Manual on meat inspection for developing countries

CHAPTER 3
SPECIFIC DISEASES OF CATTLE
Diseases caused by viruses
Foot and mouth disease (FMD, Aphthous fever)
FMD is an acute viral and extremely contagious disease of cloven footed animals
such as cattle, sheep, goats, pigs and antelope. It is manifested by vesicles and
erosions in the muzzle, nares, mouth, feet, teats, udder and pillar of the rumen.
There are three main strains of viruses causing FMD, namely A, O and C. Three
additional strains, SAT 1, SAT 2 and SAT 3 have been isolated from Africa and a
further strain ASIA-1 from Asia and the Far East.

Transmission: Direct and indirect contact with infected animals and their
secretions including saliva, blood, urine, faeces, milk and semen, aerosol droplet
dispersion, infected animal by-products, swill containing scraps of meat or other
animal tissue and fomites and vaccines.

Antemortem findings:

Before vesicle formation:

Incubation is 1 - 5 days or longer

Morbidity: Nearly 100 %
Mortality: variable depending on the strain of virus and its virulence and
susceptibility of host; 50 % in young animals, 5 % in adults
Fever up to 41.7°C
Dullness
Lack of appetite
Drastic drop in milk production.
Uneasiness and muscle tremors
Vesicle formation:

Smacking and quivering of lips

Extensive salivation (Fig. 45) and drooling
Shaking of feet and lameness
The vesicles and later erosions are commonly found on the muzzle, tongue (Fig. 46),
oral cavity, teat and on the skin between and above the hoofs of the feet. In more
chronic cases in cattle the hoof become loose and the animal may walk with
characteristic “clicking” sound (Slippering).

Some strains of FMD, particularly in swine, sheep and goats cause erosions instead
of vesicles.

Postmortem findings :

Necrosis of heart muscle(tiger heart), usually only in young acutely infected

animals.

Ulcerative lesions on tongue, palate, gums, pillars of the rumen and feet.

Judgement : In countries or in zones within a country free or nearly free of FMD

diseased or suspect animals are prohibited to be admitted in an abattoir or
slaughtered. If FMD is suspected on postmortem examination the carcass and viscera
are condemned and appropriate action recommended by the regulatory authorities of
the country must be taken. In countries where this disease is present, the
judgement should be in accordance with the current animal health requirements, and
consisted with effective public health protection. Particular attention should be
paid to secondary bacterial infections and general findings. Sanitary measures
should be taken to comply with national animal health policy.
Remarks : Latent infections with Salmonella organisms were reported in animals
affected with FMD.

Differential diagnosis in bovine and ovine species : Vesicular stomatitis, allergic

stomatitis, feedlot glossitis, photosensitization, bluetongue, rinderpest,
infectious bovine rhinotracheitis, malignant catarrhal fever, bovine papular
stomatitis, bovine viral diarrhoea, pseudocowpox, ovine pox, contagious ecthyma,
footrot, mycotoxicosis and increased salt in concentrate.

Discussion : In order to prevent the spread of the virus in the abattoir, the
equipment and room should be disinfected with 2 % NaOH (caustic soda). In some
countries sodium carbonate (Na2CO3) is used. The vehicle conveying diseased animals
should also be disinfected and abattoir personnel leaving the abattoir should pass
through a footbath with 1 % solution of NaOH.

The virus of FMD can survive in meat and meat products for a considerable length of
time. Outside the pH range of 6 – 9, viral infectivity is destroyed. A bovine
carcass matured at above +2°C produces a drop in the pH of muscle tissue to between
5.3 – 5.7 within 24 hours of slaughter. This is caused by the formation of
sarcolactic acid. Quick freezing of the meat arrests acid production and
consequently the virus remains infective for about 6 months. In salted meat at 4°C,
the virus is still infective in bone marrow and lymph nodes for 6 months. In blood
clots in large vessels of cattle and swine, the virus is infective for 2 months.
The virus is inactivated by ultraviolet rays, acetic acid, 2 % lye and ethylene
oxide. At high temperatures, the virus is only active for a short period. 2 % NaOH
solution inactivates the virus in 1 – 2 minutes. In dry refuse in stalls, the virus
remains infective for 14 days, 3 days on soil surfaces in summer compared to 39
days in fall. It is also infective for 39 days in urine and for 20 weeks on hay
dried at 22°C. The virus can be destroyed with 0.5 % citric or lactic acid, by
cooking meat to an internal temperature of 69°C and by pasteurization processes of
milk.

Fig. 45: Excessive salivation in a cow affected with FMD.

Fig 46: FMD. Extensive areas of eroded epithelium on a bovine tongue.

Rinderpest (RP)
Rinderpest is an acute, highly contagious, fatal viral disease of cattle, buffalo
and wild ruminants manifested by inflammation, haemorrhage, erosions of the
digestive tract, wasting and often bloody diarrhoea. Some swine species are also
susceptible. Man is not susceptible to RP virus.

Transmission : Direct contact with infected animals or their excretions and

secretions and fomites. The virus appears in the blood and in secretions before the
onset of clinical signs and this may cause infection in abattoirs and stockyards.

Antemortem findings :

Incubation: 3 – 10 days or longer

Morbidity: Up to 100 % in a susceptible herd
Mortality: 50 % and may reach 90 – 95 %
High fever (41–42°C)
Nasal discharge and excessive salivation
Punched out erosions in the mouth (Fig. 47)
Loss of appetite and depression
Abdominal pain (grunting, arched back)
Constipation followed by bloody diarrhoea and straining
Dehydration and rough hair coat
Marked debility
Abortion
The classical “milk fever position” in cattle
Postmortem findings :

Punched out erosions in the oesophagus

Edema or emphysema of the lungs
Haemorrhage in the spleen, gallbladder and urinary bladder
Haemorrhagic or ulcerative lesions in the omasum
Congested abomasum filled with bloody fluid. Ulcers may also be observed.
Severe congestion and haemorrhage in the intestine and enlarged and necrotic
Peyer's patches (Fig. 48)
Last portion of the large intestine and rectum are haemorrhagic showing “tiger
stripping” of longitudinal folds
Enlarged and edematous lymph nodes
Emaciated carcass
Judgement : The carcass derived from a feverish and debilitated animal showing the
sign of acute disease on antemortem examination should be condemned. In the areas
free of RP and in zones where final stages of eradication exist, the animals are
also condemned. In endemic zones, if acute symptoms of the disease are not present
during clinical examination, the carcass may have limited distribution. In areas
affected with outbreak which are protected by vaccination, heat treatment of meat
is suggested if economically worthwhile. The affected organs are condemned.

Remarks : Rinderpest virus is sensitive to environmental changes and is destroyed

by heat, drying and great number of disinfectants.

Differential diagnosis : Bovine viral diarrhoea, malignant catarrhal fever,

infectious bovine rhinotracheitis, bluetongue, coccidiosis, foot and mouth disease,
vesicular and necrotic stomatitis and bovine papular stomatitis. Vesicular diseases
do not have accompanying haemorrhage and blisters should be differentiated from
erosions (ulcers) seen at RP.

Fig. 47: Rinderpest Erosions on the dental pad and the hard palate which resemble
FMD.

Fig. 48: The mucosal surface of Peyer's Patches showing necrosis and congestion.

Vesicular stomatitis (VS)

This is a viral disease of ruminants, horses and swine characterized by vesicular
lesions of the mouth, feet and teats. VS virus has two immunologically distinct
serotypes, Indiana and New Jersey.

Transmission : In susceptible animals, contamination of pre-existing abrasions with

saliva or lesion material, by ingestion of contaminated pasture or during milking
within dairy herds. Mechanical transmission by biting arthropods is also a
possibility. The virus is isolated from mites, tropical sand flies and mosquitos.

Antemortem findings :

Fever
Mouth lesions in cattle and horses
Vesicles tend to disappear quickly and only papules may be seen in cattle
outbreaks.
Marked weight loss and cessation of lactation in dairy cows.
Chewing movements and profuse salivation
Refuse food but eagerly accept water
Horses rub lips on edges of mangers
Foot lesions occur in about 50 % cases in cattle.
Lameness
Teat lesion may occur in all species.
Postmortem findings :

The skin and mucous membrane lesions resemble the lesions of other vesicular
diseases.
Secondary bacterial or fungal infections
Mastitis
Judgement : The carcass of an animal affected with vesicular stomatitis is approved
if the disease is not in the acute stage and secondary changes are not present.
Parts of the affected carcass and organs are condemned. A carcass showing acute
changes and systemic lesions is condemned. If VS is not confirmed by laboratory
examination, the judgement will be the same as for the FMD.

