SAFETY DATA SHEET Version 6.

7
Revision Date 02.02.2023
according to Regulation (EC) No. 1907/2006 Print Date 21.02.2023
GENERIC EU MSDS - NO COUNTRY SPECIFIC DATA - NO OEL DATA

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifiers
Product name : Chloroform
Product Number : 288306
Brand : Sigma-Aldrich
Index-No. : 602-006-00-4
REACH No. : 01-2119486657-20-XXXX
CAS-No. : 67-66-3

1.2 Relevant identified uses of the substance or mixture and uses advised against
Identified uses : Laboratory chemicals, Manufacture of substances
1.3 Details of the supplier of the safety data sheet
Company : Sigma-Aldrich Chemie GmbH
Eschenstrasse 5
D-82024 TAUFKIRCHEN
Telephone : +49 (0)89 6513-1130
Fax : +49 (0)89 6513-1161
E-mail address : technischerservice@merckgroup.com
1.4 Emergency telephone
Emergency Phone # : 0800 181 7059 (CHEMTREC Deutschland)
+49 (0)696 43508409 (CHEMTREC
weltweit)

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008
Acute toxicity, Oral (Category 4), H302
Acute toxicity, Inhalation (Category 3), H331
Skin irritation (Category 2), H315
Eye irritation (Category 2), H319
Carcinogenicity (Category 2), H351
Reproductive toxicity (Category 2), H361d
Specific target organ toxicity - single exposure (Category 3), Central nervous system, H336
Specific target organ toxicity - repeated exposure, Oral (Category 1), Liver, Kidney, H372
For the full text of the H-Statements mentioned in this Section, see Section 16.

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2.2 Label elements
Labelling according Regulation (EC) No 1272/2008
Pictogram

Signal Word Danger

Hazard statement(s)
H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H331 Toxic if inhaled.
H336 May cause drowsiness or dizziness.
H351 Suspected of causing cancer.
H361d Suspected of damaging the unborn child.
H372 Causes damage to organs (Liver, Kidney) through prolonged or
repeated exposure if swallowed.
Precautionary statement(s)
P202 Do not handle until all safety precautions have been read and
understood.
P301 + P312 IF SWALLOWED: Call a POISON CENTER/ doctor if you feel
unwell.
P302 + P352 IF ON SKIN: Wash with plenty of water.
P304 + P340 + P311 IF INHALED: Remove person to fresh air and keep comfortable
for breathing. Call a POISON CENTER/ doctor.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue
rinsing.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
Supplemental Hazard none
Statements
For use in industrial installations only.

Reduced Labeling (<= 125 ml)

Pictogram

Signal Word Danger

Hazard statement(s)
H331 Toxic if inhaled.
H351 Suspected of causing cancer.
H372 Causes damage to organs through prolonged or repeated
exposure if swallowed.
H361d Suspected of damaging the unborn child.
Precautionary statement(s)
P202 Do not handle until all safety precautions have been read and
understood.
P304 + P340 + P311 IF INHALED: Remove person to fresh air and keep comfortable
for breathing. Call a POISON CENTER/ doctor.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
Supplemental Hazard none

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 Statements
2.3 Other hazards
This substance/mixture contains no components considered to be either persistent,
bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at
levels of 0.1% or higher.

SECTION 3: Composition/information on ingredients

3.1 Substances
Synonyms : Trichloromethane
Methylidyne trichloride

Formula : CHCl3
Molecular weight : 119,38 g/mol
CAS-No. : 67-66-3
EC-No. : 200-663-8
Index-No. : 602-006-00-4
Component Classification Concentration
Chloroform
CAS-No. 67-66-3 Acute Tox. 4; Acute Tox. <= 100 %
EC-No. 200-663-8 3; Skin Irrit. 2; Eye Irrit.
Index-No. 602-006-00-4 2; Carc. 2; Repr. 2; STOT
RE 1; H302, H331, H315,
H319, H351, H361d, H372
Concentration limits:
20 %: STOT SE 3, H336;

ethanol
CAS-No. 64-17-5 Flam. Liq. 2; Eye Irrit. 2; >= 1 - < 10
EC-No. 200-578-6 H225, H319 %
Index-No. 603-002-00-5 Concentration limits:
>= 50 %: Eye Irrit. 2A,
H319;

For the full text of the H-Statements mentioned in this Section, see Section 16.

