Nikon

Microscope

ECLIPSE E600
Instructions
 Thank you for purchasing the Nikon products.
This instruction manual is written for the users of the Nikon Microscope ECLIPSE E600.
To ensure correct usage, read this manual carefully before operating the instrument.

* It is prohibited to reproduce or transmit this manual in part or whole without Nikon's ex-
pressed permission.
* The contents of this manual are subject to change without notice.
+ Although every effort has been made to ensure the accuracy of this manual, if you note any
points that are unclear or incorrect, contact your nearest Nikon representative.

Warning/Caution Symbols Used in This Manual

Although Nikon products are designed to provide you with the utmost safety during use,
incorrect usage or disregard of the instructions can cause personal injury or property damage.
For your safety, read the instruction manual carefully and thoroughly before using the instru-
ment. Do not discard this manual but keep it near the product for easy reference.
In this manual, safety instructions are indicated with the symbols shown below. Be sure to
follow the instructions indicated with these symbols to ensure correct and safe operation.

Symbol Meaning

AN ARNING Disregarding instructions marked with this symbol may lead to death
or serious injury.

CAUTION Disregarding instructions marked with this symbol may lead to injury
/ \ or property damage.
Anne

1. Intended product use

This microscope should only be used for microscopic observation. Do not use it for any other purpose.

Do not disassemble
Disassembling may cause malfunction and/or electrical shock. Do not disassemble any paris other
than those mentioned in this manual. If you notice any malfunction, notify your nearest Nikon
representative.

Input voltage
Make sure that the input voltage indicated on the
back panel of the microscope is the same as your Nikon
MODEL ECLIPSE E600
regional voltage. If not the same, do not use the 120V~
microscope; instead, notify your nearest Nikon 24A
50/60HZ
representative immediately. If the microscope is MADE IN JAPAN
used with the wrong input voltage, a short circuit
or fire may result, causing the damage of the
microscope.

Power cord
To prevent electrical shock, always turn off the
power switch (flip it to the “ *" side) before
connecting or disconnecting the power cord. Use
only the following power supply cord set. Using
the wrong power cord could result in damage or
fire.
* For 100-120V area
UL Listed, detachable cord set, 3 conductor
grounding type SVT, No. 18 AWG rated at
125V,7A minimum.
In case of using the extension cord, use only
the power supply cord with the PE (protective
earth) wire.
+ For 220- 240V area
3 pole power supply cord set, which must be
approved according to EU/EN standards.
Class I equipment should be connected to PE
(protective earth) terminal.
In case of using the extension cord, use only
the power supply cord with the PE (protective
earth) wire.

Heat from the light source

The lamp and the lamphouse become extremely
hot. To avoid burns, do not touch the lamphouse
while the lamp is lit or for thirty minutes after it
has been turned off.
Furthermore, in order to avoid the risk of fire, do
not place fabric, paper or highly flammable
materials such as gasoline, petroleum benzine,
paint thinner or alcohol near the lamphouse while
it is lit or for about thirty minutes after it has been
turned off.
The back of the microscope also becomes hot
during use. Although this is not a malfunction,
be careful not to touch the back of the microscope
when it is hot.
[Nana

1. Only use the specified halogen lamp

The power supply unit in the microscope provides the power for the halogen lamp that serves as the
microscope light source. This unit can supply power for a halogen lamp of up to 12 V-100 W. The
specified lamp and lamphouse must be used, otherwise, the microscope could be damaged.
Specified lamphouse: Nikon C-LP HALOGEN 12V100W model
Specified lamp: 12V-100W LONGLIFE halogen lamp (OSRAM HLX64623 or PHILIPS
7724)

Precautions for replacing the lamp

To prevent burns, allow the lamp to cool for at least thirty minutes after turning off the power switch
before replacing it. Furthermore, to prevent electrical shock and damage to the microscope, always
turn off the power switch (flip it to the “ > side) and unplug the power cord before connecting or
disconnecting the lamphouse.
After replacing the lamp, be sure to attach the lamphouse cover securely. Never use the lamphouse
without its cover.