Differential diagnosis : Foot and mouth disease, swine vesicular exanthema,

vesicular disease, bovine papular stomatitis

The mouth and muzzle lesions: Bovine viral diarrhoea, rinderpest, mycotic
stomatitis, photosensitization and Potomac valley fever in horses

Teat lesions: Cowpox, pseudo-cowpox, pseudo-lumpy skin disease and bovine herpes
mammillitis

Fig. 49: Vesicular stomatitis. Tongue lesions.

Malignant catarrhal fever (MCF)

An acute viral disease of cattle, deer, bison and buffalo characterized by
inflammation of mucous membranes of the nose, eyes, corneal opacity, profuse nasal
discharge and enlargement of lymph nodes. MCF is arbitrarily divided into peracute,
intestinal, head-eye and mild forms according to antemortem findings. It is not
communicable to man.

Transmission: Close contact between cattle and wildebeest (gnu, antelope), by

common use of drinking troughs or by direct contact between cattle and newborn
wildebeest and placenta of parturient dams. In American or European MCF, cattle are
infected from sheep.

Antemortem findings :

Incubation: 9 – 44 days
Morbidity is low and mortality is high
Increased temperature
Bilateral ocular and nasal discharges
Dyspnea and cyanosis
Loss of appetite
Encrustation of muzzle and eczema of the perineum, scrotum and udder
Erosions on the lips, tongue, gums, soft and hard palate
Swollen reddened eyelids, corneal opacity and conjunctivitis (Fig. 50)
Photophobia associated with corneal opacity and blindness
Reluctance to swallow because of oesophageal erosions and drooling
Enlarged body lymph nodes
Rarely, uncoordinated movements and shivering
Postmortem findings :
Lesions are not present in acute cases
Crater like erosions of the nose, mouth, conjunctiva, oesophagus and
gastrointestinal tract
Lungs may be congested, swollen or emphysematous
White areas in the kidneys
Swollen and reddened abomasal folds
Intestinal edema and petechial haemorrhage
“Tiger striping” in the distal colon (Fig. 51)
Enlarged and reddened lymph nodes
Dehydrated and emaciated carcass
Judgement :In the early stages of the disease, when fever, emaciation and systemic
signs are lacking, the carcass of the affected animal may be approved as inferior
meat. Otherwise, when fever, emaciation and systemic signs are present, the entire
carcass and viscera are condemned. The condemned material may be used for
rendering.

Differential diagnosis : Bluetongue, rinderpest, bovine viral diarrhoea/mucosal

disease, foot and mouth disease, vesicular stomatitis

Fig. 50: Malignant catarrhal fever Early stages of corneal opacity, conjunctivitis
and the reddening of the eye lids.

Fig. 51: Malignant catarrhal fever. “Tiger striping” in the distal colon.

Rift valley fever (RVF) (see Chapter 5)

Rabies
This is an acute infectious viral disease of the central nervous system in mammals.

Transmission : It is usually transmitted through the saliva by a bite from a rabid

animal, commonly the dog or jackal. Man is infected the same way.

Antemortem findings :

Furious form

Incubation from 2 weeks to 6 months or longer

Restlessness
Aggressive, may attack other animals
Sexual excitement
Bellowing
Sagging and swaying of the hind quarters
Drooling and salivation
The tail is held to one side
Tenesmus or paralysis of the anus
Paralysis
The animal falls to the ground
Death after 48 hours of decubitus
Postmortem findings: Possible inflammation of gastrointestinal mucosa

Judgement: In endemic areas carcasses may be approved if the animal was bitten
eight days before slaughter and within 48 hours of slaughter. The bite area and
surrounding tissue must be condemned, and prevention taken to prevent occupational
hazards.

Differential diagnosis : Indigestion, milk fever or acetonemia when first seen,

foreign body in the mouth, early infectious disease, poisoning
Discussion: In a diseased animal, the virus is found in saliva, salivary gland and
nervous tissue. Extreme caution should be instituted in abattoirs in order to
prevent occupational hazards. Abattoir personnel can contract the disease through
surface contact with infected tissue. Infection does not occur by consumption of
meat from a rabid animal.

Slaughter may be prohibited during a quarantine period of 8 months following

exposure to the disease. An animal suspected of having rabies should be placed
under a “Held tag”. The warning sign should read “The animal is not to be handled”.
Any person who was in touch with the animal should thoroughly wash his/her hands
with strong soap and/or disinfectant. If possible, the wound should be opened to
encourage bleeding in order to flush out the virus and expose the deeper area of
the wound. Tincture of iodine (up to 0.001 % aqueous solution of iodine or ethanol
43.70%) should be further applied.

Lumpy skin disease

Acute pox viral disease of cattle manifested with sudden appearance of nodules on
the skin.

Transmission : Insect vectors by direct and indirect transmission. Seasonal and

geographic distribution.

Antemortem findings :

Incubation: 4 – 14 days
Fluctuating fever
Diarrhoea
Nasal discharge and salivation
The first lesion appear in the perineum
Various sized cutaneous nodules (Fig. 52) may occur throughout the body
Skin lesions may show scab formation
Swelling of superficial lymph nodes and limbs, and lameness
Infertility and abortion
Secondary infection may lead to joint and tendon inflammation
Postmortem findings :

Ulcerative lesions in the mucosa of the respiratory and digestive tract

Reddish, haemorrhagic to whitish lesions in the lungs
Edema (interlobular) and nodules in the lungs (Fig. 53)
Heart lesion (endocardium)
Thrombosis of skin vessels followed by cutaneous infarction and sloughing.
Judgement : Carcass of an animal showing mild cutaneous lesions and no fever
associated with general signs of infection is conditionally approved pending heat
treatment. The affected parts of the carcass and organs are condemned, Carcass of
an animal showing, on antemortem examination, generalized acute infection
accompanied with fever, is condemned.

Differential diagnosis : Allergies, screw-worm myiasis, urticaria, dermatophilosis

(streptothricosis), bovine herpes dermophatic infection, cattle grubs, vesicular
disease, bovine ephemeral fever, photosensitization, besnoitiosis (elephant skin
disease), sweating weakness of calves, bovine farcy and skin form of sporadic
bovine lymphomatosis

Fig. 52: Lumpy skin disease. Various sized cutancous nodules in a severe case of
lumpy skin disease.

Fig. 53: Cut surface of the nodules in the parenchyma of the lung and interlobular
edema.

Bovine herpes dermophatic disease (BHD)

A herpes virus infection of cattle and sometimes sheep and goats manifested by
cutaneous lesions and fever.

Transmission : Biting insects, mechanical milking

Antemortem and postmortem findings :

Incubation: 3–7 days

Morbidity: High in primary infections
Fever
Cutaneous nodules. At first these are round, then later become flattened and
covered with dry scabs (Fig. 54).
Hairless skin is normal after the scab falls off.
Ulcerative lesions of the teats and udder (Fig. 55)
Erosions between the digits
Microscopy reveals intranuclear inclusions and giant cells in the skin.

Judgement : Carcass of an animal affected with BHD is disposed similar to an animal

affected with lumpy skin disease

Differential diagnosis : Dermatophilis infection, cowpox and pseudocowpox,

vesicular stomatitis and lumpy skin disease. The latter is differentiated from BHD
by enlarged lymph nodes.

Fig. 54: Bovine herps dermophatic disease. Dried scabs on the skin of the neck.

Fig. 55: BHD.

Ulcerative lesions of the teats and udder.

Infectious bovine rhinotracheitis (IBR)

IBR is a highly infectious viral respiratory disease of cattle, goats and pigs
manifested by inflammation of respiratory passages and pustular lesions on the male
and female genital organs. Generally four forms of the disease are recognized; the
respiratory form, the genital form, the enteric form and the encephalitic form.

Transmission: Respiratory droplet and nasal exudate in the respiratory form of IBR.
Obstetrical operations, coitus and licking of genitalia of affected animals in the
genital form of disease.

Antemortem findings:

Respiratory form

Incubation: 5 – 14 days
Fever
Nasal and ocular discharge and red, swollen conjunctiva
Drop in milk yield
Breathing through the mouth and salivation (Fig. 56)
Hyperaemia of the nasal mucosa and necrotic areas on the nasal septum
Secondary bronchopneumonia
Abortion
Genital form
Frequent urination and tail elevation
Edematous swelling of the vulva and pustule formation on reddened vaginal mucosa
Mucoid or mucopurulent exudate in the vagina
Enteric form

Severe oral and stomach necrosis in new born animals

High mortality
The encephalitic form in calves

Depression
Excitement
High mortality
Postmortem findings:

Acute inflammation of the larynx, trachea (Fig. 57) and bronchi

Profuse fibrino-purulent exudate in the upper respiratory tract in severe cases
Chronic ulcerative gastro-enteritis in feedlot cattle
Lung emphysema
Secondary bronchopneumonia
Judgement: Carcass of an animal affected with IBR is approved if signs of acute
infection are not present and the animal is in good body condition.

