Thermo Scientific Revco DXF - 40C Upright Laboratory Freezer: Operating Manual 7008813 Rev. 1

Thermo Scientific
Revco® DxF -40C Upright Laboratory Freezer
Operating Manual 7008813 Rev. 1
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www.thermoscientific.com/labwarranty
Preface
Important installer and user information:

A redundant temperature sensing device has been included in this ULT
freezer. This device is a type “T” thermocouple. For convenient access,
the thermocouple (Figure 1-3) terminates in an interconnect jack (Figure
1-5) behind the base front cover. (May be located differently in chests.
See Section 1.) It is strongly recommended that this thermocouple be
attached to a redundant 24 hour 7 day monitoring system with alarm
capabilities. Connecting the sensor to a monitoring and alarm system
separate from the freezer provides the utmost in product safety, should
the integral system fail. s
Models covered by this manual Packing List
Catalog Capacity - Part Number Description Quantity

Model Voltage
Number Cubic Feet
DxF24040A 8813 13 120/60 34040 Key Ring 1
DxF24040V 8814 13 230/50 122005 Key 2
DxF24040D 8816 13 230/60 380520 Neoprene Cap 2
DxF32040A 8817 17 120/60 510016 1/4-20 x 5-1/2” Bolt 2
DxF32040V 8819 17 230/50 195763 Retaining Clip 1
DxF32040D 8820 17 230/60 370563 Remote Alarm Connector 1

DxF40040A 8821 23 120/60
DxF40040V 8822 23 230/50
DxF40040D 8823 23 230/60
MANUAL NUMBER 7008813
1 FR-2202 8/2/11 Updated refrigeration schematics w/ new captube length ccs

0 FR-2202 7/14/11 Original - Release 6 ccs
REV ECR/ECN DATE DESCRIPTION By
Thermo Scientific -40C Lab Freezer i

Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2011 Thermo Fisher Scientific. All rights reserved.
ii -40C Lab Freezer Thermo Scientific

Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Extreme temperature hazards, hot or cold. Use special handling equipment or wear special, protective
clothing.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and information
on Thermo products will be available at www.thermofisher.com.
4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4 Always dissipate extreme cold or heat and wear protective clothing.
4 Always follow good hygiene practices.
4 Each individual is responsible for his or her own safety.
Thermo Scientific -40C Lab Freezer iii

Preface
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Regardless of your needs, our professional telephone technicians are available to assist you
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telephone or fax. If you wish to write, our mailing address is:
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Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.
iv -40C Lab Freezer Thermo Scientific

Table of Contents
Section 1 Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-3
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Freezer Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Choose the Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Wall Bumper Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Attach the Line Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Freezer Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Set Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Set High Temperature Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Set Low Temperature Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Run Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Section 2 Calibrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrate the Control Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Section 3 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Probe Failure Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Section 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Clean the Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean the Condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrost the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Clean the Door Gasket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Clean the Vacuum Relief Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Replace the Battery(s) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Prepare the Unit for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Thermo Scientific -40C Lab Freezer v

Table of Contents
Section 5 Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Vent Stack, Solenoid and Injection Assembly . . . . . . . . . . . . . . . . .5-2
Temperature Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Connect the Probe/Solenoid Harness . . . . . . . . . . . . . . . . . . . . . . .5-4
BUS Operation and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Configure the Optional Back-Up System . . . . . . . . . . . . . . . . . . . .5-5
Set the Optional Back-Up Setpoint . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Test BUS Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Clean Vent Stack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Disconnect the Fitting Assembly & Transfer Hose . . . . . . . . . . . . .5-7
Chart Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Install the Chart Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Change the Recorder Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Calibrate the Chart Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Datalogger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Five Inner Door Option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Section 6 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Section 7 Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Section 8 Refrigeration Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Section 9 Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Section 10 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Section 11 Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1
vi -40C Lab Freezer Thermo Scientific

Section 1 Installation and Start-Up
Vacuum relief
cover plate
Control panel
Optional back-up
system controls
Temperature
recorder (optional)
Figure 1-1. Front View
Figure 1-1
• Control Panel - keypad, displays and indicators.
• BUS (Optional Back Up System) panel.
• Optional temperature recorder - 7 day, one pen or Datalogger.
• Vacuum relief port cover plate.
Thermo Scientific -40C Lab Freezer 1-1

Section 1
Installation and Start-Up
Wall Bumper
(pre-tapped holes)
Optional Back-up
System Connections
Power Switch
(mains
Remote Alarm disconnect)
Contacts Power Inlet
Figure 1-2. Rear View
Figure 1-2
• Remote alarm contacts.
• Power Inlet for power cord connection.
• Optional BUS connections for probe and solenoid.
• Power Switch (mains disconnect).
Shelf
Bracket
Optional Miscellaneous Accessories Probe

Optional - Recorder Probe/Datalogger Probe
Sealant Control RTD/Redundant Type T
(Caution: This is a critical seal. Thermocouple Probe
Seal must be maintained.)
Figure 1-3. Chamber Probe
Vacuum
Figures 1-3 and 1-4 Relief Port
• Vacuum relief port:
pressure equaliza-
tion port.
• Probe cover: houses
control, optional
recorder, datalogger
or 1535 alarm
probes.
Chamber
Probe Cover
Figure 1-4. Vacuum Relief Port & Probe Cover Locations
1-2 -40C Lab Freezer Thermo Scientific

Section 1
Battery power
switch
To
Thermocouple remove filter
interconnect jack
Battery mounting O
bracket
Freezer battery
and optional BUS battery
Figure 1-5. Battery(s) Location and Switch
Figure 1-5
• Battery mounting bracket(s)
• Battery power switch (freezer and BUS)
• Freezer battery
• Optional BUS battery
• Freezer filter location
Alarm Silence Alarm

Control Panel Keys, Mode Key Indicator Key Panel
Displays & Indicators
Temperature Display
Up & Down Enter

Arrows Key
Figure 1-6. Control Panel

Section 1
Control Panel • Temperature Display - Displays temperature in degrees Celsius.

(continued) • Mode Select Switch - Used to select Run, Set Temperature, Set High
Alarm, Set Low Alarm, Calibrate, Backup.
• Alarm Indicator - Light pulses on/off during an alarm condition of the

cabinet.
• Silence - Silences the audible alarm. See Section 4 for alarm ringback
times.
• Alarm Panel - indicates the current alarm condition.
• Up and Down Arrows - Increase or decrease values, toggle between

choices.
• Enter - Stores the changed value into memory.
Keypad Operation The Revco DxF freezer has five basic modes to allow freezer setup and
operation. Press the Mode key to scroll through the mode selections.
Figure 1-7. Basic Modes
Freezer Installation Caution If tipped more than 45°, allow the unit to sit upright for 24 hours
before start up. s
To remove the freezer from the pallet, use the 7/16" wrench to remove all
bolts securing the shipping bracket to the pallet. Remove the shipping
bracket.
Remove the ramp boards from the pallet and place the slotted ends over
the ramp brackets on the pallet. The support blocks on the ramps will be
facing down. Before moving the freezer, make sure the casters are unlocked
and moving freely. Align the caster with the ramp boards. Use adequate
personnel to roll the freezer off the pallet.

