Zoology Honours Regular
Zoology Honours Regular
Zoology Honours Regular
for
B.Sc. (Honors)ZOOLOGY
BERHAMPUR UNIVERSITY
BHANJA BIHAR
Page 1 of 54
SCHEME FOR CHOICE BASED CREDIT SYSTEMIN B.Sc. ZOOLOGY HONORS
Semester-I Credits
Semester-IV Credits
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Core Course-8 Biochemistry of Metabolic Processes 4
Semester-V Credits
Semester-VI Credits
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CORE COURSE: ZOOLOGY
PaperI
THEORY
Unit 1:PhylumProtozoa, Parazoa andMetazoa
Generalcharacteristicsandclassificationuptoclasses; Lifecycle,pathogenicityand
prophylaxisofPlasmodiumvivax,TrypanosomagambienseandEntamoebahistolytica;
Locomotion and reproduction in Protozoa, origin of Metazoa.
Unit 3:PhylumCnidaria
Generalcharacteristicsandclassificationuptoclasses;MetagenesisinObelia; Polymorphism
in Cnidaria; Corals andcoral reefs.
Unit 4:PhylumHelminthes
Generalcharacteristicsandclassificationuptoclasses; Lifecycle,pathogenicityand
prophylaxis of Fasciola hepatica and Ascarislumbricoides;Parasiticadaptations.
PRACTICALS
PhylumProtozoa
1. MorphologyofParamecium,Binaryfission andConjugation in Paramecium.
2. LifestagesofPlasmodiumvivax,TrypanosmagambienseandEntamoebahistolytica
(Slides/Micro-photographs).
3. Examination of pond waterfor protists.
PhylumPorifera
4. StudyofSycon(includingT.S. andL.S.),Hyalonema, and Euplectella.
5. Temporarymounts of spicules, gemmulesand sponginfibres.
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PhylumCnidaria
6. StudyofObelia,Physalia,Millepora,Aurelia,Ephyralarva,Tubipora,Corallium,
Alcyonium, Gorgoniaand Metridium(includingT.S.andL.S.).
PhylumCtenophora
7. Anyonespecimen/slide.
PhylumHelminthes
8. Study of adult Fasciolahepatica, Taeniasoliumand their life stages (Slides/
microphotographs).Studyof
adultAscarislumbricoides,Wuchereriabancroftiandtheirlifestages(Slides/
microphotographs).
Note:Classification to be followed from“BarnesRD (1982)InvertebrateZoology. 5thEdition.”
SUGGESTED READINGS
1. AroraMP (2006) Non-Chordata-I. 1stedition. HimalayaPublishing House,New Delhi.
2. AroraMP (2008)Non-Chordata-II.1stedition. HimalayaPublishingHouse,New Delhi.
3. Barnes RD(1982)InvertebrateZoology.6thEdition. Holt SaundersInternational Edition.
4. BarnesRSK,CalowP,OlivePJW,GoldingDW& SpicerJI(2002)TheInvertebrates:A
NewSynthesis. 3rdEdition. Blackwell Science,USA.
5. Barrington EJW(1979)Invertebrate Structure andFunctions. 2ndEdition.ELBS and
Nelson.
6. BoradaleLAand Potts EA (1961)Invertebrates:AManual fortheuseofStudents. Asia
PublishingHome.
7. Jordan ELandVermaPS(1963)InvertebrateZoology.RevisedEdition.S.Chand,New Delhi.
8. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
9. Kotpal R.L Modern Text Book of Zoology, Invertebrates. RastogiPublication,Meerut.
10. Singh.H.S. &Rastogi.P., Parasitology,RastogiPublication,Meerut.
Page 5 of 54
CORE COURSE: ZOOLOGY
PAPER II
Principles and Ethics of Ecology
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 2:Population
Unitaryandmodularpopulations;Uniqueandgroupattributesofpopulation:Density,
natality,mortality,lifetables,fecunditytables,survivorshipcurves,ageratio,sexratio,
dispersalanddispersion;Exponentialandlogisticgrowth,equationandpatterns,randK
strategies,Populationregulation-density-dependentandindependentfactors;Population
interactions,Gause’sPrinciplewithlaboratoryandfieldexamples
Unit3:Community
Communitycharacteristics:dominance,diversity,speciesrichness,abundance,
stratification;Ecotoneandedgeeffect;Ecosystemdevelopment(succession)with exampleand
Theories pertainingto climaxcommunity.
Unit 4:Ecosystem
Typesofecosystem;Foodchain,Detritusandgrazingfoodchains,LinearandY-shaped
foodchains;Foodweb;Energyflowthroughtheecosystem;Ecologicalpyramidsand
Ecologicalefficiencies;Nutrientandbiogeochemicalcycle,NitrogencycleandSulphur cycle.
PRACTICALS
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3. Studyofanaquaticecosystem:faunaandfloraMeasurementofarea,
temperature,turbidity/penetrationoflight,determinationofpH,andDissolvedOxygen
content(Winkler’s method), Chemical OxygenDemandand freeCO2.
SUGGESTED READINGS
1. ColinvauxPA(1993)Ecology.IIEdition. JohnWileyand Sons,Inc., USA.
2. Dash MC(1993)Fundamentals of Ecology. McGraw Hill Book Company,New Delhi.
3. JoshiNandJoshiPC(2012)EcologyandEnvironment.1stEdition.HimalayaPublishing House,
NewDelhi.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. Odum EP (2008)Fundamentals of Ecology.Indian Edition. Brooks/Cole.
6. Ricklefs, R.E., (2000). Ecology. 5thEdition. Chiron Press.
7. RobertLeo Smith EcologyandfieldbiologyHarperand Row.
8. SinghJS, Gupta SR and Singh SP (2014) Ecology, Environmental Science and
Conservation. S. Chand, New Delhi.
9. Sharma P.D (2011) : Ecology and Environment. RastogiPublication,Meerut.
Page 7 of 54
CORE COURSE: ZOOLOGY
PAPER III
Biology of Non-Chordata-II
Coelomate Nonchordates
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:PhylumAnnelida
Generalcharacteristicsandclassificationuptoclasses;EvolutionofCoelom; Metamerism and
Excretion in Annelida.
Unit 3:PhylumMollusca
Generalcharacteristicsandclassificationuptoclasses;RespirationinMollusca;Torsion
inGastropoda, Evolutionarysignificance of trochophorelarva.
Unit 4:PhylumEchinodermata
Generalcharacteristicsandclassificationuptoclasses;Water-vascularsystemin
Asteroidea;LarvalformsinEchinodermataandits Evolutionarysignificance
Note: Classification tobefollowed from “Barnes, R.D. (1982). InvertebrateZoology, 5thEdition,
Holt SaundersInternational Edition.”
PRACTICAL
PhylumAnnelida
1. StudyofAphrodite,Nereis,Heteronereis,Sabella, Terebella,Serpula,Chaetopterus,
Pheretimaand Hirudinaria.
