 Topic: Potency Determination of Ciprofloxacin by

Using UV Spectroscopy.

 Submitted To:

Ms. Nazneen Ahmeda sultana

Lecturer (Sr. Scale)
Department of Pharmacy
Faculty of Allied Health Sciences
Daffodil International University
Dhaka, Bangladesh
Email: nazneen.ph@diu.edu.bd

 Submitted By:
Name: Rifat Ahmed Tanvir
Student ID: 201-29-399
Section: 23-PCB
Email: rifat29-399@diu.edu.bd

Daffodil International University

Assignment
Course Information:
Name of the Course: Pharmaceutical Analysis & Quality Control-II
Course Code : BPH-311
 Program : B. Pharm

Introduction
Ciprofloxacin is an established broad-spectrum fluoroquinolone
antibiotic displaying activity against both Gram-positive and Gram-
negative bacteria pathogens. It acts principally by inhibiting DNA-
gyrase and topoisomerase IV present in bacterial cells which are
responsible for reproduction of DNA of bacteria. It is particularly
effective against Pseudomonas aeruginosa. Inpatientswith
cysticfibrosis and bronchiectasis, P. aeruginosa causes severe
decline in lung function .its oral and parenteral dosage forms
indicated for the treatment of exacerbations of respiratory tract
infection. Ciprofloxacin is used in various clinical diseases such
as infectious enteritis and inflammatory bowel disease, induction
of nitric oxide, suppression of pro-inflammatory cytokines and
moreover it is able to induce apoptosis in a variety of human
cancer cell lines including the human colonic cancer cells. In
critically ill patients without renal impairment ciprofloxacin doses
ranging from 200 mg (twice daily) to 400 mg (three times daily)
have been used whereas In patients with severely impaired renal
function dosage adjustment is advised i.e. a 50% dosage reduction
has been recommended . Ciprofloxacin oral absorption can be
significantly reduced after concomitant administration of foods
containing Ca2+ and Al3 (divalent and trivalent cations) and
milk.

Figure 1: Structure of ciprofloxacin

 Wavelength Selection
Ciprofloxacin solution of about 200 ppm was accurately prepared
in water. This solution was scanned in the 200-400 nm UV
regions. The wavelength maximum (λmax) was observed at 278
nm and this wavelength was adopted for absorbance
measurement.

Standard Stock solution

For standard stock solution accurately weighed 20 mg of
ciprofloxacin and it transferred to a volumetric flask and add
sufficient water to produce 100 ml.

Sample Preparation
The six different brands of ciprofloxacin i.e. Novidat Axcin Cyrocin
Cycin Quinoflox Ciproxin were purchased from different
pharmacies situated in Karachi, Pakistan. Ciprofloxacin tablets of
each brand have same batch number and were labeled to contain
ciprofloxacin 500 mg per tablet. Accurately weigh 20 tablets of
each brand of ciprofloxacin and crushed uniformly with the help of
a mortar and pestle. By calculating the average weight, weigh
sample powder equivalent to 20 mg of ciprofloxacin and
transferred into a volumetric flask and make up volume upto 100
ml with water.
 Dilutions preparation
From the standard stock solution of different brands of
ciprofloxacin i.e. Novidat, Axcin, Cyrocin, Cycin ,Quinoflox and
Ciproxin different dilutions are prepared. Five different dilutions of
each brand of 100 ppm, 50 ppm , 25 ppm 12.5ppm and 6.25ppm
were prepared for each brand from 200ppm standard stock
solution.

Procedure
After preparation of standard and sample solutions, measure the
absorbance of standard solution (200 ppm in 100 ml) and different
dilutions (100 ppm, 50 ppm , 25 ppm12.5 ppm and 6.25 ppm, all
in 100 ml) in 1cm cuvette by using UV-VIS spectrophotometer at
the wavelength of maximum absorbance 278 nm, using the blank
solution. Calculate the quantity in mg, of ciprofloxacin per tablet.