Section 1
Freezer Installation The freezer can be easily pushed to the desired approved location. If
necessary, the doors and lower front panel may be opened to move the unit
(continued) through tight openings. When the freezer is in position, set the front caster
brakes.
Caution The freezer must not be moved with product load inside. s
Caution For proper ventilation and airflow, a minimum clearance of 5” at

the rear and top and a clearance of 8” on the side of the freezer is required.
Allow adequate space in the front of the freezer for door opening. s
Choose the Location Locate the freezer on a firm, level surface in an area with an ambient
temperature between 18°C and 32°C. Provide ample room to reach the
mains disconnect switch (power switch) located on the rear of the freezer.
Wall Bumper Installation The parts bag, located inside the cabinet, contains the following parts.
Qty Stock # Description Purpose
2 510016 1/4-20 x 5-1/2” Bolt Wall Bumper
2 380520 Neoprene Cap Cap Protector
Install the bolts into the pre-tapped holes on the back of the compressor
section. Install a neoprene cap on each bolt. Refer to Figure 1-2 for the
locations of the pre-tapped holes.
Shelf Installation Install the shelf clips into the shelf pilasters (front and back) at the desired
shelf level. Install the shelves in the cabinet onto the clips.
Note Maximum shelf load is 100 lbs (45.4 kg) per shelf. s

Section 1
Remote Alarm Contacts See Figure 1-2 for the location of the remote alarm contacts. The remote
alarm connector is located in the parts bag provided with the manual. It
must be installed if connecting the freezer to an alarm system. After
installing the wiring from the alarm system to the connector, install the
connector to the freezer microboard and secure with the two screws
provided. The remote alarm provides a NO (normally open) output, a NC
(normally closed) output and COM (common). The contacts will trip on a
power outage, and high or low temperature alarm. They will also trip on
control probe or microboard failure. Figure 1-8 shows the remote contacts
in alarm state.
IMPORTANT USER INFORMATION

Caution! Stored product should be protected
by a redundant 24 hour/day monitoring
system with alarm capability. An interconnect
jack and thermocouple are installed
for centralized monitoring, should on-board
system fail.
Figure 1-8. Remote Alarms Contacts
Attach the Line Cord Insert the power line cord into the power inlet module. Place the retaining
bracket (P/N 195763) over the connector. Tighten retaining screws to
secure.
Figure 1-9. Line Cord

Section 1
Connect Unit to Electrical Note See the serial tag on the side of the unit for electrical specifications or
Power refer to the electrical schematics in this manual. s
The freezer should be operated on a dedicated grounded service. Check the

voltage rating on the serial tag of the unit and compare it with the outlet
voltage. Then, with the power switch turned off, plug the line cord into
the wall outlet.
First, turn on the freezer power switch. Then open the lower front door by
grasping the bottom left corner. Locate the battery switch (Figure 1-5) and
turn it to Standby mode ( ). During initial freezer start-up, the system
battery may require charging and the Low Battery indicator may
illuminate.
Caution Ensure the battery switch is turned to Standby mode ( ). The

rechargeable batteries require 36 hours to charge at initial start-up. A “Low
Battery” alarm may occur until the batteries are fully charged. Should a
power failure occur during the initial start-up period, the electronics will
have limited operation. s
Freezer Start-Up With the freezer properly installed and connected to power, system
setpoints can be entered. The following setpoints can be entered in
Settings mode: Control temperature, high temperature alarm setpoint, low
temperature alarm setpoint, and (optional) BUS setpoint. Default settings
are shown in the table below.
Control Set Point -40°C

High Temperature Alarm -30°C
Low Temperature Alarm -50°C
Optional BUS Set Point -30°C
Note If the setpoint is changed and the low temperature and high
temperature alarms are set 10° from the setpoint, the alarm setpoints will
be adjusted automatically to maintain a distance of at least 10° from
setpoint. s

Section 1
Set Operating Revco DxF -40°C freezers have an operating temperature range of -10°C
Temperature to -40°C. The freezer is shipped from the factory with a temperature
setpoint of -40°C. To change the operating temperature setpoint:
1. Press the Mode key until the Set Temperature indicator lights.
2. Press the up/down arrow key until the desired temperature set point is
displayed.
3. Press Enter to save the setpoint.
4. Press the Mode key until the Run indicator lights for Run mode
If no keys are pressed, the freezer will automatically return to RUN mode
after 5 minutes.
Set High Temperature The high temperature alarm activates an audible/visual warning when the
Alarm freezer chamber temperature has reached or exceeded the high temperature
alarm setpoint.
To set the high temperature alarm setpoint:
1. Press the Mode key until the Set High Alarm indicator lights.
2. Press the up or down arrow key until the desired high temperature
alarm setpoint is displayed.
3. Press Enter to save the setting.
4. Press the Mode key until the Run indicator lights for Run mode.
If no control keys are pressed, the freezer will automatically return to RUN
mode after 5 minutes.
Note The high alarm set point must be set at least 5°C from the control
set point. s
Caution At initial start-up, the high temperature alarm is disabled until the
cabinet reaches setpoint, or 12 hours elapse. s

Section 1
Set Low Temperature The low temperature alarm activates an audible/visual warning when the
Alarm freezer chamber temperature reaches or decreases below the low
temperature alarm setpoint.
To set the low temperature alarm set point:
1. Press the Mode key until the Set Low Alarm indicator lights.
2. Press the up or down arrow key until the desired low temperature
alarm setpoint is displayed.
If no control keys are pressed, the freezer will automatically return to RUN
Note The low alarm set point must be set at least 5°C from the control set
point. s
Run Mode Run mode is the default mode for the freezer. Run mode displays the
cabinet temperature on the temperature display under normal operating
conditions. In addition, Run mode shows the high stage heat exchanger
temperature.
This information is scrolled by pressing the up or down arrow keys. The

display will return to the operating temperature in 10 seconds if no keys
are pressed.

