Certificate of Analysis - Certified Reference Material

Polynuclear Aromatic Hydrocarbons Mix

Product no.: CRM47543
Lot no.: LRAD2030
Expiry Date: April 2025
Manufacturing Date: April 2022
Storage: REFRIGERATE
Solvent/Matrix: DICHLOROMETHANE/BENZENE 1:1
Certificate version: LRAD2030.01 (Note: Certificates may be updated due to
the availability of new data. Check our website at:
www.sigma-aldrich.com for the most current version.)

Certified Values:
Certified Raw Material Raw Material
Value Units Purity,% Lot
Analyte
NAPHTHALENE 2000 ± 58 µg/mL 99.8 LRAD0645
CAS# 91-20-3
2-METHYLNAPHTHALENE 2002 ± 61 µg/mL 97.2 11608EA
CAS# 91-57-6
1-METHYLNAPHTHALENE 2000 ± 61 µg/mL 98.6 LC13481
CAS# 90-12-0
ACENAPHTHYLENE 2000 ± 61 µg/mL 96.5 MKBC3740
CAS# 208-96-8
ACENAPHTHENE 2000 ± 63 µg/mL 99.9 MKCK2310
CAS# 83-32-9
FLUORENE 2000 ± 62 µg/mL 99.2 LRAC8730
CAS# 86-73-7
PHENANTHRENE 2002 ± 67 µg/mL 98.1 LRAC8436
CAS# 85-01-8
ANTHRACENE 2000 ± 61 µg/mL 99.2 LRAC8733
CAS# 120-12-7
FLUORANTHENE 2000 ± 62 µg/mL 98.8 MKCL4099
CAS# 206-44-0
PYRENE, 5000MG, NEAT 2002 ± 63 µg/mL 98.6 LRAC8732
CAS# 129-00-0
BENZO (A) ANTHRACENE 2000 ± 59 µg/mL 99.0 LC21542
CAS# 56-55-3
CHRYSENE 2000 ± 67 µg/mL 99.0 21L74
CAS# 218-01-9
BENZO (B) FLUORANTHENE 2004 ± 62 µg/mL 99.6 LRAC8201
CAS# 205-99-2
BENZO (K) FLUORANTHENE 2000 ± 76 µg/mL 99.0 012019K
CAS# 207-08-9
BENZO(A)PYRENE 2000 ± 63 µg/mL 99.0 SLCD4874
CAS# 50-32-8
INDENO (1,2,3-CD) PYRENE 2000 ± 92 µg/mL 99.5 ER082107-02
CAS# 193-39-5
DIBENZ (A,H) ANTHRACENE 1997 ± 80 µg/mL 99.2 ER032211-01
CAS# 53-70-3

Sigma-Aldrich RTC, 2931 Soldier Springs Rd. Laramie, WY 82070, USA;

Tel. 1 307-742-5452; Fax 1 855-831-9211; www.sigmaaldrich.com
Sigma-Aldrich RTC is a subsidiary of Merck KGaA, Darmstadt, Germany.

Certificate Page 1 of 4 Certificate version 01

 Certified Raw Material Raw Material
Value Units Purity,% Lot
Analyte
BENZO (G,H,I) PERYLENE 2001 ± 72 µg/mL 96.0 0000138084
CAS# 191-24-2

ASSAY Method
METHOD: GC (IN-HOUSE )
Column: SPB-5, 30 m × 0.53 mm I.D., 1.5 µm film thickness
Carrier Gas: H2 Flow Rate: 4.5 mL/min
Inlet Temperature: 250 °C Injection Volume: 1 µL
Injection Mode: 20:1
Temperature Program: 120 °C (Hold 1 min) @ 6 °C/min to 300 °C (Hold 17 min)
Detector: FID Temperature: 320 °C

Elution details:

EO TIME ANALYTE
1 5.08 Naphthalene
2 6.78 2-Methylnaphthalene
3 7.08 1-Methylnaphthalene
4 9.51 Acenaphthylene
5 10.10 Acenaphthene
6 11.90 Fluorene
7 15.30 Phenanthrene
8 15.50 Anthracene
9 19.80 Fluoranthene
10 20.60 Pyrene
11 25.20 Benzo(a)anthracene
12 25.30 Chrysene
13 29.10 Benzo(b)fluoranthene
14 29.20 Benzo(k)fluoranthene
15 30.10 Benzo(a)pyrene
16 34.10 Indeno(1,2,3-cd)pyrene
17 34.20 Dibenz(a,h)anthracene
18 35.10 Benzo(g,h,i)perylene
Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken
chain of comparisons. The balance used to weigh raw materials is accurate to
+/-0.0001 g and calibrated regularly using mass standards traceable to NIST. All
dilutions were performed gravimetrically. Additionally, individual analytes are
traceable to NIST SRMs where available and specified above.

