Bedienungsanleitung Quanta Lyser 3000 - 0

Inova Diagnostics, Inc.
QUANTA-Lyser®
3000/4000
Operators Manual
IMPORTANT: Please read carefully before operating the
QUANTA-Lyser instrument.
9900 Old Grove Road
San Diego, CA 92131
United States of America
Technical Service (U.S. & Canada Only):+1-877-829-4745
Technical Service (Outside the U.S.): +1-858-805-7950
support@inovadx.com
Release Information
Inova Diagnostics reserves the right to make technical improvements to this equipment and documentation
without prior notice as part of a continuous program of product development. Information in this document is
subject to change without notice.
Copyright © 2019 Inova Diagnostics, Inc. All rights reserved. This document is the copyright of Inova
Diagnostics, Inc. and must not be copied or reproduced in any form without prior consent.
Page 2 QUANTA-Lyser Operators Manual Rev.4

QUANTA-Lyser 3000/4000
Operators Manual
IMPORTANT: Please read carefully before operating the QUANTA-Lyser 3000/4000 instrument.

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About CH. 1
Table of Contents
Table of Contents ............................................................................................................ 5
CHAPTER 1: About .................................................................................... 7
1.1 This Manual............................................................................................................ 7
1.2 Policy Statement.................................................................................................... 7
1.3 Symbols and Conventions .................................................................................... 7
1.4 Intended Use .......................................................................................................... 9
CHAPTER 2: Preinstallation and Installation Procedures .................... 11
2.1 Preinstallation Procedures ................................................................................. 11
2.2 Requirements for Personnel ............................................................................... 11
2.3 Requirements for Installation ............................................................................. 12
2.4 Installation ........................................................................................................... 13
2.5 Customization and Training................................................................................ 13
2.6 Assay Menu ......................................................................................................... 13
2.7 Labeling and Orientation Precaution ................................................................. 13
2.8 System Requirements ......................................................................................... 14
CHAPTER 3: Principles of Operation...................................................... 17
3.1 System Capabilities ............................................................................................. 17
3.2 Hardware Components ....................................................................................... 18
3.3 Software Features ............................................................................................... 32
CHAPTER 4: Performance Characteristics and Specifications ........... 33
4.1 Physical Attributes .............................................................................................. 33
4.2 Performance Specifications................................................................................ 34
CHAPTER 5: Operating Instructions...................................................... 37
5.1 System Start-Up................................................................................................... 37
5.2 Conditioning and Maintenance Actions ............................................................. 40
5.3 Performing an Analytical Session ...................................................................... 41
5.4 Pausing and Aborting a Session ........................................................................ 78
5.5 Low Liquid Errors ................................................................................................ 80
5.6 Short Sample Handling ....................................................................................... 81
5.7 Working with Analytical Results and Reports ................................................... 85
CHAPTER 6: Calibration Procedures..................................................... 93
CHAPTER 7: Potential Hazards .............................................................. 95
7.1 Introduction ......................................................................................................... 95
7.2 Potential Safety Hazards ..................................................................................... 95
CHAPTER 8: Service and Maintenance .................................................. 99
8.1 Introduction ......................................................................................................... 99
8.2 Daily Maintenance ............................................................................................. 100
8.3 Weekly Maintenance ......................................................................................... 102
8.4 Preventative Maintenance 1 .............................................................................. 103
8.5 Preventative Maintenance 2 .............................................................................. 104
8.6 System Liquid and Buffer Handling ................................................................. 104
8.7 Waste Handling.................................................................................................. 115
8.8 Opening the Front Shield Manually .................................................................. 117
8.9 Exchanging Tips ................................................................................................ 119
8.10 Handling Instrument Data ................................................................................. 122
QUANTA-Lyser® Maintenance Record.................................................. 123
CHAPTER 9: Troubleshooting............................................................... 125

CHAPTER 1: About
1.1 This Manual
This manual is intended to be a reference guide for trained operators and laboratory personnel. It
provides detailed information about operation and maintenance of the QUANTA-Lyser instrument
family.
The images used in this manual may not represent the specific instrument, component, or
software/firmware version being used. Options and accessories may or may not be illustrated in
each figure.
Illustrations based on CAD drawings may not show cables and tubing.
Carefully read all relevant chapters before use.
1.2 Policy Statement

It is the policy of Inova Diagnostics to improve products as new techniques and components
become available. Inova reserves the right to change specifications at any time.
Inova Diagnostics is committed to providing customer with inventive, high quality products and
services that are environmentally sound.
1.3 Symbols and Conventions

The following symbols are used on instruments, modules, and manuals.
Whenever these symbols appear on instruments or modules, please observe appropriate safety
procedures.
Caution
Consult the manual for further information, proceed with caution
Caution
Consult the manual for further information, proceed with caution
Caution
High voltage, possibility of electric shock, proceed with caution
Caution
Risks associated with a laser beam
Caution
Touching this part while not properly grounded may cause damage due to electrostatic
discharge (ESD)
Caution
Risks associated with biohazards

Caution
Risks associated with chemicals or biological materials
Caution
Risks associated with flammable materials
Caution
Risks associated with puncture hazard
Caution
Advice associated with the use of lubricants
Caution
Risks associated with heavy load
Caution
Do not walk or stand here
Note
Must be performed by certified engineers only
Note
Consult the manual, proceed with caution (label on instruments only)
Note
Advises special attention
Tip
Useful Hints
Note
RoHS aims at restricting hazardous substances in electrical and electronic equipment (EEE)
so as to contribute to the protection of human health and the environmentally sound recovery
and disposal of electrical and electronic equipment waste.
Note
WEEE Directive 212/19/EU: Waste Electrical and Electronic Equipment (WEEE) legislation
lays down collection, recycling, and recovery targets for electrical goods.
Note
Environmental Protection Use Period – 25 years: The period during which toxic or hazardous
substances or elements contained in electronic information products will not leak or mutate
Manufacturer
Indicates the manufacturer
Note
Catalogue or Part Number

Note
Batch Code
Note
Date of Manufacture
Note
European Authorized Representative
Note
European Conformity
Note
In Vitro Diagnostic Medical Device
Note
Consult Instructions for Use
1.4 Intended Use

The QUANTA-Lyser Operators Manual is designed to assist in training and operation of the
QUANTA-Lyser instrument family.
QUANTA-Lyser instruments are fully automated benchtop processors for use in conjunction with
certain reagents to measure a variety of analytes. QUANTA-Lyser is intended to automate slide and
reagent barcode reading, sample dilution and distribution, reagent pipetting and dispensing, slide
and plate washing, dispensing slide mounting medium in preparation for microscope analysis and
photometric measurement of microwell plates.
QUANTA-Lyser systems provide flexible automation solutions for mid-to-high volume laboratories, a
broad test menu, and fast turnaround time. The QUANTA-Lyser 4000 supports high volume
Immunofluorescence Assay (IFA) processing. The QUANTA-Lyser 3000 is an Enzyme-linked
Immunosorbent Assay (ELISA) and IFA assay processor.
For further information, please contact instrument supplier.


Preinstallation and Installation Procedures CH. 2
CHAPTER 2: Preinstallation and Installation Procedures

2.1 Preinstallation Procedures
This chapter contains information for preinstallation and setup of the QUANTA-Lyser instrument.
Note
Installation must be performed by certified engineers only.
Before installing the QUANTA-Lyser, laboratory personnel, in tandem with authorized service
personnel, should inspect the site to identify a desirable location and ensure that the environment
meets installation requirements. The following checks should be made:
• Receiving area and transportation pathway
• Adequate access to loading dock
• Facilities to offload the crate from the truck
• Space available to uncrate
• Adequate doorway width
• Safe and adequate passage
• Working area
• Ambient conditions
• Electrical power requirements
2.2 Requirements for Personnel

This section covers general competence and training necessary for transportation, installation, use,
maintenance, and servicing of QUANTA-Lyser instruments.
Type of Task Personnel Training and Experience
Transportation No special requirements No special requirements
Certified service engineers authorized
Technically skilled, with a good
Installation and qualified to install and maintain the
knowledge of the application field
entire robotic system
Appropriately trained and
experienced personnel, familiar
Routine Use (Running pre- with use of computers and
Laboratory technicians or equivalent
programmed test sequences) automation in general.
Familiarity with MS Windows®
environment
Programming and optimization High degree of knowledge of the
Inova certified personnel only
of test sequences relevant application field
Certified service engineers authorized
Technically skilled with a basic
Preventative Maintenance and qualified to install and maintain the
understanding of the application
entire robotic system
Certified service engineers authorized Background and experience in
Servicing and qualified to install and maintain the electronics/mechanics with
entire robotic instrument computer knowledge
2.3 Requirements for Installation
Caution
Do not install the power cable yet. To avoid potential electrical shock and/or damage to the
instrument, do not install the power cable before a certified service engineer has completed
the setup procedure.
Whenever the instrument is moved from a cold place (e.g. transport at temperature below
freezing point) to room temperatures, there is a chance of condensation. Allow 48 hours after
transport before the instrument is put into operation.
2.3.1 Site
The QUANTA-Lyser instrument must be placed on a sturdy workbench, preferably equipped with
wheels and fixing brakes. For service and maintenance, the instrument must be accessible from all
sides. A clearance of at least 1 m (3 feet) must be provided. The instrument dimensions listed in
Chapter 4: Performance Characteristics and Specifications do not take into account handles used to
lift the instrument. If an immobile work bench is used, there must be enough room for 4 movers to
lift the instrument onto the counter (instrument may not be placed directly beside a wall).
The instrument must not be located near a heat souce, ventilation, air conditioner, or exposed to
direct sunlight. Make certain that the waste container can be placed within 1.5 meters (5 feet) of the
instrument. Note that the unit has a gravity-fed waste unit that must be placed somewhere
underneath the instrument, not to one side. The instrument should be close to an AC power outlet.
The power cord reach is 6 feet.
2.3.2 Mains Supply

The mains supply for the entire instrument should be voltage regulated, properly grounded and
surge protected.
The QUANTA-Lyser instrument has a self-regulating power supply. See “Electrical and
Communication” section for power supply specifications.
Caution
Please read carefully.
To protect operating personnel, the National Electrical Manufacturers’ Association (NEMA)
recommends that the instrument is correctly grounded. This instrument is equipped with a 3
conductor power cable that, when connected to an appropriate AC power outlet, grounds the
instrument. To ensure that this protection feature functions properly , do not operate the
instrument from an AC power outlet that does not include a ground connection.

2.4 Installation
Note
Installation must be performed by certified engineers only.
The QUANTA-Lyser system is delivered as an integrated unit. The system is installed and
assembled by trained engineers. Installation consists of the following steps:
• Remove packaging and check for damage
• Verify that all components against the component list supplied in the box
• Remove all foam blocks from the moveable parts (i.e. robotic arms)
• Raise the instrument from the base and install on the work table (bench)
• Verify that the electrical plug conforms to the electrical technical specifications
• Install the software and connect the USB dongle
• Proceed with setup, calibration, and verification of the deck and robotic arms
2.5 Customization and Training

Following installation, each system is customized and verified by an Inova field service
representative or an international service specialist. On-site training is performed after installation.
2.6 Assay Menu

The menu of assay protocols supporting kits supplied by Inova is installed with QUANTA-Lyser
software. The QUANTA-Lyser is an open system and is capable of running most commercial
ELISA/EIA and IFA assays. These can also be programmed for the QUANTA-Lyser assay menu.
Such assays should be confirmed and validated by the user.
2.7 Labeling and Orientation Precaution

To ensure correct barcode scanning, labels must be attached according to the specification below.
Observe the orientation of tubes and bottles in the racks.
2.7.1 120 mL Becker Vial Labeling

For diluents, labels must be peeled off original reagent container and adhered to the 120 mL Becker
vial at the position shown in the figures below. The distance between the vial bottom and label
bottom must be 17 mm, which is equivalent to the 20 mL mark on the 120 mL Becker vial scaling.

17 mm
Figure 2-1: Reagent Label & Diluent Bottle Figure 2-2: Correct Label Placement
2.7.2 Orientation of Bottles in Racks

The orientation of barcodes within the rack is very important. The barcode should be centered in its
rack position. There is a notch in the rack to show the center point of that position.
Figure 2-3: Incorrect Bottle Orientation Figure 2-4: Correct Bottle Orientation
2.8 System Requirements
2.8.1 Space Requirements

The QUANTA-Lyser system is comprised of the following components: a bench-top analyzer,
computer, liquid supply unit (integrated on QUANTA-Lyser 4000 model), waste container and an
uninterrupted power supply (UPS) unit. The liquid supply unit is positioned to the right of the
instrument and holds system liquid and wash buffers. A 10 liter waste container is located
underneath the instrument. If the instrument is placed on a bench rather than a moveable cart, be
sure to provide ample space behind the instrument for repair and routine maintenance activities.
Please see “Physical Attributes” section for model specific instrument dimensions.
2.8.2 Power Requirements

AC power cords are supplied with the instrument according to international standards. Appropriate
AC power cords and plugs are included in the shipment inside the component boxes. Uninterrupted
power supply (UPS) unit prevents problems due to power surges and is required. It is
recommended that the instrument is shut down when not in use.