Precautions for operating the stage

The stage rack protrudes when the stage is moved.
When operating the focus knobs or condenser
focus knob, be careful not to graze your hand on
the end of the rack.

Do not wet the microscope

If the microscope gets wet, a short circuit may result that could damage it or make it extremely hot.
If you accidentally spill a liquid on the microscope, immediately turn off the power switch (flip it to
the “ +” side) and unplug the power cord. Then use a dry cloth to wipe away the moisture. If any
liquid gets inside the microscope, do not use it; instead, notify your nearest Nikon representative.

Weak electromagnetic waves

This microscope emits weak electromagnetic waves. The accuracy of any precision electronic
equipment may be adversely affected if positioned too close. If the microscope affects TV or radio
reception, move the radio or TV further away from the microscope.

Precautions for installation

Be careful not to pinch your hands or fingers when installing the microscope.
This microscope is a precision optical instrument, and using or storing it under unsuitable conditions
may damage it or may have an adverse effect on its accuracy. See “Installation location” on p. 24 and
always use the microscope in a suitable environment.
A cures

7. Precautions for moving the microscope

First detach the lamphouse, and then securely hold
the microscope by the base of the arm from the
back.
This microscope is a precision optical instrument,
so handle it carefully and do not subject it to a
strong physical shock. (The accuracy of the
objective in particular may be adversely affected
by even a weak physical shock.)
In addition, do not grasp the focus knobs, eyepiece
tube, stage, etc. when carrying the microscope.
This could cause the part to come off, resulting
in damage to the microscope.
CONTENTS

Warning/Caution Symbols Used in This Manual ezine see ieenionionia 1

PAN SA 2
LS CAUTION ccoo errores 3
il Names of Component Parts and Operational PartS...........................-srerrercenerienicane: 6

H Microscobpツ tirrenia rioni rien rioniie nie iene페미 8

El OperationofEachPart..............................................
0.0000 aneen aaa enne oro rro riores 13
Coarse and fine focus knobs ii 13
1. Rotation of the coarse and fine focus knobs and
vertical movement of the stage .................. i 13
2. Adjusting the torque of the coarse focus knob nn... 13
3. Coarse focus stoDDeT e eee 14
Eyepiece tube optical path selection (T eyepiece tube, F eyepiece tube) ............... 15
1. Vertical tube adapter i 15
2. TandF eyepiece tubes lever clickind …上 せ ドー トー タート ーー 15
Diopter adjustment en 16
EJ Interpupillary distance adjustment nn 16
Condenser μμ ----,,,, 17
1. Focusing and Centering ss e Pen 17
2. Condenser aperture diaphragm eee nene een ena 0 18
3. Objectiveandceondensercombinations.................................... 19
E Field diaphragm nın 19
Filters... ИИНИ 20
EJ] Stage ОА 20
1. Adjusting the torgue ofthestagemotioncontrolknobs..................................... 20
2. Stage rotation............... issues K K RKK 21
E] Auto-photo switch (for photomicrography) ーーoooeeeeeeeeeww 21
Oil-immersion operation ............. sise 22
Water-immersion operation sers 23

ASSEMDIY ............e renne nie rerian reni can aenienera nec eee nie nieonanienisanienice seneca niaenicniene zen eve nienienina 24
1. Confirming the input voltage eee eee nn nee nen n tn 25
2. Lamp and lamphouse installation and removal (lamp replacement) ................... 26
NS EA 27
4. Revolving nosepiece assembly nr nn na nan once 28
5. Eyepiece tube assembly ii 28
6. Connecting the power cord... si 1.11 29
7. Installing separately sold accessories i 29

Troubleshooting Tables .…........................................

nn n Kne 30
Viewing and control Systems .ps 30
Electrical system see 33
[3 Care and Maintenance μμ μμ” ---ϱ------ϱϱ-ϱ--ρ--- 34
Electrical Specifications osse serene seeren renee En Eee renerne 35
ASS 36
 Names of Component Parts
and Operational Parts

If the microscope has not yet been assembled, see chapter “4. Assembly” first.