2. T.S. through pharynx, gizzard,and typhlosolarintestineofearthworm.
3. T.S. through cropof leech.
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PhylumArthropoda
4. Study of Limulus, Palamnaeus, Palaemon, Daphnia, Balanus, Sacculina, Cancer,
Eupagurus, Scolopendra, Julus, termite, louse, honeybee, silk moth, waspand dragon fly.
PhylumOnychophora
5. Anyonespecimen/slide.
PhylumMollusca
6. Study of Chiton, Dentalium, Pila, Doris, Helix, Unio, Ostrea, Mytilus, Loligo,
Sepia,Octopusand Nautilus and Cypraea(cowrie).
PhylumEchinodermata
7. Studyof echinodermlarvae.
8. Study of Pentaceros, Asterias, Ophiura, Clypeaster, Echinus, Echinocardium,
Cucumariaand Antedon.
Note: Classification tobe followed from “Barnes, R.D. (1982). InvertebrateZoology, 5thEdition,
Holt SaundersInternational Edition”.
SUGGESTED READINGS
1. AroraMP (2006)Non-Chordata-I. 1stedition. HimalayaPublishingHouse, New Delhi.
2. AroraMP (2008)Non-Chordata-II.1stedition.HimalayaPublishing House, New Delhi.
3. Barnes RD(1982)InvertebrateZoology. 6thEdition. Holt SaundersInternational Edition.
4. BarnesRSK,CalowP,OlivePJW,GoldingDW&SpicerJI(2002)TheInvertebrates:A
NewSynthesis. 3rdEdition. Blackwell Science,USA.
5. Barrington EJW(1979)Invertebrate Structure and Functions. 2ndEdition.ELBSand
Nelson.
6. BoradaleLAand Potts EA (1961)Invertebrates:A Manual for theuseof Students. Asia
PublishingHome.
7. Jordan ELandVermaPS(1963)InvertebrateZoology.RevisedEdition.S.Chand,New Delhi.
8. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
9. Kotpal R.L Modern Text Book of Zoology, Invertebrates. RastogiPublication,Meerut.
Page 9 of 54
CORE COURSE: ZOOLOGY
PAPER IV
PHYSIOLOGY:LIFESUSTAININGSYSTEMS
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:DigestiveSystem
Structuralorganization,histologyandfunctionsofgastrointestinaltractanditsassociated
glands;Mechanicalandchemicaldigestionoffood;Absorptionsofcarbohydrates,lipids,
proteins,water,mineralsandvitamins;Roleofgastrointestinalhormonesonthesecretion and
control of enzymes ofgastrointestinal tract.
PRACTICAL
1. Enumeration of red blood cells usinghaemocytometer.
2. Estimation of haemoglobin usingSahli’shaemoglobinometer.
3. Preparation ofhaeminand haemochromogencrystals.
4. Recordingof blood pressureusingaSphygmomanometer.
5. Examinationofsectionsofmammalianoesophagus,stomach,duodenum,ileum,rectum liver,
trachea, lungand kidney.
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SUGGESTED READINGS
1. AreyLB (1974)HumanHistology. 4thEdition.W.B. Saunders, USA.
2. ChatterjeeCC (2008)Human Physiology.Vol.IandII. MedicalAllied Agency, Kolkata.
3. GuytonAC andHall JE(2006) Textbook of Medical Physiology. 9thEdition. W.B.
Saunders Company, Philadelphia.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. TortoraGJand Derrickson B(2012) Principles ofAnatomy&Physiology.13thEdition
JohnWileyand sons, USA.
6. Victor PE (2008)diFiore’sAtlas of Histologywith Functional Correlations. 12thEdition.
Lippincott W. &Wilkins, USA.
7. Goyal K.A. &Shastri K.V. Animal Physiology, RastogiPublication.Meerut.
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CORE COURSE: ZOOLOGY
PAPER V
BIOLOGY OFCHORDATA
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 4:Mammals
Generalcharactersandclassificationuptoorder;Affinitiesof
PrototheriaandMetatheria;Dentitioninmammals;Adaptiveradiationwithreferencetolocomo
toryappendages;
PRACTICAL
1. Protochordata
1. Balanoglossus, Herdmania, Branchiostomaand Colonial Urochordata.
2. Sections ofBalanoglossusthrough proboscis andbranchiogenitalregions.
3. Sections ofAmphioxus through pharyngeal, intestinal and caudal regions.
4. Permanent slide ofspicules ofHerdmania.
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2.Agnatha
5. Petromyzon and Myxine.
3. Fishes
6. Sphyrna, Pristis, Trygon, Torpedo, Chimaera, Notopterus, Mystus, Heteropneustes,
Hippocampus, Exocoetus, Echeneis, Anguilla, Tetrodon,Diodon, Anabas and Flat fish.
4.Amphibia
7. Ichthyophis/Ureotyphlus, Necturus, Duttaphrynus, Polypedates, Hyla, Alytes and
Salamandra.
5. Reptiles
8. Chelone, Trionyx, Hemidactylus, Varanus, Uromastix, Chamaeleon, Draco,
Ophiosaurus, Bungarus, Vipera, Naja, Hydrophis, Zamenisand Crocodylus.
9. KeyforIdentificationofpoisonous and non-poisonous snakes.
6. Aves
10. Studyof sixcommon birds from different orders.
11. Types of beaksand claws.
12. Types offeathers.
7. Mammalia
13.Sorex, Bat (Insectivorous and Frugivorous), Funambulus, Loris, Herpestesand
Hemiechenis.
SUGGESTED READINGS
1. Agarwal VK (2011)Zoologyfor degreestudents.S. Chand, New Delhi.
2. AroraMP (2006) Chordata-1. 1stEdition. HimalayaPublishingHouse, NewDelhi.
3. Hall BKand Hallgrimsson B(2008)Strickberger’s Evolution. 4thEdition.Jones and
Bartlett PublishersInc.,USA.
4. Jordan ELand VermaPS(1963)ChordateZoology.RevisedEdition.S.Chand,New
Delhi.
5. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
6. YoungJZ(2004)TheLifeof Vertebrates. 3rdEdition. OxfordUniversityPress, USA.
7. Kotpal R.L. (2015) Modern Text Book of Zoology, Vertebrates,
RastogiPublication,Meerut.
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CORE COURSE: ZOOLOGY
PAPER VI
PHYSIOLOGY – CONTROLLINGAND COORDINATINGSYSTEM
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:Tissues and Glands, Boneand cartilage
Structure,location,functionandclassificationofEpithelialtissue,Connectivetissue,
Musculartissue,Nervoustissue;Typesofglandsandtheirfunctions;Structureandtypes of
bones and cartilages;Ossification, bonegrowth and resorption.
Unit 3: Muscle
Histology ofdifferenttypesofmuscle;Ultrastructureofskeletalmuscle;Molecularand
chemicalbasisofmusclecontraction;Characteristicsofmuscletwitch;MotorUnit, summation
and tetanus.