2.8.3 Water Supply, Waste, and Drainage
Depending on model, the system is equipped with either 5 or 9 L System Liquid bottle(s) and two or
four 2 L wash buffer bottles. Deionized water is used to prepare System Liquid and Wash Buffers.
Tip and plate/slide wash waste is collected in a 10 L waste container. The liquid waste container
can be disconnected from the system and emptied manually. Alternatively, the waste can be
connected through a drain line directly to the laboratory drain. Empty reagent vials, dilution tubes,
microtiter plates and slide should be discarded appropriately according to laboratory procedures.


Principles of Operation CH. 3
CHAPTER 3: Principles of Operation

QUANTA-Lyser incorporates state of the art modern electronics, robotics, and computing
components. The hardware and software components of QUANTA-Lyser are customized and
configured to automate ELISA/EIA and IFA assays.
3.1 System Capabilities
3.1.1 Instrument Capabilities

QUANTA-Lyser hardware components provide barcode reading, pipetting, washing, mixing,
incubation and detection methods in compliance with ELISA/EIA and IFA requirements:
• Automatic sample, reagent, and IFA slide barcode reading
• Preparation of sample dilution with flexible volume options
• Preparation of serial dilutions
• Sample distribution to various configurations of wells on glass slides and 96-well microtiter
plates
• Reagent addition
• Microtiter plate washing
• IFA Slide well washing
• Mixing in microwell tubes and microtiter plates
• Liquid sensing in system wash containers
• Temperature controlled incubation
• Detection of optical densities utilizing single/dual wavelength options
3.1.2 Software and Applications

QUANTA-Lyser software controls all stages of the automatic processing phases. It provides a user
interface for configuration of the instrument in the most effective mode and includes an extensive
menu of assay definition options that allows single application optimization.
The flexibility of the QUANTA-Lyser provides advanced options for ELISA, EIA and IFA processing:
• Multiple assays combined onto a single plate frame
• Custom racks that fit Inova kit reagents
• Various bottles sizes can be used
• A variety of washing methods can be developed and programmed
• Multiple types of plates and slides are supported
• Various options to perform qualitative and quantitative data analyses are available
• Pipetting error handling and logging
• Connection to LIS
3.2 Hardware Components
The primary robotic components of QUANTA-Lyser are pipetting and tool arms, barcode reader, tip
washing station, EIA plate washer module, EIA absorbance reader, deck (or workspace) with flexible
sets of racks that configure the QUANTA-Lyser workspace layouts.
3.2.1 Hardware Devices
3.2.1.1 Pipetting Arm and Tips

The QUANTA-Lyser pipetting arm is configured with four pipetting and two aspiration tips (present
on some models). Tips are aligned along the Y-axis of the pipetting arm and each tip has an
individual Y- and Z- motor and liquid level detector. FlexiSpan refers to the independent Y-spacing
of the tips. Tips can access nonconsecuitve wells or tubes in a column and simulateneously detects
different levels in various containers.
Figure 3-1: Pipetting Tips
Liquid Detection (LD)

The QUANTA-Lyser detects liquid level by monitoring the change in capacitance (electrical charge)
between each tip and instrument deck. Each tip assembly is equipped with a liquid sensor, which
enables it to detect liquids (except non-polar substances) upon contact. The liquid level detector
monitors changes in capacitance between pipette tip and deck. When the pipette tip touches a
liquid surface, the sudden change in capacitance generates a detection signal.
The LD mechanism design minimizes influence from the surrounding environment. Liquid level
sensors are programmed to detect insufficient amounts or total absence of sample and reagent
liquid. The system will notify the user by alarm so that corrective steps can be taken.
Submerge Level
The liquid submerge depth of the pipette tip during sampling can be controlled to minimize cross-
contamination (usually 1 – 2 mm).
Note

An error may occur if the tip touches the side of a vessel or air bubbles even if the contact is
insufficient to cause capacitance change. Since the liquid level detector cannot identify what
material causes a change in capacitance, it is imperative that the tips do not touch any surface
except the liquid to be detected.
Poor water quality and the use of unproven additives in the system liquid can affect the
performance of the liquid detection.
Pumps and Valves

Pipetting pumps (micro-annular gear pumps) are used to pipette samples, reagents, diluents, and
wash buffers for IFA assays. Pumps in the robotic arm, reduce the need for syringe pumps and
extended lengths of system liquid tubing. QUANTA-Lyser pumps operate on positive displacement
and are programmed to dispense precise volumes ranging from a few microliters to 1mL.
A single pump is dedicated to one tip channel and controlled by the software. Valves are in line
with the pump and controlled automatically by firmware.
Liquid flows in channels featuring multiple liquids such as system liquid and wash buffers. System
liquid and buffers are controlled by Valve Unit 101 located on the back of the X-rail.
Liquid that will be removed through the aspiration tip is aspirated by the corresponding pump of the
Pump Unit 101 located behind the rear rail of the instrument.
Figure 3-2: Pumps and Valves

Figure 3-3: QUANTA-Lyser 4000 Hydraulic Diagram
Figure 3-4: QUANTA-Lyser 3000 Hydraulic Diagram (Model Dependent)
3.2.1.2 Pipette Tips Wash Station and Waste Station

Pipette tips are thoroughly washed between steps with system liquid containing 0.4% Contrad 70
detergent solution in deionized water. Washable tips are rinsed under program control at the wash
station. This module features a raised cup for each tip, into which the tip enters for washing.
System liquid uses distilled or deionized water with 0.4% Contrad 70 detergent agent. When the
tips are positioned in the cups, system liquid is aspirated by the pump and forced through each tip.
The flow is activated by the conical shape of the cup and washes the outside of the tip in an upward
motion before spilling into the moat and down the drain. Both inside and outside of each tip are
thoroughly cleaned in a single step. A tube moves waste fluid from the drain to a waste station
placed below the instrument.
In a second step, the pipetting tip dispenses system liquid while the aspiration tip aspirates to wash
the internal channel.
Figure 3-5: Pipette Tips Wash/Waste Station
3.2.1.3 Tool Arm and Handler (Gripper)

The QUANTA-Lyser instrument is equipped with an independent tool arm with a barcode reader
(BCR) tool and handler tool, as well as gripper fingers, on certain models (QUANTA-Lyser 3000).
The BCR can be swiveled by 90 degrees and rotated by 270 degrees to read 2D barcodes
positioned horizontally or vertically. The handler transports microtiter plates between deck positions
and modules. The handler is controlled by its own Y- and Z- motors, and can rotate. This function
improves access to modules and accessories situated at any position in the rear or front of the
instrument.
The tool arm and the BCR are controlled by their own X-, Y-, and Z-motors. This allows the BCR to
read 2D barcodes on reagent bottles and slides.

Figure 3-6: QUANTA-Lyser 4000 Tool Arm Figure 3-7: QUANTA-Lyser 3000 Tool Arm
Barcode Readers
An LED barcode reader is configured to read 1D barcode labels on sample tubes.
Figure 3-8: Sample Barcode Scanner
An additional barcode reader, a moveable camera to read 2D barcodes on IFA slides, diluent
bottles, reagent bottles and standard/control bottles, is located on the tool arm (pictured above).
The BCR tool is able to swivel down to read reagent barcodes from the side, and back up to read
barcodes on slides located in slide trays seated on the decktray.

3.2.1.4 ELISA Plate Washer
The ELISA plate washer washes 8 wells simultaneously using 8 coaxial probes and 4 wash buffer
lines in a 96 well plate format. The washing protocol and wash buffer lines can be defined and
programmed, including number of wash cycles, volumes, and timing specifications.
Figure 3-9: ELISA Plate Washer Module
Flat-bottom, C-bottom, U-bottom, and V-bottom microtiter plates can be accomodated. The vertical
positions of the probes can be programmed to an accuracy of 0.1 mm. The wash head moves both
vertically and horizontally, aspirating liquid from plates during the wash process.
3.2.1.5 Incubator
An optional four position incubator is available. The incubator incubates microtiter plates at 37oC
without shaking. Incubation temperature and duration can be specified within the assay definition.
Figure 3-10: Incubator Module
3.2.1.6 Absorbance Reader

The absorbance reader module (mounted below the deck with an opening for top access) is
configured with 5 wavelength filters (405 nm, 450 nm, 492 nm, 550nm, 620nm) and is suitable for
the most common EIA assays.
Figure 3-11: Absorbance Reader Module
3.2.1.7 System Liquid Detection Unit (QUANTA-Lyser 3000)

The liquid detection unit includes a capacitive level sensor that indicates the presence of fluid. It
does not detect volume of fluid, only that some is present at the start of the run. It is the operator’s
responsibility to ensure sufficient wash volume is present. The software will not allow for a run to
begin unless fluid is present.
Figure 3-12: QUANTA-Lyser 3000 System Liquid Detection Unit

3.2.1.8 Control and Liquid Supply Unit (QUANTA-Lyser 4000)
Figure 3-13: QUANTA-Lyser 4000 Control and Liquid Supply Unit
The liquid supply unit inlcudes a drawer where system liquid containers and buffer bottles are
placed. The liquid level in each container and bottle is monitored by weight using a load cell under
each container (weight measuring).
To prevent particles from entering the pump, a filter is installed on all system liquid and buffer tubing.
The computer, mounted above the liquid station drawer, is controlled by the height-adjustable touch
screen monitor or an external keyboard and mouse situated in the keyboard drawer under the
instrument.
3.2.1.9 QUANTA-Lyser Deck Workspace

QUANTA-Lyser holds patient sample tubes, reagents, standards, controls, and consumables on the
deck workspace. Resa-trax sit on the deck and make up a portion of the deck workspace. Resa-
Trax are tracks that are compatible with sample and reagent racks and allow for all liquid to remain
secure as well as ensuring the precise location of each position. Sample tubes are placed in sample
racks which are then loaded onto the Resa-trax 12S. Reagent bottles are loaded on the reagent
racks which are then loaded onto Resa-Trax 601 or 901 (depending on instrument model). Deep-
well dilution blocks are used to prepare single dilutions or multiple titers from patient samples and
are placed onto specific deck locations as indicated by the software.

Resa-trax 12S
Figure 3-14: Resa-Trax 12S
Primary patient sample tubes are loaded onto a Resa-trax 12S. Resa-trax 12S is a semi-automated
barcode reading system that uses 12 racks with 20 positions in each rack.
Sample tubes (outer diameter 12 – 16 mm) with barcodes are placed in racks, with barcodes facing
the fix mounted barcode reader (BCR). Each rack is manually inserted into it’s respective slot and
sample barcodes are scanned as the sample tube passes the sample barcode reader. Most
common laboratory barcodes can be read.
NOTE: QUANTA-Lyser 4000 has two Resa-trax 12S on its deck.
Resa-trax 601 (On QUANTA-Lyser 4000) and Resa-trax 901 (On QUANTA-Lyser 3000)
Figure 3-15: Resa-Trax 601 Figure 3-16: Resa-Trax 901

Resa-trax 601 provides 6 positions (slots; in terms of application = strip) featuring various rack sizes
for tracking identification barcodes by the barcode reader tool (the Resa-trax itself does not have a
reader). Resa-trax 901 is idential with the exception of having 9 positions.
Bottles and tubes with barcodes are placed in racks and manually inserted into a slot. Diluent
Racks use 3 positions each, Reagent Racks use 2 positions each, and Control Racks use 1 position
each. The barcode reader tool automatically starts reading as soon as the front shield is lowered.
3.2.1.10 Front Shield

The Front Shield encloses the workspace. The shield must be closed during operation. To open, lift
the handle until fully open. The shield will remain in this position until it is closed. To close, push
down on the handle until fully closed.
Figure 3-17: Front Shield
Caution
Pinching hazard. Be sure that your hands and fingers are clear when closing the front shield.
3.2.1.11 Controls and Connectors
Mains Switch, LED Mains, USB Ports

Emergency Button
Figure 3-18: QUANTA-Lyser 4000 Controls and Connectors

Mains Switch, LED Mains
Emergency Button
Figure 3-19: QUANTA-Lyser 3000 Controls and Connectors
Computer Start Button

(QUANTA-Lyser 4000 Only)
LED Mains
Mains Switch
Figure 3-20: Mains Switch, LED Mains, Computer Start Button

Figure 3-21: Front USB (QUANTA-Lyser 4000 Only)
Mains Switch
Powers the robot on and off
LED Mains
Lit LED indicates instrument power is on
Computer Start Button (QL 4000 Only)
Power the computer on. To shut down the computer, use the Windows operating system
Front USB Ports (QL 4000 Only)
Two USB 2.0 connectors (e.g. to connect a memory stick, external hard disk, handheld barcode
reader, etc.)
Figure 3-22: Emergency Button

Emergency Button
Switches robot off immediately (does not shut down computer on QL 4000, robot only)
Caution
Any work in progress will be lost after pushing the emergency button.
Rear Side
Figure 3-23: QUANTA-Lyser 4000 Rear View
Figure 3-24: QUANTA-Lyser 3000 Rear View

Figure 3-25: Mains Socket
Figure 3-26: Rear LAN Port and Rear USB (QUANTA-Lyser 4000 Only)
Mains Socket
Mains inlet, situated behind the rear rail
Rear LAN Port (QUANTA-Lyser 4000 only)
To connect the instrument to a local area network (LAN)
Rear USB (QUANTA-Lyser 4000 Only)
3.2.2 Monitor (QL 4000 Only)

The monitor is height-adjustable (range 200 mm / 8 inch) and can be tilted horizontally and vertically
by approximately 30 degrees.
Figure 3-27: QUANTA-Lyser 4000 Monitor
Monitor Controls
The monitor controls are located on the rear, either vertically on the left hand side or horizontally on
the top side (depending on monitor version):
Power: Switches monitor on and off
LED: Lit LED Indicates monitor is powered on
Menu: Opens On Screen Display (OSD ); used to confirm the selected setting
SL+: To navigate up
SL-: To navigate down
Auto: Resets the monitor to factory settings
3.3 Software Features

QUANTA-Lyser software controls the QUANTA-Lyser ELISA/IFA processing system. The software
provides the interface for system configuration, programming assay protocols, creating, scheduling
and running an analytical session, interpretation of results, data storage, and reporting.