Photomicrographic
vertical tube adapter

Trinocular eyepiece tube

Diopter adjustment ring

Eyepiece

Specimen holder (AS. |

Condenser clamp ロン タジク Q (O
screw SS AS

—— > >
Condenser focus knob + Revolving nosepiece

Coarse torque adjustment

ring (TORQUE) Objective

Coarse focus knob

Fine focus knob N J

X DN
N | Stage
Brightness adjuster DN Stage rotation
(LAMP) clamp screw
|

Auto-photo switch AT |

Auto-photo voltage
selection switch
 1. Names of Component Parts and Operational Parts

Photomicrographic
equipment clamp screw

cm
selection lever

Eyepiece tube
clamp screw

Revolving nosepiece
clamp screw

Substage clamp screw

Condenser
aperture scale Filter release lever

7 > Filter insertion lever

Condenser aperture
diaphragm ring

Y-axis stage motion

Condenser control knob

Fine focus knob

AA ON

TŽ TT
=

Field diaphragm ring a Power switch

X-axis stage motion

a control knob
X|

Nikon
Curse
6600 Coarse focus stopper
Field lens ring (CLAMP)

Diffuser insertion/
removal screw

Tool holder

Rating label

Lamphouse

AC input connector

Power cord
Microscopy

The general procedure for microscopy is described below.

For details on each step, refer to the corresponding item in chapter “3. Operation of Each Part.”
If the microscope has not yet been assembled, see chapter “4. Assembly” first.

Turn on the power.

(Flip the switch to the Flip the switch to
« | ” side.) the “ | "side.
As soon as the power
comes on, the switch
lights.

Set the brightness adjuster

to give the desired
brightness.
Pressing the auto-photo
switch will set the voltage
for the brightness that
provides the best color
reproduction. (p. 21)
Adjust the
Insert filters ND 16 or 4 Insert filters.— brightness.
and NCBII into the Auto-photo switch
optical path. can be pressed.
ND 16 or 4 reduces glare in
the binocular eyepiece.
NCB11 improves color
reproduction. (p. 20)

Move the 10x objective El Set the

into the optical path. 10x objective.
Rotate the revolving
nosepiece until the 10x
objective clicks into place.

Set the specimen in place

with the cover glass facing
up.
 2. Microscopic Procedure

Raise the condenser as

high as it will go.

Fully open the field

diaphragm and condenser
| aperture diaphragm.
I Raise the —
condenser.

Fully open the condenser Fully open the

aperture diaphragm. field diaphragm.

Move the specimen until

the portion to be viewed is
in the optical path. (p. 20)

Loosen the coarse focus

stopper ring.
Rotate it in the direction
opposite the arrow on the
base as far as it will go.
(p. 14)

El Set the portion

El Loosen the coarse to be viewed in
focus stopper. the optical path.

+
 Set the optical path to
100% of the binocular
| eyepiece when using a Set the optical path
| triocular eyepiece tube. to 100% of the
2 (p.15) binocular eyepiece.

Focus on the specimen.

(p. 13)
Focus on
the specimen.

ins
o
li

Adjust the diopter rings of

both eyepieces. (p. 16)

Adjust the interpupillary

distance. (p. 16) Adjust the
diopter rings.

Adjust the
interpupillary
distance.

10
 2. Microscopic Procedure

Focus and center the

condenser. (p. 17)

|
[i Focus the LU
condenser.

Center the
e600 condenser.

Switch to any desired

objective and view the
| specimen.
Rotate the revolving
nosepiece to its click-stop
© position.

Readjust the focus.

{7A Adjust the
回 回 Switch
Swi brightness with
the ND filters.
objective.

A
Use ND filters to adjust
the brightness.
IB Readjust the =
focus.

11
 2. Microscopic Procedure

Close the field diaphragm

so that it is just outside the
EC lose th e conden
denser
field of view. (p. 19) aperture diaphragm.

Close the condenser

aperture diaphragm to
¿about 70 — 80% of the
numerical aperture of the
objective. (p. 18)

I] Close the field diaphragm.

