Horizontal Cylindrical Pressure Autoclave

BKQ-H150/200/300/400/500
User Manual

Model

BIOBASE GROUP
Version 2020.08
 Content
1.Features............................................................................................................................................... 2
2.Specifications...................................................................................................................................... 3
3.Installation........................................................................................................................................... 6
4.Operation............................................................................................................................................. 8
5.Notice................................................................................................................................................ 11
6.Maintenance...................................................................................................................................... 12

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 1.Features
Model BKQ-H150 BKQ-H200 BKQ-H300 BKQ-H400 BKQ-H500
Capacity 150L 200L 280L 400L 500L
Chamber Φ440*1000 Φ515*1000 Φ600*1000 Φ700*1100 Φ440*1300
size(mm)
Working 0.22Mpa
Pressure
Temp range 105-134℃
Sterilization 1-60 min
time
Drying time 0-60 min
Power 9KW 12KW 18KW
consumption
Power supply 220V/380V,50/60Hz 380V,50Hz
External 1400*600* 1400*670*1 1400*770*1 1430*880*1 1900*900*1
size(W*D*H) 1300 650 780 830 820
mm
Gross 320 350 465 530 580
weight(kg)

 interlock device for safety:

 the steam can’t go through to chamber when the door haven’t close, and the pilot off to
inform. You can processing dry operat.
 when the pressure inner over 0.027Mpa, the door be lock and not be open.
 if you mistake open the door in the course of start, the jacket valve auto off and open
the chamber valve.
 if you not choose the sterile time and start the machine, buzzer sound.
 lack water the pilot on, heating and sterile pilot off, the buzzer sound and auto release
steam.
 overload and leakage protection, when leakage or over current occur, it auto cut off the
power.
 temperature controller not work and the inner pressure over 0.165 Mpa, the safety
valve auto start open.

2
 2.Specifications
 The main body is composed of a chamber and steam-jacket with closed double jacket. The
chamber wall will be pre-heated as steam enters and fills the jacket up. A main valve is
connected between the chamber and the jacket, which is turned to “sterilize” position and
admit steam from jacket to chamber to make articles moist and heated. The
above-mentioned method ensures requirements for pre-heating, sterilizing, drying and
following operations.
 The vaporizer, gauge in front above easy watch, the thermometer on the side, all the pilots
and display in front lower part control box for easy operating. (fig.1)

POWER

POWER ENT DOOR LAMP WATER HEATING STERILIZING OVER HEATING

30
20 40

10 50

0 60
min

ENT STOP
TIMER TEMP.CONTROL

Fig1
each letter mark for the role:
 pilot display
power------- power start, pilot on.
close door--- pilot on.
start-------pilot on
water in----auto fill the water,the pilot on to high level the pilot off.
heating-----pilot on to sterile temperature the pilot off.
sterilizing-----pilot on to sterilize end the pilot off.
overheat----after heating water lack the pilot on, press emergency stop button or turn off
dealing the error, restart the machine the pilot off.
 power switch

3
 turn on the breaker, the machine enter the state of standby.
 the knob for setting auto program select.
sterilizing temperature ( range 105℃~134℃)
sterilizing time (range 1~60min.)
 key start
press key start, the auto program sterilizing or dry begin, repeat press useless.
 key emergency stop
press the key emergency stop recovered to connect power, vaporizer, and electric valve
open all and release, pilot start, heat, sterilize off, repeat press useless.

 The jacket is coated with a cover, in which insulated liner of glass fabric is filled, for the sake
of saving energy, as well as preventing ambient temperature from rising excessively

 The door opening mechanism adopts a dial of radial-bar with self-lock and two grade
inter-lock safety devices, first move safety closing handle when you close the door, the
angle 45°with the radial-bar connection center, the radial-bar enter the close position
complete, then turn the handle again, the dial go in axle direction, the center dial be locked
by pin.when the inner pressure over 0.027Mpa, the interlock auto enter to prevent mistake
operation, until the inner pressure lower than 0.027Mpa, interlock device turn back but
the dial keep lock by pin, the handle can be rotate counter clockwise,the center dial back
out in axle way, the seal became useless, the spare steam be release to zero, that is release
the pressure first then open the door,until the handle returned to outer can you move closing
handle and returned radial-bar to open the door.

 With program function, it’ll auto release the air and condense to balance the temperature in
chamber.

 With the device to stabilizing the steam pressure in the course of heating, the range
0.05~0.15Mpa, related temperature 105~134℃,

 With timer device, the range 1~60min. the knob on the panel of the control box.

 With high water level device, you push the key start, the pilot water in on, the water auto
enters to the high level of the tank (about 26L)and heating right way, meanwhile the pilot
off.

 auto control problem

 the steam can’t go through to chamber when the door haven’t close, and the pilot off to
inform. You can processing dry operate.

 if you mistake open the door in the course of start, the jacket valve auto off and open the
chamber valve. with mechanic interlock pressure ≥0.027MPa
 if you not choose the sterile time and start the machine, buzzer sound.