Performance Characteristics and Specifications CH. 4
CHAPTER 4: Performance Characteristics and Specifications

4.1 Physical Attributes
4.1.1 Dimensions and Weights

The instrument’s shipping crate physical dimensions and weight:
Instrument Model Length Depth Height Weight

QUANTA-Lyser 420 kg
81” 42” 51”
4000 926 lbs
QUANTA-Lyser 320 kg
59.1” 41.4” 47.6”
3000 705 lbs
The instrument’s physical dimension and weight, uncrated, are:

Height Height
Instrument Model Width Depth Weight
(Hood Closed) (Hood Open)
QUANTA-Lyser 39” (Including 292 kg
71.5” 38” 53.5”
4000 Monitor) 644 lbs
QUANTA-Lyser 210 kg
45.3” 32” 36.6” 52”
3000 462 lbs
4.1.2 Environmental and Operating Conditions

Feature Specification
Operating Conditions Temperature: 15 - 30ºC / 60 - 85ºF
(Up to 2000 m above sea level) Relative Humidity: 25 – 85% at 30ºC / 85ºF (non-condensing)
Temperature: 10 - 40ºC / 50 - 104ºF
Storage Condition
Relative Humidity: 30 – 80% at 30ºC / 85ºF (non-condensing)
Temperature: 0 -60ºC / 32 - 140ºF
Transport Conditions
Relative Humidity: 30 – 80% at 30ºC / 85ºF (non-condensing)
Noise <85 dBA (QUANTA-Lyser 4000) / <80 dBA (QUANTA-Lyser 3000)
IP Code 20
4.1.3 Electrical and Communication

110 – 240 VAC 50 – 60 Hz 850 VA
Mains Input
220 VAC 60 Hz 850 VA
Mains Distribution Unit 101: F10 A / 250 V (2x, P, and N)
Mains Fuses
PSU 208: F6.3 A / 250 V (2x, P, and N)
Arm(s): F8 A / 250 V
Output Fuses High Power: F8 A / 250 V (2x)
CAN-bus Loop: F3.15 A / 250 V
CAN-bus (internal)
Communication
Gigabit Ethernet (external)
Installation Category II

4.1.4 Sample and Plate Format
Plate Size SBS Format
Slides 75 x 25 x 1 mm (W x D x H)
Minimum OD 12 mm
Sample Tube Size
Maximum OD 16 mm
4.1.5 BCR Tool

Reading Process Manual rack load, automatic barcode reading
CODE39, ITF, Industrial2of5, COOP2of5, Codabar, CODE128,
Barcode types
GS1128, GS1 DataBar, CODE93, EAN/UPC, Trioptic Code39
QR, MicroQR, DataMatrix, PDF417, MicroPDF, MaxiCode, GS1
2D-Code Types
Composite
Maximum Barcode Length Depends on barcode type
Barcode: 31 – 97 mmv(cell size 0.339 mm)
Reading Distance
2D-code: 29 – 106 mm (minimum line width 0.339 mm)
Reading Resolution 0.127 mm
4.2 Performance Specifications
4.2.1 Capacity
QUANTA-Lyser 3000 can process batches of up to 240 samples, using EIA or IFA protocols.
QUANTA-Lyser 4000 can process batches of up to 480 samples, using IFA protocol only.
4.2.2 Quality Control

QUANTA-Lyser is capable of processing control material.
4.2.3 System Characteristics

Minimum sample/reagent volume: 5 µl
Volumes Maximum sample/reagent volume: 1 ml (Sample), 2mL (Reagent)
Minimum detectable volume: 150 µl
Washable Pipetting Tips 4 (liquid channel 1 – 4)
2 (liquid channel 1 and 2)
Aspiration Tips
Available only on models with IFA capability.
Dilutions, single and multistage Yes
QUANTA-Lyser 3000 QUANTA-Lyser 4000
Sample Batch Size 240 480
QUANTA-Lyser 3000 QUANTA-Lyser 4000
Maximum Number of IFA Slides 30 40
Number of Control Positions 40 20
Number of Reagent Positions 24 12

Number of Diluent Positions 7 7
Number of Dilution Wells 480 672
Maximum Number of Wash ELISA: 4
2
Buffer Lines IFA: 2


Operating Instructions CH. 5
CHAPTER 5: Operating Instructions

5.1 System Start-Up
• Switch the instrument on by pressing the mains switch.
• Double click QUANTA-Lyser Software Icon ( ) to start the software.

o Login screen is displayed.
Figure 5-1: Login Screen
• Enter user name and password into text fields, then click Login to log into QUANTA-Lyser
software.
o The home screen will be displayed.
5.1.1 Common Buttons

There are a series of buttons at the top of the screen which are repeated on most other screens.
These buttons are:
Button / Element Description
Returns to Worklist screen from any other window
Returns to Timeline screen when a run is active

Returns to the Home screen
Opens Maintenance screen
Communications Icon – Indicates status of communications

module (see below for details)
5.1.1.1 Communications Icon

The Communication Icon changes to a variety of colors to reflect different communication scenarios.
Along with different colors, there may also be badge(s) present to reppresent messages or requests
downloading. See below chart for details of the Communications Icon options.
Button / Element Color Description Badge Description
Communications module not

1 message waiting to be read
enabled
Communications established 1 message waiting to be read
1 message waiting to be read

Communications established 10 requests downloading (note that
there may be multiple tests per request)
Communications module enabled

but not established
Communications module
connecting

5.1.2 Home Screen
Figure 5-2: Home Screen
Note
If Laboratory Information System (LIS) is configured and available, the connection is
established automatically.
The color of the communications icon indicates whether the connection is enabled or disabled
and whether the connection has been established or not. See “Communications Icon” section
for more detailed information.
Unique Elements of the Home Screen

Creates a new worklist (see “Creating a Worklist” section)
Opens Assay List screen where all assays programmed on the

system can be seen and edited (with appropriate permission
level for user that is logged in)
Opens Results List screen where a list of completed sessions
can be found and reviewed and reports can be generated and
printed
Opens Settings screen where hardware and software settings

are controlled
Logs current user out and returns to Login screen

Shuts down QUANTA-Lyser software
5.2 Conditioning and Maintenance Actions

At startup, the liquid channels must be conditioned by filling the buffer lines (Prime), Decontaminate
tips, as well as a flush with system liquid (Flush). Individual actions may be completed by clicking
the corresponding button or all three functions can be performed by clicking Daily button.
Figure 5-3: Maintenance Screen
Unique Elements of the Maintenance Screen

Performs flush, decontaminate and prime procedures
Flushes system liquid channels thoroughly
Performs decontamination procedure
Primes wash buffer lines

Allows wash programs to be tested
Closes the window
5.3 Performing an Analytical Session
5.3.1 Wash Buffer Assignment

Prior to setting up a worklist, the position(s) of the wash buffer(s) required for the a run must be
established.
1. From the Home screen, click the Settings button to proceed to the settings menu.
2. Click on the Robot button from the menu on the left side of the screen.
3. Click on the Wash Buffers button to proceed to the Wash Buffers setup screen.
Figure 5-4: Robot Settings Screen
4. Once in the Wash Buffers screen, configure the wash buffer(s) to be used during the run being
scheduled.

Figure 5-5: Wash Buffers Setup Screen
Wash Buffer can be selected from the liquids list and the wash line must be indicated. Note that IFA
wash buffers must only be placed in bottles connected to buffer lines C and D, while ELISA buffer
may be placed in any buffer bottle. Indicate the bottle location corresponding to the need for the
buffer. For example, IFA wash buffer will only be used by Tip Washer as seen in the PBS II
example in the image above. ELISA wash buffer is configured to use the plate washer line.
5.3.2 Creating a Worklist

From the Home screen, click the Worklist button to proceed to the Worklist screen.

Figure 5-6: Worklist Screen
Unique Elements of the Worklist Screen

Clears existing worklist
Adds samples to the worklist
Adds assays to the worklist
Allows sample barcodes to be scanned
Number buttons to the right correlate with sample racks
Allows the worklist to be printed
Opens a window that displays a legend

Proceeds to assay setup
1. From the worklist screen, click the Add Samples button.
Figure 5-7: Adding Samples to Worklist
2. Enter the number of sample tubes to be scanned, enter a sample naming prefix (if running
without barcodes), and verify sample tube size.
a. Click the Submit button to return to Worklist screen.
3. If using barcoded samples, click the Scan button to open the Scan Racks window.
a. For automatic barcode scanning, verify that all necessary sample racks are selected and
click Automatic scan.

Figure 5-8: Scan Racks Window
b. Click the Automatic Scan button and follow the prompts to scan sample racks.
Figure 5-9: Scanning Sample Racks
c. If barcode IDs are to be entered manually, click the Manual Scan button to proceed to the
Manual Sample Identification screen.
Figure 5-10: Manual Sample Identification Screen
d. In this screen, barcode IDs can be manually entered in the text boxes on the right of the
screen or the Skip button may be selected for sample tubes to be skipped.
e. In the case of a duplicate barcode, the software will notify that duplicate barcodes have
been detected and will give the option to select one of the duplicate barcode positions to
be used.

Figure 5-11: Duplicate Sample Barcode Notification
f. Once a selection is made, a confirmation window will appear.
Figure 5-12: Duplicate Sample Selection Confirmation
g. Finally, the software will indicate that all errors have been resolved and the unused
duplicate position must be skipped.

Figure 5-13: Duplicate Sample Selection Completion
h. Once returned to the worklist screen, all barcode IDs and locations will be listed, with the
skipped duplicate position being blank.
Figure 5-14: Worklist Following Duplicate Sample Selection Completion
i. If a barcode is unreadable, the following screen will appear, giving the opportunity to

manually enter the barcode ID, skip the sample, or Rescan.
Figure 5-15: Unread barcode
4. If connected to an LIS, the LIS will be queried as sample racks are scanned and the worklist
will automatically be populated with assays and sample selections.
Figure 5-16: Worklist Screen After Sample Barcode Scanning

a. During test download, information may not appear on screen until all orders for all tests in
the rack have been received in QUANTA-Lyser.
b. To see existing messages, select the communications icon ( )
Figure 5-17: Communications Log
c. To see the detail of the message, select the mouse ( ) icon.
Figure 5-18: Communications Log Details

5. If an LIS is not connected, assays and dilution information must be selected manually.
a. Click the Add Assay button, select the assay(s) to be run, and click the Submit button.
Figure 5-19: Assay List
b. The worklist is updated to include the assay(s) that have been selected.
Figure 5-20: Worklist Following Assay Selection

c. Sample dilutions now must be selected. Click on each assay name at the top of the
worklist screen to open the Dilution Selection window for that particular assay.
d. For IFA assays, depending on specific assay configuration, multiple dilution options may
be available. Dilutions can be selected and applied to all samples by clicking the All
Samples button or apply only to specific samples by clicking the Range button.
Figure 5-21: IFA Dilution and Sample Selection
e. For ELISA assays, the dilution factor is hard coded into each assay protocol so the only
options are to select all samples in the worklist to be run on the assay in question or to
select a range of samples.

Figure 5-22: ELISA Sample Selection
f. A message will appear confirming that the selection is correct.

6. The worklist is now complete and ready for review.
Assay Name(s)
Sample Barcode/ID
Sample Rack Number

Sample Position Within Rack
Sample IFA Dilution Factor
Multiple IFA Dilution
Selection # of Dilutions
Multiple IFA Dilution

Selection Highest Dilution
Sample ELISA Selection
Unselected Sample
Figure 5-23: Completed Worklist
7. A Worklist Report may be printed, if desired, by clicking the Print icon in the Worklist screen.