Differential diagnosis: Pneumonic pasteurellosis, bovine viral diarrhoea, malignant

catarrhal fever and calf diphtheria

Fig. 56: Breathing through the mouth and salivation in a bovine affected with IBR.

Fig. 57: IBR. Acute inflammation of the larynx and trachea

Bovine viral diarrhoea (BVD)

This is an infectious viral disease of cattle manifested by an active erosive
stomatitis, gastroenteritis and diarrhoea.

Transmission: Direct contact with clinically sick or carrier animals, indirect

contact with feedstuffs or fomites contaminated with urine, nasal and oral
secretions or faeces and contact with aborted fetuses. Transmission through aerosol
droplet dispersion or by insect vector may also be a possibility. Virus may persist
in recovered and chronically ill cattle which are considered a potential source of
infection.

Antemortem findings :

Incubation: 1 – 3 days
Fever
Congestion and erosions in the mucous membranes of the oral cavity
Depression and anorexia
Cough, polypnea and salivation
Dehydration and debilitation
Foul-smelling diarrhoea
Cessation of rumination
Reduced milk supply
Abortion in pregnant cows
Laminitis
Congenital anomalies of the brain (cerebellar ataxia) and arthritis in young calves
Postmortem findings :

Shallow erosions present on the entrance of the nostrils, mouth, pharynx, larynx,
oesophagus, rumen (Fig. 58), omasum, abomasum (Fig. 59), caecum and less frequently
in Peyer's patches in the small intestine.

Erythema of the mucosa with submucosal haemorrhage in the abomasum, small

intestine, caecum and colon. Stripped appearance on the caecal and colon mucosa is
similar to that seen in rinderpest.

Cerebral hypoplasia and cataracts in calves.

Judgement: Carcass and viscera of an animal, which on antemortem examination showed

generalized signs of acute infection accompanied with fever and/or emaciation, are
condemned. Chronic cases of BVD with no systemic involvement have a favourable
judgement of carcass, viscera and organs.

Differential diagnosis : Malignant catarrhal fever, rinderpest, blue tongue and

vesicular diseases. The latter produce vesicles which are not present in BVD.
Diseases with no oral lesion nor diarrhoea include salmonellosis, Johne's disease
and parasitism.

Fig. 58: BVD. Congestion and erosions in the ruminal mucosa.

Fig. 59: BVD. Inflammation of the abomasum (abomasitis, gastritis).

Bovine leukosis
Bovine leukosis is a persistent and malignant viral disease of the lymphoreticular
system. It occurs in all breeds and in both sexes.

Bovine leukosis is observed in two forms : a) the sporadic and b) the enzootic
form. The sporadic form is rare and occurs in cattle under three years of age. The
enzootic form is most commonly found in adult cattle, particularly in cull cows.

Transmission: By small amounts of infected blood (e.g. infected needles,

dehorning), vertical transmission from the dam to the calf (3 – 20 % of calves may
become infected) and by colostrum or milk (less than 2 %). Insect transmission is
also a possibility; higher rates of infection were reported in the summer.

Antemortem findings :

Laboured breathing due to heart involvement

Persistent diarrhoea following infiltration of the abomasum wall by neoplastic
cells
Marked enlargement of several superficial lymph nodes
Edema of the brisket and the intermandibular region
Paralysis of the hind legs due to tumour compression of the spinal cord
Protrusion of the eye as a result of tumour invasion of the orbital cavity
Debilitation or emaciation
Pale mucosal surface
Bloated animal
Swelling of the neck when thymus is involved
Cutaneous nodules in the terminal stage
Postmortem findings:

Lymph node enlargement (clay-like consistency)

Enlargement of spleen (splenomegaly)
Thin watery blood
Neoplastic lesions in the heart (Fig. 60), intestines (Fig. 61) (Virtually all of
the organs may be involved.)
Ventral edema
Enlarged haemolymph nodes
Judgement: Carcass of an animal affected with leukosis (lymphosarcoma) is
condemned. When a diagnosis cannot be made by postmortem findings, a laboratory
diagnosis should be performed. If lymph node hyperplasia is the histological
diagnosis, the carcass is approved for human consumption. Depending on disease
prevalence, leukosis reactors may be totally approved or conditionally approved
pending heat treatment.

Differential diagnosis : Lymphadenitis, lymphoid hyperplasia, hyperplastic

haemolymph nodes, pericarditis, enlarged spleen in septicemic conditions, other
neoplasms and parasitism.

Fig. 60: Leukosis. Neoplastic mass infiltrating the heart muscle.

Fig. 61: Leukosis. Neoplastic growths in the intestine. Both lesions were
histologically confirmed as lymphosarcoma.

Bovine spongiform encephalopathy (BSE, “Mad cow disease”)

BSE is a progressive and fatal disease of adult cattle characterized by a
progressive degeneration of the central nervous system causing neurological signs
in animals. Some scientists suspect that an unusual and atypical virus-like
transmissible agent called a prion is associated with the etiology of BSE. Prion is
the term currently used in literature.

Transmission : The ingestion of protein feed supplements prepared from sheep meat
or sheep by products contaminated with scrapie virus.

Antemortem findings :

Incubation period 2 – 8 years

Reduction in milk production
Weight loss, while maintaining good appetite
Behaviour changes (nervousness and aggressiveness), kicking in the milking parlour
The progressive degeneration of the central nervous system causes neurologic signs:

Apprehension, teeth grinding

Tremors and abnormal ear position
Abnormal posture and disorientation
Incoordination and stiff gait
Paresis
Recumbency and death

Fig. 62: BSE. Degenerative lesion in the cerebral cortex.

Diagnosis can be confirmed only on the postmortem histological examination of brain

tissue. Microscopic lesions include degenerative lesions of the cerebral cortex
(Fig. 62), medulla and central grey matter of the midbrain.

Judgement : Carcass is condemned.

Differential diagnosis : Rabies, listeriosis, bovine pseudorabies (mad itch), other

brain infections in cattle, the nervous type of acetonemia, hypocalcemia,
hypophosphatemia and hypomagnesemic tetany

Discussion : The first reported cases of this disease were in dairy cows in 1987
from different locations in the United Kingdom. The disease is now also recognized
in some other countries in and outside Europe. BSE belongs to a group of human and
animal diseases classified as transmissible spongiform encephalopathies.
Significant human diseases of this group are Kuru and Creutzfeldt-Jacob's disease.
Scrapie, which affects sheep and goats also belongs in this group.

Researchers are trying establish if BSE and scrapie have the same causative agent,
and if the modified form of the scrapie agent is also a possible causative agent of
BSE. Prions are also the causative agents of transmissible mink encephalopathy
(TME) and of chronic wasting disease (CWD) of mule deer and elk.

BSE affects only adult animals and the incidence within-herd is low. The breed,
gender or year and seasons are not associated with the development of this disease,
nor is contact with sheep. In order to control this disease, in the U.K. the
following actions were taken:

Ruminant derived protein is prohibited in all ruminant rations.

The consumption of milk from affected animals by humans or animals is also
prohibited.
Bovine brain cannot be used for human consumption.
The mandatory slaughter of all animals manifesting signs of BSE and compensation
awarded to the owner.
Diseases caused by Rickettsia and Mycoplasma spp.
Heartwater (Hydropericardium)
“Black dung” when affecting African cattle and buffalo

“Sheep fever” when seen in sheep

Heartwater is an acute, non contagious disease of cattle, sheep, goats, antelopes

and wild ruminants. It is caused by the rickettsial organism Cowdria (Rickettsia)
ruminantium.

Transmission: Heartwater is transmitted by various species of Amblyomma ticks.

Transstadial transmission of the organism occur in vector ticks.

Antemortem findings :

Peracute form

Nervous signs include

Twitching of the eyelids

Protrusion of the tongue
Champing of the jaw
Walking in circles
Paddling with legs in recumbent animals
Opisthotonos and convulsions
Postmortem findings :

Hydropericardium
Hydrothorax
Pulmonary edema and ascites
Haemorrhagic gastroenteritis
Enlarged liver, spleen and lymph nodes
Haemorrhage in the abomasum and intestine
Edema and haemorrhage of the brain
Judgement : Carcass of an animal affected with heartwater is condemned in the acute
stage of the disease. In a chronic case, the carcass may be approved if adequately
bled and muscles are wholesome in colour and texture. The affected organs are
condemned.
Differential diagnosis : Peracute form of heartwater should be differentiated from
anthrax. The acute nervous form of the disease is differentiated from tetanus,
rabies, cerebral trypanosomiasis, strychnine poisoning, piroplasmosis,
theileriosis, lead and organophosphate poisoning, parasitism, arsenical poisoning
and poisoning with certain plants.

