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    Lab.5. FOV

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    Lab.5. FOV

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    4 views35 pages

    Lab.5. FOV

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    udjhkfkg
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    You are on page 1of 35

    Lab # 5

    Determination of the
    diameter of field of view
    (dFOV)

    1 1st year- Physiology Lab


    Objectives

    • By the end of this Experiment, the student should be able


    to:
    • Determine the diameter of field of view by using ruler under 4x
    & 10x objective lenses .
    • Determine the diameter of FOV for medium & high-power lens
    (using equation)..
    • Describe the conversion of scales from cm to mm and µm.
    • Calculate the size of an object ( Cell etc.)

    2 1st year- Physiology Lab


    Outlines

    • Definition- field of view.


    • Units of measurement.
    • conversion units.
    • How to measure diameter field of view.

    3 1st year- Physiology Lab


    Requirements

    small transparent metric ruler


    Compound light microscope

    4 1st year- Physiology Lab


    Diameter of Field of view

    5 1st year- Physiology Lab


    Theory and Principle
     Field of view is the diameter of the circle of light that you see when
    you look through the eye pieces

    (If you know how wide your field of view is, you can estimate the size
    of things you see in the field of view)

    As magnification increase the FOV get smaller (decrease )

    6 1st year- Physiology Lab


    For microscopic measurement of the size and diameter of
    an object, we usually use metric system Which is based on
    the ‘meter

    But when we use a MICROscope we tend to use


    MICROmeters.

    To convert from millimeters to micrometers →

    7 1st year- Physiology Lab


    8 1st year- Physiology Lab
    Units

    ➢ Visual :
    • 1 meter (m) =100 centimeter (cm)
    or 1000 millimeter (mm)

    • 1 cm =10 mm
    • 1 mm =1000 micrometer (µm)
    ➢ Microscopic
    • 1 µm =1000 nanometer (nm)
    • 1 nm =10 angstrom (A0)
    Most cells are between 1 and 100 µm in diameter and
    therefore are visible only under the microscope.
    9 1st year- Physiology Lab
    conversion of units
    ÷ 10 cm x 10

    mm 10mm

    ÷ 1000 x 1000

    µm 10,000µm

    ÷ 1000 x 1000

    nm 10,000,000nm
    10 1st year- Physiology Lab
    Measure diameter of field of view (dfov)
    dFOV (mp) = diameter of field of view of medium power lens (..mm)

    dFOV (hp) = diameter of field of view of high-power lens (…mm)

    dFOV (lp) = diameter of field of view of low power lens (…mm)

    Mag. lp = magnification power of low power lens (10x)

    Mag.mp = magnification power of medium power lens (40x)

    Mag.hp = magnification power of high-power lens (100x)

    11 1st year- Physiology Lab


    Measure diameter of field of view (dfov)
     Two steps:
    ➢ Using ruler under 4x and 10x only

    ➢ Using equation (formula) for 40x and 100x

    dFOVmp = dFOVlp x Mag.lp


    Mag.mp

    dFOVhp = dFOVlp x Mag.lp


    Mag.hp
    12 1st year- Physiology Lab
    4x
    By Ruler Scanning

    dfov(4x)= 5mm
    13 1st year- Physiology Lab
    1mm

    X Correct

    14 1st year- Physiology Lab


    By Ruler 10x
    Low Power

    10

    dfovlp= 1.5mm

    10x (1.5mm)
    15 1st year- Physiology Lab
    Finding Field of View (F.O.V)

    Under Low Power(10X): Use millimeter ruler

    • Diameter of field is 1.5 mm →

    • 1000 × 1.5 mm = 1500 micrometers

    16 1st year- Physiology Lab


    Finding Field of View (F.O.V)

    ❖Under Medium or High Power: provide


    a field of view that is less than 1 mm in
    diameter, so it cannot be measured with a
    ruler

    So, How to measure ….

