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    Final Report Kimia - Kenneth Olvan Susanto - 23202010009

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    The document discusses a lab experiment on buffer solutions. It includes a table with buffer solutions, their target pH, calculated pH, and volumes used. It also discusses factors that can cause differences between measured and calculated pH values, and how to determine the pKa value of chemical compounds.

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    Final Report Kimia - Kenneth Olvan Susanto - 23202010009

    Uploaded by

    kelvin sentosa
    10 views8 pages
    The document discusses a lab experiment on buffer solutions. It includes a table with buffer solutions, their target pH, calculated pH, and volumes used. It also discusses factors that can cause differences between measured and calculated pH values, and how to determine the pKa value of chemical compounds.

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    Final Report Kimia_Kenneth Olvan Susanto_23202010009

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    The document discusses a lab experiment on buffer solutions. It includes a table with buffer solutions, their target pH, calculated pH, and volumes used. It also discusses factors that can cause differences between measured and calculated pH values, and how to determine the pKa value of chemical compounds.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    10 views8 pages

    Final Report Kimia - Kenneth Olvan Susanto - 23202010009

    Uploaded by

    kelvin sentosa
    The document discusses a lab experiment on buffer solutions. It includes a table with buffer solutions, their target pH, calculated pH, and volumes used. It also discusses factors that can cause differences between measured and calculated pH values, and how to determine the pKa value of chemical compounds.

    Copyright:

    © All Rights Reserved
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    You are on page 1of 8

    Prelab Eksperimen 6

    Buffer

    Laporan Akhir

    Kenneth Olvan.S 23202010009

    DEPARTMENT OF FOOD BUSINESS TECHNOLOGY SCHOOL OF


    APPLIED SCIENCE, TECHNOLOGY, ENGINEERING, AND
    MATHEMATICS UNIVERSITAS PRASETIYA MULYA
    2020

    1.Write down your data on the following table

    calculated
    Buffer pKa target Ph A(mL) B(mL) ph
    glycine-HCl 2.00
    Na citrate-HCl 3.00
    Na citrate-NaOH 5.50
    Phosphate 6.00
    citric acid Phosphate 6.80
    acetate 5.20
    tris maelate 8.20
    Tris -HCl 8.50

    2.Compare the pH of the solutions that you measured with the pH-meter with calculated and target
    pH. Discuss why such difference occurred and identify factors affecting it.
    3.How do you determine pKa of a chemical compound? Explain

    Jawaban:

    1.

    calculated
    Buffer pKa target Ph A(mL) B(mL) ph
    glycine-HCl 2.00 49.3 50.7 2.00
    Na citrate-HCl 3.15 3.00 62.1 37.9 2.99
    Na citrate-NaOH 3.15 5.50 20.8 79.2 5.50
    Phosphate 6.82 6.00 26.1 73.9 6.01
    citric acid Phosphate 6.82 6.80 73.3 26.7 6.80
    acetate 4.76 5.20 14.7 85.3 4.20
    tris maelate 8.06 8.20 25 11.6 8.20
    Tris -HCl 8.06 8.50 25 14.7 7.50
    2. There are many factors that affect the reading from the pH-meter like the value from the pH-meter
    is either higher or lower than the target and calculated value. The difference in result can be cause by
    the purity of the compound itself which makes the buffer more acidic or more basic. Next is the
    temperature in which the buffer solution is made. The temperature of the room may affect the
    production of the buffer solution.

    3. To find the pKa first we must determine which one is the proton donor. When we already know it
    then we must calculate the concentration of the hydrogen ions that is being use. To find the
    n
    concentration of the hydrogen ions we use the formula c= in which n is the number of moles of the
    v
    compound and v is the volume. When we found out the concentration then we use the formula
    Ka=¿ ¿ where A is the concentration of the conjugate base and HA is the weak acid. When we found
    the Ka then we use the logarithm to find the pKa by using pKa=−log Ka . Hence, this how you find
    the pKa of a chemical compound.

    Kalkulasi nomor 1
    Glycine-HCl buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    2.00=2.31+log ¿ ¿
    −0.31=log ¿ ¿
    ¿¿
    Decimal function
    0.4897788194
    ¿ = 0.328759
    1+ 0.4897788194
    1
    [HA]= =¿ 0.67124058
    1+ 0.4897788194
    Molarity
    ¿ = 0.328759 x 0.15 M=0.049314 M
    [HA] = 0.671241 x 0.15 M= 0.100686 M
    Moles
    ¿ = 0.049314 x 0.1 L= 4.9314 x 10−3mol
    [HA] =0.100686 x 0.1 L= 0.0100686 mol
    Total moles = 0.0100686+ 4.9314 x 10−3 = 0.015 mol
    Volume
    ¿ = 4.9314 x 10−3 mol / 0.1M = 0.0493 L Glycine
    [HA] =0.015 / 0.1 M = 0.15 L

    100
    volume of b = −0.0493=0.0507L
    1000

    Na citrate-HCl buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    3.00=3.15+log ¿ ¿
    −0.15=log ¿ ¿
    ¿¿
    Decimal function
    0.707946
    ¿ = 0.414501
    1+ 0.707946
    1
    [HA]= =¿0.585499
    1+ 0.707946
    Molarity
    ¿ = 0.414501 x 0.15 M=0.062175 M
    [HA] = 0.585499 x 0.15 M= 0.087825 M
    Moles
    ¿ = 0.062175 x 0.1 L=0.0062175 mol
    [HA] = = 0.087825 x 0.1 L= 0.0087825 mol
    Total moles =0.0062175 + 0.0087825 = 0.01499999 mol
    Volume
    ¿ =0.0062175mol / 0.1M = 0.0621 L
    [HA] = 0.01499999mol / 0.1 M = 0.1499999 L

