No change of environment at weaning.
•Piglets continue to sleep with sows even after weaning.

WEANING CHALLENGES
Stress • Lack of nutrients
(weaning, • Oxidation ←→ inflammation
thermal, • DNA damage
Cell
transport..)
damage
and
Reduced oxidative
feed stress
intake Intestinal
mucosal
damage
Reduced
Intestinal
intestinal
infections
integrity
(PWD)
WEANING CHALLENGES
Post weaning diarrhoea
• Colibacillosis causes huge economic
loss to the pig industry
• Salmonelosis each year.
• Swine Dysentery • First two weeks post
• PRRS
• Brachyspira weaning
hyodysenteriae • SI
• Mortality 20 – 30%
• Spirochaetal colitis • PCV2 • Poor body condition
• Brachyspira pilosicoli • Rotavirus of survivors
• Proliferative enteritis • Vaccination • Poor growth of
• Lawsonia s survivors
intracellularis
ZINC OXIDE AND WEANING
Solution until 26 June 2022 (EU)
• ZnO at high doses: Under prescription at ̴ Concerns about
3.0 kg/t (2500 ppm Zn) for 14 days to environmental
treat PWD accumulation of minerals
Antimicrobial effects resulting from high dietary
levels of inorganic zinc and
Enzyme secretion and digestibility copper
enhancement
Fear of ZnO used at high
Improved intestinal morphology doses leading to the
development of resistance.
Immune system
Liu P. Et al (2013), Kromm V. (International Pig Topics, Vol 23).
,
A HOLISTIC APPROACH TO SUCCESSFUL
WEANING
Various aspects must be considered to safeguard a
healthy and profitable piglet production post-weaning:
1. Management……
2. Healthy, efficient immune system.
3. Digestibility.
4. Addressing dysbiosis due to changing gut conditions.
5. Healthy gut microbiome.
6. Supporting piglet’s gut integrity.
BR-21-19898
SUCCESSFULLY WEANING PIGLETS
ß-1,3
glucans DIGESTIVE SUPPORT
Butyric acid
IMMUNE SUPPORT
INTESTINAL HEALTH
ENTEROBACTERIACEAE
PB6 B. subtilis CONTROL
INTESTINAL INTEGRITY
Organic acids
Enzymes and
biosurfactant
s
Zinc oxide | Antimicrobial effect
● Can reduce enteric gram-negative infections (e.g. E. coli

and Salmonella sp.).
● Mode of action may not be a direct antimicrobial effect;
ZnO was proven to inhibit bacterial adhesion to cells.

ß-1,3
Butyric acid glucans
ENTEROBACTERIACEAE
CONTROL
PB6 B. subtilis
Organic acids
Enzymes and
biosurfactant
PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS s
CONTROL UNDESIREABLE BACTERIA
Technical
Ingredients
Slow-Release Organic Acids
Functional Ingredients EO’s Slow-Release
EO’s
Calcium Formate
Flavour Functional
Ingredients
Citric
Cinnamon Acid
Oregano
Citrus
Thyme
Capsicum
Slow-Release Organic Acid

Product Composition

CONTROL UNDESIREABLE BACTERIA
✔ Where do potential pathogens cause
1. Acid digesta from the disease?
stomach goes into the
duodenum.
✔ Where must the acid reach?
2. pH is detected by epithelial
cells that produce secretin
which signals to the 2 3
pancreas. 4
1
3. Pancreas release sodium
bicarbonate to dissociate
and neutralise acid.
4. Neutral digesta continues
through the digestive tract
ß-1,3
INTESTINAL HEALTH
PB6 B. subtilis
Organic acids
Enzymes and
biosurfactant
PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS s
MICROBIOME SUPPORT
Bacillus sp. PB6 shows 4 different modes of action:
1.Direct inhibition of Clostridium spp.