4
 with lower level protection device, when lack water the pilot on,auto cut off the power,
heating and sterile pilot off, the buzzer sound and auto release
steam.
 overload and leakage protection, when leakage or over current occur, lug
it auto cut off the power.the pilot off on panel.
 An ordinary lift safety valve connected to the jacket is installed at
the top of the sterilizer. It cannot open to release overpressure steam
until the steam in jacket overpasses set pressure of safety valve.
When steam pressure falls to the normal value, it automatically
closes. The set pressure for safety valve is 0.165 MPa~0.17 MPa (See Fig2)
 manual emergency switch valve
Fig2
 when the unit suddenly break the power and you need take the objects be sterilize urgent,
you can manually turn on the bulb valve on the rear release the pressure, until the gauge back
to zero you can take the objects.
 manual maintenance switch valve
 When you need clean the vaporizer, you can open the manual valve at the bottom and release
the dirty water.
 magnetic valve function:
 water-in valve: press key start, it auto fill the water to high level then turn off.
 condensation valve: power connect and press key start auto release,heating and temperature
≤108℃ auto turn on, ≥108℃ auto turn off, press emergency key
 release valve: power connect and press key start auto release, after the auto water in to high
level then turn off. Sterilize end, press emergency key auto release on.
 sterilize valve: power connect and press key start auto air-in, after the auto water in to high
level,the door haven’t closed turn off, otherwise turn on.sterilize end press emergency key
auto release on.

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 3.Installation
 for installation. More floor space around the sterilizer is set aside for operating and repairing.
The Min. Installation size for single door 200×93×182cm, for double doors
200×93×182cm.(see fig.3)
 Connect inlet with pure water supply and outlet with drain pipelines so as to make daily
operation simpler.
 Extend exhaust pipe outside to avoid vapor throughout inside, so as to affect operation
inside.
 During sterilizing, condense will be discharged through the condense ejector. It is suggested
that a cone-shaped funnel is supplied to join the exhaust pipe and drain pipe. Thus the
drainage of condense may be easily observed, as well as prevent drain flowing back, in case
of drain line clogged.
 The unit has been fully inspected before leaving the factory, but after installation by users,
training should be performed according to manual instructions. In case that anything
abnormal occurs, it should be resolved until the unit is in operation.

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 safety valve
magnet valve sterilizer

jacket & chamber gauge

magnet valve quick release

door

vaporize

7
electric control box

Fig3
电源
POWER

POWER ENT DOOR LAMP W ATER HEATING STERILIZING OVER HEATING

电源 启动 门 进水 加热 灭菌 断水
magnet valve condense slow release
JIBIMED

interlock device
30
20 40

10 50

0 60
min

启动 紧停全排
时间
TIMER
ENT STOP
温度控制器
TEMP.CONTROL
temperature sensor

high,low level
magnet valve water-in
electric type single door installation
 4.Operation
 Operation procedures:
 manual operation
 connection water----- trun on the water source
 connection the power-----pilot display
 Pile --------The articles (to sterilize) should be well packed and separated certain distance
from each other in chamber, so as to leave free passage for steam. Dressing package cannot
be close to chamber door, otherwise condense drops along door into dressing and prolongs
drying time. In general dressing package should be no more than 20×20×10cm, otherwise
the period of sterilizing time would be prolonged .If sterilize solution, pour it into a rigid
heat-resistant flask, do not exceed 3/4 containment of the flask. Stuff mouth with cotton and
gauze, and bind it with yarn. Be sure not to use a non-perforated rubber stopper or cork, or
the flask will be broken. Put the flask onto a larger enamelware or metal tray, in case the
explosion of the bottle occurs, the liquid won't flow away to pollute and damage the inside
of the sterilizer.
 Close the door, turn the safety handle clockwise to the center position, to make radial arms
into the door ring, then turn the octagonal hand wheel clockwise along axis, till the door is
steam-tight (Avoid over-tightening or it will shorten the life of the door gasket). Then the
door close indicator lights. Solenoid valve is closed so that steam pipelines admit the steam
into chamber. For the double-doors unit, till both the front and the back doors are all closed,
then the door close indicator lights.
Preset Sterilizing Time:
The customer can preset the required sterilizing time (see form 1) according to following table.
Turn the timer knob on the time line you wanted clockwise. When it reached the preset
temperature, the pilot of time light, the timer start to count automatically.
Reference table to sterilization time and temperature ( form I)
Objects Required heat Steam Pressure Relative steam
preservation time Mpa Temperature ℃
(min)
Rubber goods 15 0.1 – 0.11 121
Surgical dressings 15 - 20 0.1 – 0.22 121– 134
Utensils 8 0.1 – 0.22 121– 134
Instruments 10 0.1 – 0.22 121– 134
Solution in flasks 20 - 40 0.1 – 0.145 121– 126