Figure 5-24: Worklist Report Example
8. Upon completion of the worklist, the Proceed button on the Worklist screen will become active
to allow for reagent and consumable loading.
Figure 5-25: Proceeding After Worklist Completion
9. If assays in the worklist contain reagents that are configured to be lot specific, an additional
window will pop up to allow the user to enter the expected lot number for each liquid that is
configured to be lot specific.

Figure 5-26: Liquid Lot Identification Window
5.3.3 Assay Setup

The Assay Setup screen has multiple tabs for different material that must be loaded to the
instrument deck. Highlighted buttons indicate an action is required for that material before the run
may be started.
There are a series of buttons that are common to most tabs and screens of the Assay Setup screen.
Tabs which require action will have be highlighted in orange. The common buttons for the Assay
Setup screen are:
IFA Slides Tab manages slide requirements, the triangle icon at the
bottom of the button indicates this is the active tab
Predilutions Tab manages predilution block requirements
Liquids Tab manages kit reagents requirements
Wash Buffers Tab manages wash buffer requirements
When an alert is present, the Close button is active and may be

clicked to remove the alert from view

Returns to Worklist screen
Becomes active once all required components are loaded and will
proceed to the timeline screen
5.3.3.1 IFA Slides Tab (Active only on IFA Runs)

The IFA Slides tab contains information about slide type and quantities required for the run. IFA
Slides Tab also allows the user to scan slide barcodes using the automated barcode scanner or
enter slide barcode or identifying information manually. The presence of an orange alert message
at the top of the screen indicates either that slide barcodes have not been automatically scanned or,
if automatic scan is not being used, the slide carrier has not been marked as loaded.
Information in this tab is used to place IFA slides into the appropriate slide tray and ensure those
trays are placed in the appropriate position on instrument deck.
Figure 5-27: IFA Slides Tab
Unique Elements of the “IFA Slides” Tab

Slide Tray number, if highlighted, it is required for current run;
triangle icon at the bottom of the button indicates active
selection

Slide Trays that are not currently selected and not needed for
current run
Allows user to automatically scan barcodes for required

slides
10. Slide barcode or other identifying information must be entered.

a. If slides with 2D barcodes are being run, the automatic scan function may be used:
b. Review the needed slides by clicking on each slide tray number. Review both slide type as
well placement in the rack, place slides in rack(s) and place them in the appropriate
position on the deck.
c. Click the Scan Slides button. The Scan Slides window will appear.
Figure 5-28: Scan Slides Window
d. Select the slide tray/carrier to scan or click the Select all carriers button to select all.
e. Click the Scan Slides button to begin the automatic barcode scanning process.
f. Upon completion, the slides displayed in the IFA Slides tab will contain images of correct
barcode numbers and the Carrier has been loaded check box will be automatically
selected.
g. If automatic scanning is not being used or if barcode or other slide identifier is being input
manually:
h. Slide barcode or other identifying information (i.e. slide number if writing manually on a

slide, etc.) can be entered in the text field below each slide. Enter information, then click
enter to apply.
Select once slide tray

has been loaded onto
instrument deck
Slide barcode ID or other

identifying information is
entered here
Figure 5-29: Scan Slides Window Details
i. Once all slides have been identified, if desired, and slide tray is physically loaded onto the
appropriate position on the instrument deck, click the Carrier has been loaded box,
indicating that the slide tray has been placed on the deck.
j. Repeat for any other slide trays needed on the run.
k. If a slide barcode is duplicated, a notification will appear on the affected slides and the text
field below will remain active to allow for a new barcode entry.

Figure 5-30: Duplicate Slide Barcodes
l. If a slide barcode is not recognized, a notification will appear, indicating there is no barcode
assigned to the affected slide(s). The text field below the slide will remain active to allow
for a new barcode entry.
Figure 5-31: Unread Slide Barcode

Figure 5-32: Incorrect Slide Type
m. If an incorrect slide barcode type is used (i.e. the system is expecting an HC barcode prefix
for the selected assay and instead a slide with an EC barcode prefix is scanned), a Prefix
Error label will appear on the affected slide(s). The text field below the slide will remain
active to allow for a new barcode entry.
5.3.3.2 EIA Plates Tab (Active Only on ELISA Runs)

The EIA Plates tab contains information about the number and type of ELISA plate strips that are
required for the run. Clicking on plate numbers using the buttons on the left will show the layout for
each plate frame that is to be placed on the instrument deck as well as location of control vs. sample
wells on the strips and assay name. Required plate type is also listed. Multiple ELISA assays may
be combined onto one plate frame if they have identical assay steps, incubation times, and the
same wash buffer.
Information in this tab is used to place ELISA plate strips into the appropriate plate frame and
ensure those plates are placed in the appropriate position on instrument deck. Toggling back and
forth from one plate number button on left side of the screen to another will display different plate (if
multiple plates are required based on the scheduled worklist).

Figure 5-33: EIA Plates Tab
Unique Elements of the EIA Plates Tab

Plate number, triangle icon at the bottom of the button indicates
active selection
Plates that are not currently selected do not contain triangle icon
5.3.3.3 Predilutions Tab

The Predilutions tab contains information about the number and location of predilution blocks that
are required for the run. Clicking on predilution block numbers using the buttons on the left will
show which predilution racks and wells are scheduled to be used during the predilution step of the
run.

Figure 5-34: Predilutions Tab
Unique Elements of the Predilutions Tab

Predilution block information, triangle icon at the bottom of the button
indicates this is the active selection
Predilution blocks that are not currently selected do not contain
triangle icon
Resets predilution blocks, assumes user is discarding any partially
used predilution blocks from the deck and is placing fresh blocks
5.3.3.4 Liquids Tab

The Liquids tab contains information about samples, diluents, controls, calibrators and reagents that
are required for the currently scheduled worklist. Liquid type, liquid name, lot number (If a specific
lot number is required due to a lot specific reagent) and volume needed for each liquid is listed in the
table. To change which liquid types are being displayed, click the buttons above the table. A
triangle icon at the bottom of a button indicates that liquid type is being displayed in the table.
Caution
Check bottles containing reagents and eliminate air bubbles on the liquid’s surface to
prevent liquid level sensing problems.

Figure 5-35: Liquids Tab
Unique Elements of the Liquids Tab

Displays Sample information, a triangle icon at the bottom of the
button indicates this fluid type is selected and is being displayed
Displays required Diluent information, a triangle icon at the bottom of

the button indicates this fluid type is selected and is being displayed
Displays required Control information, a triangle icon at the bottom of

the button indicates this fluid type is selected and is being displayed
Displays required Calibrator information, a triangle icon at the bottom
of the button indicates this fluid type is selected and is being
displayed
Displays required Reagent information, a triangle icon at the bottom
of the button indicates this fluid type is selected and is being
displayed
Allows user to enter kit lot number(s), if desired
Allows user to automatically scan reagent barcodes
1. Review the list of materials needed and load into appropriate reagent racks.
2. If desired, click the Kit Lot button to proceed to Kit Lot screen and record kit lot numbers to be
included in results report.

Figure 5-36: Kit Lot Window
3. Click the Scan Liquids button to proceed to automatic reagent barcode scanning window. The
table lists all required liquids, required lot number (for lot specific reagents), volume needed
and volume loaded onto the system. Make note the color of the fluid number on the left side of
the table. Red highlighting indicates that there is an insufficient amount of that particular
reagent or that the reagent hasn’t been scanned or manually identified yet.

Figure 5-37: Automatic Reagent Scan Window
Unique Elements of the Automatic Reagent Barcode Scanning Window

Cancels the reagent scan process and returns to the previous screen
Opens a window that displays a legend
Becomes active upon completion of reagent scanning, returns to

previous screen
Restarts the entire reagent scan process
Rescans the last rack that was scanned
Allows for bottle position to be manually programmed rather than 2d

barcodes being automatically scanned to identify positions
a. For automated barcode scanning, follow on-screen prompts to remove all reagent racks
and load them one at a time, starting with the Diluent rack on the left, followed by the
reagent racks and then standard/control racks.
b. It is important to load racks as indicated on the screen. Failure to load racks in the correct
position or order could result in a hardware collision when the gripper arm with the barcode
scanner moves over to the rack position to read the barcodes.

c. To manually identify the position of all diluents, reagent and control bottles within the racks,
click the Manual button to proceed to the manual reagent identification screen.
d. Click on a rack position that corresponds to actual bottle placement to indicate the location
of each bottle:
Figure 5-38: Manual Reagent Selection Window
e. Enter barcode (Full GTIN information) or click the Choose button to proceed to a pop up
window containing information for each possible liquid associated with the currently
scheduled worklist.

Figure 5-39: Liquid Selection Window
f. Click the arrow to the right of the desired selection to place that bottle in the selected
position.
Figure 5-40: Liquid Following Manual Selection
g. Repeat for remaining liquids.

h. Click the edit ( ) button in the Actions column to edit the lot information for that
particular liquid. Lot number and expiration date may be entered. Any information entered
here will appear on the Results Report.
Figure 5-41: Liquid Lot Specific Information
4. Once all reagents have been automatically scanned or manually entered, the table in the liquid
screen will list the volume scanned. This volume is determined according to the number of
bottles that were identified and their recorded fill volumes. Green highlighting of reagent
numbers indicates sufficient volume has been loaded. Red highlighting indicates additional
bottles of that reagent are required.

Figure 5-42: Completed Liquid Identification
a. The edit buttons in the Actions column allow for additional reagent information (such as lot
number or expiration date) to be added
b. Clicking on a reagent position in the diagram of the reagent racks will select that bottle.
Information for the selected bottle is displayed above the table. Clicking the Empty button
beside the bottle information will remove that bottle from display and assume the bottle has
been physically removed from the rack.
c. The Legend button will open the Legend window explaining which statuses are possible to
be seen in this window.
d. Once all needed reagents are placed, click the Complete button to proceed.

Figure 5-43: Liquids Tab Legend
5.3.3.5 Wash Buffers Tab

The Wash Buffers tab contains information about the wash buffers that are planned to be used for
the currently scheduled worklist. The buffer description, assays in which it is used, assigned plate
(for ELISA) or tip (for IFA) wash line, as well as the liquid identifier code, is listed in the table. The
buffer bottles are placed into the appropriate position(s) in the liquid detection unit (see “Wash Buffer
Assignment” section). The liquid detection unit includes a capacitive level sensor that indicates the
presence of fluid. It does not detect volume of fluid, only that some is present at the start of the run.
It is the operator’s responsibility to ensure sufficient wash volume is present.
If insufficient fluid is present, or necessary buffer has not be assigned during Wash Buffer
Assignment procedures, an error will appear in the Wash Buffers tab.

Figure 5-44: Wash Buffers Tab
Unique Elements of the Wash Buffers Tab

Allows the instrument to recheck the signal from the sensor from the
System Liquid Detection Unit
If all necessary wash buffers are present and liquid is found with the level sensor, no error will
appear and the Wash Buffer tab will simply display the location and status of each buffer.

Figure 5-45: Wash Buffers Tab Without Errors
5.3.3.6 Timeline Screen

Once all required materials have been loaded onto the instrument and the timeline screen is active,
the run is ready to be started. Timeline(s) can be reviewed, zoomed in or out or specific
plates/carriers aborted. A list of steps is also available for review. When ready, click the Start Run
button to begin.

Figure 5-46: Timeline Screen Prior to Start of Run
Unique Elements of the Timeline Screen

The zoom sliding scale can be clicked and dragged to increase or
decrease the zoom of the timeline
Displays Timeline, triangle icon at bottom of button indicates an
active selection
Displays run Steps, triangle icon at bottom of button indicates an

active selection.
Aborts the indicated ELISA plate or Slide Tray/Carrier
Returns to the Material Setup screen
Begins the run
Only active after session has begun; pauses the instrument
Once the run has begun, the Timeline screen will adjust slightly as the run proceeds, as needed, to
reflect accurate timeline.

5.3.4 EIA Read
If it is necessary to re-read an ELISA plate, it is possible to perform a Read Only session that will
perform a read step only to a plate that has already been processed. It is important to verify the
plate had been processed correctly prior to setting up a Read Only session.
1. From the Worklist screen, click the EIA Read button.
Figure 5-47: Worklist Screen EIA Read
2. A pop up window appears and will give options to use the Current Worklist or recently
completed sessions that had been processed on that system.
Figure 5-48: Read Only Screen
a. Current Worklist will utilize the assay and sample selections present in the worklist screen.
b. Choosing a recent session will assume all calibrator/control/sample locations from that
particular session.
3. After making a selection, click the Recover Worklist button.

Figure 5-49: Recover Worklist
4. The Worklist screen will now have a Read Only button in place of the Proceed button. Click
the Read Only button.
Figure 5-50: Read Only
5. The assay setup screen will appear. Confirm the EIA Plate placement on the instrument deck
aligns with the information in the EIA Plates tab and click the Schedule button.

