February 2019

QIAsymphony SP ®

Protocol Sheet
Casework_1000_V8 and Casework_1000_H2O_V8 protocol
This document is the Casework_1000_V8 and Casework_1000_H2O_V8 QIAsymphony SP Protocol Sheet, R1, for
QIAsymphony DNA Investigator® Kit.

Sample to Insight__
General information

The QIAsymphony DNA Investigator Kit is intended for molecular biology applications. This product
is not intended for the diagnosis, prevention, or treatment of a disease.

These protocols are for purification of total DNA from samples encountered in forensic, human
identity, and biosecurity applications using the QIAsymphony SP and the QIAsymphony DNA
Investigator Kit.

Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can
vary greatly, there is also a variety of different pretreatments, optimized for specific sample types.
For the CW_1000_V8 and CW_1000_H2O_V8 protocol, samples are lysed under denaturing
conditions in the presence of proteinase K and Buffer ATL in a total volume of 1000 µl.

The Elution can be performed either with the buffer ATE (CW_1000_V8) or with the buffer AVE
(CW_1000_H2O_V8).

Note: It is the user’s responsibility to validate performance using this combination for any procedures
used in their laboratory.

CW_1000_H2O_V8

Kit QIAsymphony DNA Investigator Kit

Sample material Large-volume forensic samples

Protocol name CW_1000_H2O_V8

Default Assay Control Set ACS_CW_1000_H2O_V8

Elution solution Buffer AVE

Elution volume 100 µl, 150 µl, 200 µl

Required software version Version 5.0 or higher

QIAsymphony SP Protocol Sheet ─ Casework_1000_V8 protocol 02/2019 2

CW_1000_V8

Kit QIAsymphony DNA Investigator Kit

Sample material Large-volume forensic samples

Protocol name CW_1000_V8

Default Assay Control Set ACS_CW_1000_V8

Elution solution Buffer ATE

Elution volume 100 µl, 150 µl, 200 µl

Required software version Version 5.0 or higher

Materials required but not provided

For all sample types

 Vortexer
 Thermomixer or shaker-incubator

“Sample” drawer

Sample type Large-volume forensic samples

Sample volume 1000 µl

Primary sample tubes See www.qiagen.com/goto/qsdnainvestigator for more information

Secondary sample tubes See www.qiagen.com/goto/qsdnainvestigator for more information

Inserts See www.qiagen.com/goto/qsdnainvestigator for more information

Other n/a

n/a = not applicable.

“Reagents and Consumables” drawer

Position A1 and/or A2 Reagent cartridge (RC)

Position B1 n/a

Tip rack holder 1–17 Disposable filter-tips, 200 µl

Tip rack holder 1–17 Disposable filter-tips, 1500 µl

Unit box holder 1–4 Unit boxes containing sample prep cartridges

Unit box holder 1–4 Unit boxes containing 8-Rod Covers

n/a = not applicable.

QIAsymphony SP Protocol Sheet ─ Casework_1000_V8 protocol 02/2019 3

“Waste” drawer

Unit box holder 1–4 Empty unit boxes

Waste bag holder Waste bag

Liquid waste bottle holder Liquid waste bottle

“Eluate” drawer

Elution rack (we recommend using slot 1, cooling position) See www.qiagen.com/goto/qsdnainvestigator for more
information

Required plasticware

One batch, 24 samples* Four batches, 96 samples*

Disposable filter-tips, 200 µl†‡ 28 112

Disposable filter-tips, 1500 µl †‡

56 224

Sample prep cartridges§ 15 60

8-Rod Covers¶ 3 12

* Use of less than 24 samples per batch decreases the number of disposable filter-tips required per run.
†
There are 32 filter-tips/tip rack.
‡
Number of required filter-tips includes filter-tips for 1 inventory scan per reagent cartridge.
§
There are 28 sample prep cartridges/unit box.
¶
There are twelve 8-Rod Covers/unit box.

Note: Numbers of filter-tips given may differ from the numbers displayed in the touchscreen
depending on settings, for example, number of internal controls used per batch.

Preparation of sample material

When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective
goggles. For more information, consult the appropriate material safety data sheets (MSDSs),
available from the product supplier.

Important points before starting

 QIAsymphony magnetic particles copurify RNA and DNA if both are present in the sample. If
RNA-free DNA is required, add RNase A to the sample in the step indicated in the respective
pretreatment protocol.
 Before beginning the procedure, read “Important Notes”, page 12 of the QIAsymphony DNA
Investigator Handbook.

QIAsymphony SP Protocol Sheet ─ Casework_1000_V8 protocol 02/2019 4

Large-volume samples

This protocol is for isolation of total (genomic and mitochondrial) DNA from forensic samples that
require increased volumes for thorough lysis, e.g., diffuse stains on fabric or paper. The
pretreatment includes lysis of samples using proteinase K.

Things to do before starting

 Before using Buffer ATL, check that it does not contain a white precipitate. If necessary,
incubate for 30 minutes at 70°C with gentle agitation.
 Set a thermomixer or shaker–incubator to 56°C for use in step 4.
 If processing semen samples, prepare an aqueous 1 M DTT* stock solution. Store aliquots
at –20°C. Thaw immediately before use.

Pretreatment protocol for large-volume samples

1. Transfer samples to a 2 ml microcentrifuge tube (not provided). Large samples can be cut
into smaller pieces to fit more conveniently.
2. Add 960 µl Buffer ATL.
3. Add 40 µl proteinase K, and mix by vortexing. If processing semen samples, add 40 µl 1 M
DTT.
4. Place the tube in a thermomixer or heated orbital incubator, and incubate with shaking at
900 rpm at 56°C for at least 15 min.
5. Carefully transfer the lysate to sample tubes or plates that are compatible with the sample
rack of the QIAsymphony SP.
See www.qiagen.com/QIAsymphony/Resources for a full list of compatible vessels. We
recommend using 2 ml tubes (e.g., Sarstedt, cat. no. 72.693 or 72.608) or S-Blocks
(cat. no. 19585).
Note: Do not transfer any solid material as this may clog the tips during automated DNA
purification.
Lysate remaining in solid sample material (e.g., denim) can be harvested by transferring the
material to a QIAshredder spin column (not supplied) and centrifuging at full speed for
2 min in a microcentrifuge. Transfer the flow-through to the sample tube or plate.
6. Continue with the protocol “DNA Purification from Casework and Reference Samples”
(page 19 in the QIAsymphony DNA Investigator Handbook).

* When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more
information, consult the appropriate material safety data sheets (MSDSs), available from the product supplier.

QIAsymphony SP Protocol Sheet ─ Casework_1000_V8 protocol 02/2019 5

Revision history

Document revision history

R1 02/2019 Update for QIAsymphony Software version 5.0

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN®
kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at
www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.

Trademarks: QIAGEN®, Sample to Insight®, Investigator®, QIAsymphony® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered
unprotected by law.
02/2019 HB-0986-S41-001 © 2019 QIAGEN, all rights reserved.

QIAsymphony SP Protocol Sheet ─ Casework_1000_V8 protocol 02/2019 6

Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com

QIAsymphony SP Protocol
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