Section 2 Calibrate
After the freezer has stabilized, the control probe may require calibration.
Calibration frequency is dependent on use, ambient conditions and
accuracy required. A good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.
Caution Before making any calibration or adjustments to the unit, it is

imperative that all reference instruments be properly calibrated. s
Calibrate the Control Plug a type T thermocouple reader into the receptacle inside the lower
Probe door (Figure 1-5). Compare the control temperature setpoint to the
temperature of the measuring device.
1. Press the Mode key until the Calibrate indicator lights.
2. Press up/down arrow to match the display to calibrated instrument.
3. Press Enter to store calibration.
4. Press the Mode key to return to Run mode.
Temperature Stabilization Periods

Start-up - Allow 12 hours for the temperature in the cabinet to stabilize
before proceeding.
Already Operating - Allow at least 2 hours after the display reaches set
point for temperature to stabilize before proceeding.
Caution During calibration, the temperature display is disabled. s
If no keys are pressed for approximately five minutes while in Calibration

mode, the system will return to Run mode.

Section 3 Alarms
Revco DxF freezer alarms are displayed on the freezer control panel.
When an alarm is active, the indicator next to the alarm description will
light and there will be an audible alarm. Press the Silence key to disable the
audible alarm for the ringback period. The visual alarm will continue until
the freezer returns to a normal condition. The alarms are momentary
alarms only. When an alarm condition occurs and then returns to normal,
the freezer automatically clears the alarm condition.
Description Delay Ringback Relay
Power Failure 1 min. 15 min. Yes
High Temperature Alarm 1 min. 15 min. Yes
Low Temperature Alarm 1 min. 15 min. Yes
Probe Failure 1 min. 15 min. No
Door Open 1 min. 15 min. No
Wrong Power 0 min. none Yes
Low Battery* 1 min. 12 hours No
Low BUS Battery (optional) 1 min. 15 min. No
Hot Condenser 1 min. none No
MicroBoard Failure 0 min. 15 min. Yes
All alarm delays and ringback times are ±30 seconds.

*The automatic battery test runs immediately on initial start-up, then every 8 hours thereafter.
Probe Failure Alarm The microprocessor in Revco DxF freezers continually scans all probes,
including the control probe, heat exchanger probe and condenser probe, to
ensure that they are operating properly. Should an error be detected, the
"Probe Failure" alarm occurs as described above. If an error is detected
with the control probe, the high and low stage compressors run
continuously. As a result, the cabinet temperature decreases until it reaches
the lowest temperature the refrigeration system can maintain. If an error is
detected with the heat exchanger probe, the freezer cycles properly at its
temperature setpoint using a 5 minute step-start between the high and low
stage compressors. If an error is detected with the condenser probe, there
is no impact on the performance of the freezer; however, the hot condenser
alarm may also occur. Contact the Technical Services department or your
local distributor.

Section 3
Alarms
Error Notes
Name: Improper model selected.

Er00 Description: Indicates that DIP SW3 has not selected a proper model or can’t be accessed properly.
Response: Display shows “Er00” and will not start-up until a proper model is selected. Contact Technical Services.
ErA1 This error condition will prevent peripherals (fans, compressors, etc.) from powering up with the incorrect voltage.
Name: Voltage/Frequency failure
Description: Indicates the measured RMS line voltage did not agree with the logic level sensed by the micros provided by the
ErC1 installed high voltage PCB; or the measured RMS voltage is not within a tolerable range (<180VAC < 270 for 230VAC unit / <
85VAC < 160 for 115VAC unit); or the frequency measured over 10 cycles was not within a tolerable range (55 Hz < Freq < 70 Hz
for 60 Hz units / 40 Hz < Freq < 55 Hz for 50 Hz units)
Erd1
Response: This condition is checked at power on reset and if it is active, the unit will NOT power up. The unit will indefinitely
display “Er_1” in the display and continue to monitor the frequency and voltage. Furthermore, the audible alarm will sound. Other
startup error messages may be displayed prior to this message; however, the system will stop the startup sequence for this condi-
ErE1
tion.
ErA1 .. No pulses (zero crossings) detected to determine frequency (50 / 60 Hz)
ErF1 ErC1 .. Frequency detected is below 50 Hz
Erd1 .. Frequency detected is above 60 Hz (Possible noise spikes on supply voltage)
ErE1 .. Unit is 230V and the voltage detected is below the low limit (180VRMS)
Erg1 ErF1 .. Unit is 230V and the voltage detected is above the high limit (260VRMS)
Erg1 .. Unit is 115V and the voltage detected is below the low limit (85VRMS)
ErH1 ErH1 .. Unit is 115V and the voltage detected is above the high limit (160VRMS)
Name: Control (Cabinet) Sensor Failure

Description: This condition indicates that the control sensor has failed to produce a valid reading for >12 consecutive reads (~60
seconds).
Er02
Response: The unit will stage both compressors on (if necessary) and the unit will attempt to head to bottom out. If the sensor
recovers, the system will begin to operate normally and respond to the temperature feedback. The remote alarm contacts will
become active. ‘Er02’ will be added to the main display queue and the last valid cabinet temperature value will not be displayed.

Section 3
Alarms
Error (cont.) Notes
Name: Display Firmware Integrity Failure

Description: The display firmware has failed to pass its CRC CCITT checksum integrity test.
Er05
Response: The display performs this check at startup and the display board will fail to startup without any error indication if it
does not pass this at power on.
Name: Micro Firmware Integrity Failure

Description: The micro firmware has failed to pass its CRC CCITT checksum integrity test.
Er06
Response: This is checked at power on reset and the “Er06” will be displayed for ~10 seconds at startup if this condition
exists.
Name: Micro Fail - CS5521 SPI Failure / UISR Failure

Description: This condition indicates a micro board failure due to either the SPI bus is unable to communicate with the ADC
device or a UISR event caused the microcontroller to be in an unstable state.
Response: The unit will try to recover from this fault three times by a hardware reset of the micro board. In the event that the
system couldn’t rectify the issue, the following sequence of events will occur:
Er07 1. Remote alarm contacts will become active.
2. Buzzer will annunciate audibly and will have a ringback of 15 minutes.
3. “Seven segment” display will show “Er07”.
4. The compressor will activate.
5. The system will go to bottom out temperatures.
Name: Stuck Button

Er09 Description: This condition indicates that the display board has a stuck button.
Response: The Er09 will show on the display periodically.
Name: Condenser Probe Sensor Failure

Description: This condition indicates that the condenser probe sensor has failed to produce a valid reading for >12 consecutive
Er11
reads (~60 seconds).
Response: The display shows “Er11”.
dErr This is a general display error in which value being displayed can not be represented within characters provided.
Name: Lost Communication

(Four dashes)
Description: Communication between the micro board and the display board has been lost. Under this condition, the visual
---- in display
alarm flashes along with dashes in the temperature display (----). Contact Technical Services.