Cura portion of the field of view is dark, adjust the condenser centering screws. If that does not resolve
the problem, check the following items:
* Filter insertion/removal (p. 20)
¢ Optical path selection for the eyepiece tube (p. 15)
* Position of the revolving nosepiece (p. 11)
* Revolving nosepiece installation (p. 28)
+ Lamp installation (p. 26)
* Condenser focusing and centering (p. 17)
+ Field and aperture diaphragms (p. 18 and 19)
¢ Condenser installation (p. 27)

M If it is not possible to focus on the specimen, check the following items:

* Mounting of the specimen (p. 31 and 32)
* Release of the coarse focus stopper (p. 14)
¢ Thickness of the cover glass (0.17 mm is standard) (p. 31 and 32)

12
 Operation of Each Part

1 Coarse and fine focus knobs

| 1. Rotation of the coarse and fine focus knobs and

vertical movement of the stage NS

Rotating the fine focus knob one step moves the stage 1 um.
Rotating the fine focus knob one complete turn moves the stage 0.1 mm.
Rotating the coarse focus knob one complete turn moves the stage 12 mm.
The coarse/fine focus stroke (range of vertical motion) for the stage is 2 mm up and 23 mm down
from the reference (focused) position.

Never attempt either of the following actions

These actions will damage the microscope.
¢ Rotating the left and right knobs in opposite
directions at the same time.
« Continuing to rotate the coarse focus knob
after the stage has reached the limit of its mo-
tion.

| 2. Adjusting the torque of the coarse focus knob BE

It is possible to adjust the torque of the coarse focus
To increase
knob. \ \ \ \ \
the torque
To increase the torque, turn the torque adjustment ring
(TORQUE) located behind the coarse focus knob in the \ \ \ \ \
direction of the arrow on the microscope base (i.e., the \ \ \ \
counter-clockwise direction). To reduce the torque, turn \ い
VA
the ring in the direction opposite to the arrow (i.e., the \
clockwise direction).

13
 3. Coarse focus stopper NS

Clamp the coarse

focus stopper

Purpose of the coarse focus stopper

The coarse focus stopper marks the stage position at which the specimen is in focus by restrict-
ing the movement of the coarse focus knob. (Movement of the stage by the fine focus knob is
not restricted.)
Once the coarse focus stopper has been clamped in position, the coarse focus knob cannot be
used to move the stage any higher. In effect, once the coarse focus knob has been clamped in
place at the focus position, a rough focus can be attained the next time simply by turning the
coarse focus knob as far as it will go. This feature is convenient when viewing similar speci-
mens one after another.

Using the coarse focus stopper

With the specimen in focus, turn the coarse focus stopper ring as far as it will go in the direction
of the arrow on the base of the microscope (about 3/4 revolution). The coarse focus stopper is
now clamped in position.
When changing the specimen, lower the stage by turning only the coarse focus knob.
After changing the specimen, gently raise the stage by turning only the coarse focus knob as far
as it will go.
The specimen should be roughly in focus when the stage has been raised as far as it will go; use
the fine focus knob to bring the specimen into perfect focus.
If the coarse focus stopper is not being used, be sure to turn the coarse focus stopper ring in the
direction opposite to the arrow on the microscope base as far as it will go.

14
 3. Operation of Each Part

2 Eyepiece tube optical path selection

= (T eyepiece tube, F eyepiece tube)

The optical path selection lever can be used to select the way to divide the amount of light between
the binocular part and the vertical tube.
Light proportion Light proportion
Lever binocular vertical Lever binocular vertical
position part tube position part tube
Ш 100 : ο
FAT] sino—-1] 100 : 0 / „a 0 : 100
BINO&
F- PHOTOT [2] 20 : 80 o em 00
FEI PHOTO- 0 : 100 BINO PHOTO

Tİ
13]
+ PS
T eyepiece tube F eyepiece tube

Vi. vertical tube adapter

A photomicrographic vertical tube adapter is pro-
vided as standard equipment that allows a photomi-
crographic equipment to be installed. To install the
adapter, insert it into the vertical tube and clamp
three screws with the provided screwdriver. Re-
place this adapter with the optional TV vertical tube
adapter when using a TV camera.