Fig. 63 : Heartwater Cowdria ruminantium in bovine brain smear (arrow).

Q fever (Queensland fever, Nine mile fever, American Q fever, Australian Q fever)
Q fever is a disease of cattle, sheep, goats, donkeys, camels, fowl, dogs, cats,
pigeons and humans. It is caused by Coxiella burnetii. Q fever is an occupational
disease of livestock personnel. farmers and laboratory personnel.

Transmission : Ticks spread infection to cattle which develop mild disease. The
faeces deposited on animal hide by ticks may be the source of infection for humans.
Q fever is also transmitted by inhalation or dust contaminated with infected animal
secreta or excreta. Healthy animals may serve as a carrier and shed the organism in
milk, urine, faeces, placenta and fetal fluids. They harbour the infection and no
clinical signs are observed. Contaminated meat and water are further means of
infection read.

field cases there are no clinical signs of this disease. In the disease produced by
the inoculation of cows via the udder the clinical signs may include:

Acute mastitis
Loss of appetite and depression
Serous nasal and lacrimal discharge
Difficult breathing
Atony of the rumen
Abortion in pregnant cows
No gross lesions are reported in cattle.

Discussions : Coxiella burnetii is highly resistant and was isolated from farm soil
6 months after the removal of animals. It may persist in the udder up to 3 years.
The temperatures of milk pasteurisation (in bulk at 63°C for 30 minutes or the
common method at 72°C for 15 seconds) kill this agent in milk. Vaccination will
reduce shedding of organisms in milk.

This disease in humans has a sudden onset and is characterized by loss of appetite,
weakness and generalized malaise lasting from 1 – 2 weeks. Pneumonia may also be
present. Death may be caused by endocarditis in older people. More severe symptoms
of Q fever are noticed.

Contagious bovine pleuropneumonia

This is an acute, subacute or chronic highly infectious disease of cattle caused by
Mycoplasma mycoides var, mycoides.

Transmission : Aerosol and droplet infection from the infected animals. The
recovered animal called “lungers” act as carriers and shedders, especially under
stress.

Antemortem findings :

Incubation: acute 10 – 14 days, chronic 3 – 6 months

Morbidity: 90 % in susceptible cattle
Mortality: 10 – 50 %
Fever
Depression
Lack of appetite and loss of weight
Coughing on exercise
Shallow rapid respiration, grunting and gurgling
Extended neck, lowered head and open mouth
Arched back and outward rotated elbow
Arthritis in young animals
Postmortem findings :

Fibrinous inflammation of the pleura (pleuritis)

Straw coloured fluid in the thorax (Fig. 64)
Lobar pneumonia with red hepatization, marbled appearance of lung lobules (Fig. 65)
due to thickening of interlobular septae and interlobular pulmonary edema
Enlarged mediastinal lymph nodes
Walled-off sequestra formation in chronic cases
Haemorrhage in the heart
Arthritis and tenosynovitis
Judgement : Carcass of an animal affected with contagious bovine pleuropneumonia is
condemned if the disease is associated with fever, inadequate bleeding of carcass,
serous infiltration of the brisket and emaciation. Recovered animals showing no
generalized signs of the disease are approved and the affected organs are
condemned.

Differential diagnosis : Shipping fever (Pasteurellosis). East coast fever, foreign

body pneumonia, IBR, tuberculosis, chlamidial infections and lungworms

Fig. 64: Contagious bovine pleuropneumonia. Straw coloured fluid in the thorax and
partial lung hepatization.

Fig. 65: Contagious bovine pleuropneumonia. Lobar pneumonia with red hepatization
and marbled appearnce of lung lobules.

Diseases caused by bacteria

Black quarter (Black leg)
Black quarter is an acute infectious disease of cattle and sheep manifested by
severe inflammation of the muscle with high mortality. It is caused by Clostridium
chauvoei.

Transmission : The organisms of blackleg are found in the soil. During grazing,
organisms may enter the digestive tract of a susceptible animal. Clostridium
chauvoei is also found in the digestive tract of healthy animals. In sheep the
agent is transmitted through wounds at shearing, docking and castration and during
lambing in ewes.

Antemortem findings :

High fever (41°C)

Lameness
Loss of appetite
Discoloured, dry or cracked skin
Stiff gait and reluctance to move
Crepitating swellings often on the hips and shoulder
In sheep gaseous crepitation cannot be felt before death
Postmortem findings :

Laying on one side with affected hind leg stuck out. Commonly seen in cattle
Bloating of carcass and blood stained frothy exudates from the nostrils and anus
Dark red to black muscle of the loin, back or leg (Fig. 66)
Spongelike bubbly appearance of the muscles with a peculiar rancid odour
Yellowish, gelatinous subcutaneous tissue and associated gas bubbles
Blood stained fluid in body cavities

Fig. 66: Black leg. Dark-red skeletal muscle of a heifer showing haemorrhage,
necrosis, edema and emphysema.

Judgement : Carcasses of animals affected with black leg should be condemned. It is

prohibited to slaughter and dress an animal diagnosed with this disease at
antemortem examination.

Differential diagnosis : Other acute Clostridial infections, lightning strike,

anthrax, bacillary haemoglobinuria, lactation tetany, extensive haemorrhage and
acute lead poisoning.

Discussion : Black leg is worldwide in distribution. Well nourished animals are

more frequently affected. It is also more commonly seen in grass fed animals than
in stall fed animals. Clostridia are soil-borne organisms which cause disease by
releasing toxins. Specific antitoxin and antibiotics are rarely effective in the
treatment of this disease. An adequate preventive vaccination program may be the
most effective method in protecting the animals from black leg.

Botulism
Botulism is a disease manifested by progressive muscular paralysis. It is seen in
humans, animals, birds and fish and is caused by various strains of Clostridium
botulinum.

Transmission : Decomposed flesh and bones are the source of infection for animals.
Incubation period 12 – 24 hours. However, 2 hours up to 14 days incubation period
has been recorded.

Antemortem findings :

In cattle and horses

Restlessness
Knuckling and incoordination
Paralysed tongue and drooling of saliva
Sternal recumbency
Progressive muscular paralysis from hindquarters to frontquarters, head and neck
(Fig. 67)
In sheep

Serous nasal discharge and salivation

Abdominal respiration
Stiffness upon walking and incoordination
Switching of the tail on the side
Limb paralysis and death
In pigs

Lack of appetite, refusal to drink and vomiting

Pupillary dilatation
Muscular paralysis
Postmortem findings : Foreign material in fore-stomachs or stomachs may be
suggestive of botulism.

Judgement : Total condemnation of carcass because of human hazards.

Differential diagnosis : Parturient paresis, paralytic rabies, equine
encephalomyelitis, ragwort poisoning in horses, miscellaneous plant poisoning.

In sheep - louping ill, hypocalcemia and some cases of scrapie.

Discussion : Cl. botulinum is found in the digestive tract of herbivores. Soil and
water contamination occurs from faeces and decomposing carcasses. The proliferation
of Cl. botulinum organisms may also occur in decaying vegetable material. Sporadic
outbreaks of botulism are reported in most countries. Outbreaks of botulism in
cattle and sheep in Australia, Southern Africa and the Gulf coast area of the
United States are associated with phosphorus deficient diets and ingestion of
carrion. Cattle, sheep and rarely swine are susceptible to this disease. Dogs and
cats are resistant.

Cl. botulinum produces neurotoxin which causes functional paralysis. Seven strains
of this organism (“ A through G ”) are distinguished according to immunological
differences. The diseases caused by various strains of this agent are frequently
regarded as a separate entity owing to some of their prominent signs. Names such as
“Bulbar paralysis in cattle”, “Lamsiekte in sheep” in South Africa (meaning lame
sickness), and “Limberneck in poultry” are often used. Cl. botulinum is often found
in anaerobic conditions of deep wounds. It produces neuroparalytic exotoxins which
cause symptoms of the disease. This organism will grow and produce toxins if the
temperature is between 10 – 50°C, pH above 4.6. water activity (AW) above 0.93 and
anaerobic conditions exist. Fresh meats are implicated with less than 10 % of
botulism outbreaks. The major sources of this organism are fish, home cured meats,
home canned vegetables and fruit. Eggs, milk and their products are rarely the
cause of an outbreak. Most frequently, raw, insufficiently cooked foods or foods
not fully salted, cured, dried or smoked are implicated. Botulism toxins are heat
labile and food suspected of having the organism should be boiled before serving.

In man the signs of the disease are weakness, dizziness, blurred or double vision,
dilatation of pupils, dry mouth, difficulties in breathing and speech, progressive
muscular weakness, respiratory failure and death. Pneumonia may be a complication
associated with botulism in man.

Fig. 67: Botulism. Sternal recumbency. Muscular paralysis of hind and front
quarters.