Section 4 Maintenance
Warning Avoid the excessive use of water around the control area due to
the risk of electrical shock. Damage to the controls may also result. s
Wipe down the freezer exterior using soap and water and a general-use
laboratory disinfectant. Rinse thoroughly with clean water and dry with a
soft cloth.
Clean the Air Filter The procedure for cleaning the air filter (minimum of four times a year)
follows.
1. Open the front lower door by grasping the bottom left corner.
2. Locate the grille on the door. See Figure 1-5. Grasp the middle of the
grille material and gently pull out to remove.
3. Wash the filter material using water and a mild detergent.
4. Dry by pressing between two towels.
5. Install the filter back into the grille and attach the grille.
Depending upon environmental conditions, the filter may need to be

cleaned or replaced more frequently. If the filter becomes torn or
excessively dirty, a replacement can be purchased from Thermo. See the
exploded parts list for the filter part number. A filter kit (set of 5) is also
available.

Section 4
Maintenance
Clean the Condenser The procedure for cleaning the condenser (minimum yearly) follows.
1. Open the front lower door by grasping the bottom left corner. See
Figure 1-5.
2. Using a vacuum cleaner, exercising care to not damage the condenser

fins, clean the condenser.
Depending upon environmental conditions, the condenser may need to be

cleaned more frequently.
Caution Do not use liquids that are corrosive to stainless steel or the
brazing material (copper or nickel). s
Defrost the Chamber The procedure for defrosting the chamber follows.
1. Remove all product and place it in another freezer.
2. Turn the unit off and disconnect it from the power source.
3. Turn off the battery switch (O). See Figure 4-7.
4. Open all of the doors and place towels on the chamber floor.
5. Allow the frost to melt and become loose.
6. Remove the frost with a soft cloth.
7. After defrosting is complete, clean the interior with a non-chloride

detergent. Rinse thoroughly with clean water and dry with a soft cloth.
8. Plug unit in and turn power switch on.
9. Turn the battery power switch to Standby mode ( ).
10. Allow the freezer to operate empty overnight before reloading the
product.

Section 4
Maintenance
Clean the Door The procedure for cleaning the door gasket (minimum monthly) follows.
Gasket Using a soft cloth, remove any frost build-up from the gasket and door(s).
The door gasket may need to be cleaned more frequently if dirt or
excessive frost build-up prevents the door from closing properly.
Clean the Vacuum The exterior door gasket provides an excellent seal that protects product,
provides an energy efficient thermal barrier to keep cold air in and room
Relief Port temperature air out and reduces frost build up on the inner doors.
Because the door gasket seals so well, a vacuum can be created after a door
opening. Warm air enters the cabinet, cools and contracts, creating a
vacuum that pulls the door in tightly against the seal.
To equalize the pressure inside the cabinet after a door opening requires
1.5-3.0 cu.ft. of ambient air to be drawn into the cabinet. The amount of
air required to equalize the pressure varies depending on the cabinet size,
cabinet temperature, duration of door opening, inventory volume and the
temperature/humidity of the ambient air. The unit is designed with a
‘vacuum relief port’ to allow the pressure to be equalized.
The time required to draw 1.5-3.0 cu.ft. of air into the cabinet depends on
two factors,
a) size and number of paths available for the air to enter the cabinet,
b) pressure difference between the internal cabinet and the ambient room.
Cabinets with the vacuum relief port

Vacuum
operating normally, (i.e. vacuum relief port is
Relief Port
not iced over) will require a minimum of 30
seconds up to a maximim of 120 seconds for
the cabinet to equalize. This is also a good
indication that the exterior door is well
sealed.
The vacuum relief port requires routine

maintenance. It will ice over unless
preventive measures are taken. If the
vacuum relief port becomes iced over, the
freezer will take several hours to equalize
pressure.
Figure 4-1. Port Location

Section 4
Maintenance
Clean the Vacuum Caution Do not leave the freezer unattended with the door unlatched. The
vacuum could release, resulting in a door opening and possible product
Relief Port (cont.) loss. s
Observe the inner side of port with each door opening for frost and ice
build-up. Remove any frost with a soft dry cloth. If the tube should
become clogged with ice, it must be cleaned. Make sure during cleaning
that the vacuum relief tube is completely free of ice to prevent rapid ice
formation.
Other factors that can affect the performance of the vacuum relief port
include: high ambient temperature, high humidity conditions and frequent
door openings. Maintenance should be performed weekly, or as needed.
Caution Failure to maintain the vacuum relief port may result in excessive
ice build-up inside the tube, clogging the port, resulting in an inability to
open the door. The vacuum relief port may need to be cleaned more often
with frequent door openings and high humidity environments. s
Replace the Battery(s) 1. To gain access to the battery, open the lower door by grasping the
bottom left corner. The battery is rectangular in shape, located on the
front left corner of the compressor compartment and is secured in
place by a mounting bracket.
2. Directly above the battery(s) is the battery power switch. Turn the
battery power switch to the Off position (O).
3. Disconnect the battery connections.
4. Remove the old battery and install the new battery.
5. Reconnect the battery (red to positive and black to negative).
7. Turn the battery power switch to Standby mode ( ).
8. Close lower panel door.
Caution The % of charge can vary depending on the age, usage and
condition of the battery. For a consistent and dependable charge, replace
the battery every 2 years. Replacement batteries must be rechargeable and
are available from Thermo. Refer to the parts list for stock number and
description of the replacement batteries. Dispose of used batteries in a safe
manner and in accordance with good environmental practices. s

Section 4
Maintenance
Prepare the Unit for Defrost the unit as described previously. This prepares the unit for storage.
Turn off the battery power switch (O). Turn off the freezer power switch.
Storage Disconnect power to the battery(s) and to the freezer.
Caution If the unit has been in service, turn it off and disconnect the
power cord connector before proceeding with any maintenance. s
Battery power
switch
Thermocouple
interconnect jack
Battery mounting O
bracket
Freezer battery
and optional BUS battery
Figure 4-2. Battery Location

4-6
PREVENTIVE MAINTENANCE
Freezers
Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your unit functioning properly. The
-40C Lab Freezer

operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and efficiency, it is recommended that the unit be checked and
calibrated periodically by a qualified service technician.
The following is a condensed list of preventive maintenance requirements. See the specified section of the instruction manual for further details.
We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive Maintenance or Extended Warranties,
please contact us at the number below.
Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.
Tips:
• Fill an upright by starting at the bottom near the probe and add racks to one shelf at a time. Allow freezer to recover to set point between shelves.
• Fill a chest by starting at the left side near the probe. Filling with room temperature racks will result in a long pull-down time.
• Fill unit with frozen product to help overall performance; frozen water jugs, for example.
• Always make certain the vacuum relief port is free of frost and ice, to allow for timely re-entry into the freezer after a door opening.
• 401 Millcreek Road, Box 649 • Marietta, Ohio 45750 USA • 740-373-4763
• USA and Canada 888-213-1790 • Telefax: 740-373-4189 • email: services.controlenv@thermo.com/forma
Thermo Scientific
Thermo Scientific
Preventive Maintenance for )'6#4FSJFTFreezers
Refer to Manual Section Action Monthly Yearly Every
2 Years
-- Verify ambient temperature, <90°F
-- * Adjust door handle for firm latching, as needed
Figure 1-4 for probe location Check and clean probe cover, gaskets, hinges, and vacuum relief port
4.5, 4.6 of ice and snow More frequent cleaning may be
required, depending on use and
environmental conditions
4.2 Check air filter. Clean or replace as needed
1.5.f, 4.7 Check alarm back-up battery ** Replace
-- Check condenser fan motor for unusual motor noise or vibration
2 * Verify and document calibration, at the minimum, annually
4.3 * Clean condenser compartment and wipe off condenser
* Qualified service technicians only
** Dispose of properly, according to all state and federal regulations
To minimize ice build-up inside of freezer:

• Locate the freezer away from drafts or heating/cooling vents
• Keep the number of door openings to a minimum
• Minimize the length of time door is open
• Make sure door latches securely after opening
-40C Lab Freezer

4-7
Section 5 Factory Installed Options
Described below are the factory installed options which are available, or
may be already installed, on your unit.
Back-Up System (BUS) Warning Before installation of BUS components, make sure the power to
the freezer is disconnected, the battery switch is turned off (O) and the
- P/N 1950533, 1950535 freezer has warmed to ambient temperature. s
The built-in BUS (back up system) will keep the freezer chamber
temperature below the critical level in the event of a power or equipment
failure. If power to the freezer fails, or temperature increases to the back up
alarm set point, the BUS injects liquefied gas into the chamber to keep the
chamber temperature within the specified range.
The BUS operates on an internal 12-volt, rechargeable battery which is

kept charged during normal operation by the integral battery charger.
Figure 5-1. Injection Assembly

Section 5
Factory Options
Vent Stack, Solenoid and 1. Install the injection assembly (Figure 5-1) through the 1/2” pre-
Injection Assembly punched hole, directly behind the 2” vent stack hole in the center of
the chamber ceiling.
Note Cover the open end of injection assembly with tape to keep
insulation from entering the nipple. s
2. Slide 3/8” flatwasher over open end of nipple.
3. Insert the covered end of the injection assembly through the exterior
hole.
4. Remove the tape covering from the end of the nipple and install the
1/8” NPT brass tee on the open end of the nipple. Place Permagum
sealant between the brass tee and the interior top.
5. Remove the two Phillips head screws securing the metal bracket on the
vent stack assembly.
6. Install the vent stack (Figure 5-2) through the opening and secure it to
the top of the freezer, using screws.
7. On the cabinet interior, seal around the end of the vent stack with
Permagum.
8. Install the transfer hose connecting one end to the injection assembly,
the other end to the solenoid valve. Install the solenoid valve to the
supply source. The solenoid mounting bracket is not required and may
be discarded.
Caution When selecting a CO2 supply cylinder, it must be equipped with a

siphon tube. s
Figure 5-2. Vent Stack Assembly

Section 5
Factory Options
Temperature Probe 1. Locate the 0.500” pre-punched hole in the upper left hand back corner
of the chamber ceiling. Remove the tie wrap securing the coiled
probe/solenoid harness. Uncoil the probe lead and run the probe tip
(approximately 12”) down through 0.500” porthole (Figure 5-4).
2. As shown in Figure 5-3, thread the small tie wrap through the
openings in the front of the bracket. Secure the probe on the back of
the bracket with the tie wrap.
3. Tap #8-32 the two pre-punched holes located on the interior left wall
of the freezer. Mount the bracket. Figure 5-4 shows the Back-Up
probe mounted on the interior left side wall of the freezer.
195419
Probe Mounting
Bracket
30037
Small Tie
Wrap
290167
Back-Up Probe
Figure 5-3. Probe
Figure 5-3. Bracket

Section 5
Factory Options
Connect the Probe/ 1. Remove the four screws on the freezer back panel and use them to
Solenoid Harness mount the tie wrap anchors as shown in Figure 5-5. Secure the probe
wire with tie wraps.
2. Plug the solenoid/probe connector into the BUS connection and secure
with a screw on the right and left side. The connector is keyed.
3. Loosen the terminal screws on the solenoid. Slide the spade lug
connectors under the screws and tighten to secure.
4. Connect power to the freezer. Turn the freezer On, with battery switch
Off (O).
a. The Solenoid Engaged light on the BUS control panel will

illuminate (no injection occurs). This light stays on until the unit is
below BUS setpoint.
b. The Low Battery indicator may also illuminate.
5. Turn the battery switch to Standby mode ( ) to charge both batteries.
Tie wrap
anchor
Spade lug
connections Probe wire
to solenoid
BUS connection
Power switch
(mains disconnect)
Remote Alarm
Contacts Power Outlet
Figure 5-5. Routing

Section 5
Factory Options
BUS Operation and Warning When activated, this unit injects liquid nitrogen or carbon
Maintenance dioxide. Liquid nitrogen can cause serious freezing (frostbite) if it comes in
contact with unprotected skin or eyes. Nitrogen suppresses oxygen levels
and may cause suffocation if area is not well ventilated. Refer to Appendix
A for the proper handling of liquid LN2. s
Caution Make sure the pressure relief valve on any LN2 tank is adjusted to
30 PSI maximum blow-off. s
Warning Carbon dioxide gas suppresses oxygen levels and may cause
suffocation if area is not well ventilated. Refer to “Handling Liquid CO2”
in Appendix B of this manual. s
Power - indicates the unit has AC power.

Low Battery - battery charge is low. The battery needs replaced or
recharged.
Solenoid Engaged - BUS has opened the solenoid so it can inject gas (CO2
or LN2).
Press-To-Test - Activates the solenoid and injects LN2 or CO2 into the
freezer chamber as long as the button is depressed. The solenoid engaged
indicator should light. If the Low Battery indicator lights during the test,
replace the BUS battery.
Note Solenoid will not engage if door is open. s
Figure 5-6. BUS Control Panel
Configure the Optional The optional BUS can be configured for LN2 or CO2 supply. To select the
Back-Up System supply type:
1. Press the Mode key until the Back-up indicator lights.
2. Press the up or down arrow key. The display will show ‘Co2’ for CO2
selection and ‘Ln2’ for LN2 selection.
If no control keys are pressed, the freezer will automatically return to Run

Section 5
Factory Options
Set the Optional Back-Up The optional back up system is designed to inject CO2 or LN2 into the
Setpoint freezer compartment if the temperature rises above back up system set
point. To set the BUS set point:
1. Press the Mode key until the Set Temperature and Backup indicators
light.
2. Press the up or down arrow key until the desired BUS set point is
displayed.
If no control keys are pressed, the freezer will automatically return to Run
Caution Changing the operating temperature set point can affect the BUS
set point. The BUS set point will self adjust to maintain a temperature of
at least 10°C above the operating temperature set point. s
Caution The BUS set point cannot be set any colder than the high
temperature alarm set point (see Section 1). If the back-up system is
installed with CO2, then -65°C is the coldest BUS set point that can be
used (if the cabinet set point is -75°C or colder). s
Test BUS Operation After the freezer has stabilized and both batteries are fully charged, the
BUS can be tested to verify proper operation.
1. Disconnect AC power to the freezer by turning the power switch off.
2. As the freezer warms up, verify the BUS injects at the desired
temperature. Displayed temperature may vary by a few degrees from
inject temperature due to the differences in probe locations.
Clean Vent Stack Routinely check the vent stack for frost or ice build-up. The type of frost
that forms in the vent stack is generally very soft and may be easily
removed with a bristle brush or soft cloth. If ice build-up has occurred, a
complete defrost may occasionally be required. See Section 4 for freezer
defrost instructions.