SECTION 4: First aid measures

4.1 Description of first-aid measures
General advice
First aiders need to protect themselves. Show this material safety data sheet to the doctor
in attendance.
If inhaled
After inhalation: fresh air. Immediately call in physician. If breathing stops: immediately
apply artificial respiration, if necessary also oxygen.
In case of skin contact
In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with
water/ shower. Consult a physician.

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 In case of eye contact
After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact
lenses.
If swallowed
After swallowing: immediately make victim drink water (two glasses at most). Consult a
physician.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section
2.2) and/or in section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available

SECTION 5: Firefighting measures

5.1 Extinguishing media
Suitable extinguishing media
Use extinguishing measures that are appropriate to local circumstances and the
surrounding environment.
Unsuitable extinguishing media
For this substance/mixture no limitations of extinguishing agents are given.
5.2 Special hazards arising from the substance or mixture
Carbon oxides
Hydrogen chloride gas
Not combustible.
Ambient fire may liberate hazardous vapours.
5.3 Advice for firefighters
Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by
keeping a safe distance or by wearing suitable protective clothing.
5.4 Further information
Suppress (knock down) gases/vapors/mists with a water spray jet. Prevent fire
extinguishing water from contaminating surface water or the ground water system.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures
Advice for non-emergency personnel: Do not breathe vapors, aerosols. Avoid substance
contact. Ensure adequate ventilation. Evacuate the danger area, observe emergency
procedures, consult an expert.
For personal protection see section 8.
6.2 Environmental precautions
Do not let product enter drains.
6.3 Methods and materials for containment and cleaning up
Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions
(see sections 7 and 10). Take up carefully with liquid-absorbent material (e.g.
Chemizorb®). Dispose of properly. Clean up affected area.
6.4 Reference to other sections
For disposal see section 13.

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SECTION 7: Handling and storage
7.1 Precautions for safe handling
Advice on safe handling
Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols.
Hygiene measures
Immediately change contaminated clothing. Apply preventive skin protection. Wash hands
and face after working with substance.
For precautions see section 2.2.
7.2 Conditions for safe storage, including any incompatibilities
Storage conditions
Tightly closed. Keep in a well-ventilated place. Keep locked up or in an area accessible only
to qualified or authorized persons.
Storage class
Storage class (TRGS 510): 6.1D: Non-combustible, acute toxic Cat.3 / toxic hazardous
materials or hazardous materials causing chronic effects
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated

SECTION 8: Exposure controls/personal protection

8.1 Control parameters
Ingredients with workplace control parameters
8.2 Exposure controls
Personal protective equipment

Eye/face protection
Use equipment for eye protection tested and approved under appropriate
government standards such as NIOSH (US) or EN 166(EU). Safety glasses
Skin protection
This recommendation applies only to the product stated in the safety data sheet,
supplied by us and for the designated use. When dissolving in or mixing with other
substances and under conditions deviating from those stated in EN374 please
contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell,
Internet: www.kcl.de).
Full contact
Material: Viton®
Minimum layer thickness: 0,7 mm
Break through time: 480 min
Material tested:Vitoject® (KCL 890 / Aldrich Z677698, Size M)
This recommendation applies only to the product stated in the safety data sheet,
supplied by us and for the designated use. When dissolving in or mixing with other
substances and under conditions deviating from those stated in EN374 please
contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell,
Internet: www.kcl.de).
Splash contact
Material: butyl-rubber