Malignant edema
Malignant edema is a bacterial disease of cattle, sheep, goats, swine, horses and
poultry. It is caused by Clostridium septicum and is manifested by wound infection.
The infection is commonly soil-borne. Deep wounds associated with trauma provide
ideal condition for the growth of this agent.

Antemortem findings :

Postmortem findings:

Gangrene of the skin in area of infection site

Foul putrid odour is frequently present
Gelatinous exudate in the subcutaneous and intramuscular connective tissue
Subserosal haemorrhage
Accumulation of sero-sanguineous fluid in body cavities
Muscle tissue is dark-red but has little or no gas
Judgement : Carcasses of animals affected with malignant edema are condemned.

Differential diagnosis: Blackleg. In malignant edema the muscle is not involved and
the wound site is noted. Anthrax in pigs. Subcutaneous edema in the throat region
is present.

Tuberculosis
Tuberculosis is a chronic disease of many animal species and poultry caused by
bacteria of the genus Mycobacterium. It is characterized by development of
tubercles in the organs of most species. Bovine tuberculosis is caused by
Mycobacterium bovis. It is a significant zoonotic disease.

Transmission : An infected animal is the main source of transmission. The organisms

are excreted in the exhaled air and in all secretions and excretions. Inhalation is
the chief mode of entry and for calves infected milk is an important source of
infection. When infection has occurred tuberculosis may spread: a) by primary
complex (lesion at point of entry and the local lymph node) and b) by dissemination
from primary complex.

Antemortem findings:

Low grade fever

Chronic intermittent hacking cough and associated pneumonia
Difficult breathing
Weakness and loss of appetite
Emaciation
Swelling superficial body lymph nodes
Postmortem findings:

Tuberculous granuloma in the lymph nodes of the head, lungs (Fig. 68), intestine
and carcass. These have usually a well defined capsule enclosing a caseous mass
with a calcified centre. They are usually yellow in colour in cattle, white in
buffaloes and greyish white in other animals.
Active lesions may have a reddened periphery and caseous mass in the centre of a
lymph node.
Inactive lesions may be calcified and encapsulated
Nodules on the pleura and peritoneum
Lesions in the lungs (Fig. 69), liver, spleen, kidney
Bronchopneumonia
Firmer and enlarged udder, particularly rear quarters
Lesions in the meninges, bone marrow and joints
The diagnosis may be confirmed by making a smear of the lesion and with Ziehl-
Neelsen. The TB bacterium is a very small red staining bacillus.

Fig. 68: Tuberculous granuloma in the mediastinal lymph nodes. M. bovis was
isolated.

Fig. 69: Lesion of tuberculosis in the lungs.

Discussion : Mycobacteria invade cattle by respiratory (90 – 95 %) and oral routes

(5–10 %). Congenital infection in the bovine fetus occurs from an infected dam.
Tuberculosis lesions can be classified as acute miliary, nodular lesions and
chronic organ tuberculosis. Young calves are infected by ingestion of contaminated
milk. The incidence of human tuberculosis caused by Mycobacterium bovis has
markedly dropped with the pasteurization of milk. It also has dropped in areas
where programs of tuberculosis eradication are in place. Man is susceptible to the
bovine type. In cattle, lesions of tuberculosis caused by the avian type are
commonly found in the mesenteric lymph nodes. Tuberculosis in small ruminants is
rare. In pigs the disease may be caused by the bovine and avian types.
Superinfection is specific in cattle.
Judgement : Carcass of an animal affected with tuberculosis requires additional
postmortem examination of the lymph nodes, joints, bones and meninges. It is
suggested that the Codex Alimentarius judgement recommendations for cattle and
buffalo carcasses be followed.

Carcasses are condemned

where an eradication scheme has terminated or in cases of residual infection or re-

infection
in final stages of eradication - natural prevalence low
during early stages in high prevalence areas
Carcass of a reactor animal without lesions may be approved for limited
distribution. If the economic situation permits, this carcass should be condemned.
Heat treatment of meat is suggested during early and final stages of an eradication
programme: in low and high prevalence areas where one or more organs are affected,
and where miliary lesions, signs of generalization or recent haematogenous spread
are not observed. If the economical situation permits, then the carcass is
condemned.

In some countries, the carcass is approved if inactive lesions (calcified and/or

encapsulated) are observed in organs and without generalization in lymph nodes of
carcass.

Differential diagnosis : Lung and lymph node abscess, pleurisy. pericarditis,

chronic contagious pleuropneumonia, actinobacillosis, mycotic and parasitic
lesions, tumours, caseous lymphadenitis Johne's disease, adrenal gland tumour and
lymphomatosis

Johne's disease (Bovine paratuberculosis)

Johne's disease is a chronic, infectious bacterial disease of adult wild and
domestic ruminants such as cattle, sheep, and goats. It is characterized by the
thickening and corrugation of the wall of the intestine, gradual weight loss and
chronic diarrhoea and is caused by Mycobacterium paratuberculosis.

Transmission : Ingestion of faeces harbouring Mycobacterium paratuberculosis

The agent is persistent in soil, pasture, manure and stagnant water for prolonged
period.
Carrier animals, so called “faecal shedders”, are the most important source of
infection.
Ingestion of organism. Calves may become infected from a nursing infected dam.
Transmission with semen and in-utero are minor source of infection
Antemortem findings :

Incubation period 2 - 3 years with range from 6 months to 15 years.

Indifferent animal which stops eating at the end of the disease
Gradual and chronic weight loss and emaciation
Rough hair coat and dry skin
Non responsive diarrhoea with watery fluid faeces
Submandibular edema (“bottle jaw”)
Reduced milk production
Mastitis and infertility
Debility and death
Postmortem findings :

Thickened and corrugated intestinal mucosa (Fig. 70)

Enlarged caecal lymph nodes
Judgement : Carcass of an animal affected with Johne's disease is approved when
generalized systemic signs of disease are not present. A poor, thin and slightly
moist carcass should be held in the chiller and assessed after 24 or 48 hours. If
the dryness and setting of the carcass improves during this time it can be
released. The carcass with associated edema and emaciation is condemned.

Differential diagnosis : Other causes of diarrhoea and weight loss, malnutrition,

chronic salmonellosis, parasitism (e.g. Ostertagiasis), winter dysentery, Bovine
Viral Diarrhoea (BVD), “hardware” disease, coccidiosis, liver abscesses, kidney
disease, inflammation of the heart and its sac, toxic inflammation of the intestine
caused by arsenic, plants and mycotoxicosis and neoplasm.

Fig. 70 : Johne's disease. Thickened and corrugated intestinal mucosa.

Leptospirosis
Leptospirosis is an important and relatively common disease of domestic and wild
animals and humans. In cattle, it is manifested by interstitial nephritis, anaemia
and mastitis and abortion in most species. Leptospira spp. are the causative
agents.

Transmission : Animals contract the disease by eating and drinking leptospira-

contaminated urine, water, or by direct contact of broken skin or mucous membranes
with mud, vegetation or aborted fetuses of infected or carrier animals. Recovered
animals and animals with unapparent (subclinical) leptospirosis frequently excrete
billions of leptospiras in their urine for several months or years.

Antemortem findings :

Acute and subacute forms

Transient fever
Loss of appetite
Lactating cows may stop milking
Mastitis
Milk may be yellow, clotted and frequently blood stained
Severely affected animals

Jaundice and anaemia

Pneumonia
Abortion with frequent retention of the placenta (afterbirth)
Severe illness in young calves may be associated with yellowish discoloration of
mucous membranes and reddish-brown urine before death. The chronic form has mild
clinical signs and only abortion may be observed. If meningitis occurs, the animal
may show incoordination, salivation and muscular rigidity.

Postmortem findings :

Anaemia and jaundice

Subserosal and submucosal haemormage
Ulcers and haemorrhages in the abomasal mucosa
Rarely pulmonary edema or emphysema
Interstitial nephritis (Fig. 71)
Septicaemia

Fig. 71 : Leptospirosis. Interstitial nephritis in a bovine.

Judgement : Carcass of an animal affected with acute leptospirosis is condemned. A

chronic and localized condition may warrant an approval of the carcass.

Differential diagnosis : Acute and subacute forms to be differentiated from

babesiosis, anaplasmosis, rape and kale poisoning, bacillary haemoglobinuria, post
parturient haemoglobinuria and acute haemolytic anaemia in calves. The presence of
blood in the milk is a characteristic clinical sign which will differentiate
leptospirosis from other infectious diseases.

Discussion : Leptospirosis is a zoonosis and is also an occupational hazard for

farmers, veterinarians and butchers.