Results and Discusion

The aim of the study was to carry out the pharmaceutical assay on
different brands (NOVIDAT, AXCIN, CYROCIN, CYCIN,
QUINOFLOX and CIPROXIN) of ciprofloxacin tablets by using
spectrophotometer. Pharmaceutical company name, Brand names,
average wt of tablets, weight for 100ppm in 100 ml,
absorbance table 1 and % assay are shown in table 1, 2 and
figure 2. Five dilutions of 100ppm, 50ppm, 25ppm, 12.5ppm and
6.25ppm for each brand of ciprofloxacin were prepared. Their
absorbance was taken to calculate the percentage assay,
regression equation and to obtain the regression line to predict
further availability of ciprofloxacin. The linearity was detected by
preparing solution of 100ppm, 50ppm, 25ppm, 12.5ppm and
6.25ppm of each brand and absorbance were taken in triplicate
manner at each level by spectrophotometric analysis.
Concentration vs. absorbance at level of 100ppm, 50ppm
,25ppm, 12.5ppm and 6.25ppm were plotted on graph for linearity
detection shown in figure 3-8 which conclude that it obeys Beer’s
Lambert law i.e. absorbance is directly proportion to
concentration.

Table 1: Different brands of ciprofloxacin

Average wt for
Pharma Brand wt of 100 ppm Absorbance % assay
Name tablet g in 100 at 278 nm
ml

Sami Novidat 0.7620 0.0152 0.125 100.00

Sandoze Axcin 0.7693 0.0153 0.127 101.60
Highnoon Cyrocin 0.8666 0.0173 0.178 142.40
Ildong Cycin 0.772 0.0172 0.124 99.20
Bosh Quinoflox 0.7529 0.0159 0.156 124.80
Bayer Ciproxin 0.7643 0.0152 0.171 136.80
 Table 2: Assay of different brands of
ciprofloxacin
Brand Name % Assay
Novidat 100
Axcin 101.6
Cyrocin 142.4
Cycin 99.2
Quinoflox 124.8
Ciproxin 136.8

Fig. 2 :% Assay of different brands of ciprofloxacin

Table 3: Absorbance of different brands

Con. A B C D E F
100 0.125 0.127 0.178 0.156 0.16 0.171
50 0.0625 0.066 0.092 0.075 0.08 0.088
25 0.033 0.033 0.045 0.035 0.04 0.045
12.5 0.017 0.015 0.022 0.017 0.014 0.018
6.25 0.008 0.007 0.001 0.008 0.007 0.008
Table 4: Regression equations of different
brands
Brands Regression equations R²
A y = 0.0012x + 0.0011 0.9998
B y = 0.0013x - 4E-05 0.9992
C y = 0.0018x - 0.0038 0.9996
D y = 0.0016x - 0.0032 0.9996
E y = 0.0016x - 0.0035 0.9989
F y = 0.0017x - 0.0014 0.9986

Figure 3: linearity plot for assay of different dilutions of Novidat,

Figure 4: linearity plot for assay of different dilutions of Axcin

 Figure 5: Linearity plot for assay of different dilutions of Cyrocin

Figure 6: Linearity plot for assay of different dilutions of Cycin

Figure 7: Linearity plot for assay of different dilutions of Quinoflox

Figure 8 : Linearity plot for assay of different dilutions of Ciproxin

List of Figure:
Figure No : 01 Structure of ciprofloxacin
Figure No : 02 % Assay of different brands of
ciprofloxacin
Figure No : 03 linearity plot for assay of different
dilutions of Novidat
Figure No : 04 linearity plot for assay of different
dilutions of Axcin
Figure No : 05 Linearity plot for assay of
different dilutions of Cyrocin
Figure No : 06 Linearity plot for assay of
different dilutions of Cycin
Figure No : 07 Linearity plot for assay of
different dilutions of Quinoflox
Figure No : 08 Linearity plot for assay of
different dilutions of Ciproxin

Table Of Content:
Table No: 01 Different brands of ciprofloxacin
Table No: 02 Assay of different brands of
ciprofloxacin
Table No: 03 Absorbance of different brands
Table No: 04 Regression equations of different
brands
 Conclusion
A good linear relationship was observed in the concentration
ranges of 100ppm, 50ppm, 25ppm, 12.5ppm and 6.25ppm for
different brands of ciprofloxacin tablets i.e. NOVIDAT, AXCIN,
CYROCIN, CYCIN, and QUINOFLOX AND CIPROXIN. The
correlation coefficient for different brands of ciprofloxacin was
found to be 0.9998 for (A), 0.9992 for (B), 0.9996 for (C) and
CYCIN (D), 0.9989 for (E) and 0.9986 for (F) which are within the
limit.

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THE END