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 Minimum layer thickness: 0,7 mm
Break through time: 10 min
Material tested:Butoject® (KCL 898)
Body Protection
protective clothing
Respiratory protection
required when vapours/aerosols are generated.
Our recommendations on filtering respiratory protection are based on the following
standards: DIN EN 143, DIN 14387 and other accompanying standards relating to
the used respiratory protection system.
Recommended Filter type: Filter type AX
The entrepeneur has to ensure that maintenance, cleaning and testing of respiratory
protective devices are carried out according to the instructions of the producer.
These measures have to be properly documented.
Control of environmental exposure
Do not let product enter drains.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
a) Physical state liquid, clear
b) Color colorless
c) Odor sweet
d) Melting Melting point/range: -63 °C - lit.
point/freezing point
e) Initial boiling point 60,5 - 61,5 °C - lit.
and boiling range
f) Flammability (solid, No data available
gas)
g) Upper/lower No data available
flammability or
explosive limits
h) Flash point - Regulation (EC) No. 440/2008, Annex, A.9does not flash
i) Autoignition No data available
temperature
j) Decomposition Distillable in an undecomposed state at normal pressure.
temperature
k) pH No data available
l) Viscosity Viscosity, kinematic: No data available
Viscosity, dynamic: No data available
m) Water solubility 8,7 g/l at 23 °C - OECD Test Guideline 105- soluble
n) Partition coefficient: No data available
n-octanol/water
o) Vapor pressure 210 hPa at 20 °C

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 p) Density 1,49 g/cm3 at 25 °C
Relative density No data available
q) Relative vapor No data available
density
r) Particle No data available
characteristics

s) Explosive properties No data available

t) Oxidizing properties none
9.2 Other safety information
Solubility in other organic solvent at 20 °C
solvents - miscible
Relative vapor 4,12 - (Air = 1.0)
density

SECTION 10: Stability and reactivity

10.1 Reactivity
No data available
10.2 Chemical stability
Sensitivity to light heat-sensitive
The product is chemically stable under standard ambient conditions (room temperature) .
Contains the following stabilizer(s):
ethanol (>=0,5 - <=1 %)
10.3 Possibility of hazardous reactions
Risk of explosion with:
Ammonia
Amines
nitrogen oxides
bases
Oxygen
alkali amides
organic nitro compounds
strong alkalis
Fluorine
peroxi compounds
Alkaline earth metals
Alkali metals
Powdered metals
Methanol
with
alcoholates
Methanol
with
strong alkalis
Iron
in powder form
various alloys

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 sensitive to shock
Methanol
with
Sodium hydroxide
magnesium
in powder form
Oxygen
with
alkali compounds
Aluminum
in powder form
Acetone
with
alkali compounds
Potassium
sensitive to shock
sodium
sensitive to shock
Violent reactions possible with:
phosphines
bis(dimethylamino)dimethyl tin
nonmetallic hydrogen compounds
Powdered metals
Light metals
Ketones
mineral acids
Strong oxidizing agents
semimetallic hydrogen compounds

10.4 Conditions to avoid

no information available
10.5 Incompatible materials
rubber, various plastics
10.6 Hazardous decomposition products
In the event of fire: see section 5

SECTION 11: Toxicological information

11.1 Information on toxicological effects
Acute toxicity
Oral: No data available
LD50 Oral - Rat - male - 908 mg/kg
(OECD Test Guideline 401)
Acute toxicity estimate Oral - 908 mg/kg
(ATE value derived from LD50/LC50 value)
Acute toxicity estimate Inhalation - 4 h - 3,12 mg/l - vapor(Calculation method)

LC50 Inhalation - Rat - 6 h - 9,17 mg/l - vapor

Acute toxicity estimate Inhalation - Expert judgment - 4 h - 3,1 mg/l - vapor

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 Dermal: No data available
Skin corrosion/irritation
Remarks: No data available
Skin - Rabbit
Result: Irritating to skin. - 24 h
Remarks: (ECHA)
Remarks: Drying-out effect resulting in rough and chapped skin.
Skin - Rabbit
Result: slight irritation
Remarks: (IUCLID)
Serious eye damage/eye irritation
Remarks: No data available
Eyes - Rabbit
Result: Irritating to eyes.
Remarks: (ECHA)
Remarks: (Regulation (EC) No 1272/2008, Annex VI)
Respiratory or skin sensitization
Maximization Test - Guinea pig
Result: negative
(Regulation (EC) No. 440/2008, Annex, B.6)
Germ cell mutagenicity
No data available
Test Type: Ames test
Test system: Escherichia coli/Salmonella typhimurium
Metabolic activation: with and without metabolic activation
Result: negative
Remarks: (ECHA)
Test Type: unscheduled DNA synthesis assay
Test system: Liver
Metabolic activation: without metabolic activation
Result: negative
Remarks: (ECHA)