Figure 5-51: Schedule Read Only
6. All plates from the worklist will be present on the timeline screen. Abort any plates that do not
need to be read by clicking the red X beside that plate number and proceed with the read by
clicking the Start Run button.
Figure 5-52: Read Only Timeline

7. A confirmation message will appear to confirm the plate(s) have been placed in the correct
positions. Click Review Setup to return to the previous screen for additional review or Start
Run to begin the session.
Figure 5-53: Read Only Confirmation
a. Upon completion of setup review, if applicable, click the  button to return to the Timeline
screen.
8. After completion of the Read Only session, results can be accessed from the Results List.
Please note that the Results Report will indicate that a Read Only session has been
performed.
Figure 5-54: Timeline Screen Prior to Start of Run
Note
Original session results will remain in the Results List in addition to the Read Only
session.
Any notes on samples from the original session, such as over incubation, manually done,
etc. will not be carried over. The original session results should be referenced to check
for any exceptions.
If a recent session was selected from the Read Only screen, any
reagent/control/calibrator lot infromation from that session will carry over. If a Read Only
session Is generated from a new worklist, no lot information will be captured.
If an assay’s steps are modified in any way, any recent session containing that assay will
be removed from the Read Only windows.

5.4 Pausing and Aborting a Session
Figure 5-55: Pausing a Session
While a session is running the Pause button is active in the Timeline screen. In the case of an error,
the system will automatically pause and automatically restart following the resolution of the error. If
the operator chooses to pause, they may do so by clicking the Pause button.
Note
Upon clicking the Pause button, the system will wait for a suitable time to pause.
Once a suitable pause time is reached, the front cover will unlock.
Caution
Excess pause time may negatively impact any tests in process.

Figure 5-56: Session Paused
Once the system is in a paused state, the operator may select one of the following options:
• : The buttons beside each row of the timeline will allow for that particular Slide
Carrier/ELISA Plate to be aborted. Note that if multiple assays are shared on one slide tray
or ELISA Plate, they would all be aborted. There is no way to abort individual assays on a
shared tray/plate. A message will appear confirming that the operator wants to abort the
slide carrier/plate. Upon completion of the abort action, the system returns to the Timeline
screen and will remain paused until the operator chooses Resume.
Figure 5-57: Carrier Aborted Figure 5-58: Plate Aborted
• Abort: The entire session will be aborted if this option is chosen. A message will appear
confirming that the operator wants to abort the session. Depending on the step in the
process when the session is aborted, the probes may not be properly cleaned. If sample
predilution or distribution steps were in process, it is recommended to perform
Decontamination in the maintenance screen. If mounting medium was being dispensed, it is
recommended to perform the Long Flush in the maintenance screen. If there is uncertainty
of the step in the process, it is recommended to run both the Decontamination and Long
Flush prior to the Abort Session.
Figure 5-59: Abort Session Message
• Resume: Allows the session to immediately resume where it left off.
5.5 Low Liquid Errors

A number of errors may be present during a run for a number of reasons. It is important to note the
error, preferably including a screen shot, when reaching out to your service provider for support.
One common error that may be seen if insuffient liquid volume is found and will be accompanied by
an acoustic alarm, is a low liquid error.
Figure 5-60: Low Liquid Error
The error message will indicate which tips were trying to aspirate the liquid as well as the position of
the bottle. The operator may select one of the following options:
• Retry: The tip will attempt to detect the liquid level again and aspirate the liquid, if sufficient
volume is now recognized.
• Abort: The abort option will abort the entire session.
• Ignore: The tip will move to the z-max (position programmed as the bottom of the container)
position and aspirate without performing additional level sensing. If sufficient liquid is
present, it will be aspirated. If no liquid is present, air will be aspirated.
• Refill: This option allows the operator to refill the bottle(s) in question. Upon choosing this
option, the pipette tips will return to the wash station, the door will unlock, and the user will
be allowed the refill the liquid in question.

Figure 5-61: Refill Option
5.6 Short Sample Handling

In the case of a low liquid for samples, there are two possible scenarios for short sample handling.
These options are defined in the instrument settings during instrument installation.
5.6.1 Basic Short Sample Handling

If Basic short sample handling is configured, a low liquid message will appear with each low liquid
detection:
Figure 5-62: Basic Short Sample Handling
The operator may select one of the following options:

• Process Later: The system will continue to dilute the remaining samples. At the end of the
Predilution step, another warning message will appear to allow the user to handle all short
samples by either manually diluting or skipping the sample (see “Short Sample Processing”
section for more details).
• Retry: The tip will attempt to detect the liquid level again and aspirate the liquid, if sufficient
volume is now recognized.
• Abort: The abort option will abort the entire session.
• Ignore: The tip will move to the z-max (position programmed as the bottom of the container)
position and aspirate without performing additional level sensing. If sufficient liquid is
present, it will be aspirated. If no liquid is present, air will be aspirated.
5.6.2 Advanced Short Sample Handling

If Advanced short sample handling is configured, all sample predilutions will be completed. At the
end of the predilultion process, the Low Liquid to process warning will appear (see “Short Sample
Processing” section for further details).
5.6.3 Short Sample Processing
At the end of the predilution process, an alert will appear, indicating that there are low liquids which
need to be processed.
Figure 5-63: Low Liquids to Process
The operator may select one of the following options:

• No: The Timeline screen will appear and the system remains paused until the operator
selects the Dilution Map button.
Figure 5-64: Dilution Map Button
• Yes: Advances to the Dilution Map screen.
5.6.3.1 Dilution Map Screen

Figure 5-65: Dilution Map Screen
The Dilution Map screen allows for any short samples to either be completed manually or skipped.
Low liquid samples are highlighted in red.
1. The user must choose for each low liquid sample whether to Skip or Complete(Manually).
Once an action is selected, click Next to proceed.
a. Skip: If Skip is selected, the screen will indicate such. All predilution operation
regarding that sample will cease. The session will continue and skipped samples will
be indicated as such in the Results and if it is to be run on an ELISA assay, no data
reduction will be done for that sample.

Figure 5-66: Skipped Sample
b. Complete (Manual): If the Complete(Manual) option is selected, instructions are given

to indicate the volume of sample to be aspirated and where it should be dispensed, to
allow the operator to perform the dilution manually. A remark will be added to the
Results indicating that the sample was manually diluted.
Figure 5-67: Complete (Manual) Sample

5.7 Working with Analytical Results and Reports
Final test results are automatically calculated (for ELISA) and available immediately upon
completion of an analytical session. Analytical results can be released to the LIS system and
printed. Quality Control requirements are checked and flagged in case of a failure. Pipetting
problems that occurred during an analytical session that may impact results are documented in the
run report.
5.7.1 Accessing Results

Following the completion of a run, a pop up window will allow the user to access run results.
Alternatively, results may be accessed at any time by accessing the Results List from the Home
screen.
Figure 5-68: Results List
The Results List contains a list of all runs on the system. The list may be sorted by using the Filter
button. Information about LIS status, and QC criteria pass or failure for ELISA results can be seen
at a glance on the Results List. To view details about any run, click the icon beside the desired
session.

5.7.1.1 IFA Results
Figure 5-69: IFA Results : Slide Layout Tab
Figure 5-70: IFA Results : Lot Information Tab
Detailed information for an IFA session includes a graphical display of the slides, sample barcode
ID, and dilution in the slide layout teb. Lot specific information about the controls and reagents used

during the run can be found in the Lot Information tab.
Clicking the Send to LIS button will send all information to the LIS, even if it has been previously
sent.
5.7.1.2 ELISA Results
Figure 5-71: ELISA Results: Summary Tab
The Summary tab contains basic session information (dependent on data reduction type), data
reduction formula, QC Checks, Mehtod type, calculation date, Units, Kit Lot number (if entered by
user), and a list of the Result Frames used for interpretation of each sample result.

Figure 5-72: ELISA Results: List Tab
The List tab contains a table of results and includes the ID, OD, Value, Average OD, Average Value,
CV %, Interpretation, and Remarks (includes notes about low liquid errors, extended pauses, etc).
Figure 5-73: ELISA Results: Plate Layout Tab

The Plate Layout tab includes a graphical display of the plate as well as OD information. The
Actions colum has a series of check boxes. This allows for an individual replicate to be disabled and
the data recalculated. If a replicate is deselected (to disable), a warning message will appear to
confirm the decision. The data reduction is recalculated upon confirmation.
Note
Modification to results will be flagged on the final report.
Figure 5-74: ELISA Results: Plot Tab
The Plot tab contains curve data for ELISA assays with curve data reduction type selected. The
curve type (Method) as well as Plot Type (axis) can be adjusted, and will result in a recalcuation of
the data. Upon changing either selection, a warning message will appear, confirming the decision to
change. The data reduction is recalculated upon confirmation.
Note
Modification to results will be flagged on the final report.

Figure 5-75: ELISA Results: Lot Information Tab
Lot specific information about the controls and reagents used during the run can be found in the Lot
Information tab.
Clicking the Send to LIS button will send all information to the LIS, even if it has been previously
sent.
5.7.1.3 Printing Results Report

To print the results report, simply click the printer icon at the bottom of the screen at any time while
reviewing run results details. A message will appear, confirming the print:
Figure 5-76: Printing Report
Upon confirmation of othe print request, a print preview screen will appear:

Figure 5-77: Results Report Screen
The Results Report screen is a print preview of the selected session results. Using the Fit H and Fit
V button will zoom the preview. The Previous and Next buttons can be used to toggle from one
page to another. The Save button will allow for the report to be saved as a PDF. The Print button
will proceed to the Print Options window:
Figure 5-78: Print Options
Simply select the desired printer, paper size and number of copies and click Submit to begin the
printing process.
5.7.1.4 Export OD for ELISA Results

To export ODs of a given ELISA assay, click the Export OD button at the bottom of the screen at
any time while reviewing run results details. A windows explorer window will appear, allowing the
file name and location to be defined. A .csv file will be generated and saved in the selected location.
Upon opening the .csv file in Excel, raw ODs for primary, secondary (if used) and subtracted OD (if
both filters are used) can be seen.
Note: Export OD functionality is only available for assays that have both data reduction and QC
rules applied.
Figure 5-79: Example OD Export .csv

Calibration Procedures CH. 6
CHAPTER 6: Calibration Procedures

Note
QUANTA-Lyser calibration procedures are performed by certified service engineers.
Calibration and functional tests verify the correct operation of the instrument after maintenance
and service procedures and investigate suspected malfunctions of the instrument.
Calibration procedures are performed during installation, annual preventative maintenance and
following repaired or replacementof parts and modules.
The procedure involves calibration of the instrument layout through the use of a two point deck
calibration as well as the option to utilize offsets of individual racks or objects to ensure correct
pipetting and/or gripper positioning.
If problems with calibration is observed, please contact Inova Technical Support or your local
support provider.


Potential Hazards CH. 7
CHAPTER 7: Potential Hazards
7.1 Introduction
Additional safety measures and precautions are required if the instrument is used for applications
involving infectious, toxic, explosive, flammable, and/or hazardous substance.
Such additional measures are the complete responsibility of the operator or the supplier of the
reagent package.
For further information, please contact you instrument supplier.
Please read this section and the “Potential Safety Hazards” section before operating the instrument.
Operators must be certified in general laboratory safety practices and the specific safety
requirements of their QUANTA-Lyser instrument. If the equipment is used in a manner not specified
by the manufacturer, protection provided by the equipment may be impaired.
QUANTA-Lyser is a robot that can be programmed to automatically perform chosen tasks. The
open geometry of the system facilitates the loading of predilution blocks, samples, reagents, plate,
and/or slides prior to commencement of the run and their off-loading after the run has been
completed. Should user intervention be necessary during a run, the program should be interrupted
strictly according to the instructions given in this manual and/or displayed on the screen of the PC.
7.2 Potential Safety Hazards
7.2.1 Mechanical
Caution
QUANTA-Lyser is a robotic device that operates under computer control. As with most robotic
devices, there is a potential for injury and bodily harm from moving mechanical components
whenever the instrument is in operation.
The hazard is minimized by the use of encoders on each movement axis, which immediately
detects incorrect positioning when a power overload occurs due to a physical blockage.
The instrument is designed for automatic “hands-off” operation only. The housing prevents
accidental contact with moving parts. The front shield must be closed whenever the instrument
is running.
Never reach or lean into the instrument’s working area when the unit is in an operating mode,
unless the system has paused and displays a dialog box indicating that user-intervention is
required. Reaching onto the deck at any other time during a run may result in injury to the user
and/or damage to the instrument and/or an aborted run.
Read the error warnings provided by the software in case of malfunction and choose the
corrective action carefully.
Discontinue use if the instrument emits noise above normal levels.