Section 5
Factory Options
Disconnect the Fitting To disconnect the freezer back-up from the gas supply:
Assembly & Transfer Hose
1. Close the supply valve.
2. Depress the test button on the BUS control box to remove the gas
from the line.
3. Slowly disconnect the fitting assembly from the supply (in the event
that any gas remains in the line).
Chart Recorder Procedures concerning the use and maintenance of the optional recorder
follow.
3 Program selection and 3

1 2
calibration buttons 1 2
Range Sticker
GOOD
LOW
8
7
1
6
2
5
4
Figure 5-7. Recorder Details
Install the Chart Paper 1. Open the glass door of the recorder and press button #3 3
until the pen begins to move outward.
1 2
2. Unscrew the knob at the center of the chart and remove Figure 5-8.
the paper. Buttons
3. Install the new chart paper, position the paper to the correct time line
and replace the knob.
4. Remove the cap from the felt pen and press button #3.

Section 5
Factory Options
Change the Recorder The chart recorder contains eight temperature ranges and is factory-
Range programmed for the freezer.
1. Press and hold button #3 for one second, then let the pen move off the
chart paper.
2. Press and hold for five seconds either button #1 or button #2.
3. Release the button and the green LED will begin to flash. Count the
number of flashes to determine the present program setting.
4. To change the program setting, Program From To

press the left or right arrows to 1 -40 30°C
increase or decrease the count. 2 0 60°C
5. When the desired program 3 -100 38°C

number is flashing, press button 4 -5 50°C
#3 to bring the pen arm back 5 0 100°C
onto the chart. Recording will 6 -100 200°C
begin in the new program.
7 -115 50°C
8 -10 70°C
Calibrate the Chart Caution The recorder must be in service for 24 hours before performing
Recorder the following calibration procedure. s
1. Place an accurate thermometer in the chamber next to the recorder

probe.
2. Temperature probes for the recorder are located in the left front corner
of the freezer chamber (Figure 1-4).
3. After about three minutes, compare the thermometer reading with the
chart recorder reading.
4. If an adjustment is necessary, press the #1 button to move the pen to

the left or the #2 to move the pen to the right. The button must be
held about five seconds before the pen begins to move. Release the
button when the pen position matches the thermometer.
Note The felt-tip pen on the recorder requires periodic replacement.

Usually the ink will appear to fade before replacement becomes necessary.
Additional pen tips may be purchased from Thermo. s

Section 6 Specifications
Number (Model) DxF24040A (8813) DxF24040V (8814) DxF24040D (8816)
Temperature Range -10°C (-14°F) to -40°C (-40°F)
33.3”W x 77.9”H x 32.9”
Exterior Dimensions
84.6 x 197.9 x 83.6cm
23.0”W x 51.5”H x 19.3”
Interior Dimensions
58.4 x 130.8 x 49.0cm
Capacity 13.0 cu. ft. (368.1 liters)
Refrigeration One 1 HP (2545 BTUH each)
Insulation Non-CFC, foamed-in-place urethane: 5.0” (12.7cm) cabinet; 4.5” (11.4 cm) door
Electrical - 120V, 60 Hz, 10 FLA 230V, 50 Hz, 6.5 FLA 230V, 60 Hz, 6.5 FLA
nominal voltage ±10% Operating Range: 208-230VAC Operating Range: 208-230VAC Operating Range: 208-230VAC
15 amp Dedicated Circuit, 15 amp Dedicated Circuit, 15 amp Dedicated Circuit,

Breaker Requirements
15 Amp Time Delay Breaker 15 Amp Time Delay Breaker 15 Amp Time Delay Breaker
Shipping Weight: Motor 711 lbs. (323 kg)

33.3”W x 77.9”H x 38.9”
Exterior Dimensions
84.6 x 197.9 x 98.8cm
23.0”W x 51.5”H x 25.3”
Interior Dimensions
58.4 x 130.8 x 64.3cm
Capacity 17.3 cu. ft. (489.9 liters)
Electrical - 120V, 60 Hz, 10.0FLA 230V, 50 Hz, 6.5FLA 230V, 60 Hz, 6.5FLA
20 Amp Dedicated Circuit, 15 amp Dedicated Circuit, 15 amp Dedicated Circuit,

Shipping Weight: Motor 830 lbs. (376 kg)

Section 6
Specifications

Exterior Dimensions 40.8”W x 77.9”H x 38.9” (103.6 x 197.9 x 98.8 cm)
Interior Dimensions 30.6”W x 51.5”H x 25.3” (77.7 x 130.8 x 64.3 cm)
Capacity 23.0 cu. ft. (651.3 liters
Electrical - 120V, 60 Hz, 6.5 FLA 230V, 50 Hz, 6.5 FLA 230V, 60 Hz, 6.5 FLA
20 Amp Dedicated Circuit, 15 amp Dedicated Circuit, 15 amp Dedicated Circuit,

Shipping Weight: Motor 940 lbs. (426 kg) 940 lbs. (426 kg) 940 lbs. (426 kg)
Certifications
Declaration of Conformity is available from the factory
Safety Specifications
Indoor Use Only
Altitude - Up to 2,000 meters
Temperature - 5°C to 43°C
Humidity - Maximum RH 80% for temperatures up to 31°C, decreasing linearly to 50% RH at 40°C
Mains Supply Fluctuations - Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage
Installation Category II 1
Pollution Degree 2 2
Class of Equipment I
Intended Use
This product is intended for use as a General Purpose Laboratory Freezer for storing samples or inventory
between -40 and -86C.
This unit is not intended for use in an explosive environment, nor to be used for the storage of flammable
inventory. This unit is not intended for use in a Class II medical application as defined by Title 21 of the
Federal Code of Regulations.
1
Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand
safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II which is the
category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research
laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V
supply.
2
Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that
normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