Human infection may occur by contamination with infected urine and urine contents.
The bacteria may be also found in milk in acute cases, however, it does not survive
for long period of time in milk. Pasteurization will also kill leptospiras. They
can survive for months in moist and humid environments, particularly in swamps,
ponds and streams or poorly drained pastures.

Brucellosis (contagious abortion, Bang's disease)

Brucellosis of cattle is an infectious, contagious disease caused by Brucella
abortus and is characterized by abortion in late pregnancy and a high rate of
infertility. B. melitensis affects goats, B. ovis sheep and B. suis swine. B.
abortus may occur in horses.

Transmission : An uninfected animal may become infected with Brucella organisms by

contaminated feed, pasture, water, milk, by an aborted fetus, fetal membranes and
uterine fluid and discharges. The disease may also be spread by dogs, rats, flies,
boots, vehicles, the milking machine and other equipment used in the barn. The
Brucella organism may be occasionally shed in urine.

Antemortem and postmortem findings :

In cattle

Abortion in non vaccinated pregnant cows in the last 3 - 4 months of pregnancy

Occasional inflammation of testes and epididymis
Swelling of scrotum (one or both sacs)
Edematous placenta and fetus
Hygromas on the knees (Fig. 72), stifles, hock and angle of the haunch, and between
the nuchal ligament and the primary thoracic spines.
In sheep

Fever, increased respiration and depression

Inferior quality of semen in rams
Edema and swelling of scrotum (see Fig. 163A in Chapter 5)
In chronic stage enlarged and hard epididymis, thickened scrotal tunics and
frequently atrophic testicles
Infertility in rams and abortion in ewes
Judgement: Cattle and horse carcasses affected with brucellosis are approved (after
removal of affected parts), as Brucella bacteria remain viable for only a short
period in the muscles after slaughter. In acute abortive form (after the
miscarriage), cattle carcasses are condemned. Pig, sheep, goat and buffalo
carcasses require total condemnation. Heat treatment may be recommended in some
areas for these species due to economic reasons. Affected part of the carcass,
udder, genital organs and corresponding lymph nodes must be condemned.

Reactor animals should be carefully handled during slaughter and dressing

procedures. Gloves and goggles should be worn when known reactors are being
slaughtered and hygroma lesions should be sprayed liberally with 1 % lactic acid at
meat inspection.

Differential diagnosis: Causes of abortion in cattle, IBR, vibriosis,

leptospirosis, trichomoniasis, mycoplasma infections, mycosis, nutritional and
physiological causes.

Discussion: Brucella organisms have only a short life in the muscles of slaughtered
animals. They are destroyed by lactic acid. While slaughtering and dressing the
reactors, a hook should be used in handling the uterus and udder. Employees in
close contact with infected animals should wear gloves and avoid accidental cuts.

In humans, brucellosis is called “Undulant Fever”. The general population is not at

risk with this disease if high levels of hygiene and sanitation are practised.
Pasteurized milk is brucella-free. Affected humans will suffer from intermittent
high fever, headache and generalized malaise.

Brucellosis is an important zoonosis in particular in rural areas in developing

countries and is an important occupational hazard for veterinarians, meat
inspectors, farmers, animal health inspectors and butchers.

Fig. 72: Brucellosis, Hygromas on the knee joints. This condition may be a sequel
to Brucella abortus infection.

Anthrax
Anthrax is a peracute disease of ruminants manifested with septicemia, sudden death
and tarry blood from the body openings of the cadaver. It is caused by Bacillus
anthracis.

Transmission: Man may contract anthrax by inhalation, ingestion and through a wound
in the skin. Biting flies have been shown to be transmitters.

Antemortem findings:

The peracute and acute forms in cattle and sheep are without clinical signs. Death
may follow in the acute form after 1 – 2 hours of illness. The acute form lasts
about 48 hours.

In pigs and horses this disease is usually localized and chronic and is often
characterized by swelling around the throat and head.

Antemortem findings in pigs:

Incubation 1 – 2 weeks
Edematous swelling of the throat and neck
Swallowing and breathing difficulties
Death due to choking or toxaemia
Septicemia is not observed.
Postmortem findings:

Dark-tarry blood discharge from body orifices

Absence of rigor mortis
Haemorrhage of the mucous and serous membranes, lymph nodes and subcutaneous tissue
Enlarged spleen
Severe haemorrhagic enteritis
Degeneration of the liver and kidneys
Bloating and rapid decomposition of carcass
Localized lesions in the intestine of pigs (dysentery)
Diagnosis of anthrax is carried out by direct microscopic examination of tissues
and fluids (Fig. 73).

Fig. 73: Anthrax. Toluidine blue stain. Bacillus anthracis in a bovine spleen.
Anthrax bacilli in tissue seen in short chains surrounded by a common capsule.

Judgement: Condemnation of the carcass and its parts by burning or burial. If

disposed by burial, the carcass should be buried at least 6 feet below ground. The
site should be surrounded by a foot thick layer of quicklime.

Differential diagnosis: Peracute blackquarter and septicaemic form of other

diseases. In splenic enlargement as seen in babesiosis, anaplasmosis and leucosis,
spleen consistency is firm. In anthrax, the spleen is soft and upon incision the
pulp exudes like thick blackish-red blood.

Discussion: If an animal has died from an unknown cause in an abattoir's pen or in

the stockyard, a blood smear from the tip of the ear should be examined to
eliminate anthrax as a cause of death. All measures should be taken to prevent
further contact with the carcass. The orifices of the nose, vulva and anus should
be packed with cotton swabs to eliminate further spillage of discharge. The carcass
must not be opened. Due to insufficient oxygen supply in the closed carcass, spores
of B. anthracis will not be formed and the organism will be killed. The spilled
discharge is firstly removed by drying with sawdust and sand and is then destroyed
together with the carcass. The carcass is wrapped in thick plastic sheets and
destruction is performed under the supervision of an appropriate government
official.

An open carcass facilitates exposure of B. anthracis to air and consequently,

spores are formed within a few hours. Anthrax spores are resistant to heat and
disinfectants and may survive in a suitable environment for years.

The abattoir's pen or stockyard area suspected of being in contact with an anthrax
animal should be disinfected with 10 % NaOH or 5 % formaldehyde and cleaned. This
cleaning should also include the cattle trucks or cars used for the transportation
of infected animals. All personnel that were in contact with anthrax or that
handled contaminated material, are also subjected to decontamination. The arms and
hands should be washed with liquid soap and hot water. After they have been rinsed,
they should be immersed for about one minute, in an organic iodine solution or 1
p.p.m. solution of mercuric perchloride or other acceptable agents. This is
followed by a potable water rinse. Clothing of the personnel involved should also
be cleaned and thoroughly disinfected by boiling.

If the carcass is discovered on the killing floor, all operations must cease. The
carcass and its parts including hides, hooves, viscera and blood must be condemned
and destroyed. The carcasses which have been dressed by the same abattoir employees
prior to or after the affected carcass must also be condemned and destroyed. Those
carcasses which had been dressed before the affected carcass may have a second
option of being salvaged with sterilization. They must be boiled for a minimum of 3
hours if contamination occurred with blood splashes. If impractical, these
carcasses may be used for “canned meat” for which heat treatment is recommended.

Disinfection of equipment used for the dressing of a diseased carcass as well as

the infected abattoir area, should be done with 5 % solution of sodium hydroxide
(NaOH). This disinfectant is used because of its action on fat and grease removal.
Heat in the form of a blowtorch can be used for disinfecting buildings.

Salmonellosis in bovine
Salmonellosis is a disease which occurs in all animals and humans. In animals,
salmonellosis is characterized clinically by one of three syndromes: a) peracute
septicemic form:, b) acute enteritis or c) chronic enteritis.

The young, old, debilitated and stressed animals are at greater risk. More then 200
antigenically different serotypes of Salmonella have been identified and all of
these possess pathogenic potential. The most frequently identified serotypes of the
organisms which cause the disease in cattle are S. typhimurium, S. dublin, S.
muenster and S. newport. Salmonellosis in stressed animals is frequently associated
with inadequate diet, irregular feeding, water deprivation, overcrowding,
parasitism, weather extremes, pregnancy, parturition, intercurrent diseases etc.
The calving complications which may predispose the disease include abortion or
early termination of pregnancy, retained placenta, endometritis and post-parturient
metabolic conditions.

Transmission: Ingestion of feed that have been contaminated by the faeces of

infected animals, by drinking water in stagnant ponds and by the carrier animals.
In housed animals, transmission is via contaminated feedstuff containing improperly
sterilized animal by-products such as bone and meat meal and fish meal. Casual
workers, infected clothing and utensils, transportation trucks and birds may
transmit the disease to the farm. Active carrier animals shed Salmonella organisms
intermittently and without obvious stress factors. Latent carriers with stress
factors are also identified in the transmission of salmonellosis.