7.2.2 Electrical
Caution
Standard electrical safety precaution should be applied.
Whenever the instrument is moved from a cold place (e.g. transport at temperature below
freezing point) to a room temperature, there is a chance of condensation. Allow 48 hours after
transport before the instrument is put into operation.
Do not operate the instrument from an AC power outlet that has no ground connection.
Do not touch the switches or outlets with wet hands.
Switch the instrument off before unplugging the power cable.
Unplug the instrument prior to cleaning up any major spills and prior to servicing any of the
electrical or internal components.
Only qualified personnel should perform electrical servicing.
Do not connect any external devices to the power supply.
7.2.3 Precautions for Use

Caution
Please respect GLP (good laboratory practice) at all times.
Interruption of a run can adversely affect the assay in process.
All functions performed within the context of preparing, performing, and completing a run
should be done with caution and care and with general respect both to the
instrumentation and to associated chemicals, samples, and other devices.
Misuse of the robotic system is hazardous to the user.
Broken glass can result from accidental misuse (dropping bottles during service, etc.) or
from instrument malfunction, e.g. an arm crash during faulty test sequence.
Never perform any manual tasks on the instrument deck while the instrument is operating.
Always make certain that the instrument is disabled before reaching into the work surface
to add or replace items.
Never operate the instrument with the front shield open.
Take care not to splash or spill liquids when manually performs a task on the work
surface. Immediately clean up accidental spills and/or splashes.
In case of a crash, immediately clean all related components if they came in contact with
any liquids.
Defects in a motor or a power driver might cause uncontrolled XYZ-movements on the
instrument, which could cause a crash and may harm the user.
The QUANTA-Lyser instrument is heavy. There is a risk of back injury if the device is
moved.
(For QUANTA-Lyser 4000 model only) The monitor protrudes the instrument. Mind your
head. Take care during transport (doorways).
Waste container may contain biohazardous liquid.

Avoid direct sight to the laser beam.
The robotic system is approved to handle substances with flash point above 70ºC, the
instrument must be placed on a fire resistant surface with nonflammable material above it,
and operated in a fire protected environment.
The instrument can cause injury, even if the instrument is not in operation.
There is risk of injury caused by reflex actions occurring due to unexpected movement or
instrument malfunction.
The robotic arm can exert enough force to be a pinch hazard.
The pipette tips are sharp enough to be a puncture hazard.
Watch your fingers while closing front/rear door, lowering the front shield/rear panel, or
sliding the monitor (QUANTA-Lyser 4000 model only). There is a risk of pinching.
7.2.4 Instrument Safety

Caution
It is important that the QUANTA-Lyser deck is setup in the exact manner describes and
that no unnecessary items be present on the deck during operation.
The liquid waste container must be emptied at least once a day, or as it becomes full.
Ensure that the tubing extending from the instrument to the liquid waste container does
not have any kinks and that there are no loops in the tubing path that would prevent the
waste solution from flowing freely downwards.
Depending on the installation configuration, cut the waste tubing if it is too long to allow
proper gravity drainage.
Ensure that the tubing from the system liquid container is free of kinks.
Ensure that the system liquid container is adequately filled prior to the start of each run.
The QUANTA-Lyser instrument should be connected to an uninterruptible power supply
(UPS). This will allow a run to continue in the event of a power interruption and will
prevent potential damage to the instrument due to loss of power during a run.
Inadequately tightened liquid connections might cause leaks.
Blocked tips or obstructed fluid lines might cause leaks.
In case the instrument is switched off (by the mains switch, emergency button, or power
blackout) there is a chance that the BCR tool moves downward (Z-axis). Manually move
the tool upwards before powering the instrument on.
Do not use a lifting device (e.g. crane trolley) to lift the instrument using its handles. Refer
to the QUANTA-Lyser Service Manual.
An inaccurately programmed sequence on the instrument might cause a crash.
Whenever programming a sequence, including single tests and scripts, do a mock run
first.
Do not touch the tip, liquid level detector housing, Z-rack, or housing unless you are
grounded properly. Electrostatic discharge may reset boards, which leads to the method
being aborted.

Do not walk or stand on the balance pan.
Do not put a heavy load on the liquid station or keyboard drawer (both found on
QUANTA-Lyser 4000 model only).

Service and Maintenance CH. 8
CHAPTER 8: Service and Maintenance
8.1 Introduction
The QUANTA-Lyser instrument is designed to require a minimal amount of maintenance by the
user. However, to preserve the accuracy and reliability of the instrument, the following maintenance
procedures must be performed on a regular basis according to the maintenance schedule below
(Maintenance intervals are based on the assumption of 2 full instrument runs per day 250 days per
year). Please note that instruments with higher throughput or use of some reagents may require
more thorough or more frequent maintenance.
The Maintenance Operation window can be accessed by clicking on the Maintenance icon, which
can be found in the upper right corner of most screens.
The Maintenance Operation window contains a series of different actions that can be performed.
Figure 8-1: Maintenance Operation Window
Daily Maintenance
Daily maintenance is performed by the operator at the beginning and end of each day (or after 8
hours of operation, whichever comes first) and takes approximately 15 minutes.
Weekly Maintenance
Weekly maintenance is performed by the operator at the end of the week and takes approximately
30 minutes.
Preventative Maintenance 1 (Every 6 Months)
Preventative Maintenance 1 (PM1) is performed by the operator every 6 months and takes
approximately 10 minutes.

Preventative Maintenance 2 (Annual)
Preventative Maintenance 2 (PM2) is performed by a certified service engineer every year and takes
approximately 4-8 hours.
A maintenance record sheet is provided for verification of maintenance. Whenever maintenance

actions are performed, the form should be updated.
It is important to maintain this record, as repairs necessitated by misuse, abuse, or negligence are
not covered under the warranty or service contract.
Instrument data, including the run list, should be backed up at least weekly, or after modification of
setup data.
Backups can be made on any storage media, using Windows Explorer to drag the relevant directory
onto the corresponding drive. All data will be automatically copied.
For cleaning of the instrument (e.g. deck, tips, racks), use a 70% ethanol solution. Use lint-free
tissues only.
For cleaning of system liquid related parts (e.g. container, reservoir, filter, wash station, waste
tubing), use 70% ethanol solution or 0.05% ProClin 950 solution. Use lint-free tissues only.
Caution
Never use bleach to prime, flush, or clean the system tubing.
8.2 Daily Maintenance

Daily maintenance is performed by the operator daily and takes approximately 15 minutes.
Note
Whenever maintenance actions are performed, the maintenance record should be
completed.
Caution
Take appropriate precautions if hazardous liquids are used.
Rinse with appropriate decontaminant and flush completely to minimize contamination
before removing.
Wear protective clothing, gloves, and glasses.
8.2.1 Start Up Maintenance

1. Replacing the system liquid.
a. Empty the system liquid container(s) and the system liquid reservoir (QUANTA-Lyser 4000
only - see “Draining the QUANTA-Lyser 4000 System Liquid Reservoir” section).
b. Refill with fresh system liquid (0.4% Contrad 70 in DI water) (see “System Liquid
Container” section).
2. Flush the system liquid.

a. Flushing the system is crucial to prevent growth of microorganisms or build-up of crystals
or precipitates from reagents, which might affect assay precision and accuracy. It is also
essential to flush the system liquid at the end of each shift. A flush must be executed at
the beginning and end of each day.
b. Perform a flush by going to the Maintenance Operation screen and clicking the Flush
button.
3. Perform tips decontamination.
a. In the Maintenance Operation screen, click the Decontaminate button.
b. Follow the on-screen instructions, placing 70% Ethanol or Isopropyl alcohol in the diluent
position indicated on the screen and clicking the Start button.
4. Flush the wash buffer lines.
a. In the Maintenance Operation screen, click the Prime button.
b. Follow the on-screen instructions, placing all wash lines in their appropriate wash buffers or
DI water if that wash buffer bottle will not be in use.
5. Alternatively, Steps 2-4 can be done in one step by clicking the Daily button and following the
on-screen instructions. The flush, decontaminate, and prime will be done one after another
without additional intervention.
6. Clean the end of the tip.
a. Gently wipe the end of each tip with a 70% ethanol solution impregnated lint-free tissue to
remove any accumulated serum or reagent.
b. Replace tip if damage is seen for probes 3 or 4. If damage is seen on probe 1 or 2,
schedule an engineer to replace the tip.
8.2.2 Shut Down Maintenance:

1. Flush the system liquid.
a. Flushing the system is crucial to prevent growth of microorganisms or build-up of crystals
or precipitates from reagents, which might affect assay precision and accuracy. It is also
essential to flush the system liquid at the end of each shift. A flush must be executed at
the beginning and end of each day.
b. Perform a flush by going to the Maintenance Operation screen and clicking the Flush
button.
2. Perform tips decontamination.
a. In the Maintenance Operation screen, click the Decontaminate button.
b. Follow the on-screen instructions, placing 70% Ethanol or Isopropyl alcohol in the diluent
position indicated on the screen and clicking the Start button.
3. Flush the wash buffer lines
a. In the Maintenance Operation screen, click the Prime button.
b. Follow the on-screen instructions, placing all wash lines in DI water.
4. Alternatively, Steps 1-3 can be done in one step by clicking the Daily button and follow the on-

screen instructions. The flush, decontaminate, and prime will be done one after another
without additional intervention.
5. Clean the end of the tip.
a. Gently wipe the end of the tip with a 70% ethanol solution impregnated lint-free tissue to
remove any accumulated serum or reagent.
b. Replace tip if damage is seen for probes 3 or 4. If damage is seen on probe 1 or 2,
schedule an engineer to replace the tip.
6. Clean the wash station.
a. Clean wash station by pouring 70% Ethanol solution over the wash cups and leave in
contact for 3 – 5 minutes. Rinse thoroughly with distilled or de-ionized water.
7. Clean the barcode reader (BCR) window(s).
a. Clean the BCR window(s) with a lint-free tissue.
Caution
Never use alcohol or alcohol solutions to clean BCR windows.
8. Empty the waste container.

a. Empty the waste container (see “Changing the Waste Container” section).
9. Clean the touch screen monitor.
a. If required, clean touchscreen with a lint-free tissue.
Caution
Never use alcohol or alcohol solutions to clean touchscreen.
8.3 Weekly Maintenance

Weekly maintenance is performed by the operator at the end of the week and takes approximately
30 minutes.
Note
Whenever maintenance actions are performed, the maintenance record should be
completed.
1. As needed, Back up instrument data.

a. Back up “C:\QUANTA-Lyser\Nelson” folder and “C:\QUANTA-Lyser\quantalyser.h2”
database file
2. Clean the system liquid channels.
a. Clean the system liquid channels (see “Cleaning the System Liquid Channels” section).
3. Clean the liquid supply components applicable to particular model.
a. Clean the system liquid container(s) and reservoir (as applicable) (see “Liquid Supply
Cleaning” section).
b. Clean the system liquid filter(s) and the buffer filter(s) (see “Liquid Supply Cleaning”
section).

4. Clean the wash buffer bottles and lines.
a. Prepare a solution of 0.05% ProClin 950 in DI water (i.e. 500µl ProClin into 1L of DI water).
b. Place 0.05% ProClin 950 in the wash buffer bottles (see “Buffer Bottle 2L” section).
c. Perform the Prime Washer action in the Maintenance Operation screen three times to flush
all buffer lines thoroughly.
d. Thoroughly rinse wash bottles with 0.05% ProClin 950, followed by DI water.
e. Perform the Prime Washer action in the Maintenance Operation screen with DI water six
times each to ensure all Proclin has been removed from the system.
5. Rinse the waste container.
a. Rinse the waste container.
6. Clean the deck, modules, and accessories.
a. Remove all racks.
b. Clean deck, exterior of modules, and accessories (such as slide trays) with a damp cloth,
then with 70% ethanol solution using a lint-free tissue.
c. Clean Resa-trax and all racks with a lint free tissue moistened with a soft soap solution.
Always remove soap solution with a lint free tissue moistened with de-ionized water.
8.4 Preventative Maintenance 1

Preventative maintenance 1 (PM1) is performed by the operator every 6 months. This procedure
requires 10 minutes.
Required Material:
• System liquid filters and buffer filters. See below for quantities of each filter on different
models. Note: Some filters come individually, others in packs of 4, 10, etc. Please note the
packaging size when placing orders.
QUANTA-
QUANTA- QUANTA-Lyser
Filter Type Photo Lyser 3000
Lyser 4000 3000 ELISA/IFA
IFA
Metal System Liquid

Filters
4 N/A N/A
066QL0031
(sold individually)
Metal Buffer/System
Liquid Filters
QL4000 – 066QL0338
4 8 8
(sold individually)
QL3000 – 066QL0429
(pack of 4)

QUANTA-
QUANTA- QUANTA-Lyser
Filter Type Photo Lyser 3000
Lyser 4000 3000 ELISA/IFA
IFA
Plastic Buffer Filters

N/A 4 N/A
066QL0430
(pack of 10)
8.4.1 PM1 Procedure

1. Replace all system liquid and buffer filters.
a. Replace the system liquid filters (see “Liquid Filters” section).
b. Replace the buffer filters (see “Liquid Filters” section)
Note
Filter types vary slightly from model to model. Additionally, for the QUANTA-Lyser 3000
ELISA/IFA models, buffer bottles C and D have both plastic buffer filters used for ELISA
wash lines that connect to the ELISA plate washer, as well as metal buffer/system liquid
filters that are used for the system liquid lines as well as the 4 buffer lines that connect to
the IFA tip washer.
Preventative Maintenance 2
Preventative maintenance 2 (PM2) is performed by a certified engineer annually.
8.5 System Liquid and Buffer Handling

8.5.1 Liquid Station
The liquid station contains system liquid containers and buffer bottles. Location varies depending on
instrument model. For QUANTA-Lyser 4000, the system liquid and buffer bottles are contained
within the liquid station drawer, while the QUANTA-Lyser 3000 model has a liquid station that is
placed on the counter to the right of the instrument.
Caution
Do not put a heavy load on the liquid station drawer (present on QUANTA-Lyser 4000
model).
The level of the liquid container is monitored individually (using a load cell weight measuring for
QUANTA-Lyser 4000, using capacitve level sensing for QUANTA-Lyser 3000). To prevent particles
from entering the pump, a filter must be installed on each system liquid and buffer tube.

