TrekAvidin-HRP Label ISO

9001:2000
Conjugated Streptavidin Horseradish Peroxidase
CERTIFIED
Detection Component
Control Number: 901-STHRP700-010611

Catalog Number: STHRP700 L10 Species Reactivity:

Description: 110 ml N/A
Storage and Stability:
Intended Use: Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
For In Vitro Diagnostic Use stored under conditions other than those specified in the package insert, they must be
Summary & Explanation: verified by the user.
Protocol Recommendations:
TrekAvidin (streptavidin) is a protein that has similar binding properties to egg white
avidin. It is isolated from streptomyces avidinii. Streptavidin has a molecular weight Deparaffinization:
of 60 kDa and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is Deparaffinize slides in Slide Brite or xylene. Hydrate slides in a series of graded
a water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10 alcohol to water.
-15) for biotin. Streptavidin conjugated to horseradish peroxidase has proven useful in Peroxide Block:
the detection of antigens coupled with biotinylated secondary antibodies. Block for 5 minutes with Biocare's Peroxidazed 1.
There are several advantages when using streptavidin-conjugates versus an ABC Pretreatment Solution/Protocol:
complex. In contrast to avidin, streptavidin is not glycosylated and is therefore Please refer to the respective primary antibody datasheet for recommended
uncharged at neutral pH (6.5 versus 10). This lowers nonspecific background staining. pretreatment solution and protocol.
Streptavidin also lacks carbohydrate side chains that may be another cause of non- Protein Block:
specific background. Another key advantage of streptavidin is the significant increase Incubate for 10-15 minutes at RT with Biocare's Background Sniper.
in sensitivity (probably due to less steric hindrance), thus facilitating an increase in Primary Antibody:
overall binding capacity. Please refer to the respective primary antibody datasheet for incubation time.
Streptavidin-HRP is much more stable than the ABC complex. The ABC complex Link:
must be freshly made 30 minutes prior to use, and is stable only for a few days. In Incubate for 20 minutes at RT with Trekkie Link.
contrast, the Streptavidin-HRP reagent is very stable, color-coded, and can be stored
Label:
for up to 1-2 years. Biocare’s Streptavidin-HRP comes in a ready-to-use format, thus
Incubate for 10 minutes at RT with TrekAvidin-HRP Label.
saving time and potential mistakes.
The TrekAvidin-HRP is part of Biocare’s Starr Trek detection system. It is extremely Chromogen:
cost effective and can be used with Biocare’s, BioGenex’s or Dako’s concentrates and Incubate for 5 minutes at RT when using Biocare's DAB.
prediluted antibodies. Counterstain:
Rinse with deionized water. Incubate for 30-60 seconds with Tacha's Automated
Known Applications: Hematoxylin. Rinse with deionized water. Apply Tacha's Bluing solution for 1 minute.
Immunohistochemistry (formalin-fixed paraffin-embedded tissues) Technical Notes:
Supplied As: 1. Use TBS for washing steps.
Peroxidase-conjugated streptavidin in PBS and preservative Protocol Notes:
Materials and Reagents Needed But Not Provided: The optimum antibody dilution and protocols for a specific application can vary due to
Microscope slides, positively charged many factors. These include, but are not limited to: fixation, incubation times, tissue
Desert chamber* (Drying oven) section thickness and detection kit used. The data sheet’s recommendations and
Positive and negative tissue controls protocols are based on exclusive use of Biocare products. Due to the superior
Xylene (Could be replaced with a xylene substitute*) sensitivity of these unique reagents, the recommended incubation times and titers listed
Ethanol or reagent alcohol are not applicable to other detection systems, as results may vary. Ultimately, it is the
Decloaking chamber* (Pressure cooker) responsibility of the investigator to determine optimal conditions.
Deionized or distilled water Performance Characteristics:
Wash buffer*(TBS/PBS) The protocols for a specific application can vary. These include, but are not limited to:
Pretreatment Reagents* fixation, heat-retrieval method, incubation times, tissue section thickness and detection
Enzyme Digestion* kit used. Due to the superior sensitivity of these unique reagents, the recommended
Avidin-Biotin Blocking Kit* (Labeled Streptavidin Kits Only) incubation times and titers listed are not applicable to other detection systems, as
Peroxidase block* results may vary. The data sheet recommendations and protocols are based on
Protein block* exclusive use of Biocare products. Ultimately, it is the responsibility of the
Primary antibody* investigator to determine optimal conditions. These products are tools that can be used
Negative Control Reagents* for interpretation of morphological findings in conjunction with other diagnostic tests
Chromogens* and pertinent clinical data by a qualified pathologist.
Hematoxylin*
Quality Control:
Bluing Reagent*
Mounting media* Refer to NCCLS Quality Assurance for Immunocytochemistry approved guidelines,
December 1999 MM4-A Vol.19 No.26 for more information on tissue controls.
* Biocare Medical Products: Refer to a Biocare Medical catalog for further information
regarding catalog numbers and ordering information. Certain reagents listed above are
based on specific application and detection system used.

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 TrekAvidin-HRP Label ISO
9001:2000
Conjugated Streptavidin Horseradish Peroxidase
CERTIFIED
Detection Component
Control Number: 901-STHRP700-010611

Precautions:
This product is not classified as hazardous. The preservative used in this reagent is
Proclin 950 and the concentration is less than 0.25%. Overexposure to Proclin 950 can
cause skin and eye irritation and irritation to mucous membranes and upper respiratory
tract. The concentration of Proclin 950 in this product does not meet the OSHA
criteria for a hazardous substance. Wear disposable gloves when handling reagents.
Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water. Microbial contamination of reagents may
result in an increase in nonspecific staining. Incubation times or temperatures other
than those specified may give erroneous results. The user must validate any such
change. The MSDS is available upon request.
Consult OSHA, federal, state or local regulations for disposal of any toxic substances.
ProclinTM is a trademark of Rohm and Haas Company, or of its subsidiaries or
affiliates.
Troubleshooting:
Follow the reagent specific protocol recommendations according to data sheet
provided. If atypical results occur, contact Biocare's Technical Support at 1
-800-542-2002.
Limitations & Warranty:
There are no warranties, expressed or implied, which extend beyond this description.
Biocare is not liable for property damage, personal injury, or economic loss caused by
this product.

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