Certificate Page 2 of 4 Certificate version 01 LRAD2030

 Measurement method: Where applicable, the assigned value is based on a purity determination by mass
balance and gravimetrically prepared value.
Intended use: Intended for R&D and Analytical Use only. Not for drug, household or other uses.

Minimum sample size: 1 µL

Packaging: 1 mL in amber ampule
Instructions for handling Use on the as is basis. The internal pressure of the container may be slightly
and correct use: different from the atmospheric pressure at the user`s location. Open slowly and
carefully to avoid dispersion of the material.
Health and safety All chemical reference materials should be considered potentially hazardous and
information: should be used only by qualified laboratory personnel. Please refer to the Safety
Data Sheet for detailed information about the nature of any hazard and
appropriate precautions to be taken.
Accreditation: Sigma-Aldrich RTC is accredited by the US accreditation authority ANAB as a
registered reference material producer AR-1470 in accordance with ISO 17034.
Certificate issue date: 15 APR 2022

Andy Ommen - QC Manager Scott Stetler - QA Manager

Details on metrological This standard has been gravimetrically prepared using balances that have been
traceability: fully qualified and calibrated to ISO 17025 requirements. All calibrations utilize
NIST traceable weights which are calibrated externally by a qualified ISO 17025
accredited calibration laboratory to NIST standards. Qualification of each balance
includes the assignment of a minimum weighing by a qualified and ISO 17025
accredited calibration vendor taking into consideration the balance and installed
environmental conditions to ensure compliance with USP tolerances of NMT
0.10% relative error. Fill volume to predetermined specifications is
gravimetrically verified throughout the dispensing process using qualified and
calibrated balances. Further traceability to a corresponding Primary Standard may
be achieved through a direct comparison assay. Where a Primary Standard is
available, the assay value will be included in the specified section of the COA.

Associated uncertainty: Ucrm - Uncertainty values in this document are expressed as Expanded
Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is
derived from the combined standard uncertainty multiplied by the coverage factor
k, which is obtained from a t-distribution and degrees of freedom. The
components of combined standard uncertainty include the uncertainties due to
characterization, homogeneity, long term stability, and short term stability
(transport). The components due to stability are generally considered to be
negligible unless otherwise indicated by stability studies. The mathematical
representation of the Ucrm calculation is as follows:
u CRM  2
u char  u hom
2
ogeneity  u stability
2

Homogeneity assessment: Homogeneity was assessed in accordance with ISO Guide 35. Completed units
were sampled using a random stratified sampling protocol. The results of
chemical analysis were then compared by Single Factor Analysis of Variance
(ANOVA). The uncertainty due to homogeneity was derived from the ANOVA.
Heterogeneity was not detected under the conditions of the ANOVA.
Stability assessment: Significance of the stability assessment will be demonstrated if the analytical
result of the study and the range of values represented by the Expanded
Uncertainty do not overlap the result of the original assay and the range of its
values represented by the Expanded Uncertainty. The method employed will
usually be the same method used to characterize the assay value in the initial
evaluation.

Certificate Page 3 of 4 Certificate version 01 LRAD2030

 Certificate of analysis revision history:

Certificate version Date Reason for version

LRAD2030.01 15 APR 2022 Original release date
Disclaimer: The purchaser is required to determine the suitability of this product for
any particular application. Sigma-Aldrich RTC makes no warranty of any kind, express
or implied, other than its products meet all quality control standards set by
Sigma-Aldrich RTC. We do not guarantee that the product can be used for any
particular application.
The vibrant M, Supelco, TraceCERT and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.
All other trademarks are the property of their respective owners. Detailed information on trademarks is available via
publicly accessible resources. © 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Certificate Page 4 of 4 Certificate version 01 LRAD2030