The document describes a student's lab report on pipetting techniques. It details the steps performed, materials used, potential issues, and parts of a micropipette. Proper pipetting is important to avoid contamination and ensuring accurate volumes are measured.
The document describes a student's lab report on pipetting techniques. It details the steps performed, materials used, potential issues, and parts of a micropipette. Proper pipetting is important to avoid contamination and ensuring accurate volumes are measured.
The document describes a student's lab report on pipetting techniques. It details the steps performed, materials used, potential issues, and parts of a micropipette. Proper pipetting is important to avoid contamination and ensuring accurate volumes are measured.
The document describes a student's lab report on pipetting techniques. It details the steps performed, materials used, potential issues, and parts of a micropipette. Proper pipetting is important to avoid contamination and ensuring accurate volumes are measured.
Program of study/Year:Biotechnology Course instructor:Mrs Emrira Hodo
Name\Last name: Afesa Krakulli
Topic:Pipetting
Lab Report
Describe all the steps you performed during the experiment, when pipetted a specific liquid. List the order in which the component’s reaction are added into a tube.
1:On the first day of lab our professor presented us a power point about pipeting. On those slide we learned basic information about pipeting. 2:Firstly,in the lab we need it to put on safety equipment and cleaned our work space and pipets with 70% ethanol. 3: After I took the micropipete,I adjusted it based on the tip I was going to use. 4:I took the device in my hand, I opened the box of the tips and put the micropipette above one of them and pressed into it until the tip was firmly attached. 5:I put the plunger into its first stop and dipped inside the test tube which contains liquid.After that,I released the plunger calmly with the tip still in liquid. 6: Then I inserted the tip into the second test tube, which is empty.i close the test tube without touching the interior. 7: Lastly, I cleaned the micropipette with 70% ethanol and put the pipette back to its place
What did you learn during this session? What are some basic rules during pipetting in laboratory, which you would advise others to consider?
In this laboratory lesson I managed to learn about different types of pipetts. I had the opportunity to learn how to properly connect the tip the pipette. One of the most important rule about the laboratory is that we should have a clean and non contaminated environment. Another rule is to keep the pipette as vertical as possible.
- List key materials to complete the required experiment.
1. Micropipette ( 0,5 to 10 ul, I used 10 ul)
2. Micropipette tips (10ul) 3. Water 4. 2 test tubes ( one with the water, one without) 5. Safety equipment ( lab coats, gloves) 6. 70% ethanol
Troubleshooting; if something goes wrong during a lab experiment, could it be because of
pipetting technique? If yes, describe how?
Most of the time it can happen because of some issues during the lab experiment like:
Forcing the solution out of the pipet causes too much to be delivered.
1. Using a dirty pipet causes too little or contaminated solution to be delivered. Leaving little droplets behind on the walls (except for the small amount in the tip) causes too little solution to be delivered.
2. Forcing the pipet into the bulb usually causes liquid to be sucked into the bulb. This contaminates the bulb which must then be thoroughly rinsed and dried.
3. Allowing the tip of the pipet to rise above the liquid in the container usually causes the liquid to be sucked into the pipet bulb.
4. A broken or chipped pipet can reduce the amount of liquid held after transfer. This causes too much liquid to be delivered.
List the main parts of a micropipette and write the respective function.
Plunger: The topmost part of the micropipette. Pushing down the plunger helps in the aspiration and dispensation of the desired amount of liquids. It consists of two stops, the first stop is for aspiration and the second stop is for dispensation.
Tip ejector button: A button beside the plunger helps in the removal of pipette tips.
Volume adjustment knob: It is a knob attached to the plunger. Turning it helps in adjusting the desired volume in the adjustable volume micropipette
Digital volume display window: It displays the volume that the micropipette can withdraw.
Plastic shaft: A cylinder structure filled with air in air micropipette.Once you press the piston ,some amount of air from the shaft releases helping in the aspiration of the liquid. In contrast releasing the piston helps dispence the liquid and the air again fills up the shaft.
Ejector arm: It is an extension of the tip ejector button. It puts pressure on the pipette cone once the ejector button is pressed.
Tip cone: It is the place where the pipette tip is attached.
Pipette tip: It is equipment made up of plastics that come in direct contact with the liquid.
