4506

Download as pdf or txt
Download as pdf or txt
You are on page 1of 2

Product Information Sheet for NR-3

Escherichia coli, Strain EDL 931 Incubation:


Temperature: 37°C
Atmosphere: Aerobic
Catalog No. NR-3 Propagation:
®
(Derived from ATCC 35150™) 1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of
For research use only. Not for human use. Tryptic Soy broth.
3. Use several drops of the suspension to inoculate a
Tryptic Soy agar slant and/or plate.
Contributor:
4. Incubate the slant and/or plate at 37°C for 24 hours.
ATCC
Citation:
Manufacturer: Acknowledgment for publications should read “The following
BEI Resources reagent was obtained through BEI Resources, NIAID, NIH:
Escherichia coli, Strain EDL 931, NR-3.”
Product Description:
Bacteria Classification: Enterobacteriaceae, Escherichia Biosafety Level: 2
Species: Escherichia coli Appropriate safety procedures should always be used with
Strain: EDL 931 this material. Laboratory safety is discussed in the following
Serotype: O157:H7 publication: U.S. Department of Health and Human Services,
1
Original Source: Isolated in 1982 from human feces Public Health Service, Centers for Disease Control and
Comment: Escherichia coli, strain EDL 931 was deposited at Prevention, and National Institutes of Health. Biosafety in
®
ATCC in 1983 by Dr. George K. Morris, The Enteric Microbiological and Biomedical Laboratories. 5th ed.
Bacteriology Section, Centers for Disease Control, Atlanta, Washington, DC: U.S. Government Printing Office, 2009; see
Georgia. www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Escherichia coli (E. coli) is a Gram-negative, rod-shaped Disclaimers:
bacterium which occurs singly or in pairs. It is a major You are authorized to use this product for research use only.
facultative inhabitant of the large intestine. The O157:H7 It is not intended for human use.
serotype is associated with Hamburger disease, which is
caused by the contamination of meat products by this Use of this product is subject to the terms and conditions of
organism. the BEI Resources Material Transfer Agreement (MTA). The
MTA is available on our Web site at www.beiresources.org.
E. coli, strain EDL 931 carries the large pO157 plasmid as
well as the genes for Shiga toxins 1 and 2 that are found in While BEI Resources uses reasonable efforts to include
2
most enterohemorrhagic E. coli (EHEC) strains. accurate and up-to-date information on this product sheet,
®
neither ATCC nor the U.S. Government makes any
Material Provided: warranties or representations as to its accuracy. Citations
Each vial contains approximately 0.5 mL of bacterial culture from scientific literature and patents are provided for
®
in Tryptic Soy broth supplemented with 10% glycerol. informational purposes only. Neither ATCC nor the U.S.
Government warrants that such information has been
Note: If homogeneity is required for your intended use, confirmed to be accurate.
please colony-purify prior to initiating work.
This product is sent with the condition that you are
Packaging/Storage: responsible for its safe storage, handling, use and disposal.
®
NR-3 was packaged aseptically, in screw-capped plastic ATCC and the U.S. Government are not liable for any
cryovials. The product is provided frozen and should be damages or injuries arising from receipt and/or use of this
stored at -60°C or colder immediately upon arrival. For product. While reasonable effort is made to ensure
long-term storage, the vapor phase of a liquid nitrogen authenticity and reliability of materials on deposit, the U.S.
®
freezer is recommended. Freeze-thaw cycles should be Government, ATCC , their suppliers and contributors to BEI
avoided. Resources are not liable for damages arising from the
misidentification or misrepresentation of products.
Growth Conditions:
Media: Use Restrictions:
Tryptic Soy broth or equivalent This material is distributed for internal research, non-
Tryptic Soy agar or equivalent commercial purposes only. This material, its product or its
derivatives may not be distributed to third parties. Except as
performed under a U.S. Government contract, individuals

BEI Resources E-mail: contact@beiresources.org


www.beiresources.org Tel: 800-359-7370
Fax: 703-365-2898

© 2004/2005/2008/2015 American Type Culture Collection (ATCC). All rights reserved. NR-3_18MAR2015
Page 1 of 2
Product Information Sheet for NR-3

contemplating commercial use of the material, its products or


its derivatives must contact the contributor to determine if a
license is required. U.S. Government contractors may need
a license before first commercial sale.

References:
1. Riley, L. W., et al. “Hemorrhagic Colitis Associated with
a Rare Escherichia coli Serotype.” N. Engl. J. Med. 308
(1983): 681–685. PubMed: 6338386.
2. Downes, F. P., et al. “Development and Evaluation of
Enzyme-Linked Immunosorbent Assays for Detection of
Shiga-Like Toxin I and Shiga-Like Toxin II.” J. Clin.
Microbiol. 27 (1989): 1292–1297. PubMed: 2666439.
3. Escobar-Páramo, P. et al. “A Specific Genetic
Background Is Required for Acquisition and Expression
of Virulence Factors in Escherichia coli.” Mol. Biol. Evol.
21 (2004): 1085–1094. PubMed: 15014151.
®
ATCC is a trademark of the American Type Culture
Collection.

BEI Resources E-mail: contact@beiresources.org


www.beiresources.org Tel: 800-359-7370
Fax: 703-365-2898

© 2004/2005/2008/2015 American Type Culture Collection (ATCC). All rights reserved. NR-3_18MAR2015
Page 2 of 2

You might also like