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    Book1 (Recovered)

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    Ali
    This document contains data from an experiment measuring absorbance of a compound (PNP) at different concentrations using UV-Vis spectroscopy. It shows the absorbance measurements for 5 standard PNP solutions with known concentrations ranging from 10 to 75 μM along with measurements for an unknown sample. A linear calibration curve is generated that relates the standard concentrations to their average absorbances. The unknown sample concentration is then determined using the calibration curve equation. A second set of data examines the effect of pH on absorbance of the compound.

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    © All Rights Reserved
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    Book1 (Recovered)

    Uploaded by

    Ali
    16 views5 pages
    This document contains data from an experiment measuring absorbance of a compound (PNP) at different concentrations using UV-Vis spectroscopy. It shows the absorbance measurements for 5 standard PNP solutions with known concentrations ranging from 10 to 75 μM along with measurements for an unknown sample. A linear calibration curve is generated that relates the standard concentrations to their average absorbances. The unknown sample concentration is then determined using the calibration curve equation. A second set of data examines the effect of pH on absorbance of the compound.

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    This document contains data from an experiment measuring absorbance of a compound (PNP) at different concentrations using UV-Vis spectroscopy. It shows the absorbance measurements for 5 standard PNP solutions with known concentrations ranging from 10 to 75 μM along with measurements for an unknown sample. A linear calibration curve is generated that relates the standard concentrations to their average absorbances. The unknown sample concentration is then determined using the calibration curve equation. A second set of data examines the effect of pH on absorbance of the compound.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    16 views5 pages

    Book1 (Recovered)

    Uploaded by

    Ali
    This document contains data from an experiment measuring absorbance of a compound (PNP) at different concentrations using UV-Vis spectroscopy. It shows the absorbance measurements for 5 standard PNP solutions with known concentrations ranging from 10 to 75 μM along with measurements for an unknown sample. A linear calibration curve is generated that relates the standard concentrations to their average absorbances. The unknown sample concentration is then determined using the calibration curve equation. A second set of data examines the effect of pH on absorbance of the compound.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
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    Standard [PNP] (μM) Trial 1(A) Trial 2 (A)

    Standard 1 10 0.039 0.039


    Standard 2 20 0.075 0.075
    Standard 3 30 0.127 0.127
    Standard 4 50 0.201 0.201
    Standard 5 75 0.314 0.314
    Unknown 0.332 0.332

    Standard [PNP] (μM) Average Ab. Standard Dev.


    Standard 1 10 0.039 0
    Standard 2 20 0.075 0
    Standard 3 30 0.127 0
    Standard 4 50 0.201 3.399349889E-17
    Standard 5 75 0.314 0
    Unknown 0.3316666666667 0.0005773502692
    Trial 3 (A)
    0.039
    0.075
    0.127
    0.201
    0.314
    0.331

    x y
    0 0.332
    80.285 0.332
    80.285 0

    PNP Concentration Vs Absorbance


    0.35
    f(x) = 0.00422686567164179 x − 0.00519402985074624
    R² = 0.998272364908395 5; 0.314
    0.3

    0.25

    0.2 4; 0.201 Standard Sample


    Absorbance

    Linear (Standard Sample)


    0.15 Unknown Sample
    3; 0.127
    0.1
    2; 0.075
    0.05
    1; 0.039

    0
    0 10 20 30 40 50 60 70 80 90
    PNP Concentration
    Standard Sample
    Linear (Standard Sample)
    Unknown Sample
    pH Trial 1(A) Trial 2 (A) Trial 3 (A)
    4 0.07 0.06 0.06
    6 0.038 0.038 0.038
    7 0.207 0.207 0.207
    7.64 0.351 0.351 0.351
    8 0.367 0.368 0.368
    8.43 0.458 0.458 0.458
    9 0.388 0.388 0.388
    10 0.443 0.443 0.443
    Mid Point = 0.21 Pka =

    pH Vs Absorbance
    0.500
    0.450
    0.400
    0.350
    Absorbance

    0.300
    0.250
    0.200
    0.150
    0.100
    0.050
    0.000
    3 4 5 6 7 8 9 10
    pH Values
    Standard
    Average Ab.
    Deviation
    0.063 0.006
    0.038 0.000
    0.207 0.000
    0.351 0.000
    0.368 0.001
    0.458 0.000
    0.388 0.000
    0.443 0.000
    6.706

    sorbance

    7 8 9 10 11
    H Values

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