2.Anti-inflammatory effect
3.Stimulates the beneficial bacteria
4.Increases the diversity of the intestinal microbiome
Clinical Nutrition 31 (2012) 987-994, Spores from two distinct colony types of the strain Bacillus subtilis PB6
substantiate anti-inflammatory probiotic effects in mice, Benoît Foligné , Eric Peys , Jan Vandenkerckhove, Johan
Van hemel, Joëlle Dewulf, Jérôme Breton, Bruno Pot.

Zinc oxide | Improved intestinal morphology
•Zinc is linked with the maintenance or function

gastrointestinal tract.
•Pharmacological levels of ZnO increase proliferation of
enterocytes and villi:crypt ratio.
•ZnO improves tight-junction expression, which indicates
reduced intestinal permeability and better intestinal
integrity.

ß-1,3
PB6 B. subtilis
Organic acids
INTESTINAL
Enzymes and INTEGRITY
biosurfactant
s
BUTYRIC ACID CAN HELP MAINTAIN
•Enterocyte (epithelial cells) preferred energy source1
a
•Stimulates the proliferation, differentiation and

maturation of enterocytes
•Induces mucous production
•Upregulates tight junction formation
1
Guilloteau et al., 2010. Nutrition research reviews.
2
Leonel, A. J., & Alvarez-Leite, J. I. (2012). Current Opinion in Clinical Nutrition and Metabolic Care.
9
Gaudier, E., et al. Am J Physiol Gastrointest Liver Physiol, 2004. 287:G1168.
.

BUTYRIC ACID CAN HELP MAINTAIN
50% Ca-Butyrate encapsulated by Spray Freezing a
Technology
Overcome problems of unprotected Butyrate
No smell
Easy handling
Stable
Resistant to mixing and pelleting
1
Guilloteau et al., 2010. Nutrition research reviews.
2
Leonel, A. J., & Alvarez-Leite, J. I. (2012). Current Opinion in Clinical Nutrition and Metabolic Care.
9
Gaudier, E., et al. Am J Physiol Gastrointest Liver Physiol, 2004. 287:G1168.
.
Zinc oxide | Immune modulation
•Zinc oxide can reduce the expression of several
pro-inflammatory cytokines while improving the
transcription of the anti-inflammatory cytokine.
•Immune modulatory effect regarding intestinal
inflammation when supplementing piglets with
pharmacological levels of ZnO.

ß-1,3 glucans
Butyric acid IMMUNE SUPPORT
PB6 B. subtilis
Organic acids
Enzymes and
biosurfactant
s
IMMUNITY o Feed material (EU) – Dried algae
o >50% beta-glucan
o The only algae (Euglena) derived
beta-glucan
o Resistant to processing
conditions (heat stable)
o No risk of accidental overdosing
and variable results due to
standardisation

Pharmacological Zinc oxide | Enzyme
secretion and digestibility enhancement
•Stimulates secretion of superoxide dismutase
(catalyses the elimination of free radicals) in the
stomach.
•Stimulates secretion of ghrelin (“hunger hormone”) by
enteroendocrine cells; Ghrelin also enhances muscle
protein synthesis and cell proliferation.
•Increases the activity of several pancreatic proteases
(increases nutrient digestibility).
ß-1,3 DIGESTIVE SUPPORT

PB6 B. subtilis
Organic acids
Enzymes and
biosurfactants
OUR ZINC OXIDE PROGRAM
ß-1,3
Butyric acid glucans DIGESTIVE SUPPORT
IMMUNE SUPPORT
INTESTINAL HEALTH
ENTEROBACTERIACEAE
Organic acids
INTESTINAL
INTEGRITY
Enzymes and
biosurfactants
FIELD EXPERIENCE 1

FIELD EXPERIENCE – STUDY 1
Benefits of zinc oxide replacement in Pre-starter and
Starter diets for piglets
Prestarter out Prestarter in
1.5 kg/ton slow-release butyric acid