Unit 4:EndocrineSystem
FunctionalHistologyofendocrine glands-pineal,pituitary,thyroid,parathyroid,thymus,
pancreas,adrenals;Hormonessecretedbythemandtheirmechanismofaction;Classificationofh
ormones;Regulationoftheirsecretion;Modeofhormone action
Hypothalamus(neuroendocrinegland),
PRACTICALS
1. Demonstrationoftheunconditionedreflexaction(Deeptendonreflexsuchaskneejerk reflex).
2. Preparation oftemporarymounts: Squamousepithelium, Striatedmusclefibresandnerve
cells.
3. Examinationofsectionsofmammalianskin,Cartilage,Bone,Spinalcord,Nervecell, Pituitary,
Pancreas, Testis, Ovary, Adrenal, Thyroid and Parathyroid.
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SUGGESTED BOOKS
1. AreyLB (1974)HumanHistology. 4thEdition.W.B. Saunders, USA.
2. ChatterjeeCC (2008)Human Physiology.Vol.IandII. MedicalAllied Agency, Kolkata.
3. GuytonAC andHall JE(2006) Textbook of Medical Physiology. 9thEdition. W.B.
Saunders Company, Philadelphia.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. TortoraGJand Derrickson B(2012) Principles ofAnatomy&Physiology.13thEdition
JohnWileyand sons, USA.
6. Victor PE (2008)diFiore’sAtlas of Histologywith Functional Correlations. 12thEdition.
Lippincott W. and Wilkins, USA.
7. Goyal K.A. &Shastri K.V. Animal Physiology, RastogiPublication.Meerut.
8. Pocock G.&Rechards C. The Human Body, Oxfort University Press,U.K.
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CORE COURSE: ZOOLOGY
PAPER VII
COMPARATIVE ANATOMYOFVERTEBRATES
(CREDITS:THEORY-4, PRACTICALS-2)
THEORY
PRACTICAL
1. Studyof placoid,cycloidand ctenoid scales through permanent slides/photographs.
2. Disarticulated skeleton of Frog, Varanus,Fowl and Rabbit.
3. Carapace and plastron ofturtleor tortoise.
4. Mammalian skulls(Oneherbivorous and one carnivorous animal).
SUGGESTED READINGS
1. Hilderbrand Mand Gaslow GE. Analysis of Vertebrate Structure. John Wileyand Sons.,
USA.
2. KardongKV(2005) Vertebrates’Comparative Anatomy,Function and Evolution.
4thEdition. McGraw-Hill Higher Education, New York.
3. Kent GC and CarrRK (2000)Comparative Anatomyof theVertebrates. 9thEdition. The
McGraw-Hill Companies, New York.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. Weichert CK and William Presch (1970) Elements of Chordate Anatomy.TataMcGraw
Hill, New York.
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CORE COURSE: ZOOLOGY
PAPER VIII
BIOCHEMISTRYOFMETABOLIC PROCESSES
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 3:ProteinMetabolism
Catabolismofaminoacids:Transamination,Deamination;Ureacycle;FateofC-skeleton
ofGlucogenic andKetogenicamino acids.
PRACTICALS
1. Identificationofunknowncarbohydratesingivensolutions(Starch,Sucrose,Lactose,
Galactose, Glucose, Fructose).
2. Colour tests offunctionalgroups in protein solutions.
3. Action ofsalivaryamylaseunder optimum conditions.
4. Effect of pHon theaction ofsalivaryamylase.
5. Effect of temperatureontheaction ofsalivaryamylase.
6. Estimation of total protein in given solutionsbyLowry’s method.
SUGGESTED READINGS
1. BergJM, TymoczkoJLand StryerL(2007)Biochemistry. 6thEdition, W.H. Freeman and
Co., New York.
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2. CoxMM and Nelson DL(2008)Lehninger Principles of Biochemistry. 5thEdition. W.H.
Freeman and Co.,NewYork.
3. DevesenaT (2014) Enzymology.2ndEdition. OxfordUniversityPress, UK.
4. HamesBD and HooperNM (2000)Instant Notesin Biochemistry. 2ndEdition. BIOS
ScientificPublishersLtd., U.K.
5. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
6. MurrayRK,BenderDA,Botham KM, KennellyPJ,RodwellVWand WellPA(2009)
Harper’sIllustratedBiochemistry. 28thEdition.International Edition. TheMcGraw-Hill
CompaniesInc.,New York.
7. Trudy Mckee, & James Mckee5thEdition,Biochemistry, Oxpord University Press,U.K.
8. Gupta S.N., A Text Book of Bio-Chemistry, RastogiPublication,Meerut.
Page 18 of 54
CORE COURSE: ZOOLOGY
PAPER IX
CELL BIOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit4:Apoptosis andCancer
Extrinsic(DeathReceptor)PathwayandIntrinsic(Mitochondrial)Pathway;Growthand
development of tumors and Metastasis.
PRACTICAL
1. Gram’s stainingtechniqueforvisualization ofprokaryotic cells.
2. Studyvarious stages ofmitosis from permanent slides.
3. Studyvarious stages ofmeiosis from permanentslides.
4. StudythepresenceofBarrbodyinhumanfemalebloodcells/cheekcells.(Preparationof
permanent slides).
5. Cytochemical demonstration (Preparation ofpermanent slides).
i. DNAbyFeulgen reaction.
ii. MucopolysaccharidesbyPAS reaction.
iii. ProteinsbyMercurobromophenol blue.
iv. DNAand RNAbyMethylGreenPyronin.
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(In practical examination, 05 marks should be of permanent slide submission; one
markeachfor DNA,PAS, Proteins, MGPandBarrbody slide.)
SUGGESTED READINGS
1. Becker WM, KleinsmithLJ,Hardin Jand Bertoni G P (2009)TheWorld ofthe Cell.
7thEdition. Pearson Benjamin CummingsPublishing,San Francisco.
2. BruceAlbert, BrayDennis,Levis Julian, RaffMartin, Roberts Keith and Watson James
(2008)MolecularBiologyof theCell. 5thEdition.Garland publishingInc.,New York.
3. CooperGM and Hausman RE (2009)TheCell: AMolecularApproach. 5thEdition. ASM
Press, Washington D.C.
4. DeRobertis EDP and DeRobertis EMF(2006)Cell and MolecularBiology. 8thEdition.
Lippincott Williams andWilkins,Philadelphia.
5. Karp G(2010)Cell and Molecular Biology: Concepts and Experiments. 6thEdition.John
Wileyand Sons.Inc.,USA.
6. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
7. Debasena.T. Cell Biology,Oxford University Press,U.K.
8. Gupta.P.K., Cell & Molecular Biology, RastogiPublication,Meerut.
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CORE COURSE: ZOOLOGY
PAPER X
PRINCIPLES OF GENETICS
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:Mendelian Genetics andits Extension
Principlesofinheritance;Incompletedominanceandco-dominance;Multiplealleles,
Lethalalleles; Epistasis;Pleiotropy; Sex-linked inheritance.