Test Type: Micronucleus test

Species: Rat
Cell type: Red blood cells (erythrocytes)
Application Route: Oral
Method: OECD Test Guideline 474
Result: negative

Test Type: unscheduled DNA synthesis assay

Species: Rat
Cell type: Liver cells
Application Route: Oral
Method: OECD Test Guideline 486
Result: negative

Test Type: in vivo assay

Species: Mouse

Application Route: Inhalation

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 Result: negative
Remarks: (ECHA)
Carcinogenicity
Suspected of causing cancer.
Reproductive toxicity
No data available
Suspected of damaging the unborn child.
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available
11.2 Additional Information
Endocrine disrupting properties
Product:
Assessment : The substance/mixture does not contain
components considered to have endocrine
disrupting properties according to REACH Article
57(f) or Commission Delegated regulation (EU)
2017/2100 or Commission Regulation (EU)
2018/605 at levels of 0.1% or higher.
Repeated dose toxicity - Rat - female - Oral - NOAEL (No observed adverse effect level) -
34 mg/kg

To the best of our knowledge, the chemical, physical, and toxicological properties have not
been thoroughly investigated.

Vomiting, Cough, irritant effects, Shortness of breath, respiratory arrest, narcosis,

Dizziness, Nausea, agitation, spasms, inebriation, Headache, Stomach/intestinal disorders,
ataxia (impaired locomotor coordination), cardiovascular disorders
Drying-out effect resulting in rough and chapped skin.
To the best of our knowledge, the chemical, physical, and toxicological properties have not
been thoroughly investigated.

SECTION 12: Ecological information

12.1 Toxicity
No data available
Toxicity to algae static test ErC50 - Chlamydomonas reinhardtii (green algae) - 13,3
mg/l - 72 h
Remarks: (ECHA)
(Chloroform)
Toxicity to bacteria Remarks: (ECHA)
(Chloroform)
Toxicity to flow-through test NOEC - Oryzias latipes - 0,15 mg/l - 9 Months
fish(Chronic toxicity) Remarks: (ECHA)
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 Toxicity to daphnia semi-static test NOEC - Daphnia magna (Water flea) - 6,3 mg/l - 21
and other aquatic d
invertebrates(Chronic Remarks: (ECHA)
toxicity)
12.2 Persistence and degradability
No data available
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
This substance/mixture contains no components considered to be either persistent,
bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at
levels of 0.1% or higher.
12.6 Endocrine disrupting properties
Product:
Assessment : The substance/mixture does not contain components
considered to have endocrine disrupting properties
according to REACH Article 57(f) or Commission
Delegated regulation (EU) 2017/2100 or Commission
Regulation (EU) 2018/605 at levels of 0.1% or higher.

12.7 Other adverse effects

No data available

SECTION 13: Disposal considerations

13.1 Waste treatment methods
Product
See www.retrologistik.com for processes regarding the return of chemicals and
containers, or contact us there if you have further questions.

SECTION 14: Transport information

14.1 UN number
ADR/RID: 1888 IMDG: 1888 IATA: 1888
14.2 UN proper shipping name
ADR/RID: CHLOROFORM, SOLUTION
IMDG: CHLOROFORM, SOLUTION
IATA: Chloroform, SOLUTION
14.3 Transport hazard class(es)
ADR/RID: 6.1 IMDG: 6.1 IATA: 6.1
14.4 Packaging group
ADR/RID: III IMDG: III IATA: III

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14.5 Environmental hazards
ADR/RID: no IMDG Marine pollutant: no IATA: no
14.6 Special precautions for user
Tunnel restriction code : (E)
Further information : No data available

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the
substance or mixture
This material safety data sheet complies with the requirements of Regulation (EC) No.
1907/2006.
Authorisations and/or restrictions on use
REACH - Restrictions on the manufacture, : Chloroform
placing on the market and use of certain
dangerous substances, mixtures and articles
(Annex XVII)

National legislation
Seveso III: Directive 2012/18/EU of the European : ACUTE TOXIC
Parliament and of the Council on the control of
major-accident hazards involving dangerous
substances.

