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    Microscope Information Sheet

    Uploaded by

    Yaj Cruzada
    Eueidod

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Microscope Information Sheet

    Uploaded by

    Yaj Cruzada
    54 views2 pages
    Eueidod

    Original Title

    Microscope-Information-Sheet.doc

    Copyright

    © © All Rights Reserved

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    DOCX, PDF, TXT or read online from Scribd

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    Eueidod

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    54 views2 pages

    Microscope Information Sheet

    Uploaded by

    Yaj Cruzada
    Eueidod

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 2

    Microscope Information Sheet

    Name: Class: Date:

    A. Microscopes are useful tools for observing very small objects such as cells. There are many
    different types, but the type that we will use
    most often in this class is called a compound
    microscope.

    Parts of the microscope


    a) Eyepiece: magnifies the sample 10x
    b) Stage: flat area where slide is placed
    c) Diaphragm/ Light control disc: varies the
    amount of light from the lamp, adjust to get more
    contrast
    d) Course adjustment: focuses the sample at low
    power objectives by moving the stage a lot
    e) Fine adjustment: focuses the sample at high
    power objective by moving the stage a little
    f) Objective Lenses: the most expensive parts!
    1. High power: magnifies sample by 40x or 44x
    2. Medium power: magnifies sample by 10x
    3. Low power (scanning): magnifies sample by 4x

    B. Care of the Microscope


    1. Carry the microscope with two hands, one hand under the base and the other on the arm.
    2. Keep the microscope away from the edge of your table. When it is necessary to move your microscope,
    pick it up and gently place it where you want it. Do not push or tilt it.
    3. Always clean lenses with only lens paper and lens cleaner. The lenses are very expensive.
    4. When putting the microscope away, return it to the low power setting and wind the electric cord around the
    base of the microscope.

    C. Setting up the Microscope


    1. Always begin looking at a slide under low power.
    2. Make sure slides are clean and dry before use. Carefully place the slide on the stage. If you do not see an
    image, check to make sure the lens is in place and that the light source is on and the outlet is powered.
    3. Focus with the Coarse Focus knob (large one).
    4. Adjust the light control disc or diaphragm to get the clearest image possible. Brighter is not always better!
    5. Make sure the lenses are dry and free of fingerprints and debris. Do not touch the lens.

    D. Using High Power


    Once the slide is focused under low power: ,
    1. Centered the part of the slide that you want to see BEFORE you change the objective lens. The field of
    view (area that you can see) decreases with increasing magnification, so it is easy to lose track of the
    part of the slide you are trying to see
    2. Carefully change the objective lens while watching the lenses (rather than looking through the
    eyepiece). Make sure the lens has enough room to clear the slide.
    3. Adjust the Fine Focus knob only when at high power. Do not use the coarse focus knob.
    4. Readjust the light source. If you cannot find the object, then go back to low power again.
    5. If you are following all of the focusing steps and you still cannot focus the image, go back to low power
    and place a clean blank slide under the slide you are trying to focus. This will raise the slide and make
    it easier to focus.

    E. Image Magnification and Direction


    1. Determine the magnification of the image by multiplying the power of the objective lens by the power of the
    eye-piece. (E.g. eyepiece 10x and objective 10x = 100x magnification)
    2. Note the direction the image appears to move when you use your thumbs and forefingers to move the slide
    away from you, to the right. The object will move in the direction opposite of your movements.
    3. Because most of the objects examined with a microscope are quite small, biologists generally use a unit a
    measure known as a micrometer (or micron) which is equal to 1/1000 of a millimeter. The abbreviation for
    micrometer is um.

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