    ❑Remember!!
    As magnification increases FOV
    17
    decreases
    1st year- Physiology Lab
    By equation:

    dfovmp for 40x

    and dfovhp for 100x

    40x, 100x
    18 1st year- Physiology Lab
    4x 10x
    Scanning Low Power 4,10, 40x, 100x

    4x (5mm) 10x (1.5mm)

    dfov4x= 5mm dfovlp= 1.5mm dfovmp By equation

    By Ruler
    19 1st year- Physiology Lab
    Diameter of field of view by ruler

    dfov4x = ? ___________

    dfovlp = ? ___________

    Diameter of field of view by formula (equation)

    dfovmp = ? __________

    dfovhp = ? __________

    20 1st year- Physiology Lab


    Calculation (exercise for students)
    • dfovlp = 1.5mm (ruler)
    • Mag.lp = 10x
    dFOVmp = dFOVlp x Mag.lp
    Mag.mp

    • Mag.mp = 40x
    • dfovmp. 0. 375mm (equation)
    dFOVhp = dFOVlp x Mag.lp
    Mag.hp

    • Mag.hp = 100x
    • dfovhp. = 0. 15 mm (equation)

    21 1st year- Physiology Lab


    • For microscopic measurements micrometers are converted
    into millimeters as……….

    a. quantities of micrometer ÷ 1000


    b. quantities of micrometer × 1000
    c. quantities of micrometer - 1000
    d. quantities of micrometer + 1000

    22 1st year- Physiology Lab


    a) Write the formula used for the determination of diameter of
    FOV for high power lens.

    23 1st year- Physiology Lab


    Exercise

    • dfovlp = 2.5mm
    0.625mm 625 um
    • dfovmp = ?

    dFOVmp = dFOVlp x Mag.lp


    Mag.mp

    24 1st year- Physiology Lab


    Solution

    • dfovlp =2.5mm dfovmp = ?


    • Mag.lp =10x
    • Mag.mp = 40
    dFOVmp = dFOVlp x Mag.lp
    Mag.mp

    • 2.5 x 10 / 40
    • 0.625
    • 0.625mm x 1000=625um

    25 1st year- Physiology Lab


    Exercise

    • dfovlp = 2.5mm
    0.25 mm 250 um
    • dfovhp = ?

    dFOVhp = dFOVlp x Mag.lp


    Mag.hp

    26 1st year- Physiology Lab


    Solution

    • dfovlp =2.5mm dfovhp = ?


    • Mag.lp =10x
    • Mag.hp = 100
    dFOVhp = dFOVlp x Mag.lp
    Mag.hp
    • 2.5 x 10 / 100
    • 0.25
    • 0.25mm x 1000 = 250um

    27 1st year- Physiology Lab


    Calculate the size of an object
    or cell

    28 1st year- Physiology Lab


    Calculate size of an object/cell

    1st step : Determine dfovlp (use ruler)

    2nd step : Determine dfovmp (use equation)


    dFOVmp = dFOVlp x Mag.lp
    Mag.mp

    3rd step : Calculate the size of an object


    (dfovmp/no. of object under microscope)
    29 1st year- Physiology Lab
    Diameter field of view for medium-power (40x) =

    Diameter field of view for low power x Magnification of low power


    Magnification of medium power

    OR

    dFOVmp = dFOVlp x Mag.lp


    Mag.mp

    30 1st year- Physiology Lab


    On Medium power,

    dFOVmp .

    31 1st year- Physiology Lab


    Medium Power 40x
    dFOVmp,

    32 1st year- Physiology Lab


    Medium power = 40x
    Conclusion
    dfovmp / no. of Object/cell

    Medium Power 40x

    33 1st year- Physiology Lab


    References

    1) Ross and Wilson Anatomy and Physiology in Health and illness Anne
    Waugh ,Allison Grant 13 th Edition,2018. Churchill Living stone, London,
    New YORK.

    1st year- Physiology Lab


    2) Laboratory Exercises and Techniques in Cell Biology by Anthony
    Contento 1st Edition 2012 Wiley publisher ,USA

    3)Principle of Human Physiology Gerald J. Torture 11th Edition.

    34
    Thank You

    35 1st year- Physiology Lab

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