1) Sterling temperature（115℃~134℃）
2) Sterling time (1~60min)

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 Rotate of counter clockwise,
the pressure and temperature high
in the sterilizing room

Fig4
 setting fulfill---- press key SET. Pilot door,start, water-in on. Auto water-in to high level and
the pilot off, and heating pilot on, this time you press start the buzzer sound to inform you
setting temperature and time.
 Press key start----- sterilizing begin.
 End---- buzzer sound, when the inner gauge show zero, open the door the buzzer stop.
 At the end of the sterilization period, in case of rapid decrease in pressure of chamber,
solution or culture media in flasks would boil over or be spoiled. Therefore, turn the main
valve knob to “slow exhaust” position to release the steam in the chamber slowly. Also cut
off steam supply, let pressure and temperature in chamber drop naturally. Another way is
provided to turn pressure adjustment switch to “close ”position till chamber pressure gauge
is reduced to “0”. Wait a few minutes after main valve is turned to “slow exhaust”, door can
be opened.
 End------If no more sterilization is needed, switch off the key emergency, to exhaust the
steam in jacket and chamber, and open the door slightly to release steam from the chamber
in order to keep its inner surface dry. And then cut off the power.

 auto program control

 auto water-in,( from tap to main tank)-----heating ( auto release the
condense)-----sterilizing(timing)------exhaust steam-----end

 auto display pilot indicator in sterilizing processing

 power pilot on----door pilot on----start pilot on----water in pilot on(high level off)----heating pilot
on----sterilizing pilot on (heating pilot flash)----heating,sterilizing pilot off,buzzer sound---- open
the door the pilot off, buzzer stop.

 dry fully auto program

 after sterilize time, according different demand , be drying by the jacket,and meanwhile open the
door around 40~50mm for cooling.
 manual operation ( setting program select)
1) Sterilizing temperature 134℃
2) Sterilizing time 1~60min.

 dry auto program control

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  heating(auto release the condense)----drying(timing)----end
 auto display pilot indicator in drying processing
 power pilot on---- start pilot on---- heating pilot on---- sterilizing pilot on (heating pilot flash)----
heating,sterilizing pilot off

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 5.Notice
 Do not sterilize different articles at the same time, such as dressing and solution or the same
article but has different sterilization requirements
 Check the water level indicator before using the unit, see whether it has sufficient water in tank,
if not, add more water when the water level below water line, adding water must in no pressure
state.
 if alarm pilot for the water level on, it’ll auto exhaust the steam, press emergency key or
turn off the power then restart the power,the adding water going on, this time the pilot off,
then press key start to going on the heating.
 If you want adding water in the standby,press emergency key, the machine recover to the
state of connect power, vaporize & magnet valve open to release, pilot start,heating and
sterilize off, press repeat useless. Thus it auto adding the water and going on heating.
 The handle of safety valve draw up 1-2times per week,make it smoothly.
 Electric connector should tighten often to prevent loose and overheat.
 Keep the sterilizer clean and dry, particularly if not used for a certain period of time, please clean
up for the purpose of no rust. And lubricate the active parts regularly.
 The unit should be operated in normal conditions, as well as periodically checked and maintained
by professional personnel.
 Operating regulations should be set up beside the unit, for the convenience of reference and
conformance.
 Protective earth should be provide to ensure safety.
 In the course of sterilizing, if the safety gauge release,means the jacket pressure over the working
pressure, you should inspect the pressure controller gauge and safety valve.

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 6.Maintenance
 Gasket:
The door gasket of rubber should be replaced timely, if steam leakage occurs.
 Pressure gauge:
If pressure gauge pointer doesn’t return to “0” or reading isn’t accurate, repairs should be made and
then see if it up to standard pressure gauge. If not, replace it with new one.
 Thermometer:
There are one thermometer, equipped in condenser outlet at the bottom of chamber, for test the
temperature between condensate and chamber ,If temperature difference of them is large and the
mercurial thermometer is in good order, the dial one should be repaired and checked.
 Pressure controller:
It is equipped with temperature pressure controller preset: 0.05MPa,~0.15Mpa, after be using for a
period and large difference please replace.

（form 2）
Breakdown Possible cause Removes method
Dirt accumulated
Unscrew the cover, clean the
between the seats of the
Required temperature ejecto
valve
in the chamber can’t be
reached The front part to the
Dredge it
elector blocked
The temperature in
Distance between top Fine adjust the temperature
chamber rises too
and seat of the valve is controller on the bottom of
slowly, A great quantity
wide control box
of steam leaks out
 Electric-thermal tube
There are three U-shape electric-thermal tubes at the bottom of water tank. each 3KW for AC380V
three phase /AC110V(single phases).The unit input of power supply should conform to nameplate.

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BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: export@biobase.com
Complaints: customer_support@biobase.cc
After-sales service: service_sd@biobase.cc; service_ivd@biobase.cc
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com