2. T and F eyepiece tubes lever clicking

There is a switch identified by “NO CLICK” on the
bottom surface of the eyepiece tube. Turn the switch
in the direction of the arrow with the hexagonal
screwdriver provided to disengage the clicking ac-
tion of the optical path selection lever. Disengag-
ing the clicking action minimizes small vibrations
produced by operating the lever.

15
 3 Diopter adjustment

xd Engraved line

=
Diopter adjustment "0" position '

Diopter adjustment compensates for differences in eyesight between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the difference
in focus when changing the objectives. Adjust the diopter setting for both eyepieces.

1 Turn each diopter adjustment ring on both eyepieces to align the edge of the ring with the
engraved line. This is the “O” position.
2 Perform steps MJ to IJ in chapter “2. Microscopic Procedure” so that the specimen is in focus
with the 10x objective.
3 Move the 40x objective into the optical path. Turn the coarse and fine focus knobs to focus
on the specimen.
4 Move the 4x or 10x objective into the optical path.
5 Turn the diopter adjustment ring on each eyepiece, not the coarse and fine focus knobs,
to bring the specimen into focus, while peering through the right eyepiece with your right
eye and the left eyepiece with your left eye.
6 Repeat steps 3 to 5 twice.

Interpupillary distance adjustment

Before adjusting the interpupillary distance, performs steps MN to KE in chapter “2. Microscopic
Procedure” so that the specimen is in focus with the 10x objective.
Adjust the interpupillary distance so that the view field for each eye is at the same position on the
specimen.
Doing so will make observation through the binocular eyepiece with both eyes easier.

16
 3. Operation of Each Part

っ Condenser

1. Focusing and centering

Eyepiece view field yepiece view field

Focus and center the condenser so that the light passing through the condenser forms the image at
the correct position on the specimen (i.e., at the center of the optical path).
Before focusing and centering the condenser, perform steps I to KE] in chapter “2. Microscopic
Procedure” so that the specimen is in focus with the 10x objective.

Close the field diaphragm to its minimum setting.

N 一

Turn the condenser focus knob so that the image of the field diaphragm is formed on the
specimen.
Make rough adjustments with the condenser centering screws so that the image of the field
diaphragm appears at the center of the eyepiece view field.
Move the 40x objective into the optical path. Turn the fine focus knob to focus on the
specimen.
Turn the condenser focus knob so that the image of the field diaphragm is formed on the
specimen.
Adjust the condenser centering screws so that the image of the field diaphragm appears at the
center of the eyepiece view field. This adjustment is easier to make if you adjust the size of
the field diaphragm to be slightly smaller than the eyepiece view field.

17
 § 2. Condenser aperture diaphragm

Numerical
Plan 40X _ Aperture
40x /:0.75° RE
© -WD 22

0.75X0.7~0.8=0.525~0.6

The aperture diaphragm is important because it is related to the resolution, contrast, depth of focus
and brightness of the optical image. Turning the condenser aperture diaphragm ring changes the
size of the aperture diaphragm.
As the aperture diaphragm is stopped down, resolution and brightness are reduced while contrast
and depth of focus are increased. Conversely, as the aperture diaphragm is opened, resolution and
brightness are increased while contrast and depth of focus are reduced. It is not possible to adjust
one pair of characteristics without affecting the other. Generally, a satisfactory image with appro-
priate contrast can be obtained with an aperture setting that is 70% to 80% of the numerical aperture
of the objective. The numerical aperture is indicated on the barrel of each objective.
An indication of 40x/0.75 means that the magnification is 40x and the numerical aperture is
0.75.
If the aperture diaphragm is stopped down too far, the resolution is reduced; therefore, except when
viewing a nearly transparent specimen, we do not recommend stopping down the aperture to less
than 60% of the numerical aperture of the objective.

Adjusting the size of the aperture diaphragm according to the condenser scale
Since the condenser scale indicates the numerical aperture, adjust the aperture diaphragm ring
according to the scale. (Normally, the index on the aperture diaphragm ring should be aligned
with the scale line corresponding to 70% to 80% of the numerical aperture of the objective.)