Unit 3:Mutations
Genemutations;Chromosomalmutations:Deletion,duplication,inversion,
translocation;Aneuploidyandpolyploidy;Inducedversusspontaneousmutations;
Backwardandforwardmutations;Suppressormutations;Molecularbasisofmutationsin
relationtoUVlightandchemicalmutagens;Detectionofmutations:CLBmethod, attached
Xmethodand DNA repair mechanisms.
PRACTICAL
1. TostudytheMendelianlawsandgeneinteractionsandtheirverificationbyChisquareanalyses
usingseeds/beads/Drosophila.
2. Identification ofvariousmutants of Drosophila.
3. Tocalculate allelic frequenciesbyHardy-WeinbergLaw.
4. Linkagemaps based on datafrom crosses ofDrosophila.
5. Studyof human karyotype(normal andabnormal).
6. Pedigree analysis of somehuman inherited traits.
7. Preparation ofpolytenechromosomes from larvaofChironomous/Drosophila.
8. To studyMutagenicityinSalmonella/E. colibyAmes test.
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SUGGESTED READINGS
1. Gardner EJ, SimmonsMJ,Snustad DP (2008)Principles of Genetics. 8thEdition. Wiley
India.
2. Griffiths AJF, WesslerSR,Lewontin RC and Carroll SB.Introduction toGenetic
Analysis. 9thEdition. W.H. Freeman and Co.,New York.
3. KlugWS, CummingsMR, Spencer CA and Palladino MA (2012)Concepts of Genetics.
10thEdition. Pearson Edsucation,Inc., USA.
4. MohantyPK(2000)Illustrated DictionaryofBiology.Kalyani Publishers,Ludhiana.
5. Russell PJ(2009) Genetics-A MolecularApproach. 3rdEdition. BenjaminCummings,
USA.
6. Snustad DP and Simmons MJ(2012)Principles of Genetics. 6thEdition.John Wileyand
SonsInc., USA.
7. VermaPS and AgarwalVK(2010) Genetics. 9thEdition. S. Chand, New Delhi.
Page 22 of 54
CORE COURSE: ZOOLOGY
PAPER XI
DEVELOPMENTALBIOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:Early Embryonic Development
Gametogenesis(Spermatogenesis,Oogenesis);Typesofeggs;Eggmembranes;
Fertilization:Changesingametes,monospermyandpolyspermy;Planesandpatternsof
cleavage;earlydevelopmentoffrogandchickuptogastrulation;Fatemaps;embryonic
induction andorganizers.
Unit 2:LateEmbryonicDevelopment
Fateofgermlayers;Extra-embryonicmembranesinbirds;Implantationofembryoin
humansand Placenta(Structure, types andfunctions of placenta).
Unit 3:PostEmbryonicDevelopment
Metamorphosis:Changes,hormonalregulationsinamphibians;Regeneration:Modesof
regeneration(epimorphosis,morphallaxisandcompensatoryregeneration);Ageing: Concepts
and models.
PRACTICAL
1. Studyofwholemountsandsectionsofdevelopmentalstagesoffrogthroughpermanent
slides:Cleavagestages,blastula,gastrula,neurula,tail-budstage,tadpole(externaland internal
gill stages).
2. Studyofwholemountsofdevelopmentalstagesofchickthroughpermanent
slides:Primitivestreak(13and18hours),21,24,28,33,36,48,72,and96hoursof
incubation(HamiltonandHamburger stages).
3. Studyofdevelopmentalstages(abovementioned)byraisingchickembryointhe laboratory.
4. Studyof thedevelopmental stages and lifecycleofDrosophila from stock culture.
5. Studyof differenttypes ofplacenta.
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6. Project report onDrosophila culture/chick embryo development.
SUGGESTED READINGS
1. BalinskyBIandFabianBC (1981)AnIntroduction to Embryology.5thEdition.
International ThompsonComputerPress.
2. Gilbert SF(2010)Developmental Biology. 9thEdition. SinauerAssociates,Inc.,USA.
3. Kalthoff(2008) Analysis of Biological Development. 2ndEdition. McGraw-Hill, New
York.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. WolpertL, BeddingtonR,Jessell T, LawrenceP,MeyerowitzEand Smith J(2002)
Principles of Development. 1stEdition, Oxford UniversityPress,NewYork.
6. Sastry K.V & Shukla Vinita, Developmental Biology, RastogiPublication,Meerut.
Page 24 of 54
CORE COURSE: ZOOLOGY
PAPER XII
MOLECULARBIOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
PRACTICAL
1. StudyofDNAreplicationusingPhotographsorslidesandspecialcases,e.g.,Polyteny
usingpermanent slides of polytene chromosomes.
2. Preparation ofliquid culturemedium (LB)andraise cultureofE. coli.
3. Estimation of the growthkinetics ofE.coli byturbiditymethod.
Page 25 of 54
4. Preparation of solid culture medium (LB) and growth of E. coli byspreading and
streaking.
5. Demonstrationofantibioticsensitivity/resistanceofE.colitoantibioticpressureand
interpretation of results.
6. Quantitative estimation of salmon sperm/calf thymus DNA using
colorimeter(Diphenylamine reagent) orspectrophotometer(A260 measurement).
7. Quantitativeestimation of RNA usingOrcinol reaction.
SUGGESTED READINGS
1. Becker WM, KleinsmithLJ,Hardin Jand Bertoni GP (2009)TheWorld ofthe Cell.
7thEdition. Pearson Benjamin CummingsPublishing,San Francisco.
2. BruceAlberts, AlexanderJohnson,Julian Lewis,Martin Raff, Keith Roberts, Peter
Walter(2008)MolecularBiologyof theCell, 4thEdition. Garland publishingInc.,New
York.
3. CooperGM and Hausman RE (2007)TheCell: AMolecularApproach. 4thEdition, ASM
Press, USA.
4. DeRobertis EDP and DeRobertis EMF(2006)Cell and MolecularBiology. 8thEdition.
Lippincott Williams andWilkins,Philadelphia.
5. Karp G(2010)Cell and Molecular Biology: Concepts and Experiments. 6thEdition.John
Wileyand Sons.Inc., USA.
6. MohantyPK(2000)Illustrated DictionaryofBiology. KalyaniPublishers,Ludhiana.
7. Craig N. & Cohen-fix.O., Molecular Biology, Oxford UniversityPress,U.K.
8. Pal.J.K. &Ghaskadbi S.S., Fundamentals Of MolecularBiology,OxfortUniversity,U.K.
Page 26 of 54
CORE COURSE:
ZOOLOGYPAPER XIII
IMMUNOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 2:Antigens
Antigenicity andimmunogenicity,Immunogens,Adjuvantsandhaptens,Factors
influencingimmunogenicity, Band T-Cell epitopes.
Unit 3: Immunoglobulins
Antibody structure and function, antibody isotypes, Applications Monoclonalantibodies.
Unit4:Hypersensitivity
Type-I hypersensitivity- allergens, mast cell degranulation, mediators of type-I reaction,
Type-II-antibody mediated cytotoxic, Type-III and Type IV hypersensitivity.
