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    Experiment No. 3

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    pillisathwik2
    1. This experiment uses paper chromatography to separate the colored components of a red and blue ink mixture. Filter paper is marked and spotted with the ink mixture. 2. The paper is suspended vertically in a jar containing an alcohol/water solvent mixture. The solvent rises up the paper, separating the ink components based on how strongly they adhere to the paper. 3. The distance traveled by each color spot and the solvent front is measured to calculate each component's Rf value using the provided formula. This allows identification of the separate components in the original ink mixture.

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    Experiment No. 3

    Uploaded by

    pillisathwik2
    253 views2 pages
    1. This experiment uses paper chromatography to separate the colored components of a red and blue ink mixture. Filter paper is marked and spotted with the ink mixture. 2. The paper is suspended vertically in a jar containing an alcohol/water solvent mixture. The solvent rises up the paper, separating the ink components based on how strongly they adhere to the paper. 3. The distance traveled by each color spot and the solvent front is measured to calculate each component's Rf value using the provided formula. This allows identification of the separate components in the original ink mixture.

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    experiment no. 3

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    1. This experiment uses paper chromatography to separate the colored components of a red and blue ink mixture. Filter paper is marked and spotted with the ink mixture. 2. The paper is suspended vertically in a jar containing an alcohol/water solvent mixture. The solvent rises up the paper, separating the ink components based on how strongly they adhere to the paper. 3. The distance traveled by each color spot and the solvent front is measured to calculate each component's Rf value using the provided formula. This allows identification of the separate components in the original ink mixture.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    253 views2 pages

    Experiment No. 3

    Uploaded by

    pillisathwik2
    1. This experiment uses paper chromatography to separate the colored components of a red and blue ink mixture. Filter paper is marked and spotted with the ink mixture. 2. The paper is suspended vertically in a jar containing an alcohol/water solvent mixture. The solvent rises up the paper, separating the ink components based on how strongly they adhere to the paper. 3. The distance traveled by each color spot and the solvent front is measured to calculate each component's Rf value using the provided formula. This allows identification of the separate components in the original ink mixture.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    EXPERIMENT 5.

    1 Separate the coloured components present in the mixture ofred and blue
    inks by ascending paper chromatography and find their R, values.
    APPARATUS
    Gas jar, glass rod, filter paper strip (Whatman No. 1 filter paper), jar cover, fine capillary tube.
    REQUIREMENT
    Amixture of red and blue inks, alcohol and distilled water.
    PROCEDURE
    1. Take a Whatman No.1 filter paper strip (20x 2 cm)and draw a line with pencil above 4 cm from one end.
    Draw another line lengthwise from the centre of the paper as shown in Fig. 5.1.

    Lid

    Chromatographic Solvent Glass rod to


    paper front hang paper
    Separated
    spots of Cromatographic
    Original line about different paper
    4 cm above the edge
    constituents Jar
    Original spot
    of the Pure
    Point to put the mixture solvent
    spot of the mixture

    Fig. 5.1. Spotting of the mixture.


    Fig. 5.2. Paper chromatography.
    2. With the help of finecapillary tube,put a drop of
    in air. Put another drop on the same spot and the mixture of red and blue inks at the pointP. Let it dry
    mixture. dry again. Repeat 2-3 times, so that the spot is rich in
    3. Suspend the filter paper vertically in a gas jar
    containing the solvent (eluent) with the help a glass rod
    in such a way.that the pencil line (and the
    spot) remains about 2 cm above the solvent levelof(alcohol
    distilled water in 1:1ratio) (Fig. 5.2). and
    4. Cover the jar and keep it
    inks. After the solvent has undisturbed. Notice the rising solvent along with the mixture of red and
    risen about 15 cm you will notice two different blue
    the filter paper. spots of blue and red colours 0!
    5. Take the filter paper out of the jar and mark the distance that the solvent has risen on the paper with a
    pencil. This is called the solvent front.
    6. Dry the paper. Put pencil marks in the centre of the blue and red spots.
    7. Measure the distance of the two spots from the original line and the distance of the solvent from the
    original line.
    8. Calculate the R, values of the blue and red inks by using the formula :
    Distance travelled by the blue or red ink from the point of application
    R,= Distance travelled by the solvent from the original line

    Chromatographic
    paper

    Solvent front

    Spots of different
    constituents

    Original spot of
    the mixture
    P

    Fig. 5.3. Calculation of R, values.

    OBSERVATIONS AND CALCULATIONS

    Substance Distance travelled Distance travelled R, Value


    by different components by solvent

    Red ink + Blue ink A cm (Red ink) X cm A/X

    B cm (Blue ink) X cm B/X

    PRECAUTIONS
    1. Use good quality pencil for drawing the reference line so that the mark does not dissolve in the solyent in
    which the chromatography is carried out.
    2. Always make use of a fine apillary tube.
    3. Keep the jar undisturbed and covered during the experiment.
    4. A spot should be small and rich in mixture.
    5. Allow the spot to dry before putting the strip in the jar.
    6. Keep the strip erect. Do not let it to be curled.
    7. Donot allow the spot todip in the solvent.

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