Adjusting the size of the aperture diaphragm by using a centering telescope

(sold separately)
Remove one of the eyepieces and, by using a special adapter (sold separately), mount a centering
telescope in its place. Turn the aperture diaphragm ring to stop down the aperture diaphragm as
far as possible. While holding down the flange of the telescope, turn the eyepiece of the center-
ing telescope and focus on the aperture diaphragm.
Turn the aperture diaphragm ring to adjust the size of the aperture diaphragm. (Normally, the
aperture diaphragm should be adjusted so that it is about 70% to 80% of the size of the field
being viewed.)
Remove the centering telescope and adapter, and then re-fit the eyepiece.

18
 3. Operation of Each Part

3. Objective and condenser combinations

|
C -
ondenser та Swing-out Achromat Abbe 00 .
Objective aplana condenser condenser condenser magnification
magnification condenser condenser
1x x x x x
2x x x x © (Note 2)
© (Note 3)
4x x O (Note 1) | O (Note 1)
10x to 100x © O O O x
© :Optimum ©: Appropriate — X: Inappropriate

Note 1: The entire view field may not be satisfactory when a UW eyepiece is used.
Note 2: Indoor lighting and similar light sources may be reflected from the surface of the condenser
lens and be visible within the view field. When this happens, either dim the indoor lighting
or prevent strong outside light from entering the stage.
Note 3: Swing out the top lens before usage.

¢ Depending on the type of objective, the whole numerical aperture of the objective may not be
covered. For example, when an objective with an N.A. of 1.4 is used, the aperture size of the
swing-out condenser or the abbe condenser will only be about 65% of the objective's N.A. even
when it is fully opened.
* Refer to the condenser instruction manual for details of universal and phase contrast condensers.

6 Field diaphragm
The field diaphragm restricts illumination to the area on the specimen being viewed. Turning the
field diaphragm ring changes the size of the field diaphragm. For normal observation, the size of the
diaphragm should be slightly larger than the boundary of the view field. If a broader area than
necessary is illuminated, stray light will enter the view field, creating flaring and reducing the con-
trast of the optical image. The correct setting of the field diaphragm is especially important in
photomicrography; generally, good results are obtained by stopping down the field diaphragm to
just slightly larger than the area that will be reproduced on the film, i.e., the size of the photo frame.

19
 7 | Filters

Three filters are housed in the base of the microscope.

A filter is inserted into the optical path by pressing in the filter insertion lever on the right side of the
microscope. The filter is removed from the optical path by pressing the release lever down.

NCB11 (color balancing filter) | For color balance adjustment and color photomicrography
ND16 (transmission rate: 6%) | For brightness adjustment
ND4 (transmission rate: 25%) | For brightness adjustment

A diffuser is contained in the microscope. When removing the diffuser from the optical path, turn
the diffuser insertion/removal screw as far as it will go (about 90°) in the counter-clockwise direc-
tion with a hexagonal screwdriver. When returning the diffuser to the optical path, turn the screw as
far as it will go in the clockwise direction.

| 8 Stage

1. Adjusting the torque of the stage motion control knobs [HH

The torque of the X-axis and Y-axis stage motion control knobs can be adjusted.

Adjusting the torque for Y-axis stage motion

To increase the torque of the Y-axis, turn Knob B
in the direction of arrow “a” while holding Knob Knob A
A; to reduce the torque, turn Knob B in the oppo-
site direction. Knob B

Adjusting the torque for X-axis stage motion a à

il
To increase the torque of the X-axis, turn Knob C
in the direction of arrow “b” while holding Knob You
D; to reduce the torque, turn Knob C in the oppo- 1011 b Knob C
site direction. Tri

Knob D

20
 3. Operation of Each Part

2. Stage rotation
Loosen the stage rotation clamp screw to rotate the stage. Rotating the stage is useful for cropping
images during photomicrography.

CAUTION The stage rack protrudes when the stage is

moved. When operating the focus knobs or
condenser focus knob, be careful not to graze
your hand on the end of the rack.