3 kg/ton zinc oxide (2400 ppm Zn) 5 kg/ton slow-release organic acids blend
3 kg/ton free organic acids 2x108 CFU/kg B. subtilis
Starter in
Starter out 1 kg/ton slow-release butyric acid
3 kg/ton zinc oxide (2400 ppm Zn) 4 kg/ton slow-release organic acids
3 kg/ton free organic acids blend
2x108 CFU/kg B. subtilis

EPRE-19-1083
neither group showed diarrhoea

35ᵗʰ Annual Convention | 05 - 07 Oct 2022 | 9am - 2pm PHT EPRE-19-1083
Trial 1 Starter Phase Trial 2 Starter Phase
zinc oxide alternative zinc oxide alternative
Initial number of animals 80 79 86 86
Initial average weight kg/animal 9.90 9.86 10.97 10.90
Mortality and reject (head) 5 1 13 1
Final weight kg 1463 1895 1638 2208
Additional weight with mortality (kg) 715.6 1120.8 867 1287
Average weight (kg) 19.5 24.3 22.4 26
Total feed consumption (kg) 1165 1534 1439 1767
FCR kg/kg 1.63 1.37 1.66 1.37
Cost of feed (at 0.36 €/kg) 419 552 518 636
Value of piglets for sale (at €1.52 per kg) 2224 2880 2490 3356
Economic benefit per piglet at start compared to zinc
0 6.91 0 8.70
oxide (€)
35ᵗʰ Annual Convention | 05 - 07 Oct 2022 | 9am - 2pm PHT EPRE-19-1083
FIELD EXPERIENCE 2

FIELD EXPERIENCE –
STUDY 2 Submitted
• 1,564 weaned piglets of 23 days of age (average 6.36 kg).

• Control group, fed a three-phase postweaning feeding program.
• Treated group supplemented with ß-1,3 glucans in combination
biosurfactant.
• Duration of 49 days.
• Growth performance mortality and removals were recorded. Feed
and water were provided ad libitum.

35ᵗʰ Annual Convention | 05 - 07 Oct 2022 | 9am - 2pm PHT SD-21-24436
FIELD EXPERIENCE –
STUDY 2 Submitted
Legend: BG – Beta-1,3 glucan

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS BS – Biosurfactant lysolecithin SD-21-24436
FIELD EXPERIENCE 3

Trial design Submitted
• Control (n=538) control diet
• SR-Elite group (n=534)

○ SR- Elite group feed supplemented with 4 kg/t of slow-release organic acid blend product
and 750 g/t of B. subtilis PB6 (3 x 108 CFU/kg of feed). Benzoic acid and calcium formate
were also used at a similar dose level compared to the standard diet (0.5% for each
product). An organic acid blend was also supplied in the drinking water of the piglets fed
the reformulated diet.
Parameters
• Data: Fecal score, body weights and feed intake
• Calculated: Average daily gain (ADG) and Feed Conversion Ratio (FCR;
FUp per kg gain)
Kemin internal refence: SD-21-24459
PROVEN IN THE FIELD
Submitted
Reformulated diet
Day ZnO diet (OA + PB6) Diet BW x diet
Body weight
Initial body weight (kg/pig)y 1 4.2 (±0.5) 4.1 (±0.6) na na na
Intermediary body weight
14
(kg/pig)y 6.6 (±0.8) 6.9 (±0.8) na na na
Final Body weight (kg/pig)y 43 22.8 (±1.8) 22.6 (±2.0) na na na
1-14 days after weaning

ADG (g/d/pig)x 1-14 173 198 0.13 0.007 37
FI (g/d/pig)x 1-14 265 275 0.53 ns 36
FCRx 1-14 1.51 1.4 0.03 <0.001 0.16
Kemin internal refence:

Legend: OA – Organic acid blend SD-21-24459
PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS PB6 – B. subtilis PB6 strain

