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Page 1 Sample Paper 18 CBSE Biology Class 12
Sample Paper 18
Class XII 2024-25
Biology (044)
Time: 3 Hours Max. Marks: 70
General Instructions:
1. All questions are compulsory.
2. The question paper has five sections and 33 questions. All questions are compulsory.
3. Section—A has 16 questions of 1 mark each; Section—B has 5 questions of 2 marks each; Section—C
has 7 questions of 3 marks each: Section—D has 2 case-based questions of 4 marks each; and Section—E
has 3 questions of 5 marks each.
4. There is no overall choice. However, internal choices have been provided in some questions. A student
has to attempt only one of the alternatives in such questions.
5. Wherever necessary, neat and properly labeled diagrams should be drawn.
SECTION - A
Q. No. 1 to 12 are multiple choice questions. Only one of the choices is correct. Select and write the correct
choice as well as the answer to these questions.
1. Which of the following is a non-symbiotic biofertiliser?

(a) Nostoc (b) Azotobacter
(c) Anabaena (d) Rhizobium
2. Connel’s field experiment showed that on the rockysea coasts of Scotland, larger barnacle Balanus
dominates the intertidal area and removes the smaller barnade Chathamalus. This happened due to
(a) parasitism (b) predation
(c) mutualism (d) competition
3. In assisted reproductive technology, IVF involves transfer of

(a) ovum into the fallopian tube
(b) zygote into the fallopian tube
(c) zygote into the uterus
(d) embryo with 16 blastomeres into the fallopian tube.
4. Pyramid of biomass for a grazing food chain represents

(a) gradual decrease in biomass from apex to base
(b) gradual decrease in biomass from producers to the tertiary consumers
(c) gradual increase of the biomass from producers to the tertiary consumers
(d) no change in biomass
5. In a dihybrid cross, if you get 9:3:3:1 ratio it denotes that

(a) the alleles of two genes are interacting with each other
(b) it is a multigenic inheritance
(c) it is a case of multiple allelism
(d) the alleles of two genes are segregating independently.
6. The annual net primary productivity of the whole biosphere is approximately

(a) 150 billion tons (b) 160 billion tons
(c) 170 billion tons (d) 180 billion tons
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7. Match column I with column II and select the correct option from the given codes.
Column I Column II
A. Glomus (i) Mosquitoes
B. Bacillus thuringiensis (ii) Phosphorus nutrition
C. Root nodules (iii) Leghaemoglobin
D. Ladybird (iv) Bioinsecticide
(a) A-(iii), B-(i), C-(ii), D-(iv) (b) A-(ii), B-(iii), C-(iv), D-(i)
(c) A-(ii), B-(iv), C-(iii), D-(i) (d) A-(iii), B-(iv), C-(ii), D-(i)
8. What is the criterion for movement of DNA fragments on agarose gel during gel electrophoresis?
(a) The smaller the fragment size, the farther it moves.
(b) Positively charged fragments move to farther end.
(c) Negatively charged fragments do not move.
(d) The larger the fragment size, the farther it moves.
9. Maximum species diversity is seen in latitudinal range of

(a) 23.5° N to 66.5° N (b) 23.5° N to 23.5° S
(c) 23.5° S to 66.5° S (d) 66.5° N to 90° N
10. Organic farming includes

(a) use of fertilisers and pesticides of biological origin
(b) IPM (Integrated Pest Management)
(c) use of green manures
(d) all of these
11. During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates
out
(a) polysaccharides (b) RNA
(c) DNA (d) histones
12. Match column I with column II and select the correct option from the given codes.
Column I Column II
A. Sigma factor (i) 5l - 3l
B. Capping (ii) Initiation
C. Tailing (iii) Termination
D. Coding strand (iv) 5l end
(v) 3l end
(a) A-(iii), B-(v), C-(iv), D-(ii) (b) A-(ii), B-(iv), C-(v), D-(i)
(c) A-(ii), B- (iv), C-(v), D-(iii) (d) A-(iii), B-(v), C-(iv), D-(i)
13. Assertion : In barrier methods, ovum and sperms are prevented from physical meeting.
Reason : Barrier methods are used during coitus, to prevent the entry of ejaculated semen into the female
reproductive tract.
(a) Both A and R are true and R is the correct explanation of A.
(b) Both A and R are true and R is not the correct explanation of A.
(c) A is true but R is false.
(d) A is false but R is true.
14. Assertion : If the species-area relationships are analysed among very large areas like the entire continents,
the value of Z i.e., slope of line lies in the range of D.1 to 0.2.
Reason : The value of Z for frugivorous birds and mammals in the tropical forests is found to be 1.15.
15. The given below figures show the different modification found in Bougainvillea and Cucurbita. Observe
the figures carefully and comment upon the appropriateness of the Assertion and Reason.
Assertion : The given structures X and Y show convergent evolution.