Section 7
Parts List
Exploded Drawing
Single Door Assy
(R - H - I - -40)
8800-200-1-B Rev. 0
Page 1 of 2

Section 7
Parts List
Exploded Drawing
Single Door Assy
(R - H - I - -40)
8800-200-1-B Rev. 0
Page 2 of 2

Section 7
Parts List
Exploded Drawing
Single Door Assy
(R - H - I - -40)
8813-201-1-D Rev. 0
Page 1 of 2

Section 7
Parts List
Exploded Drawing
Single Door Assy
(R - H - I - -40)
8813-201-1-D Rev. 0
Page 2 of 2

Thermo Scientific
Exploded Drawing
Freezer Base Assy
(F - TS - R - H - I - -40)
8800-203-1-B Rev. 0
-40C Lab Freezer

Page 1 of 2
7-5
Parts List
Section 7
7-6
Parts List
Section 7
-40C Lab Freezer

Exploded Drawing
Freezer Base Assy
(F - TS - R - H - I - -40)
8800-203-1-B Rev. 0
Page 2 of 2
Thermo Scientific
Section 7
Parts List
Exploded Drawing
230V Relay Enclosure
Freezers
902-204-1-B Rev. 0
Page 1 of 2

Section 7
Parts List
Exploded Drawing
Freezers
902-204-1-B Rev. 0
Page 2 of 2

Section 7
Parts List
Exploded Drawing
Freezers
902-204-2-B Rev. 0
Page 1 of 2

Section 7
Parts List
Exploded Drawing
Freezers
902-204-2-B Rev. 0
Page 2 of 2

Section 8
Refrigeration Schematics

Section 8
Refrigeration Schematics

Section 9
Electrical Schematic
120V
-40C Upright Freezers
8800-70-1-D Rev. 0
Page 1 of 3

Section 9
Electrical Schematics
120V
8800-70-1-D Rev. 0
Page 2 of 3

Section 9
120V
8800-70-1-D Rev. 0
Page 3 of 3

Section 9
230V
8801-70-1-D Rev. 0
Page 1 of 3

Section 9
230V
8801-70-1-D Rev. 0
Page 2 of 3

Section 9
230V
8801-70-1-D Rev. 0
Page 3 of 3

Thermo Scientific
THERMO FISHER SCIENTIFIC FREEZER WARRANTY
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection
extends to any subsequent owner during the warranty period.
During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship
will be repaired or replaced at Thermo's expense, labor included. The ULT Freezers include an additional two year warranty
on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The
Technical Services Department must be contacted for warranty determination and direction prior to any work being per-
formed. Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty.
In addition to the standard warranty, the foamed-in-place cabinet design carries a unit production lifetime warranty (foamed-
in-place cabinet, evaporator and foamed-in-place door; parts only). Please contact your sales representative or Thermo for
additional information.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original two year warranty period. The Technical Services Department must
give prior approval for the return of any components or equipment.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation, and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service, and
special applications. Outside the USA, contact your local distributor for warranty information.
ISO
9001
Rev. 0 6/11 REGISTERED
-40C Lab Freezer

10-1
Warranty Information
Section 11
10-2
Section 11
Warranty Information
-40C Lab Freezer

THERMO FISHER SCIENTIFIC FREEZER INTERNATIONAL DEALER WARRANTY
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping time
so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection
extends to any subsequent owner during the warranty period. Dealers who stock our equipment are allowed an additional four
months for delivery and installation, providing the warranty card is completed and returned to the Technical Services
Department.
During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship
will be repaired or replaced at Thermo's expense, labor excluded. The ULT Freezers include an additional two year warranty
on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The
Technical Services Department must be contacted for warranty determination and direction prior to any work being performed.
Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty.
In addition to the standard warranty, the foamed-in-place cabinet design carries a unit production lifetime warranty (foamed-in-
place cabinet, evaporator and foamed-in-place door; parts only). Please contact your sales representative or Thermo for addi-
tional information.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equip-
ment or to the component part beyond the original two year warranty period. The Technical Services Department must give
prior approval for the return of any components or equipment.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation, and preventive maintenance.
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Appendix A Handling Liquid Nitrogen
Warning Contact of liquid nitrogen or cold gas with skin or eyes may cause serious freezing (frostbite) injury. s
Handle liquid nitrogen carefully.

The extremely low temperature can freeze human flesh very rapidly. When spilled on a surface the liquid tends
to cover it completely and intimately, cooling a large area. The gas issuing from the liquid is also extremely
cold. Delicate tissue, such as that of the eyes, can be damaged by an exposure to the cold gas which would be
too brief to affect the skin of the hands or face.
Never allow any unprotected part of your body to touch objects cooled by liquid nitrogen.
Such objects may stick fast to the skin and tear the flesh when you attempt to free yourself. Use tongs to with-
draw objects immersed in the liquid, and handle the object carefully.
Wear protective clothing.
Protect your eyes with a face shield or safety goggles (safety glasses without side shields do not give adequate
protection). Always wear gloves when handling anything that is, or may have been, in immediate contact with
liquid nitrogen. Insulated gloves are recommended, but heavy leather gloves may also be used. The gloves
should fit loosely, so that they can be thrown off quickly if liquid should splash into them. When handling liq-
uid in open containers, it is advisable to wear high-top shoes. Trousers (which should be cuffless if possible)
should be worn outside the shoes.
The safe handling and use of liquid nitrogen in cryogenic refrigerators and dewar flasks is largely a matter of
knowing the potential hazards and using common-sense procedures based on that knowledge. There are two
important properties of liquid nitrogen that present potential hazards:
1. It is extremely cold. At atmospheric pressure, liquid nitrogen boils at -320°F (-196°C).
2. Very small amounts of liquid vaporize into large amounts of gas. One liter of liquid nitrogen becomes 24.6
cu. ft. (700l) of gas.
The safety precautions in this booklet must be followed to avoid potential injury or damage which could result
from these two characteristics. Do not attempt to handle liquid nitrogen until you read and fully understand the
potential hazards, their consequences, and the related safety precautions. Keep this booklet handy for ready
reference and review.
Note Because argon is an inert gas whose physical properties are very similar to those of nitrogen, the precautions
and safe practices for the handling and use of liquid argon are the same as those for liquid nitrogen. s
Use only containers designed for low temperature liquids.