Other regulations
Observe work restrictions regarding maternity protection in accordance to Dir 92/85/EEC or
stricter national regulations where applicable.
Take note of Dir 94/33/EC on the protection of young people at work.
15.2 Chemical Safety Assessment
For this product a chemical safety assessment was not carried out

SECTION 16: Other information

Full text of H-Statements referred to under sections 2 and 3.
H225 Highly flammable liquid and vapor.
H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H331 Toxic if inhaled.
H336 May cause drowsiness or dizziness.
H351 Suspected of causing cancer.
H361d Suspected of damaging the unborn child.
H372 Causes damage to organs through prolonged or repeated exposure if
swallowed.

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 Full text of other abbreviations
ADN - European Agreement concerning the International Carriage of Dangerous Goods
by Inland Waterways; ADR - Agreement concerning the International Carriage of
Dangerous Goods by Road; AIIC - Australian Inventory of Industrial Chemicals; ASTM -
American Society for the Testing of Materials; bw - Body weight; CMR - Carcinogen,
Mutagen or Reproductive Toxicant; DIN - Standard of the German Institute for
Standardisation; DSL - Domestic Substances List (Canada); ECx - Concentration
associated with x% response; ELx - Loading rate associated with x% response; EmS -
Emergency Schedule; ENCS - Existing and New Chemical Substances (Japan); ErCx -
Concentration associated with x% growth rate response; GHS - Globally Harmonized
System; GLP - Good Laboratory Practice; IARC - International Agency for Research on
Cancer; IATA - International Air Transport Association; IBC - International Code for the
Construction and Equipment of Ships carrying Dangerous Chemicals in Bulk; IC50 - Half
maximal inhibitory concentration; ICAO - International Civil Aviation Organization;
IECSC - Inventory of Existing Chemical Substances in China; IMDG - International
Maritime Dangerous Goods; IMO - International Maritime Organization; ISHL - Industrial
Safety and Health Law (Japan); ISO - International Organisation for Standardization;
KECI - Korea Existing Chemicals Inventory; LC50 - Lethal Concentration to 50 % of a
test population; LD50 - Lethal Dose to 50% of a test population (Median Lethal Dose);
MARPOL - International Convention for the Prevention of Pollution from Ships; n.o.s. -
Not Otherwise Specified; NO(A)EC - No Observed (Adverse) Effect Concentration;
NO(A)EL - No Observed (Adverse) Effect Level; NOELR - No Observable Effect Loading
Rate; NZIoC - New Zealand Inventory of Chemicals; OECD - Organization for Economic
Co-operation and Development; OPPTS - Office of Chemical Safety and Pollution
Prevention; PBT - Persistent, Bioaccumulative and Toxic substance; PICCS - Philippines
Inventory of Chemicals and Chemical Substances; (Q)SAR - (Quantitative) Structure
Activity Relationship; REACH - Regulation (EC) No 1907/2006 of the European
Parliament and of the Council concerning the Registration, Evaluation, Authorisation and
Restriction of Chemicals; RID - Regulations concerning the International Carriage of
Dangerous Goods by Rail; SADT - Self-Accelerating Decomposition Temperature; SDS -
Safety Data Sheet; TCSI - Taiwan Chemical Substance Inventory; TECI - Thailand
Existing Chemicals Inventory; TSCA - Toxic Substances Control Act (United States); UN
- United Nations; UNRTDG - United Nations Recommendations on the Transport of
Dangerous Goods; vPvB - Very Persistent and Very Bioaccumulative

Further information
The above information is believed to be correct but does not purport to be all inclusive
and shall be used only as a guide. The information in this document is based on the
present state of our knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any guarantee of the properties of
the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any
damage resulting from handling or from contact with the above product. See
www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional
terms and conditions of sale.

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