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    Midterm Exam (Part 2)

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    Fengari Cresent
    This document contains a 3-part midterm examination on buffers and disinfectants. Part 1 involves calculating the pH of an acetic acid/sodium acetate buffer and the pH after adding NaOH. Part 2 lists the chemical equation for the reaction in Part 1b. Part 3 discusses how 80% ethyl alcohol works as a disinfectant against SARS-CoV-2 by disrupting the lipid structure of viral membranes and denaturing proteins. A figure illustrates alcohol penetrating the viral membrane and coagulating proteins.

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    Midterm Exam (Part 2)

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    Fengari Cresent
    31 views2 pages
    This document contains a 3-part midterm examination on buffers and disinfectants. Part 1 involves calculating the pH of an acetic acid/sodium acetate buffer and the pH after adding NaOH. Part 2 lists the chemical equation for the reaction in Part 1b. Part 3 discusses how 80% ethyl alcohol works as a disinfectant against SARS-CoV-2 by disrupting the lipid structure of viral membranes and denaturing proteins. A figure illustrates alcohol penetrating the viral membrane and coagulating proteins.

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    This document contains a 3-part midterm examination on buffers and disinfectants. Part 1 involves calculating the pH of an acetic acid/sodium acetate buffer and the pH after adding NaOH. Part 2 lists the chemical equation for the reaction in Part 1b. Part 3 discusses how 80% ethyl alcohol works as a disinfectant against SARS-CoV-2 by disrupting the lipid structure of viral membranes and denaturing proteins. A figure illustrates alcohol penetrating the viral membrane and coagulating proteins.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    31 views2 pages

    Midterm Exam (Part 2)

    Uploaded by

    Fengari Cresent
    This document contains a 3-part midterm examination on buffers and disinfectants. Part 1 involves calculating the pH of an acetic acid/sodium acetate buffer and the pH after adding NaOH. Part 2 lists the chemical equation for the reaction in Part 1b. Part 3 discusses how 80% ethyl alcohol works as a disinfectant against SARS-CoV-2 by disrupting the lipid structure of viral membranes and denaturing proteins. A figure illustrates alcohol penetrating the viral membrane and coagulating proteins.

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    CHY 47 

    (BSN 1C) 
    Midterm Examination (Part 2) 

    Answer the following as directed. 


    1. A buffer was prepared by mixing 1.00 mole of acetic acid and 1.00 mole of sodium acetate to
    form an aqueous solution with a total volume of 1.00 liter.    Ka (HC2H3O2) = 1.75 x 10-5 

    a.) What is the pH of the solution? Show your calculations.  5 pts. 

    GIVEN: 1.00 mole of acetic acid


    1.1 mole of sodium acetate
    1.0 liter total volume
    Ka (HC2H3O2) = 1.75 x 10-5 

    SOLUTION:
    Step1. Find pKa Step2. Find pH
    pKa=−log [ ka ]
    pKa=−log( 1.75 x 10−5 ¿ )¿ pH= pKa+log ¿ ¿
    pKa=4.76
    pH=4.76+log ¿ ¿ ¿

    pH=4.76+0

    pH=4.76
    b.) To 500 mL of the buffer solution above, it was added with 30.0 mL of 1.00 M NaOH. What is
    the pH of this solution? Show your calculations. (5 pts.)

    GIVEN: 500 mL of the buffer solution


    30.0 mL of 1.00 M NaOH was added

    SOLUTION:
    Step1. Convert mL¿ L
    1L
    500 mL (
    1000 mL
    =0.5 L   )
    1L
    30.0 mL (
    1000 mL
    =0.03 L   )
    Step2. Get theconcentration
    mol
    C H 3 COOH =
    1
    L ( ( 0.5 )−0.3 )
    =0.887 M
    0.530 L

    −¿=
    (1 molL) (0.5 )+0.3 =1 M ¿
    0.530 L
    C H 3 COO
    Step3. Get the concentration
    pH= pKa+log ¿ ¿
    pH=4.76+log ¿ ¿ ¿
    pH=4.76+0.05
    pH=4.81

    2. Write the chemical equation involved in the chemical reaction of 4b (addition of NaOH). (5 pts.) 

    CH3COOH + OH- CH3COO- + H2O

    3. Discuss briefly the chemistry of proteins for the use of 80% ethyl alcohol as disinfectant in this
    time of pandemic to fight against SARS- CoV 2, the causative agent of COVID- 19.  Show
    illustration(s) where possible.  (5 pts.)

    Alcohols are disinfectants that kill viruses and bacteria, including SARS-CoV-2. To successfully
    combat COVID-19, hand sanitizers must contain at least 80% alcohol (Joshi et al., 2020). It is because
    80 percent solutions allow alcohol to enter the virus or bacterium by disrupting the arrangement of
    the lipids structure of the bacterial plasma membrane, allowing the contents to pour out and
    denature.
    Alcohol is polar and has water-loving hydroxyl groups, and it loves disrupting the protein and
    lipid structures that make up bacterial membranes and viral envelopes. However, in order for this to
    be effective, the alcohol must be in touch with the surfaces for a long enough period of time long
    enough to kill the microorganisms. 20% water content of 80% solution slows evaporation, therefore
    increasing the surface contact time and enhancing the effectiveness as water functions as a catalyst
    and plays a vital role in denaturing the proteins of vegetative cell membranes. 80 percent ethyl
    solution on the other hand, disrupts the usual arrangement of the lipid structure of the plasma
    membrane by poking holes in the microorganism and breaking the intramolecular hydrogen that
    holds portions of the protein in folded shape. Eventually, it will penetrate the entire cell, coagulating
    all proteins and killing the microorganism (Figure 1).

    Figure 1. Mechanism of Ethyl Alcohol against SARS-CoV-2

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