Cryogenic containers are specifically designed and made of materials that can withstand the rapid changes and
extreme temperature differences encountered in working with liquid nitrogen. Even these special containers
should be filled SLOWLY to minimize the internal stresses that occur when any material is cooled. Excessive
internal stresses can damage the container.
Do not cover or plug the entrance opening of any liquid nitrogen refrigerator or dewar. Do not use any stop-
per or other device that would interfere with venting of gas.
Thermo Scientific -40C Lab Freezer A-1

These cryogenic liquid containers are generally designed to operate with little or no internal pressure.
Inadequate venting can result in excessive gas pressure which could damage or burst the container. Use only
the loose-fitting necktube core supplied or one of the approved accessories for closing the necktube. Check the
unit periodically to be sure that venting is not restricted by accumulated ice or frost.
Use proper transfer equipment.
Use a phase separator or special filling funnel to prevent splashing and spilling when transferring liquid nitro-
gen into or from a dewar or refrigerator. The top of the funnel should be partly covered to reduce splashing.
Use only small, easily-handled dewars for pouring liquid. For the larger, heavier containers, use a cryogenic liq-
uid withdrawal device to transfer liquid from one container to another. Be sure to follow instructions supplied
with the withdrawal device. When liquid cylinders or other large storage containers are used for filling, follow
the instructions supplied with those units and their accessories.
Do not overfill containers.
Filling above the bottom of the necktube (or specified maximum level) can result in overflow and spillage of
liquid when the necktube core or cover is placed in the opening.
Never use hollow rods or tubes as dipsticks.
When a warm tube is inserted into liquid nitrogen, liquid will spout from the top of the tube due to gasifica-
tion and rapid expansion of liquid inside the tube.
Warning Nitrogen Gas Can Cause Suffocation Without Warning! s
Store and use liquid nitrogen only in a well-ventilated place.

As the liquid evaporates, the resulting gas tends to displace the normal air from the area. In closed areas, exces-
sive amounts of nitrogen gas reduce the concentration of oxygen and can result in asphyxiation. Because nitro-
gen gas is colorless, odorless and tasteless, it cannot be detected by the human senses and will be breathed as if
it were air. Breathing an atmosphere that contains less than 18% oxygen can cause dizziness and quickly result
in unconsciousness and death.
Note The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture; not the gas
itself. The issuing gas is invisible. s
Never dispose of liquid nitrogen in confined areas or places where others may enter.
Disposal of liquid nitrogen should be done outdoors in a safe place. Pour the liquid slowly on gravel or bare
earth where it can evaporate without causing damage. Do not pour the liquid on pavement.
Appendix B Handling Liquid CO2

Warning High concentrations of CO2 gas can cause asphyxiation! OSHA Standards specify that employee
exposure to carbon dioxide in any eight-hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure limit for 15 minutes or less is 30,000
PPM (3% CO2). Carbon dioxide monitors are recommended for confined areas where concentrations of carbon
dioxide gas can accumulate. s
A-2 -40C Lab Freezer Thermo Scientific

Store and use liquid CO2 only in a well-ventilated place.
As the liquid evaporates, the resulting gas tends to displace the normal air from the area. In closed areas, exces-
sive amounts of CO2 gas reduce the concentration of oxygen and can result in asphyxiation. Because CO2 gas
is colorless, odorless and tasteless, it cannot be detected by the human senses and will be breathed as if it were
air. Breathing an atmosphere that contains less than 18% oxygen can cause dizziness and quickly result in
unconsciousness and death.
Note The cloudy vapor that appears when liquid CO2 is exposed to the air is condensed moisture; not the gas
itself. The issuing gas is invisible. s
Never dispose of liquid CO2 in confined areas or places where others may enter.
Disposal of liquid CO2 should be done outdoors in a safe place. Pour the liquid slowly on gravel or bare earth
where it can evaporate without causing damage. Do not pour the liquid on pavement.
Appendix C First Aid

If a person seems to become dizzy or loses consciousness while working with liquid nitrogen or carbon dioxide,
move to a well-ventilated area immediately. If breathing has stopped, apply artificial respiration. If breathing is
difficult, give oxygen. Call a physician. Keep warm and at rest.
If exposed to liquid or cold gas, restore tissue to normal body temperature (98.6° F) as rapidly as possible,
followed by protection of the injured tissue from further damage and infection. Remove or loosen clothing that
may constrict blood circulation to the frozen area. Call a physician. Rapid warming of the affected part is best
achieved by using water at 108° F. Under no circumstance should the water be over 112° F, nor should the
frozen part be rubbed either before or after rewarming. The patient should neither smoke nor drink alcohol.
Thermo Scientific -40C Lab Freezer A-3

A-4 -40C Lab Freezer Thermo Scientific
Thermo Fisher Scientific
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United States
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Revco® DxF -40C Upright Laboratory Freezer

Operating Manual 7008813 Rev. 1

Visit us online to register your warranty

Important installer and user information:

Models covered by this manual Packing List

Catalog Capacity - Part Number Description Quantity

DxF24040A 8813 13 120/60 34040 Key Ring 1

DxF24040V 8814 13 230/50 122005 Key 2

DxF24040D 8816 13 230/60 380520 Neoprene Cap 2

DxF32040A 8817 17 120/60 510016 1/4-20 x 5-1/2” Bolt 2

DxF32040V 8819 17 230/50 195763 Retaining Clip 1

DxF32040D 8820 17 230/60 370563 Remote Alarm Connector 1

DxF40040V 8822 23 230/50

DxF40040D 8823 23 230/60

MANUAL NUMBER 7008813

1 FR-2202 8/2/11 Updated refrigeration schematics w/ new captube length ccs

Thermo Scientific -40C Lab Freezer i

©2011 Thermo Fisher Scientific. All rights reserved.

ii -40C Lab Freezer Thermo Scientific

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientific -40C Lab Freezer iii

Do You Need Information or Assistance on

Thermo Fisher Scientific

International customers, please contact your local Thermo Scientific distributor.

iv -40C Lab Freezer Thermo Scientific

Section 2 Calibrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Section 3 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Section 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Thermo Scientific -40C Lab Freezer v

Section 5 Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Section 6 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Section 7 Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Section 8 Refrigeration Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Section 9 Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Section 10 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Section 11 Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

vi -40C Lab Freezer Thermo Scientific

Thermo Scientific -40C Lab Freezer 1-1

Optional Miscellaneous Accessories Probe

Figure 1-4. Vacuum Relief Port & Probe Cover Locations

1-2 -40C Lab Freezer Thermo Scientific

Alarm Silence Alarm

Up & Down Enter

Figure 1-6. Control Panel

Thermo Scientific -40C Lab Freezer 1-3

Control Panel • Temperature Display - Displays temperature in degrees Celsius.

• Alarm Indicator - Light pulses on/off during an alarm condition of the

• Alarm Panel - indicates the current alarm condition.

• Up and Down Arrows - Increase or decrease values, toggle between

• Enter - Stores the changed value into memory.

Figure 1-7. Basic Modes

1-4 -40C Lab Freezer Thermo Scientific

Caution For proper ventilation and airflow, a minimum clearance of 5” at

Thermo Scientific -40C Lab Freezer 1-5

IMPORTANT USER INFORMATION

Figure 1-8. Remote Alarms Contacts