PRACTICAL
1. Demonstration of lymphoid organs.
2. Ouchterlony’s doubleimmuno-diffusion method.
3. Determination of ABOblood group.
4. Preparationofsinglecellsuspensionofsplenocytesfromchickspleen,cellcountingand
viabilitytest.
5. ELISA/ dot Elisa (usingkit).
6. Principles, experimental set up andapplications of immuno-electrophoresis,RIA,F.
Page 27 of 54
SUGGESTED READINGS
Page 28 of 54
CORE COURSE: ZOOLOGY
PAPER XIV
EVOLUTIONARYBIOLOGY
(CREDITS:THEORY-4, PRACTICALS-2)
THEORY
Unit 1:History ofLife,theories ofEvolution and Extinction
Chemogeny,Biogeny,RNAWorld,MajorEventsinHistoryofLife;Lamarckism;
Darwinism;Neo-Darwinism;Backgroundofextinction,Massextinction(Causes,Names
offive major extinctions, K-T extinction in detail) and Role ofextinction inevolution.
PRACTICAL
1. Studyoffossil evidencesfrom plaster cast models and pictures.
2. Studyof homologyandanalogyfrom suitable specimens/ pictures.
3. Demonstration of changingallele frequencieswith and without selection.
4. Construction of cladogram based on morphological characteristics.
5. Construction ofphylogenetictreewith bioinformatics tools (Clustal X and Phylip).
6. Interpretation ofphylogenetictrees.
Page 29 of 54
SUGGESTED READINGS
1. Barton NH,Briggs DEG,EisenJA, Goldstein DBand Patel NH(2007)Evolution. Cold
SpringHarbourLaboratoryPress.
2. Campbell NAand ReeceJB(2011)Biology. 9thEdition. Pearson EducationInc., New
York.
3. Douglas JF(1997) EvolutionaryBiology. SinauerAssociates, USA.
4. Hall BKand Hallgrimsson B(2008)Evolution. 4thEdition.Jones and Bartlett Publishers,
USA.
5. MohantyPK(2000)Illustrated DictionaryofBiology.Kalyani Publishers,Ludhiana.
6. Pevsner J(2009) BioinformaticsandFunctional Genomics. 2ndEdition. Wiley-Blackwell,
USA.
7. RidleyM (2004) Evolution. 3rdEdition. Blackwell Publishing, USA.
8. Tomar B.S & Singh S.P. Evolutionary Biology,RastogiPublication,Meerut.
Page 30 of 54
DISCIPLINE SPECIFIC ELECTIVE-I
ANIMAL BEHAVIOUR
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
PRACTICAL
1. Tostudydifferenttypesofanimalbehavioursuchashabituation,sociallife,courtship
behaviourininsects,andparentalcarefromshortvideos/moviesandprepareashort report.
2. To studynests and nestinghabits of thebirds andsocial insects.
3. To studythe behaviouralresponses ofwoodlicetodrycondition.
4. To studybehaviouralresponses ofwood licein response to humid condition.
5. To studygeotaxis behaviour inearthworm.
6. To studythephototaxisbehaviour in insect larvae.
7. Visit to Forest/ Wild life Sanctuary/Biodiversity Park/Zoological Park to study
Page 31 of 54
behaviouralactivities of animals and prepareashort report.
SUGGESTED READINGS
1. David McF. Animal Behaviour. Pitman PublishingLimited,London, UK.
2. John A(2001)Animal Behaviour. 7thEdition.SinauerAssociateInc., USA.
3. ManningA and Dawkins MS. AnIntroduction toAnimal Behaviour. Cambridge
UniversityPress, USA.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. Paul WS andJohn A (2013)ExploringAnimal Behaviour. 6thEdition.SinauerAssociate
Inc., Massachusetts, USA.
6. Mathur R. Animal Behaviour, RastogiPublication,Meerut.
Page 32 of 54
DISCIPLINE SPECIFIC ELECTIVE II
ANIMAL BIOTECHNOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 1:Concept and scope of Biotechnology, Cloning vectors (Types and characteristics),
Restriction enzymes: Nomenclature, detailed study of Type-II.
Unit 2:Transformation techniques: Calcium chloride method and electroporation, Construction of
genomic and cDNA libraries and screening, Bloting techniques, DNA sequencing,
Polymerase Chain Reaction, DNA finger printing and DNA microarray
Unit3:Production of cloned and transgenic animals: Nuclear transplantation, retroviral method,
DNA microinjection, Applications of Transgenic animals: production of pharmaceuticals,
knock out mice, donor organs, production of transgenic plants: Agrobacterium mediated
transformation, Application of Transgenic plants: insect and herbicide resistant plants.
Unit4:Animal cell culture, Expression of cloned genes, molecular diagnosis of genetic diseases
(Cystic fibrosis/Sickle cell anemia), Recombinant DNA in medicine: insulin/growth
hormone, Gene therapy.
PRACTICAL
1. Genomic DNA isolation from E. Coli.
2. Plasmid DNA isolation.
3. Restriction digestion
4. Construction of circular and linear restriction map
5. Calculation of transformation efficiency from the data provided
6. To study the following technique through photographs
Southern Bloting
NothernBloting
DNA sequencing
PCR
DNA fingerprinting
7. Project report on animal cell culture
Page 33 of 54
SUGGESTED READINGS
1. Brown, T. A. Molecular Biology, LabfaxII: Gene cloning and DNA Analysis, II Edition
2. Glick, B. R. and Pasternak, JJ, Molecular Biotechnology-Principles and Application of Recombinant DNA, IV
Edition
3. Griffiths, AJF etal. (2009): An Introduction to Genetic Analysis, IX Edition
4. Snustad, DP and Simmons MJ, Principles of Genetics, V Edition
5. Watson JD, Myers RM, Caudy A and Witkowski JK (2007): Recombinant DNA-Genes and Genomes-A short
Course, III Edition
6. Beauchamp TI and Childress JF (2008): Principles of Biomedical Ethics, VI Edition
Page 34 of 54
DISCIPLINE SPECIFIC ELECTIVE III
MICROBIOLOGY
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
PRACTICAL
1. Cleaningofglasswares,sterilisationprincipleandmethods-moistheat-dryheatand filtration
methods.
2. Mediapreparation:Liquidmedia,Solidmedia,Agarslants,Agarplates.Basal,enriched,
selectivemediapreparation-qualitycontrolofmedia,growthsupportingproperties,
sterilitycheck of media.
3. Pureculturetechniques:Streak plate, pourplateand decimal dilution.
4. Culturalcharacteristicsofmicroorganisms:Growthondifferentmedia,growth characteristics
and description and demonstration ofpigment production.
5. Stainingtechniques:Smearpreparation,simplestaining,Gram’sstaining,Acidfast stainingand
stainingformetachromaticgranules.