Human infection is transmitted via contaminated water, raw milk and meat. Compared
to bovines, pigs and poultry are more significant sources of infection in humans
(see Chapter 4 and 7).

Antemortem findings:

Peracute septicemic form

Occurs most frequently in colostrum deficient animals up to four months of age.

Increased temperature 40.4°C – 41.5°C.
Depression
Diarrhoea and dehydration
Death within 24–48 hours
Approximately four weeks after the onset of diarrhoea

Polyarthritis
Meningoencephalitis
Necrosis of distal limbs, tails and ear
Acute enteritis

Common form in adult cattle in late pregnancy and early postpartum

High temperature of 40°C – 41°C
Depression and loss of appetite
Watery, foul smelling diarrhoea and dehydration
Emaciation
Reduced milk production and abortion
Death
Chronic enteritis - Preceded by acute enteric form

Further emaciation (poor doer), diarrhoea and dehydration

Fluctuating fever (35.5°C – 40.0°C)
Postmortem findings:

Septicemic form

Absence of gross lesions in animals

Submucosal and subserosal haemorrhage
Acute enteritis

Mucoenteritis to diffuse haemorrhagic enteritis

Severe necrotic enteritis of ileum and large intestine caused by S. typhimurium
Abomasitis in S. dublin infection
Enlarged, edematous and haemorrhagic lymph nodes
Thickened inflamed gall bladder wall
Fatty change of the enlarged liver
Subserous and epicardial haemorrhage
Chronic enteritis

Areas of necrosis in the wall of caecum and colon

Swollen mesenteric lymph nodes and spleen
Chronic pneumonia
In the septicemic and acute enteric forms, Salmonella organisms are present in the
blood, liver, bile, spleen, mesenteric lymph nodes and in intestinal content. In
the chronic form, bacteria is present in the intestinal lesions and less frequently
in other viscera.

Judgement: Carcass affected with Salmonellosis is condemned.

Differential diagnosis: Acute diarrhoea in calves: Diarrhoea caused by infections

(such as rotavirus, corona virus, cryptosporidiosis, E. coli), septicemia, dietetic
gastroenteritis, coccidiosis, Clostridium perfringens type C enterotoxaemia

Acute diarrhoea in adult cattle: Bovine viral diarrhoea, coccidiosis, “grain

overload”, gastrointestinal parasitism, winter dysentery, arsenic and lead
poisoning, bracken fern poisoning and intestinal obstruction

Chronic diarrhoea of adult cattle: Johne's disease, copper deficiency and

gastrointestinal parasitism

Haemorrhagic septicemia
Haemorrhagic septicemia is a systemic disease of cattle, buffalo, pigs, yaks and
camels. It is caused by Pasteurella multocida type B of Carter. Outbreaks of this
disease are associated with environmental stresses such as wet chilly weather and
overworked, exhausted animals. It is specific type of pasteurellosis distinct from
of other forms of pasteurella infections.

Transmission: By ingestion of contaminated feedstuff.

Antemortem findings:

Disease more severe in buffalo than in cattle

High fever up to 42°C
Salivation and difficulties in swallowing
Cough, and difficult breathing and associated pneumonia in later stages
Edematous swelling of throat, dewlap, brisket and peritoneum
Diarrhoea
Postmortem findings:

Subcutaneous swellings characterized with yellowish gelatinous fluid especially

around the throat region, brisket and perineum
Enlarged haemorrhagic lymph nodes
Haemorrhage in the organs
Pneumonia (Fig. 74)
Rarely haemorrhagic gastroenteritis
Petechial haemorrhage in the serous membranes which are extensive in some cases
Judgement: Carcass of an animal affected with haemorrhagic septicemia is condemned.
If the disease is diagnosed on antemortem examination, an animal should not be
allowed to enter the abattoir. Dressing of such a carcass would create potential
danger for the spread of infection to other carcasses.
Differential diagnosis: Anthrax, blackleg, acute leptospirosis, rinderpest, other
pasteurellosis, snake bite and lighting stroke.

Fig. 74: Haemorrhagic septicemia Fibrinous bronchopneumonia.

Calf diphtheria
Calf diphtheria is an acute oral infection of calves less than 3 months old. It is
caused by Fusobacterium (Sphaerophorus) necrophorum. This agent also causes liver
abscesses and “foot rot” in cattle.

Transmission: Fusobacterium necrophorum is an inhabitant of cattle's digestive

tract and the environment. Under unhygienic conditions, infection may be spread on
feeding troughs and dirty milk pails. Some of the contributory factors for
occurrence of this disease include abrasions in the oral mucosa, animals suffering
from poor nutrition and other (intercurrent) disease present in young calves.

Antemortem findings:

High temperature
Coughing
Loss of appetite and depression
Difficult breathing, chewing and swallowing
Swollen pharyngeal region
Deep ulcers on the tongue, palate, and inside of cheeks
Pneumonia
Postmortem findings:

Inflammation and ulceration with large masses of yellow-grey material in the mouth,
tongue, pharynx and larynx
Often aspiration pneumonia
Judgement: Carcass of an animal affected with local lesions is approved.
Generalized diphtheric lesions associated with pneumonia or toxaemia require the
carcass condemnation. The carcass is also condemned if lesions are associated with
emaciation.

Differential diagnosis: Vesicular diseases, neoplasms and abscesses

Actinobacillosis
Actinobacillosis is a chronic disease of cattle caused by Actinobacillus
lignieresi. It is manifested by inflammation of the tongue and less frequently
lymph nodes of the head and of even the viscera and carcass.

Antemortem findings:

Postmortem findings:

Enlarged tongue showing tough fibrous consistency. (“wooden tongue”)(Fig. 75)

A cluster of small yellowish nodules and erosions of tongue mucosa
Granulomatous lesions in the lymph nodes (Fig. 76)
Marked thickening of the lower part of oesophagus and stomach wall
Raised plaques and erosions in the mucosa of rumen and reticulum
Liver and diaphragm lesions due to contact spread from reticulum
Typical actinobacillosis lesions in the lymph nodes and organs consist of greenish-
yellow thick creamy pus with “sulphur granules”. These are bacterial colonies
surrounded by club like structures

Judgement: Carcass of an animal affected with active progressive inflammatory

lesions of actinobacillosis in lymph nodes and lung parenchyma is condemned.
Condemned material should be sent to authorized rendering plant. If the disease is
slight and confined to lymph nodes, the head and tongue and whole carcass are
approved after the condemnation of lymph nodes. If the tongue is diseased and no
lymph nodes are involved the head and carcass are approved. The tongue is
condemned.

Differential diagnosis: Neoplasms, tuberculosis, abscesses in the lymph nodes,

foreign body, salivary cysts, fungal granulomas, chronic pneumonia and parasites

Fig. 75: Actinobacillosis. Actinobacillosis of the tongue. The tongue is enlarged,

firm and contains numerous granulomatous lesions. It is called “wooden tongue”
because of its firmness due to diffuse proliferation of fibrous tissue.

Fig. 76: Actinobacillosis. Multifocal, well demarcated yellow lesions in the

retropharyngeal lymph node of a bovine animal.

Actinomycosis (“Lumpy Jaw”)

Actinomycosis is a chronic granulomatous disease of cattle and pigs and rarely in
sheep and horses. It is caused by Actinomyces bovis which is an obligatory parasite
in the mucous membrane of the mouth and pharynx. Infection occurs following injury
with a sharp object or hard feed pieces to the oral mucosa.

Antemortem findings:

Painful swelling of the maxilla and mandible (lumpy jaw); rarely in feet.
Suppurative tracts in the granulation tissue breaking towards oral cavity or skin
Ulceration of cheeks and gums and wart like granulations outward on head
Difficult breathing and salivation
Loss of weight
Diarrhoea and bloat
Postmortem findings:

Lesions in the mandible (Lumpy jaw) or maxilla (Fig. 77)

Granulomatous lesions in lower part oesophagus or anterior part of the reticulum
Local peritonitis
Mild abomasitis and enteritis
Judgement: see Actinobacillosis

Differential diagnosis: Tooth infection, impacted food, bone injury, neoplasms and
osteomyelitis due to other causes

Fig. 77: Actinomycosis. Diffuse granulomas in maxilla and formation of green yellow
pus. “Sulphur granules” are found in the pus.

Pyelonephritis (Contagious Bovine Pyelonephritis)

Pyelonephritis is a purulent and inflammatory bacterial disease of the kidney
pelvis and parenchyma caused by Corynebacterium renale. This disease is essentially
observed in adult cows and sows. A predisposing factor for developing a kidney
infection is trauma to the bladder and urethra during parturition.

Transmission : Infection is spread from clinically normal “carrier cows”. The

organism enters via vulva from: a) bedding contaminated with urine b) tail swishing
by “carrier cows” c) venereal transmission by infected bulls, and d) non sterilized
obstetrical instruments.

