Scribd Logo
Upload

Welcome to Scribd!

  • 62 views

    Enzyme Engineering

    Uploaded by

    Anukritti Mehra
    This document discusses enzyme engineering. Enzyme engineering involves designing enzymes by changing the amino acid sequence through recombinant DNA technology. The goal is to produce enzymes that are more useful for industrial applications by improving properties like kinetics, thermostability, pH tolerance and activity in organic solvents. There are two main methods for enzyme engineering - rational design and directed evolution. Rational design uses structural knowledge to make specific changes, while directed evolution mimics natural evolution by combining random mutagenesis with selection to evolve enzymes without structural information.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Enzyme Engineering

    Uploaded by

    Anukritti Mehra
    62 views13 pages
    This document discusses enzyme engineering. Enzyme engineering involves designing enzymes by changing the amino acid sequence through recombinant DNA technology. The goal is to produce enzymes that are more useful for industrial applications by improving properties like kinetics, thermostability, pH tolerance and activity in organic solvents. There are two main methods for enzyme engineering - rational design and directed evolution. Rational design uses structural knowledge to make specific changes, while directed evolution mimics natural evolution by combining random mutagenesis with selection to evolve enzymes without structural information.

    Original Description:

    Enzyme and Protein Engineering

    Original Title

    Enzyme engineering

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document discusses enzyme engineering. Enzyme engineering involves designing enzymes by changing the amino acid sequence through recombinant DNA technology. The goal is to produce enzymes that are more useful for industrial applications by improving properties like kinetics, thermostability, pH tolerance and activity in organic solvents. There are two main methods for enzyme engineering - rational design and directed evolution. Rational design uses structural knowledge to make specific changes, while directed evolution mimics natural evolution by combining random mutagenesis with selection to evolve enzymes without structural information.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    62 views13 pages

    Enzyme Engineering

    Uploaded by

    Anukritti Mehra
    This document discusses enzyme engineering. Enzyme engineering involves designing enzymes by changing the amino acid sequence through recombinant DNA technology. The goal is to produce enzymes that are more useful for industrial applications by improving properties like kinetics, thermostability, pH tolerance and activity in organic solvents. There are two main methods for enzyme engineering - rational design and directed evolution. Rational design uses structural knowledge to make specific changes, while directed evolution mimics natural evolution by combining random mutagenesis with selection to evolve enzymes without structural information.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 13

    ENZYME ENGINEERING

    PRESENTED BY:
    K.TAMILMUHILAN,
    I M.SC BIOTECHNOLOGY,
    ALAGAPPA UNIVERSITY.
    CONTENT

     Enzymes
     Enzyme Engineering
     Introduction
     Objective
     Steps Involved
     Types and methods
    ENZYMES

     Enzymes create chemical reaction in body.


     They actually speed up the rate of a chemical reaction to support life.
     The enzymes in your body perform very important tasks.
     Building muscles
     Destroying toxins
     Breaking down food particles during digestion
     An enzymes shape is tied to its function.
     Heat, disease or harsh chemical conditions can damage enzymes and change their shapes.
     When this happens, enzyme cannot work anymore.
    ENZYME ENGINEERING

     Enzyme engineering is the process of designing enzyme by changing the


    sequence of amino acids through recombinant DNA technology.
     These products will be useful as chemical , pharmaceutical, fuel, food or
    agricultural additives.
     Since enzymes are proteins, enzyme engineering is a part of the larger
    activity of protein engineering.
     Enzyme engineering utilizes r-DNA technology to introduce the desired
    changes in amino acid sequences of enzymes
    OBJECTIVES OF ENZYME ENGINEERING

     The chief objective of enzyme engineering is to produce an enzyme that is more useful for
    industrial and other applications.
     The various properties of an enzyme that may be modified to achieve this objective are as
    follows :-
     Improved kinetic properties.
     Elimination of allosteric regulation.
     Enhanced substrate and reaction specificity.
     Increased thermo stability.
     Alteration in optimal pH.
     Suitability for use in organic solvents.
     Increased/decreased optimal temperature.
    Steps involved in Enzyme Engineering
    STEPS INVOLVED:
    METHODS:

    There are two general strategies for


    enzyme engineering:
     Rational design
     Directed evolution
    RATIONAL DESIGN

     It is the earlier approach to enzyme engineering and still is widely used.


     In rational design, scientist uses detailed knowledge of structure and function of a protein to
    make desired changes.
     In general, it has a advantage of being inexpensive and technically easy.
     However, the major drawback is that a detailed structural knowledge of enzyme is often
    unavailable.
     Even when available, it can be very difficult to predict the effects of various mutations since
    structural information most often provide a static picture of enzyme structure.
     Two methods in rational design
     Overlap extension
     Whole plasmid single round PCR
    DIRECTED EVOLUTION:

     Also termed
     In vitro evolution
     Directed molecular evolution
     Acceleration evolution
     Directed evolution(DE) encompasses several molecular techniques that mimic the process
    of Darwinian evolution in vitro, by combining random mutagenesis or recombinant
    Technology.
     It does not uses structural data of enzyme, other than its functional properties and its
    corresponding DNA sequence.
     DE is sometimes referred to irrational or semi-rational design.
    Directed evolution

    Mimick recombination: DNA shuffling


    With restriction enzymes:
    1. Digest family of related genes
    2. DNA ligase to join fragments
    3. Chimeric genes
    THANK YOU😉

    You might also like