6. Morphologyof microorganisms.
7. Antibioticsensitivitytesting: Discdiffusion test- Qualitycontrol with standard strains
8. Physiology characteristics:IMViCtest,H2S,Oxidase,catalase,ureasetest,Carbohydrate
fermentation,Maintenanceofpureculture,Paraffinmethod,Stabcultureand maintenanceof
mold culture.
SUGGESTED READINGS
1. Ahsan Jand Sinha SP(2010)A Hand book on EconomicZoology. S Chand, NewDelhi.
2. AroraDRandAroraB(2001)MedicalParasitology.2ndEdition.CBSPublicationsand
Distributers.
3. AtwalAS(1993)AgriculturalPestsofIndiaandSouthEastAsia.KalyaniPublishers,
Page 35 of 54
Ludhiana.
4. DubeyRCand MaheshwariDK(2013)ATextbookofMicrobiology.S.Chand,New Delhi.
5. Dunham RA(2004) Aquaculture and Fisheries Biotechnology Genetic Approaches.
CABIpublications.
6. PelczarMJ,ChanECSandKriegNR(1993)Microbiology.5thEdition,TataMcGraw Hill
PublishingCo.Ltd.
7. Pradhan, S (1983)InsectPests of Crops. National Book Trust ofIndia, New Delhi.
8. Shrma P.D. Microbiology, RastogiPublication,Meerut.
Page 36 of 54
GENERIC ELECTIVE -I
ANIMAL DIVERSITY
(THEORY-4, PRACTICALS-2)
THEORY
Unit 1:
General characters of Protozoa; Life cycle of plasmodium
General characters & canal system in Porifera
General characters of Cnidarians & Polymorphism in hydrozoa
General characters of Helminthes ; Life cycle of Taeniasolium
General characters ofNemathelminthes ; Parasitic adaptation
Unit 2:
General characters &Metamerismin Annelida ;
General characters of Arthropoda ; Social life in insects
General characters of Mollusca ; Pearl formation
General characters of Echinoderms ; Water vascular system in Starfish
Unit 3:
Salient features of Protochordates
Osmoregulation , Migration of Fishes
General characters of Amphibians ; Adaptation of Amphibians for terrestrial life
Parental care in Amphibia
Unit 4:
Origin of Reptiles ; Terrestrial adaptations in Reptiles
Origin of Birds ; Flight adaptation in birds
Early evolution of Mammals ; Primates ; Dentition in Mammal
PRACTICALS
Page 37 of 54
Salamander,Rhacophorus,Draco,Uromastix,Naja,Viper,Model of Archaeopteryx,
Crow,Duck,Owl,Squirrel,Bat
3. Temporary mount of
Septal & Pharyngeal nephridia of Earthworm
Placoid, cycloid,ctenoid scales
4. Dissection of
Digestive & Nervous system of Prawn,Cockroach.
Urinogenital system of Rat, Calotes.
SUGGESTED BOOKS
1. Barnes,R.D.(1992). Invertebrate Zoology. Saunders college Pub. USA.
2. Ruppert, Fox & Barnes (2006) Invertebrate Zoology.A functional Evolutionary
Approach 7th Edition, Thomson Books/Cole
3. Campbell & Reece (2005). Biology,Pearson Education, (Singapore) Pvt. Ltd.
4. Kardong,K.V.(2002). Vertebrates Comparative Anatomy , Function and Evolution.
Tata MeGraw Hill Publishing Company, New Delhi .
5. Raven, P.H. and Johnson, G.B., (2004). Biology, 6th edition, Tata MeGraw Hill
Publications.New Delhi.
6. Arora MP (2006) Non-Chordata-I&II,1st edition. Himalaya Publishing House, New Delhi.
7. Arors MP (2006) Chordata- I.1st edition. Himalaya Publishing House, New Delhi.
8. Jordan EL AndVerma PS (1963) Invertebrate Zoology. Revised Edition, S.Chand, New Delhi.
9. Jordan EL And Verma PS (1963) Chordate Zoology. Revised Edition, S.Chand, New Delhi.
10. Agarwal VK (2011) Zoology For Degree students. S.Chand, New Delhi.
11. Kotpal R.L Modern Text Book of Zoology, Invertebrates. RastogiPublication,Meerut.
12. Kotpal R.L. (2015) Modern Text Book of Zoology, Vertebrates, RastogiPublication,Meer
Page 38 of 54
GENERIC ELECTIVE – II
ENVIRONMENT AND PUBLIC HEALTH
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 3:AquacultureI
Broodstockmanagement;Inducedbreedingoffish;Managementofhatcheryoffish;
Managementofnursery,rearingandstockingponds;Preparationandmaintenanceoffish
aquarium;Preparationofcompounddietsforfish;Roleofwaterqualityinaquaculture;
Fishdiseases:Bacterial,viralandparasitic;Preservationandprocessingofharvestedfish;
Fisheryby-products.
Unit 4:DairyandPoultryFarming
Introduction;Indigenousandexoticbreeds;Rearing,housing,feedandrationing;
Commercialimportanceofdairyandpoultryfarming;Varietalimprovementtechniques;
Diseasesandtheirmanagement;Dairyorpoultryfarmmanagementandbusinessplan; Visit to
anydairyfarm orPoultryfarm.
Page 39 of 54
* Submission of report on anyone field visits mentioned above.
Page 40 of 54
PRACTICAL
1. Studyof differenttypes ofbees (Queens,Dronesand Worker bees).
2. Studyof differenttypes of silk moths.
3. Studyof differenttypes of pearls.
4. Studyof differenttypes offish diseases.
5. Identification ofdifferent types ofscales in fishes.
6. Studyof differenttypes offins.
7. Studyofdifferentmodifiedstructuresoffishes(Sawofsawfish,Hammerofhammer head fish,
tail of sharksetc.)
8. Identification ofvarious types of natural silks.
SUGGESTED READINGS
1.Dhyan Singh Bisht,Apiculture,ICAR Publication.
2.Dunham RA(2004)Aquacultureand Fisheries Biotechnology–GeneticApproaches.
CABIpublications, U.K.
3.HafezESE (1962) Reproduction in Farm Animals.Lea and Fabiger Publishers.
4.Knobil E and NeillJD(2006)ThePhysiologyofReproduction.Vol. 2. Elsevier
Publishers, USA.
5.MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
6.Prost PJ(1962)Apiculture. Oxford andIBH,NewDelhi.
7.Singh S. BeekeepinginIndia,Indian council ofAgricultural Research,New Delhi.
8.SrivastavaCBL(1999) FisheryScience andIndian Fisheries. KitabMahalpublications,
India.
9.Shukla G.S &UpadhyayV.B(2013) , Economic Zoology, 5thEdition,Rastogi publication,
Meerut.