Antemortem findings :
Persistent increased temperature (39.5°C)
Loss of appetite and progressive weight loss
Painful urination and increased frequency of urination
Ammoniac odour from animal
Acute abdominal pain (colic)
Ceased rumen contraction
Decreased milk production
Postmortem findings :

Pyelonephritis showing enlarged, pale and greyish coloured kidney (Fig. 78) and
enlarged renal lymph nodes. Purulent lesion in the medulla, pelvis and ureters
Inflammation of kidney and kidney stones (uroliths, Fig. 79)
Enlarged renal lymph nodes
Uraemia
Judgement : It depends on infection of one or both kidneys and/or presence of a
urine odour. Carcass of an animal affected with pyelonephritis or nephritis is
condemned if : 1) renal insufficiency is associated with uraemia; 2) acute
infection of the kidney is accompanied with systemic changes in the organs and
lymph nodes, and/or degeneration of body tissues. Borderline cases with uraemic
odours should be kept in the chiller for 24 hours. They are subjected to a boiling
test. If a urinary odour is not present after detention, the carcass may be
approved.

Subacute or chronic kidney infections with no systemic changes allow for a

favourable judgement of carcass. Only the affected parts are condemned.
Pyelonephritis associated with kidney stones often has a favourable judgement of
the carcass.

Differential diagnosis : Enzootic haematuria in certain areas, post-parturient

haemoglobinuria, reticulitis, peritonitis, cystitis, metritis, leptospirosis,
Johne's disease, white spotted kidneys of calves, urinary obstruction, infarcts,
neoplasms and hydronephrosis

Fig. 78: Pyelonephritis (Contagoous) Bovine Pyelonephritis). Cut section of kidney

showing multifocal abscessation in the cortex and medulla.

Fig. 79: Pyelonephritis associated with urolithiasis (stones). Chemical analysis

revealed oxalate composition.

Metritis
Metritis is inflammation of the uterus. This condition is of bacterial origin. It
occurs as a result of calving problems such as retention of placenta, abortion,
twin births, abnormal labour and traumatic lesions of the uterus cervix and vagina.

Antemortem findings :

Postmortem findings :

Enlarged flaccid uterus showing “paint-brush” haemorrhages on the serosal surface

Inflammation of the uterus with light-brown foul smelling uterine exudate (Fig. 80)
Enlarged uterus containing greenish-yellow purulent exudate (Pyometra, Fig. 81)
Inflamed peritoneum at the entrance of the pelvic cavity
The iliac, lumbar and sacral lymph nodes are enlarged
Degeneration of the liver, kidney and heart muscles may be present
Congested musculature of the carcass
Necrosis of abdominal fat
Judgement : Carcass of an animal affected with acute metritis is condemned if it is
associated with septicemia or toxaemia. In chronic cases, when toxaemic signs are
lacking, the carcass may be approved if no antibiotic residues are found.

Differential diagnosis : Recent calving

Fig. 80: Metritis. Necrotizing inflammation of the uterus with greyish-brown foul
smelling uterine exudate.

Fig. 81: Pyometra. Enlarged uterus containing greenish -yellow exudate.

Mastitis
Mastitis is inflammation of the udder caused by bacteria, fungi and yeasts.
Depending on the virulence of the agent and resistance of the udder, mastitis is
manifested in acute or chronic forms.

Antemortem findings :

Variable temperature depending on stage of condition

Swollen warm, painful udder or hard enlargement involving one or all quarters
Depression, loss of appetite and dehydration
Abnormal gait caused by rubbing of the hind leg against inflamed quarter
Animal tends to lie down 6. Purulent or bloody exudate from teats or watery pale
fluid in chronic cases
Postmortem findings :

Pale yellow granular appearance of the udder parenchyma (Fig. 82)

Light brown edematous udder parenchyma (Fig. 83)
Enlarged supramammary, iliac and lumbar lymph nodes.
Injection sites
Judgement : Carcass and viscera are condemned if acute or gangrenous mastitis is
associated with systemic changes. If infection has spread from the supramammary
lymph nodes via the iliac lymph nodes to the lumbar lymph nodes, this can be taken
as evidence of spread of infection from its primary location. The condemnation of
the carcass may then be warranted. A localized condition of the udder has a
favourable judgement of the carcass.

Differential diagnosis : Edema, haematoma and rupture of the suspensory ligament

Fig. 82: Chronic mastitis. Enlarged, firm udder. Incision into the udder parenchyma
shows normal milk and pale yellow granular appearance of the udder parenchyma.

Fig. 83: Brown red edematous udder parenchyma. The udder culture resulted in a
heavy growth of Staphylococcus aureus.

Endocarditis
Endocarditis is inflammation of the endocardium of the heart. The lesion is most
commonly seen in the valves. It may be the result of bacteraemia caused by
infection in some remote organs such as the udder, uterus or other sites.

In cattle, the organisms most commonly associated with endocarditis are Actinomyces
pyogenes and Streptococcus spp.. Strains of Escherichia coli are also frequently
found. The lesion is most commonly found on the valves. Portions of vegetation may
become detached and released into the blood stream as emboli which may lodge in
other organs. They may be septic or aseptic. The latter contain thrombotic
material. Emboli, brought from the right heart to the lungs by blood vessels may
cause pulmonary abscesses, or pulmonary thrombosis and the emboli brought from the
left heart to the spleen and kidneys may cause septic or aseptic infarcts in these
organs. Abscesses in the heart may also be observed.

Antemortem findings :

Moderate fever
Breathing with accompanied grunt
Pallor of mucosae
Loss of condition and muscle weakness
Temporary fall in milk production in lactating animals
Jaundice and death
Postmortem findings :

Large cauliflower-like lesions (Fig. 84) in the endocardium

Small wart-like and verrucose lesions in the endocardium
Embolic lesions in other organs including the lungs, spleen, kidneys etc.

Fig. 84: Endocarditis. Vegetative valvular endocarditis.

Judgement: Carcass of a debilitated animal is condemned for verrucose endocarditis

if it is associated with lesions in lungs, liver or kidneys. Carcass affected with
ulcerative or verrucose endocarditis with no signs of systemic changes and negative
bacteriological result may be approved after heat treatment is applied.
Endocarditis showing scar tissue is approved. The heart is condemned.

Differential diagnosis : Pneumonia, pericarditis, pulmonary edema, emphysema,

pleuritis, lymphoma, high altitude disease, congenial heart disease, congenital
valvular heart cysts or deformities especially in calves.

Traumatic reticuloperitonitis (TRP, hardware disease, traumatic gastritis,

traumatic reticulitis)
TRP is caused from the perforation of the reticulum by a metallic foreign body. It
is mostly seen in adult dairy cattle and can occur in beef cattle.

Antemortem findings :

Sudden drop in milk production

Depression, loss of appetite and weight loss
Stretched head and neck
Reluctance to walk, arched back and tucked up abdomen
Scant, hard faeces, rarely covered with mucus.
Mild rumen bloat
Audible “grunt” in early stages
If mild septicemia develops the animal shows:

Elevated temperature (39.2 ° C - 40° C)

Increased heart rate
In chronic localized peritonitis, acute signs and pain lessen, temperature falls
and stomach reticulo-rumen motility may return.

Postmortem findings :

Adhesions of rumen, reticulum and peritoneum and abscessation

Acute or chronic peritonitis
Splenic abscessation
Traumatic pericarditis (Fig. 85)
Metallic objects such as nails, pieces of wire, magnets etc. in the reticulum
Lung abscessation or pneumonia
Septic pleuritis
Edema of the chest
Judgement : Viscera and carcass are condemned - a) if the animal is affected with
acute diffuse peritonitis or acute infectious pericarditis associated with
septicemia; b) carcass with traumatic pericarditis associated with fever, large
accumulation of exudate, circulatory disturbances, degenerative changes in organs,
or abnormal odour. c) carcass with chronic traumatic reticulo-peritonitis and/or
purulent pericarditis with associated pleuritis, abscessation and edema of the
chest.

Chronic adhesive localized peritonitis and chronic pericarditis without systemic

changes in well nourished animals allow a favourable judgement of the carcass. The
affected parts of the carcass and organs are condemned.

A carcass affected with infectious exudative pericarditis in a subacute stage may

be conditionally approved pending heat treatment, if bacteriological and antibiotic
residue findings are negative.

Differential diagnosis : Uterine or vaginal trauma, abomasal ulceration with

perforation, liver abscessation, pyelonephritis, ketosis, abomasal displacement and
volvulus, and “grain overload”.

Fig. 85: TRP. Cross section of the heart reveals thick fibrinous deposits that
encircled heart. Rusty nail has penetrated through the wall of the reticulum into
the pericardium in this case.