PROVEN IN THE FIELD Submitted
Reformulated diet
Day ZnO diet (OA + PB6) Diet BW x diet
Body weight
Initial body weight (kg/pig)y 1 4.2 (±0.5) 4.1 (±0.6) na na na
Intermediary body weight (kg/pig)y 14 6.6 (±0.8) 6.9 (±0.8) na na na
Final Body weight (kg/pig)y 43 22.8 (±1.8) 22.6 (±2.0) na na na
ADG (g/d/pig)x 1-14 173 198 0.13 0.007 37
FI (g/d/pig)x 1-14 265 275 0.53 ns 36
FCRx 1-14 1.51 1.4 0.03 <0.001 0.16
ADG (g/d/pig)x 14-43 549 533 0.5 ns 57
FI (g/d/pig)x 14-43 820 741 0.25 ns 80
FCRx 14-43 1.43 1.38 0.21 ns 0.09
ADG (g/d/pig)x 1-43 427 424 0.85 ns 38
FI (g/d/pig)x 1-43 620 599 0.32 ns 49
FCRx 1-43 1.44 1.38 0.03 ns 0.07
Kemin internal refence:

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS SD-21-24459
35ᵗʰ Annual Convention | 05 - 07 Oct 2022 | 9am - 2pm PHT Legend: OA – Organic acid blend PB6 – B. subtilis PB6 strain
OUR SOLUTION

OUR ZINC OXIDE PROGRAM
ß-1,3
Butyric acid glucans DIGESTIVE SUPPORT
IMMUNE SUPPORT
INTESTINAL HEALTH
ENTEROBACTERIACEAE
Organic acids
Enzymes and
biosurfactant
s
PROPOSED SOLUTION
Recommendations
A holistic approach is crucial:
• Correct feeding and nutrition • Organic acid blend
• Management practices (weaning Pre-Starter • B. subtilis PB6
age/weight) and Starter • ß-1,3 glucan
• Biosecurity • Butyric acid
• Hygiene
• Environment
1% mortality = 1€ extra production
cost / produced pig
THANK YOU
Acknowledgments
Dr. Susanne Kirwan Szabolcs Tóth Dr. Alex de Leon
Technical Service Manager, Head of Technical Services
Global Technical Service Manager
Monogastric Kemin Animal Nutrition and
Intestinal Health Kemin Industries
Kemin Animal Nutrition and Health, Asia Pacific
Health, EMENA
Ricardo Neto
Technical Service Manager, Dr. Valentine Van Hamme Edison Roxas
Health Business Manager, Technical Service Manager
Kemin Animal Nutrition and Intestinal Health Kemin Animal Nutrition and
Health, EMENA Kemin Animal Nutrition and Health, Health, Asia Pacific
EMENA


More Pink . Less Zinc . - Mauro Di Benedetto

Uploaded by

Copyright:

Available Formats

More Pink . Less Zinc . - Mauro Di Benedetto

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

More Pink . Less Zinc . - Mauro Di Benedetto

Uploaded by

Copyright:

Available Formats

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

35ᵗʰ Annual Convention

MORE PINK, LESS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

Weaning is deﬁned as «the progressive reduction in the

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

● Can reduce enteric gram-negative infections (e.g. E. coli

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

Slow-Release Organic Acid

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

Bacillus sp. PB6 shows 4 different modes of action:

1.Direct inhibition of Clostridium spp.

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

•Zinc is linked with the maintenance or function

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

•Stimulates the proliferation, differentiation and

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

ß-1,3 DIGESTIVE SUPPORT

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

1.5 kg/ton slow-release butyric acid

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

neither group showed diarrhoea

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

• 1,564 weaned piglets of 23 days of age (average 6.36 kg).

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

Legend: BG – Beta-1,3 glucan

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

• SR-Elite group (n=534)

1-14 days after weaning

Kemin internal refence:

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS PB6 – B. subtilis PB6 strain

Kemin internal refence:

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

PHILIPPINE SOCIETY OF ANIMAL NUTRITIONISTS

You might also like