Page 41 of 54
SYLLABUS
for
BERHAMPUR UNIVERSITY
BHANJA BIHAR
Page 42 of 54
B.Sc Zoology (Regular) under CBCS with effect from the Academic Year 2016/17
Semester-I Credits
Practical A-1 2
Practical A-2 2
Practical A-3 2
Semester-IV Credits
Practical A-4 2
Page 43 of 54
Core C-4 <From Other Subject> 6
Semester-V Credits
Practical DSE-A1 2
Semester-VI Credits
DSE-A2 Biotechnology 4
Practical DSE-A2 2
Page 44 of 54
CORE COURSE: A1
Biology of Non-Chordata
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
PRACTICALS
Phylum Protozoa
9. MorphologyofParamecium,Binaryfission andConjugation in Paramecium.
10. LifestagesofPlasmodiumvivax,TrypanosmagambienseandEntamoebahistolytica
(Slides/Micro-photographs).
11. Examination of pond waterfor protists.
PhylumPorifera
12. StudyofSycon(includingT.S. andL.S.),Hyalonema, and Euplectella.
13. Temporarymounts of spicules, gemmulesand sponginfibres.
Page 45 of 54
PhylumCnidaria
14. StudyofObelia,Physalia,Millepora,Aurelia,Ephyralarva,Tubipora,Corallium,
Alcyonium, Gorgoniaand Metridium(includingT.S.andL.S.).
PhylumCtenophora
15. Anyonespecimen/slide.
Phylum Helminthes
16. Study of adult Fasciolahepatica, Taeniasoliumand their life stages (Slides/
microphotographs).Studyof
adultAscarislumbricoides,Wuchereriabancroftiandtheirlifestages(Slides/
microphotographs).
PhylumAnnelida
17. StudyofAphrodite,Nereis,Heteronereis,Sabella, Terebella,Serpula,Chaetopterus,
Pheretimaand Hirudinaria.T.S. through pharynx, gizzard,and typhlosolarintestineofearthworm.T.S.
through cropof leech
PhylumArthropoda
18. Study of Limulus, Palamnaeus, Palaemon, Daphnia, Balanus, Sacculina, Cancer,
Eupagurus, Scolopendra, Julus, termite, louse, honeybee, silk moth, waspand dragon fly.
PhylumOnychophora
19. Anyonespecimen/slide.
PhylumMollusca
12. Study of Chiton, Dentalium, Pila, Doris, Helix, Unio, Ostrea, Mytilus, Loligo,
Sepia,Octopusand Nautilus and Cypraea(cowrie).
PhylumEchinodermata
13 Studyof echinodermlarvae.
14 Study of Pentaceros, Asterias, Ophiura, Clypeaster, Echinus, Echinocardium,
. Cucumariaand Antedon.
SUGGESTED READINGS
11. AroraMP (2006) Non-Chordata-I. 1stedition. HimalayaPublishing House,New Delhi.
12. AroraMP (2008)Non-Chordata-II.1stedition. HimalayaPublishingHouse,New Delhi.
Page 46 of 54
13. Barnes RD(1982)InvertebrateZoology.6thEdition. Holt SaundersInternational Edition.
14. BarnesRSK,CalowP,OlivePJW,GoldingDW& SpicerJI(2002)TheInvertebrates:A
NewSynthesis. 3rdEdition. Blackwell Science,USA.
15. Barrington EJW(1979)Invertebrate Structure andFunctions. 2ndEdition.ELBS and
Nelson.
16. BoradaleLAand Potts EA (1961)Invertebrates:AManual fortheuseofStudents. Asia
PublishingHome.
17. Jordan ELandVermaPS(1963)InvertebrateZoology.RevisedEdition.S.Chand,New Delhi.
18. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
19. Kotpal R.L Modern Text Book of Zoology, Invertebrates. RastogiPublication,Meerut.
20. Singh.H.S. &Rastogi.P., Parasitology,RastogiPublication,Meerut.
Page 47 of 54
CORE COURSE: A2
Biology of Chordata
(CREDITS: THEORY-4, PRACTICALS-2)
THEORY
Unit 4:Mammals
Generalcharactersandclassificationuptoorder;Affinitiesof
PrototheriaandMetatheria;Dentitioninmammals;Adaptiveradiationwithreferencetolocomo
toryappendages;
PRACTICAL
2. Protochordata
1. Balanoglossus, Herdmania, Branchiostomaand Colonial Urochordata.
2. Sections ofBalanoglossusthrough proboscis andbranchiogenitalregions.
3. Sections ofAmphioxus through pharyngeal, intestinal and caudal regions.
4. Permanent slide ofspicules ofHerdmania.
Page 48 of 54
2.Agnatha
5. Petromyzon and Myxine.
3. Fishes
6. Sphyrna, Pristis, Trygon, Torpedo, Chimaera, Notopterus, Mystus, Heteropneustes,
Hippocampus, Exocoetus, Echeneis, Anguilla, Tetrodon,Diodon, Anabas and Flat fish.
4.Amphibia
7. Ichthyophis/Ureotyphlus, Necturus, Duttaphrynus, Polypedates, Hyla, Alytes and
Salamandra.
8. Reptiles
8. Chelone, Trionyx, Hemidactylus, Varanus, Uromastix, Chamaeleon, Draco,
Ophiosaurus, Bungarus, Vipera, Naja, Hydrophis, Zamenisand Crocodylus.
9. KeyforIdentificationofpoisonous and non-poisonous snakes.
9. Aves
10. Studyof sixcommon birds from different orders.
11. Types of beaksand claws.
12. Types offeathers.
10. Mammalia
13.Sorex, Bat (Insectivorous and Frugivorous), Funambulus, Loris, Herpestesand
Hemiechenis.
SUGGESTED READINGS
8. Agarwal VK (2011)Zoologyfor degreestudents.S. Chand, New Delhi.
9. AroraMP (2006) Chordata-1. 1stEdition. HimalayaPublishingHouse, NewDelhi.
10. Hall BKand Hallgrimsson B(2008)Strickberger’s Evolution. 4thEdition.Jones and
Bartlett PublishersInc.,USA.
11. Jordan ELand VermaPS(1963)ChordateZoology.RevisedEdition.S.Chand,New
Delhi.
12. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
13. YoungJZ(2004)TheLifeof Vertebrates. 3rdEdition. OxfordUniversityPress, USA.
14. Kotpal R.L. (2015) Modern Text Book of Zoology, Vertebrates,
RastogiPublication,Meerut.
Page 49 of 54
CORE COURSE: A3
Cytogenetics and Molecular Biology
(CREDITS: THEORY-4, PRACTICALS-2)
.
THEORY
Unit 1: Cytology
Prokaryotic andEukaryotic cells;Plasma Membrane and Fluid Mosaic Model Transport
across cell membrane, Cell cycle, Endomembranes:
TheEndoplasmicReticulum;Golgiapparatus;Lysosomes;Peroxisomes
PRACTICAL
Page 50 of 54
8. StudyofDNAreplicationusingPhotographsorslidesandspecialcases,e.g.,Polyteny usingpermanent slides
of polytene chromosomes.
9. Preparation ofliquid culturemedium (LB)andraise cultureofE. coli.
10. Estimation of the growthkinetics ofE.coli byturbiditymethod.
9. TostudytheMendelianlawsandgeneinteractionsandtheirverificationbyChisquareanalyses
usingseeds/beads/Drosophila.