8.5.1.1 QUANTA-Lyser 4000 Liquid Station Drawer
System Liquid Container (9L)
Tubing Coupling
Buffer Bottle (2L)
System Liquid Filter

Bottle Tray
System Liquid Reservoir
Figure 8-2: QUANTA-Lyser 4000 System Liquid Drawer
Tubing Coupling
Bottle Tray (Position C)
Bottle Tray (Position D)

Bottle Tray (Position F)
Bottle Tray (Position E)
Reservoir Cover Plate
SL Filter Assemblies
SL Reservoir (Position B)
SL Reservoir (Position A)
Figure 8-3: QUANTA-Lyser 4000 System Liquid Drawer Positions

8.5.1.2 QUANTA-Lyser 3000 Liquid Station
System Liquid Container (6L)
Buffer Bottle (2L)
Level Sensor Connector

Level Sensor Indicators
Bottle Tray
Figure 8-4: QUANTA-Lyser 3000 Liquid Station 501
Bottle Position D
Bottle Position F
Bottle Position E
Bottle Position C
Liquid Sensor (5)

SL Container Position A
Figure 8-5: QUANTA-Lyser 3000 Liquid Station 501 Positions
8.5.2 System Liquid Container

8.5.2.1 QUANTA-Lyser 4000 System Liquid Container Installation
The liquid station provides two locations (position A and B) to place two liquid system containers,
each with a capacity of 9 liters.

Bottom Handle
Bottle Cap
System Liquid Container
Top Handle
Figure 8-6: QUANTA-Lyser 4000 System Liquid Container
1. Open the front door and pull out the liquid station drawer. Lift out the system liquid container.
2. Remove the bottle cap and fill the container with the required system liquid.
Note
To prevent the contamination of the system liquid filter, 0.4% Contrad 70 is used. Never
use bleach.
Caution
Observe the maximum filling quantity.
Install the container in the reservoir immediately after filling.
A full container is heavy. Be aware that there is a risk of back injury when carrying a full
container if proper lifting techniques are not used.
3. Tighten the bottle cap well and check the function of the non-return valve by pressing and
releasing the spring-loaded mechanism.
Non-Return Valve
Bottle Cap
Figure 8-7: QUANTA-Lyser 4000 System Liquid Container Cap

Caution
For QUANTA-Lyser 4000, before inserting the liquid container, ensure that the reservoirs
are emptied (see “QUANTA-Lyser 4000 System Liquid Reservoir” section) to avoid any
overflow of the system liquid.
4. Insert the container upside down on the reservoir position A or B. Push the liquid station
drawer back and close the front door.
5. Flush several times.
8.5.2.2 QUANTA-Lyser 3000 System Liquid Container Installation

The Liquid Station 501 provides space (at position A) to place a liquid system container with a
capacity of 6 liters.
Tubing Gland
Container Cap
Handle
Figure 8-8: QUANTA-Lyser 3000 System Liquid Container
1. Hold the tubing gland while turning the container cap counter-clockwise.
2. Place the cap with the tubing on a lint-free tissue to avoid dripping.
3. Fill the container with the required system liquid.
Note
To prevent the contamination of the system liquid filter, 0.4% Contrad 70 is used. Never
use bleach.
Caution
Observe the maximum filling quantity.
4. Place the container in its designated position (A).

5. Tighten the container cap well by turning the cap clockwise while holding the tubing gland.
6. Flush several times.

8.5.3 QUANTA Lyser 4000 System Liquid Reservoir
On the QUANTA-Lyser 4000 model, the system liquid reservoir consists of two individual reservoirs
(A and B) connected by a connection tube to proveid equal liquid levels.
8.5.3.1 Draining the QUANTA-Lyser 4000 System Liquid Reservoir

1. Open the front door and pull out the liquid station drawer. Remove the two system liquid
containers.
2. Disconnect all system liquid filter assemblies. Place filter assemblies on a lint free tissue to
avoid any spillage.
3. Remove the reservoir cover plates from the reservoirs.
4. Lift the reservoirs joined together out of the drawer.
Figure 8-9: System Liquid Reservoir Figure 8-10: System Liquid Drawer
5. Drain the residual system liquid into a sink.

6. Insert the reservoirs in the drawer.
7. Mount the reservoir cover plates and insert the system liquid filter assemblies (order is not
important).
8. Insert the container upside down on the reservoir position A or B. Push the liquid station
drawer back and close the front door.
Caution
Mind your fingers while pushing back the drawer. There is a risk of pinching. Push back
the drawer smoothly to avoid spillage.
8.5.4 Liquid Filters

There are different types of liquid filters for buffers and system liquid on the various QUANTA-Lyser
Models.
The QUANTA-Lyser 4000 instrument uses a System Liquid Filter Assembly for the 4 system liquid
lines and stainless steel liquid filter for the 4 buffer lines that reside above bottle tray positions C and
D.
QUANTA-Lyser 3000 uses the stainless steel liquid filters for the 4 system liquid lines as well as the
4 IFA buffer lines and a plastic liquid filter for the 4 ELISA wash buffer lines.

QUANTA-Lyser 3000 model has labels on the tubing that indicate where the tubing should go and
also what type of filter is required.
Tubing Label Stainless Steel Filter Plastic Filter

System Liquid SL X
1C X
Buffer (Pipetting Tip 2C X
for IFA Well Washing) 1D X
2D X
C X
Buffer (For ELISA Plate D X
Washer E X
F X
Caution
Wear gloves while installing or removing the filter to avoid contamination.
Do not mix up filters and tubing when exchanging filters or tubing.
8.5.4.1 System Liquid Filter Assembly Installation, Removal, and Cleaning

The System Liquid Filter is used on QUANTA-Lyser 4000 models for the 4 system liquid lines.
Figure 8-11: System Liquid Filter Assembly
Installation
1. Slide nut over the tubing with the nut threads facing the connection end of the tubing.
2. Ensure the tubing ferrule is the appropriate size, then place it on the tubing, with the tapered
portion of the ferrule facing the tubing nut.
Note
Make sure the tubing end protrudes from the tubing ferrule by approximately 0.5 mm.

Always replace the ferrule when you replace the filter tubing.
3. Insert the tubing in the filter holder and tighten the tubing nut finger-tight. Test the connection
by pulling gently on the tubing while holding the filter holder.
4. Screw system liquid filter holder and tighten the filter finger tight.
Filter
Filter Holder
Tubing Ferrule (Flangeless

Nut 2.5 mm)
Tubing
Tubing Nut (Flangeless Nut

M6)
Figure 8-12: System Liquid Filter Assembly Components
5. Insert the system liquid filter assembly into the clamping ring of the reservoir cover plate until it
snaps in with an audible click.
System Liquid Filter

Assembly
Cover
Filter Holder Clamping Ring

Reservoir Cover Plate
Figure 8-13: System Liquid Filter Holder
Note
Unused filter ports must be sealed with corresponding covers to prevent dust from
contaminating the system liquid.
Removal
1. Hold the system liquid filter assembly by its holder and pull it straight out of the reservoir cover

plate.
Caution
Never try to remove the system liquid filter assembyl by pulling the tubing.
2. Unscrew the system liquid filter from the filter holder.

3. Unscrew the tubing nut from the filter holder.
Cleaning
1. Remove filters.
2. Put filters in a small ultrasonic bath and clean them with 70% ethanol solution or 0.05%
ProClin® 950 solution for 10 minutes. Repeat procedure or replace filters if they are still dirty.
Caution
Do not clean filters with tools, such as cotton stick, etc.
3. Rinse filters carefully several times with distilled or de-ionized water.

4. Install liquid filters.
8.5.4.2 Liquid Filter Installation, Removal, and Cleaning

There are two different types of liquid filters, one being stainless steel that is used on QUANTA-
Lyser 4000 buffer lines and QUANTA-Lyser 3000 system liquid as well as IFA buffer lines, the other
is a plastic filter that is used for QUANTA-Lyser 3000 ELISA buffer lines.
Figure 8-14: Metal Liquid Filter Figure 8-15: Plastic Liquid Filter
Installation
1. Fully attach filter to the tubing.
2. Tighten the container or bottle cap firmly.
Removal
1. Remove the container or bottle cap (For QUANTA-Lyser 4000, first disconnect tubing coupling
from the buffer bottle by pressing the lock).
2. Pull filter from the tubing. Hold the tubing to avoid any force on the tubing gland.
Cleaning
1. Remove the liquid filters.

2. Put filters in a small ultrasonic bath and clean them with 70% ethanol solution or 0.05%
ProClin 950 solution for 10 minutes. Repeat procedure or replace filters if the filters are still
dirty.
Caution
3. Rinse filters carefully several times with distilled or de-ionized water.

4. Install the liquid filters.
8.5.5 Buffer Bottle 2 L
8.5.5.1 QUANTA-Lyser 4000 Buffer Bottle 2 L

The liquid station provides space to place two buffer bottles (position C and D) each with a capacity
of 2 liters.
Tubing Coupling
Bottle Cap
Figure 8-16: QUANTA-Lyser 4000 Buffer Bottle
Installation
1. Open the front door and pull out the liquid station drawer. Remove the system liquid
containers.
2. Disconnect the tubing coupling from the buffer bottle by pressing the lock.
3. Remove the buffer bottle. Remove the bottle cap and fill with the required buffer.
4. Tighten the bottle cap well.
5. Place the buffer bottle in its designated position and connect the tubing coupling.
6. Insert the system liquid containers. Push the liquid system drawer back and close the front
door.

8.5.5.2 QUANTA-Lyser 3000 Buffer Bottle 2 L
The Liquid Station 501 provides space (positions C – F) to place up to four buffer bottles with a
capacity of 2 liters each.
Tubing Gland
Bottle Cap
Figure 8-17: QUANTA-Lyser 3000 Buffer Bottle
Installation
1. Hold the tubing gland while turning the bottle cap counter-clockwise.
2. Place the cap with tubing on a lint-free tissue to avoid dripping.
3. Fill the container with system liquid.
4. Place the buffer bottle in its designated position (C – F).
5. Tighten the bottle cap well by turning the cap clockwise while holding the tubing gland.
8.5.6 Cleaning the System Liquid Channels

1. Turn the instrument off.
2. Fill a beaker with approximately 0.5 liters of 0.05% ProClin 950 solution.
3. Remove system liquid containers.
4. Place the beaker close to system liquid filter assemblies. Pull them out and submerge in the
solution.
5. Turn the instrument on.
6. Start QUANTA-Lyser software.
7. Go to the “Maintenance Operation” screen.
8. Click “Flush”.
9. Repeat flush procedure twice, for a total of three flush steps.
10. Remove beaker and replace with a beaker containing DI water.
11. Submerge system liquid filter assemblies in DI water.
12. Perform “Flush” maintenance item a total of six times.
13. Replace system liquid filter assemblies and system liquid containers.
8.5.7 Liquid Supply Cleaning

Note
It is recommended that the system liquid filters, the filter holder (QUANTA-Lyser 4000
only), and the buffer filters be cleaned weekly. Filters should be exchanged every 6
months.
Never use bleach.
Caution
Wear gloves while installing or removing filters to avoid contamination.
1. Remove system liquid containers and buffer bottles. Rinse thoroughly with distilled or
deionized water before refilling.
2. Remove system liquid filter(s) and, if applicable, filter holder(s) (see “Liquid Filters” section).
3. Remove buffer filter(s) (see “Liquid Filters” section).
4. Soak filters with 0.05% ProClin 950 solution for 10 minutes. Repeat procedure or replace filter
if the filters are still dirty.
Caution
5. Rinse carefully several times with distilled or deionized water.

