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    CARBOHYDRATES Lab

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    Jan Leanne Orbigoso
    The document discusses various tests used to detect the presence of monosaccharides, disaccharides, and polysaccharides. Monosaccharides like glucose give positive results in Fehling's test, Benedict's test, Barfoed's test, and Molisch's test. Disaccharides like sucrose do not produce positive results in Fehling's, Benedict's or Barfoed's tests. The iodine test produces a positive result for starch, indicating the presence of a polysaccharide.

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    CARBOHYDRATES Lab

    Uploaded by

    Jan Leanne Orbigoso
    28 views22 pages
    The document discusses various tests used to detect the presence of monosaccharides, disaccharides, and polysaccharides. Monosaccharides like glucose give positive results in Fehling's test, Benedict's test, Barfoed's test, and Molisch's test. Disaccharides like sucrose do not produce positive results in Fehling's, Benedict's or Barfoed's tests. The iodine test produces a positive result for starch, indicating the presence of a polysaccharide.

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    CARBOHYDRATES-Lab

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    The document discusses various tests used to detect the presence of monosaccharides, disaccharides, and polysaccharides. Monosaccharides like glucose give positive results in Fehling's test, Benedict's test, Barfoed's test, and Molisch's test. Disaccharides like sucrose do not produce positive results in Fehling's, Benedict's or Barfoed's tests. The iodine test produces a positive result for starch, indicating the presence of a polysaccharide.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    28 views22 pages

    CARBOHYDRATES Lab

    Uploaded by

    Jan Leanne Orbigoso
    The document discusses various tests used to detect the presence of monosaccharides, disaccharides, and polysaccharides. Monosaccharides like glucose give positive results in Fehling's test, Benedict's test, Barfoed's test, and Molisch's test. Disaccharides like sucrose do not produce positive results in Fehling's, Benedict's or Barfoed's tests. The iodine test produces a positive result for starch, indicating the presence of a polysaccharide.

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    CARBOHYDRATES

    Presented by:
    Marilyn G. Dagalea
    Objective
    • To detect the presence of Monosaccharide, disaccharides and
    polysaccharides using different tests.
    Monosaccharide and

    Disaccharides Moore’s
    Test (Influence of
    Concentrated Alkali)
    1. Mix 1 ml of 5% glucose
    with 1ml concentrated
    NaOH.
    2. Boil and note the change
    of color and odor
    produced.

    5% Glucose Soln.

    Conc. NaOH

    Positive Result
    Molisch’s Test

    (a-napthol reaction) 1.
    Place 1ml of 5 % glucose
    solution in a test tube
    2. Add 1 drop of Molisch’s
    reagent and mix
    thoroughly.
    3. Incline the tube and allow
    1 ml of conc. Sulfuric acid
    to flow on the side of the
    tube. (Note: Do not
    shake).

    Sulfuric acid
    5% Glucose Soln.
    Molisch’s Reagents

    Positive Result

    Fehling’s Test
    1. Place 5 ml each of Fehling’s A and
    Fehling’s B in a test tube
    Reduction Test 2. Dilute it with 4 cc of water
    3. Boil 1 ml of this mixture in a water
    bath ( if change in color is observed,
    it indicates contamination. Discard
    and get a freshly prepared solution)
    Drop of
    4. If color is unchanged after boiling, glucose Positive Result
    add 5% glucose solution drop by
    drop heating the mixture after each
    dilution.
    5. Note the changes produced.

    Fehling’s A Fehling’s B
    Benedict’s Test
    Glucose Soln.

    1. Mix 1 ml of Benedict’s
    reagent with 2 drops of
    glucose
    solution.
    2. Boil
    for 2 Benedict’s reagent
    minutes
    and
    allow to cool.
    3. Observe for any change in color.
    Positive Result
    of 50% glucose with 0.1 ml of
    Nylander’s
    reagent
    Nylanders Test 2. Heat for 5 minutes in a water bath
    and
    1. Mix 1 ml note the result.
    Glucose Soln.

    Positive Result
    Nylander’s Reagent

    Barfoed’s Test
    Glucose Soln.

    1. Mix 1 ml of Barfoed’s
    reagent with 0.1 ml of
    5% glucose solution.
    2. Heat for
    30 seconds
    and allow
    to stand for
    15 minutes.

    Barfoed’s reagent
    Positive Result

    2. Warm and note the result.


    Picric
    acid Test
    1. To 1 ml
    of 5% Glucose Soln. Picric acid Soln. Sodium
    glucose Carbonate

    solution
    add 0.5 ml
    of
    saturated
    picric acid
    solution and 0.2 ml of 10% Sodium
    Carbonate.
    Positive Result
    Seliwanoff’s Test
    1. Place 1 ml of Seliwanoff’s
    reagent in each of 6 test tube.
    2. To the first test tube , add 3
    drops of 2% freshly
    prepared
    solution of fructose, to the

    2nd test tube add glucose, to


    the 3rd test tube add
    galactose, to the 4th test tube
    add sucrose, to the 5th test
    tube add maltose and to the
    th
    6 test tube add lactose.
    3. Boil in a water bath
    4. Note the color produced and
    record the time required for
    the development of pink in
    each of the tubes.
    Positive Result

    Polysaccharides piece of white paper 2. Drop of Lugol’s


    solution on it.
    Iodine test 3. Note the result.
    on Starch
    Powder
    1. Place a
    small

    pinch of starch on a glass over a


    Starch Positive Result

    Powder Iodine Solution

    Summary
    Test Positive results
    Moore’s test Maroon –orange with caramel odor
    Molisch’s test Violet ring at the junction of two liquids
    Fehling’s test Yellow to brick red ppt.
    Benedict’s test Yellow to brick red ppt.
    Nylander’s test Black metallic bismuth
    Barfoed’s test Reddish ppt of copper oxide
    Picric acid test Mahogany red ppt.
    Seliwanoff’s test Red color /solution
    Mucic acid test Crystal formation

    Sugar solution Mucic acid Fehling’s test Benedict’s test Barfoed’s test

    Glucose - + + +
    Fructose - + + +
    Galactose + + + +
    Maltose - + + -
    Sucrose - - - -
    lactose + + + -

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