Reason : Structures X and Y are modified stems that perform different functions.
16. Assertion : Ex-albuminous seeds do not possess any residual endosperm, as it is completely consumed
during embryo development.
Reason : Wheat, castor, pea and groundnut all are examples of ex-albuminous seeds.
SECTION - B
17. Mention one application for each of the following :
(i) Passive immunisation
(ii) Antihistamine
O
Mention one application for each of the following :
(i) Colostrum
(ii) Cytokinin-barrier
18. Refer to the given figure and answer the following questions:
(i) One of the sperms is observed to penetrate ‘A’ of the ovum, as shown in the above diagram. Name
‘A’.
(ii) Why only one sperm can penetrate and fertilise the ovum?
19. (i) How can we find whether an E. coli bacterium has transformed or not when a recombinant DNA
bearing ampicillin resistant gene is transferred into it?
(ii) What does the ampicillin resistant gene act as in the above case?
20. In snapdragon a cross between true-breeding red flowered (RR) plants and true-breeding white flowered
(rr) plants showed a progeny of plants with all pink flowers.
(i) The appearance of pink flowers is not known as blending. Why?
(ii) What is this phenomenon known as?
21. (i) How many primary producers do you think will be needed to support six tertiary consumers in a
grassland ecosystem?
(ii) Draw a grassland pyramid to substantiate your answer.
O
Define the following terms :
(i) Productivity
(ii) Humification
SECTION - C
22.
(i) Identify the polarity from a to a l in the above diagram and mention how many more amino acids are
expected to be added to this polypeptide chain.
(ii) Mention the DNA sequence coding for serine and the anticodon of tRNA for the same amino acid.
(iii) Why are some untranslated sequence of bases seen in mRNA coding for a polypeptide? Where exactly
are they present on mRNA?
23. Explain the events in a normal woman during her menstrual cycle on the following days:
(i) Ovarian event from 13-15 days
(ii) Ovarian hormones level from 16 to 23 days
(iii) Uterine events from 24 to 29 days
24. Describe the characteristic features of wind pollinated flowers.
25. (i) Describe Hardy-Weinberg equilibrium.

(ii) List any four factors which affect genetic equilibrium.
26. (i) Identify the molecule shown and the site labelled ‘A’.
(ii) Why is this molecule referred to as H2L2? Explain.

(iii) Mention the chemical nature of given molecule and type of cells they are produced by?
O
Give the scientific name of the parasite that causes malignant malaria in humans. At what stage does this
parasite enter the human body? Trace its life cycle in human body.
27. A segment of foreign DNA and that of a vector DNA are cut with restriction endonuclease to form a
recombinant DNA. Show with the help of schematic diagrams.
(i) The set of palindromic nucleotide sequence of base pairs the EcoRI will recognise in both the DNA
segments. Mark the site at which EcoRI will act and cut both the segments.
(ii) Sticky ends formed on both the segments where the two DNA segments will join later to form a
recombinant DNA.
28. There are many animals that have become extinct in the wild but continue to be maintained in Zoological
parks.
(i) What type of biodiversity conservation is observed in this case?
(ii) Explain any other two ways which help in this type of conservation.
SECTION - D
Question No. 29 and 30 are case based questions. Each question has subparts with internal choice in one subpart.
29. Study the schematic representation of the genes involved in the lac operon given below and answer the
questions that follow:
(i) Identify and name the regulatory gene in this operon.

(ii) What is the role of inducer in the given figure?
(iii) Why is the lac operon’s regulation referred to as negative regulation?
O
(iv) Name the inducer molecule and the products of the genes ‘z’ and ‘y’ of the operon. Write the functions
of these gene products.
30. Study the diagram showing replication of HIV in humans and answer the following questions accordingly.
(i) Write the chemical nature of the coat ‘A’.

(ii) Name the enzyme ‘B’ acting on ‘X’ to produce molecule ‘C’. Name ‘C’.
(iii) Mention the name of the host cell ‘D’ the HIV attacks first when it enters into the human body. How
does HIV differ from a bacteriophage
O
(iv) Name the two different cells the new viruses ‘E’ subsequently attack. What are two ways of transmission
of HIV infection in humans other than sexual contact.
SECTION - E
31. Mention the site of fertilisation of a human female. List the events that follow in sequence until the
implantation of the blastocyst.
O
Write briefly the role of pollination in the growth and development in an angiosperm.
32.
(i) Write your observations on the variations seen in the Darwin’s finches shown above.
(ii) Explain what conclusions did he draw and how.
O
(i) What do these pictures ‘a’ and ‘b’ illustrate with reference to evolution? Explain.
(ii) How does industrial melanism support Darwin’s theory of natural selection? Explain.
33. Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant
vector molecule cannot be created.
(i) How are the desirable DNA sequences cut?
(ii) Explain the technique used to separate the cut fragments.
(iii) How are the resultant fragments joined to the vector DNA molecule?
O
The clinical gene therapy is given to a 4-years old patient for an enzyme which is crucial for the immune
system to function. Observe the given flow chart of gene therapy and answer the following questions.
(i) Identify the disease to be cured with gene therapy.

(ii) Why the above method is not a complete solution to the problem?
(iii) Mention a possible permanent cure for this disease.
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Page 1 Sample Paper 18 CBSE Biology Class 12

1. Which of the following is a non-symbiotic biofertiliser?

3. In assisted reproductive technology, IVF involves transfer of

4. Pyramid of biomass for a grazing food chain represents

5. In a dihybrid cross, if you get 9:3:3:1 ratio it denotes that

6. The annual net primary productivity of the whole biosphere is approximately

Continue on next page.....

9. Maximum species diversity is seen in latitudinal range of

10. Organic farming includes

Assertion : The given structures X and Y show convergent evolution.

Continue on next page.....

24. Describe the characteristic features of wind pollinated flowers.

25. (i) Describe Hardy-Weinberg equilibrium.

(ii) Why is this molecule referred to as H2L2? Explain.

(i) Identify and name the regulatory gene in this operon.

(i) Write the chemical nature of the coat ‘A’.

Continue on next page.....

Continue on next page.....

(i) Identify the disease to be cured with gene therapy.

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