    100
    volume of b = −0.0621=0.0379 L
    1000

    Na citrate-NaOH buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    5.50=3.15+log ¿ ¿
    2.35=log ¿ ¿
    ¿¿
    Decimal function
    223.8721
    ¿ = 0.995555
    1+ 223.8721
    1 −3
    [HA]= =4.44697 x 10
    1+ 223.8721
    Molarity
    ¿ = 0.995555 x 0.02 M=0.0199111
    [HA] =4.44697 x 10−3 x 0.02 M= 8.89394 x 10−5
    Moles
    ¿ = 0.0199111 x 0.1 L=0.00199111mol
    [HA] == 8.89394 x 10−5 x 0.1 L= ¿ 8.89394 x 10−6 mol
    Total moles 0.00199111mol +8.89394 x 10−6 = 2.08 x 10−3 mol
    Volume
    ¿ =0.00199111 mol / 0.1M = 0.0199111 L
    [ HA ] = 2.08 x 10−3mol / 0.1M = 2.08 x 10−2 L

    100 −2
    volume of b = −2.08 x 10 =0.0792L
    1000

    Phosphate buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    6.00=6.82+log ¿ ¿
    −0.82=log ¿ ¿
    ¿¿
    Decimal function
    0.151356
    ¿ = 0.13459
    1+ 0.151356
    1
    [HA]= =¿0.86854
    1+ 0.151356
    Molarity
    ¿ = 0.13459 x 0.02 M=0.0026918
    [HA] = 0.86854 x 0.02 M= 0.0173708
    Moles
    ¿ = 0.026918 x 0.1 L= 0.00026918mol
    [HA] = 0.0173708 x 0.1 L= 0.00173708 mol
    Volume
    1
    ¿ =0.00026918 mol / M = 0.00403 L
    15
    1
    [ HA ] = 0.00173708 mol / M = 0.0261 L
    15

    100
    volume of b = −0.0261=0.0739 L
    1000

    Citric acid phosphate buffer


    Concentration of the buffer
    pH= pKa+log ¿ ¿
    6.80=6.82+log ¿ ¿
    −0.02=log ¿ ¿
    ¿ ¿ 0.95499
    Decimal function
    0.95499
    ¿ = 0.488488
    1+ 0.95499
    1
    [HA]= =¿0.511512
    1+ 0.95499
    Molarity
    ¿ = 0.488488 x 0.15 M=0.0732732 M
    [HA] = 0.511512 x 0.15 M= 0.0767267 M
    Moles
    ¿ = 0.0732732 x 0.1 L= 0.00732732mol
    [HA] = 0.0767267 x 0.1 L= 0.00767267 mol
    Volume
    ¿ =0.00732732mol / 0.1M = 0.0732732 L
    [ HA ] = 0.00767267mol / 0.2M = 0.3836335 L
    100
    volume of b = −0.0732732=0.0267 L
    1000
    Acetate buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    5.20=4.76+ log ¿¿
    0.44=log ¿ ¿
    ¿¿
    Decimal function
    2.75423
    ¿ = 0.733633
    1+ 2.75423
    1
    [HA]= =¿0.266366
    1+ 2.75423
    Molarity
    ¿ = 0.733633 x 0.02 M=0.014673
    [HA] = 0.266366 x 0.02 M= 0.053273
    Moles
    ¿ = 0.014673 x 0.1 L= 0.0014673 mol
    [HA] = 0.053273 x 0.1 L= 0.0053273 mol
    Volume
    ¿ =0.0014673 mol / 0.1 M = 0.014673 L
    [ HA ] = 0.0053273 mol / 0.1 M = 0.053273 L
    100
    volume of b = −0.014673=0.0853L
    1000

    Tris-maleate buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    8.20=8.06+ log ¿ ¿
    0.14=log ¿ ¿
    ¿¿
    Decimal function
    1.38038
    ¿ = 0.57990
    1+ 1.38038
    1
    [HA]= =¿0.42010
    1+ 1.38038
    Molarity
    ¿ = 0.57990 x 0.02 M=0.011598 M
    [HA] 0.42010 x 0.02 M= 0.008402 M
    Moles
    ¿ = 0.011598 x 0.1 L= 0.0011598mol
    [HA] = 0.008402 x 0.1 L= 0.0008402mol
    Total moles = 0.0011598+0.0008402 = 0.002 mol
    Volume
    ¿ = 0.0011598mol / 0.1M = 0.011598 L
    [ HA ] = 0.002 mol / 0.1M = 0.02 L
    100
    volume of b = −0.011598=0.0884 L
    1000

    Tris-HCl buffer
    Concentration of the buffer
    pH= pKa+log ¿ ¿
    8.50=8.06+ log ¿ ¿
    0.44=log ¿ ¿
    ¿¿
    Decimal function
    2.75423
    ¿ = 0.733633
    1+ 2.75423
    1
    [HA]= =¿0.266366
    1+ 2.75423
    Molarity
    ¿ = 0.733633 x 0.02 M=0.014673
    [HA] = 0.266366 x 0.02 M= 0.053273
    Moles
    ¿ = 0.014673 x 0.1 L= 0.0014673 mol
    [HA] = 0.053273 x 0.1 L= 0.0053273 mol
    Volume
    ¿ =0.0014673 mol / 0.1 M = 0.014673 L
    [ HA ] = 0.0053273 mol / 0.2 M = 0.0266365 L
    100
    volume of b = −0.014673=0.0853L
    1000

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