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    Chemistry 311

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    Charmaine Moyo
    The document summarizes an experiment to quantify the concentration of blue food dyes Brilliant Blue FCF and Indigo Carmine in Powerade Mountain Blast sports drink using a cellphone spectroscopic app. Measurements of the drink's absorbance at various concentrations were taken and calculations were done to determine the concentration of dyes in mg/L and μmol/L. The conclusion is that the results from the app were not entirely accurate as repeat measurements of the same drink sample gave different values.

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    Chemistry 311

    Uploaded by

    Charmaine Moyo
    24 views5 pages
    The document summarizes an experiment to quantify the concentration of blue food dyes Brilliant Blue FCF and Indigo Carmine in Powerade Mountain Blast sports drink using a cellphone spectroscopic app. Measurements of the drink's absorbance at various concentrations were taken and calculations were done to determine the concentration of dyes in mg/L and μmol/L. The conclusion is that the results from the app were not entirely accurate as repeat measurements of the same drink sample gave different values.

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    The document summarizes an experiment to quantify the concentration of blue food dyes Brilliant Blue FCF and Indigo Carmine in Powerade Mountain Blast sports drink using a cellphone spectroscopic app. Measurements of the drink's absorbance at various concentrations were taken and calculations were done to determine the concentration of dyes in mg/L and μmol/L. The conclusion is that the results from the app were not entirely accurate as repeat measurements of the same drink sample gave different values.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    24 views5 pages

    Chemistry 311

    Uploaded by

    Charmaine Moyo
    The document summarizes an experiment to quantify the concentration of blue food dyes Brilliant Blue FCF and Indigo Carmine in Powerade Mountain Blast sports drink using a cellphone spectroscopic app. Measurements of the drink's absorbance at various concentrations were taken and calculations were done to determine the concentration of dyes in mg/L and μmol/L. The conclusion is that the results from the app were not entirely accurate as repeat measurements of the same drink sample gave different values.

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    Cellphone Spectroscopic

    CHARMAINE MOYO
    32306938
    Chemistry
    Assignment
    13 March 2013
    Disodium 2-(4,5-dihydro-5-oxo-1-94-sulfophenyl0-4-
    (trifluoromethyl)-1H-pyrazol-3-yl) benzenesulfonate E133

    The Background story


    The blue color of Powerade Mountain Blast sports drink is caused by the addition of
    food coloring, particularly Brilliant Blue FCF and Indigo Carmine. These artificial,
    water-soluble dyes are frequently used to give food and drink items a vibrant blue
    color.

    As azo dyes, brilliant blue fcf and indigo carmine have nitrogen-nitrogen double
    bonds in their molecular composition. Due to the dyes' ability to capture light in the
    visible spectrum's orange to green region, these double bonds give the dyes their blue
    color.

    powerade
    Apparatus setup

    Table of relevant measurements

    Concentration/uM R value Concentration/uM absorbance


    0 161 0 0.026170234
    1 x10^5 140 1.68 0.086868075
    2 x10^5 120 3.36 0.153814864
    3 x10^5 108 5.04 0.199572355
    4 x10^5 90 6.72 0.278753601
    5 x10^5 85 8.4 0.303577185
    Table 1: Measurements captured

    Image 1: Graph of Conc. vs absorbance


    Calculations

    �1 = 8.4��� 68 is what was obtained from the app


    measuring the powerade only
    �1 = 20��
    �2 = �
    �2 = 100��
    �1 �1 = �2 �2
    8.4���×20��
    �2 =
    100��
    �2 = 1.68��� × 1000
    = 1680ppb × 0.001mg/L
    = 1.68mg/L ÷ 0.232019μmol/L
    = 7.2407999

    �1 = 8.4���
    �1 = 40��
    �2 = �
    �2 = 100��
    �1 �1 = �2 �2
    8.4��� × 40��
    �2 =
    100��
    �2 = 3.36���
    = 3360ppb
    = 3.36mg/L
    = 14.48157263μmol/L

    �1 = 8.4���
    �1 = 60��
    �2 = �
    �2 = 100��
    �1 �1 = �2 �2
    8.4��� × 60��
    �2 =
    100��
    �2 = 5.04���
    = 5040ppb
    = 5.04mg/L
    = 21.72235894μmol/L

    �1 = 8.4���
    �1 = 80��
    �2 = �
    �2 = 100��
    �1 �1 = �2 �2
    8.4��� × 80��
    �2 =
    100��
    �2 = 6.72���
    = 6720ppb
    = 6.72mg/L
    = 28.96314526μmol/L

    �1 = 8.4���
    �1 = 100��
    �2 = �
    �2 = 100��
    �1 �1 = �2 �2
    8.4��� × 100��
    �2 =
    100��
    �2 = 8.4���
    = 8400ppb
    = 8.4mg/L
    = 36.20393157μmol/L

    Conclusion
    Based on the results that was retrieved, the results are not entirely accurate because
    when measuring the pigment with the app many times it gave out different values.
    Which means the App was in the wrong this time because the water was kept constant
    and even the measurements were not changed in some way.

    References

    Rodman, D.C. and Jarlskog, C., 2018. Spectroscopy and RGB-Colorimetry for
    Quantification of Plant Pigment and Fruit Content in Fruit Drinks (Doctoral
    dissertation, BS thesis, Lund University, Lund, Sweden).

    Puchalska, M., Połeć‐Pawlak, K., Zadrożna, I., Hryszko, H. and Jarosz, M., 2004.
    Identification of indigoid dyes in natural organic pigments used in historical art
    objects by high‐performance liquid chromatography coupled to electrospray
    ionization mass spectrometry. Journal of mass spectrometry, 39(12), pp.1441-1449.

    Pérez-Arantegui, J., Resano, M., García-Ruiz, E., Vanhaecke, F., Roldán, C., Ferrero,
    J. and Coll, J., 2008. Characterization of cobalt pigments found in traditional
    Valencian ceramics by means of laser ablation-inductively coupled plasma mass
    spectrometry and portable X-ray fluorescence spectrometry. Talanta, 74(5), pp.1271-
    1280.

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