10. Identification ofvariousmutants of Drosophila.
11. Tocalculate allelic frequenciesbyHardy-WeinbergLaw.
12. Linkagemaps based on datafrom crosses ofDrosophila.
13. Studyof human karyotype(normal andabnormal).
14. Pedigree analysis of somehuman inherited traits.
15. Preparation ofpolytenechromosomes from larvaofChironomous/Drosophila.
16. To studyMutagenicityinSalmonella/E. colibyAmes test.
SUGGESTED READINGS
1. Becker WM, KleinsmithLJ,Hardin Jand Bertoni GP (2009)TheWorld ofthe Cell. 7thEdition. Pearson
Benjamin CummingsPublishing,San Francisco.
2. BruceAlberts, AlexanderJohnson,Julian Lewis,Martin Raff, Keith Roberts, Peter
Walter(2008)MolecularBiologyof theCell, 4thEdition. Garland publishingInc.,New York.
3. CooperGM and Hausman RE (2007)TheCell: AMolecularApproach. 4thEdition, ASM Press, USA.
4. DeRobertis EDP and DeRobertis EMF(2006)Cell and MolecularBiology. 8thEdition.
Lippincott Williams andWilkins,Philadelphia.
5. Karp G(2010)Cell and Molecular Biology: Concepts and Experiments. 6thEdition.John Wileyand
Sons.Inc., USA.
6. MohantyPK(2000)Illustrated DictionaryofBiology. KalyaniPublishers,Ludhiana.
7. Craig N. & Cohen-fix.O., Molecular Biology, Oxford UniversityPress,U.K.
8. Pal.J.K. &Ghaskadbi S.S., Fundamentals Of MolecularBiology,OxfortUniversity,U.K.
9. Becker WM, KleinsmithLJ,Hardin Jand Bertoni G P (2009):theWorld ofthe Cell. 7thEdition. Pearson
Benjamin CummingsPublishing,San Francisco.
10. BruceAlbert, BrayDennis,Levis Julian, RaffMartin, Roberts Keith and Watson James
(2008)MolecularBiologyof theCell. 5thEdition.Garland publishingInc.,New York.
Page 51 of 54
11. CooperGM and Hausman RE (2009)TheCell: AMolecularApproach. 5thEdition. ASM Press,
Washington D.C.
12. DeRobertis EDP and DeRobertis EMF(2006)Cell and MolecularBiology. 8thEdition.
Lippincott Williams andWilkins,Philadelphia.
13. Karp G(2010)Cell and Molecular Biology: Concepts and Experiments. 6thEdition.John Wileyand
Sons.Inc.,USA.
14. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
15. Debasena.T. Cell Biology,Oxford University Press,U.K.
16. Gupta.P.K., Cell & Molecular Biology, RastogiPublication,Meerut.
Unit 2:LateEmbryonicDevelopment
Fateofgermlayers;Extra-embryonicmembranesinbirds;Implantationofembryoin humansand
Placenta(Structure, types andfunctions of placenta).
Unit 3:PostEmbryonicDevelopment
Metamorphosis:Changes,hormonalregulationsinamphibians;Regeneration:Modesof
regeneration(epimorphosis,morphallaxisandcompensatoryregeneration);Ageing: Concepts and models.
PRACTICAL
1. Studyofwholemountsandsectionsofdevelopmentalstagesoffrogthroughpermanent
slides:Cleavagestages,blastula,gastrula,neurula,tail-budstage,tadpole(externaland internal gill stages).
2. Studyofwholemountsofdevelopmentalstagesofchickthroughpermanent
slides:Primitivestreak(13and18hours),21,24,28,33,36,48,72,and96hoursof
incubation(HamiltonandHamburger stages).
3. Studyofdevelopmentalstages(abovementioned)byraisingchickembryointhe laboratory.
4. Studyof thedevelopmental stages and lifecycleofDrosophila from stock culture.
5. Studyof differenttypes ofplacenta.
6. Project report onDrosophila culture/chick embryo development.
Page 52 of 54
SUGGESTED READINGS
1. BalinskyBIandFabianBC (1981)AnIntroduction to Embryology.5thEdition.
International ThompsonComputerPress.
2. Gilbert SF(2010)Developmental Biology. 9thEdition. SinauerAssociates,Inc.,USA.
3. Kalthoff(2008) Analysis of Biological Development. 2ndEdition. McGraw-Hill, New York.
4. MohantyPK(2000)Illustrated DictionaryofBiology. Kalyani Publishers,Ludhiana.
5. WolpertL, BeddingtonR,Jessell T, LawrenceP,MeyerowitzEand Smith J(2002) Principles of
Development. 1stEdition, Oxford UniversityPress,NewYork.
6. Sastry K.V & Shukla Vinita, Developmental Biology, RastogiPublication,Meerut.
THEORY
Unit 1:Concept and scope of Biotechnology, Cloning vectors (Types and characteristics), Restriction
enzymes: Nomenclature, detailed study of Type-II.
Unit 2:Transformation techniques: Calcium chloride method and electroporation, Construction of genomic and
cDNA libraries and screening, Bloting techniques, DNA sequencing, Polymerase Chain Reaction, DNA
finger printing and DNA microarray
Unit3:Production of cloned and transgenic animals: Nuclear transplantation, retroviral method, DNA
microinjection, Applications of Transgenic animals: production of pharmaceuticals, knock out mice,
donor organs, production of transgenic plants: Agrobacterium mediated transformation, Application of
Transgenic plants: insect and herbicide resistant plants.
Unit4:Animal cell culture, Expression of cloned genes, molecular diagnosis of genetic diseases (Cystic
fibrosis/Sickle cell anemia), Recombinant DNA in medicine: insulin/growth hormone, Gene therapy.
PRACTICAL
1. Genomic DNA isolation from E. Coli.
2. Plasmid DNA isolation.
3. Restriction digestion
4. Construction of circular and linear restriction map
5. Calculation of transformation efficiency from the data provided
6. To study the following technique through photographs
Southern Bloting
NothernBloting
DNA sequencing
PCR
DNA fingerprinting
7. Project report on animal cell culture
Page 53 of 54
SUGGESTED READINGS
1. Brown, T. A. Molecular Biology, LabfaxII: Gene cloning and DNA Analysis, II Edition
2. Glick, B. R. and Pasternak, JJ, Molecular Biotechnology-Principles and Application of Recombinant
DNA, IV Edition
3. Griffiths, AJF etal. (2009): An Introduction to Genetic Analysis, IX Edition
4. Snustad, DP and Simmons MJ, Principles of Genetics, V Edition
5. Watson JD, Myers RM, Caudy A and Witkowski JK (2007): Recombinant DNA-Genes and Genomes-A
short Course, III Edition
6. Beauchamp TI and Childress JF (2008): Principles of Biomedical Ethics, VI Edition
Page 54 of 54