6. For QUANTA-Lyser 4000, drain system liquid reservoirs (see “Draining the QUANTA-Lyser
4000 System Liquid Reservoir” section) and clean them with 0.05% ProClin 950 solution.
Rinse carefully with water and wipe the outside.
Caution
Do not clean system liquid reservoir in an autoclave or dishwasher.
7. Install system liquid filters and filter holders, if applicable (see “Liquid Filters” section).
8. Install buffer filter(s) (see “Liquid Filters” section).
9. Fill system liquid containers with system fluid (0.4% Contrad 70) and insert them in the
reservoir (if applicable) (see “System Liquid Container” section).
10. Fill buffer bottles with distilled or deionized water.
11. Flush all liquid channels.
8.6 Waste Handling

The waste station houses the waste container with a capacity of 10 liters. On QUANTA-Lyser 4000
only, the liquid level is monitored by a load cell (weight measuring) generating an alert if the
container is full.
Caution

The waste container may contain biohazardous or toxic material.
A full container is heavy. Be aware that there is a risk of back injury when carrying a full
container. In case a full container is dropped accidentally on the balance pan, the load
cell (if equipped) may be impaired.
To avoid an overflow of the container, the load cell must be checked by a certified service
engineer.
Do not walk or stand on the balance pan.
Inlet from Wash Station
Lid
Cover
Handle
Inlets From Pump Unit
Waste Container (10 L)
Rails
Balance Pan
Waste Station Connector
Stand
Figure 8-18: QUANTA-Lyser 4000 Waste Bottle
Inlets From Wash Station

Lid
Inlets From Pump Unit
Rails
Handle
Waste Container (10 L)
Stand
Figure 8-19: QUANTA-Lyser 3000 Waste Bottle
8.6.1 Changing Waste Container

1. Turn instrument off.
2. Place an empty container next to the waste station. Remove the container cover.
3. Lift the container lid and slightly shake it to avoid dripping. Place the lid in the empty container.
4. Firmly seal the full container with the cover and remove the container from the waste station.
Use the handle to carry the container.
Figure 8-20: Waste Container Removal
5. Dispose of waste according to proper protocol.

6. Place the empty container on the waste station. Ensure the lid is properly inserted in the two
rails and the container is properly sealed.
8.7 Opening the Front Shield Manually

A lock bolt is used to avoid an accidental elevation of the front shield. While the instrument is in
operation, the lock bolt is controlled by the application software. When the instrument is turned off,
the lock bolt must be released manually to elevate and lower the front shield. The lock bolt is
located towards the bottom left side of the instrument.
In rare situations, it may be necessary to unlock the front shield manually. Note that it is not
recommended to open the front shield manually while the system is in use. There is also a door
sensor check performed by the software which will pause the run in the event the cover is found to
be open.

Front Shield
Handle
Handle
Lock Hole
Lock Bolt
Figure 8-21: Front Cover Lock
8.7.1 Elevating and Lowering the Front Shield

Elevating the Front Shield
Insert a screwdriver or the like in the hole at the handle and carefully push the bolt back while
elevating the front shield. Pull out screwdriver.
Lowering the Front Shield
Push back the lock bolt with a screwdriver or the like while carefully lowering the front shield. Pull
out the screwdriver to fully close the front shield.
Caution
Do not try to close the front shield without pushing back the lock bolt.
Mind your fingers while lowering the front shield. There is a risk of pinching
Figure 8-22: Unlocking the Front Cover Figure 8-23: Locking the Front Cover
8.7.2 Cleaning the Housing

When necessary, arcylic panels of the housing can be cleaned either with a dry lint-free tissue or a
lint-free tissue moistened with diluted dish detergent. Alternatively, an anti-static acrylic cleaner can
be used (read instructions provided with the cleaner).

Caution
Never use cleaners containing organic solvents, such as ethanol, acetone, etc.
8.8 Exchanging Tips
8.8.1 Pipetting Tip

Z-Rack
Z-Tube
Z-Rack Blocking Screw
Aspiration Tip Blocking

Screw
Pipetting Tip Blocking

Screw
Aspiration Tip
Pipetting Tip
Figure 8-24: Pipette Tips
Caution
Handle tip with care to avoid damage to the outer coating.
The tip is sharp enough ot be a puncture hazard.
Required Tools
• Allen wrench 2 mm
• Try square
• Piece of a rubber liner or Parafilm

8.8.1.1 Dispense Tip Removal
Note
Probes 1 and 2 have an aspirate probe attached to the dispense probe. The aspirate
probe must be removed before the pipetting tip can be removed. That process requires
assistance from a service provider. Please contact your service provide in case probe 1
or 2 needs to be replaced.
Use a piece of rubber liner or Parafilm to get a better grip while removing and installing
the tubing.
1. To avoid damage due to liquid dripping while removing the tip, cover the deck underneath the
tips with absorbent tissues.
2. Turn the instrument off and unplug the power cable.
3. Elevate the front shield.
4. Loosen the z-rack blocking screw.
5. Loosen the tip blocking screw.
6. Hold the z-rack while carefully pushing the liquid level detector housing up by approximately 20
mm.
7. Disconnect the pipetting tip from the tubing.
Figure 8-25: Disconnecting Dispense Tip
Tip
Rotating the tip while pulling down aids in disconnecting the tubing.
8.8.1.2 Dispense Tip Installation

Note
Probes 1 and 2 have an aspirate probe attached to the dispense probe. The aspirate
probe must be installed before the pipetting tip can be installed. That process requires

assistance from a service provider. Please contact your service provider in case probe 1
or 2 needs to be replaced.
Use a piece of rubber liner or Parafilm to get a better grip while removing and installing
the tubing.
1. Ensure that the instrument is switched off and the power cable is unplugged.
2. Ensure the contact of the pipetting tip touches the terminal at the liquid level detector housing
and then connect the tip to the tubing.
3. Move the pipetting tip upwards until the terminal contact fits into the recess on the liquid level
detector terminal.
Terminal
Contact
Liquid Level Detector

Housing
Pipetting Tip
Figure 8-26: Dispense Tip Installation
4. Tighten the tip blocking screw.

5. Put a try square on the deck and check the X-tilt along the left-hand side of the tip. Parallelism
must be within 0.2 mm on a length of 100 mm. If out of range, either try to bend the tip
carefully in position or replace it.
6. Turn the try square by 90° and check Y-tilt along the front side of the tip. Parallelism must be
within 02 mm on a length of 100 mm. If out of range, either try to bend the tip carefully in
position or replace it.

Figure 8-27: Dispense Tip Parallelism Left/Right Figure 8-28: Dispense Tip Parallelism Front/Back
7. Plug in the power cable and turn the instrument on.

8. Flush the appropriate liquid channel.
8.8.2 Aspiration Tip

Caution
Handle the tip with care to avoid damage to the outer coating.
The tip is sharp enough ot be a puncture hazard.
The aspiration tip, located on probes 1 and 2, requires additional assistance to remove or install.
Please contact your service provider for further assistance.
8.9 Handling Instrument Data

The instrument data containing instrument specific data should be backed up at regular intervals
(once a week, according to the maintenance schedule), or as needed.
8.9.1 Backup Procedure

Locate the “C:\QUANTA-Lyser\Nelson” folder and the “quantalyser.h2” file (in C:\QUANTA-Lyser)
and save a copy of both to an external location, either an external hard drive or flash drive. It is
advised to change to a unique name (such as quantalyserDDMMYYYY) in order to be able to store
multiple backups in the same location.

QUANTA-Lyser® Maintenance Record
QUANTA-Lyser Model ___________SN ________ Month_____________ Year_______
Daily Maintenance 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Start Up
Replace System Liquid
Flush System Liquid
Perform Tips Decontamination
Flush Wash Buffer Lines
Wipe Tips With Alcohol
Shut Down
Flush System Liquid
Perform Tips Decontamination
Flush Wash Buffer Lines
Wipe Tips With Alcohol
Clean Tips Wash Station
Clean Barcode Reader Windows
Empty Waste Container
Clean Monitor
Weekly Maintenance
Back Up Instrument Data
Clean System Liquid Channels
Clean Liquid Supply Components
Clean Buffer Bottles and Lines
Rinse Waste Container
Clean Deck and Modules
PM1 – 6-Month Maintenance
Filters Replacement
PM2 – Annual Maintenance
Professional PM
Initials

Troubleshooting CH. 9
CHAPTER 9: Troubleshooting
The following section describes some potential errors and gives some assistance locating the cause
and possible remedy. If the recommended action does not solve the issue, please contact your
service provider for further assistance.
Issue Description Possible Cause Recommended Action
Problems Related to Liquid Handling

Drop at the end of the tip • Leaky tubing • Flush system liquid channels to remove
• Bubbles in tubing bubbles
• Tip collision • If a tip has crashed, is bent, or has a
damaged coating, exchange the tip
• Clean tip with a 70% ethanol solution
moistened lint-free tissue
Liquid detection problems • Bubbles in tubing • Flush system liquid channels
• Tubing not fully connected • Check connection between tip and terminal
Missing volume in some • Foam/bubbles in sample or • Remove foam manually
dispensed wells reagent containers
Poor pipetting precision • Foam/bubbles in sample or • Remove foam manually
reagent containers • Flush system liquid channels
• Bubbles in tubing • Clean and/or replace system liquid filters
• Clogged/dirty system liquid
filter
Problems Related to Liquid Handling
BCR tool fails to read • Barcodes not positioned • Adjust label so that barcode is positioned
correctly correctly
• Barcodes not clean or • Clean labels or replace them with clean
clear ones
• Barcodes too glossy • Try reprinting barcodes with a higher print
quality
• Replace glossy barcodes with matte ones

9900 Old Grove Road
San Diego, CA 92131
United States of America
Technical Service: 877-829-4745
Technical Service (Outside of US): +1-858-805-7950
support@inovadx.com
www.inovadx.com
Australian Sponsor:
Werfen Australia Pty Ltd

59-61 Dickson Avenue
Artamon NSW 2064 Australia
Tel. +61 2 9098 0200 / 1300 369 132
http://au.werfen.com/
Authorized Representative in the EU:

Medical Technology Promedt Consulting GmbH
Altenhofstrasse 80
66386 St. Ingbert, Germany
Tel.: +49-6894-581020
Fax: +49-6894-581021
www.mt-procons.com
QL3000/4000OPS Rev.4
Effective Date: 11/15/2019
ECO#: 511721

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Page 2 QUANTA-Lyser Operators Manual Rev.4

Page 3 QUANTA-Lyser Operators Manual Rev.4

Page 4 QUANTA-Lyser Operators Manual Rev.4

Page 6 QUANTA-Lyser Operators Manual Rev.4

1.2 Policy Statement

1.3 Symbols and Conventions

Consult the manual for further information, proceed with caution

Consult the manual for further information, proceed with caution

High voltage, possibility of electric shock, proceed with caution

Risks associated with a laser beam

Risks associated with biohazards

Page 7 QUANTA-Lyser Operators Manual Rev.4

Risks associated with chemicals or biological materials

Risks associated with flammable materials

Risks associated with puncture hazard

Advice associated with the use of lubricants

Risks associated with heavy load

Do not walk or stand here

Must be performed by certified engineers only

Consult the manual, proceed with caution (label on instruments only)

Advises special attention

Indicates the manufacturer

Catalogue or Part Number

Page 8 QUANTA-Lyser Operators Manual Rev.4

1.4 Intended Use

Page 9 QUANTA-Lyser Operators Manual Rev.4

Page 10 QUANTA-Lyser Operators Manual Rev.4

CHAPTER 2: Preinstallation and Installation Procedures

2.2 Requirements for Personnel

2.3.2 Mains Supply

Page 12 QUANTA-Lyser Operators Manual Rev.4

2.5 Customization and Training

2.6 Assay Menu

2.7 Labeling and Orientation Precaution

2.7.1 120 mL Becker Vial Labeling

Page 13 QUANTA-Lyser Operators Manual Rev.4

2.7.2 Orientation of Bottles in Racks

2.8 System Requirements

2.8.1 Space Requirements

2.8.2 Power Requirements

Page 14 QUANTA-Lyser Operators Manual Rev.4

Page 15 QUANTA-Lyser Operators Manual Rev.4

Page 16 QUANTA-Lyser Operators Manual Rev.4

CHAPTER 3: Principles of Operation

3.1 System Capabilities

3.1.1 Instrument Capabilities

3.1.2 Software and Applications

3.2.1 Hardware Devices

3.2.1.1 Pipetting Arm and Tips

Figure 3-1: Pipetting Tips

Liquid Detection (LD)

Page 18 QUANTA-Lyser Operators Manual Rev.4

Pumps and Valves

Figure 3-2: Pumps and Valves

Page 19 QUANTA-Lyser Operators Manual Rev.4

Figure 3-4: QUANTA-Lyser 3000 Hydraulic Diagram (Model Dependent)

3.2.1.2 Pipette Tips Wash Station and Waste Station

Figure 3-5: Pipette Tips Wash/Waste Station