9 i Auto-photo switch (for photomicrography)

The color temperature of the lamp varies according to the voltage. If the voltage is high, the color
temperature of the lamp increases and the light becomes bluer. If the voltage is low, the color
temperature of the lamp decreases and the light becomes redder. Therefore, to obtain the best color
reproduction in color photomicrography, it is necessary for the lamp voltage to be kept constant.
When using daylight-type color film, the standard setup is to use the color balancing filter (NCB1 1)
and set the lamp voltage to 9 V.
The auto-photo switch is used to automatically set the stan-
dard lamp voltage (9 V). If the images on color film shot
with the auto-photo switch on are reddish or bluish, finely
adjust the voltage with the auto-photo voltage selection
switch. The center position of the 5-level slide switch is
roughly 9 V. Sliding the switch forward increases the blu-
ish tint of the light, while sliding the switch towards the
back increases the reddish tint of the light.
Use commercially available color compensation filters (CC
filters) if this adjustment does not resolve the problem.

21
 LU oil-immersion operation
o (using an oil-immersion type of objective and condenser)

An objective marked “Oil” is an oil-immersion type. These objectives are used with the immersion
oil applied between the specimen and the tip of the objective. The immersion oil is provided for the
microscope.
To get maximum performance from an oil-immersion type of objective with a numerical aperture of
1.0 or greater, an oil-immersion type of achromat/aplanat condenser must also be used. These con-
densers are used with oil applied between the specimen and the condenser lens.

Bubbles in the oil will adversely affect the viewing of the image, so be careful to prevent the forma-
tion of air bubbles. To check for air bubbles, remove the eyepieces, fully open the field and aperture
diaphragms, and look at the exit pupil of the objective within the eyepiece tube. (The exit pupil will
appear as a bright circle.) When it is difficult to see if there are any bubbles, mount a centering
telescope (sold separately) on the eyepiece sleeve with an adapter (sold separately). Then, while
turning the eyepiece on the centering telescope to change the focus, look through the centering
telescope for air bubbles. If there are bubbles in the oil, remove them by one of the following
methods:
» Turn the revolving nosepiece slightly, moving the oil-immersed objective back and forth once
or twice. (In the case of the condenser, gently turn the condenser focus knob to move the
condenser up and down slightly.)
* Add more oil.
* Remove the oil and replace it with new oil.

Use as little oil as possible (just enough to fill the space between the tip of the objective and the
specimen, or between the tip of the condenser and the specimen). If too much oil is applied, the
excess will flow onto the stage and around the condenser.

22
 3. Operation of Each Part

Any oil remaining on an oil-immersion type of objective or staining on the tip of a dry type of
objective has a negative effect on viewing. After using oil, wipe all of it away and make sure that
there is no oil on the tips of the other objectives. Also carefully wipe away the oil on the condenser
lens after use.

Use petroleum benzine to wipe away immersion oil. Removing the oil and wiping with absolute
alcohol (ethyl alcohol or methyl alcohol) will complete cleaning.
If you cannot obtain petroleum benzine, use methyl alcohol. Note that methyl alcohol does not
clean as well as petroleum benzine and it will be necessary to repeatedly wipe the surfaces (three or
four times is usually sufficient to clean the lenses.)

WARNING When using petroleum benzine or absolute alcohol, always follow the instructions
provided by the manufacturer. Keep these flammable liquids away from fire or
| sparks.

11 Water-immersion operation
(using a water-immersion type of objective and condenser)

An objective marked “WI” or “W” is a water-immersion type. These objectives are used with the
immersion water (distilled water or physiological saline) applied between the specimen and the tip
of the objective. Microscopy is performed in the same manner as that for an oil-immersion type of
objective.

Since water evaporates easily, periodically check it during observation. Applying excess water will
cause it to flow onto the stage and condenser and ultimately result in corrosion.

After use, wipe any water from the tip of the objective and condenser and complete the clean-up
process by wiping with absolute alcohol.
If the water dries and leaves a stain, apply a small amount of neutral detergent and wipe gently.
Complete the clean-up process by wiping with absolute alcohol.

23
 Assembly

WARNING To prevent electrical shock or fire, turn off the power switch (flip it to the “ ”
side) during assembly.

CAUTION «+ When assembling the microscope, assemble the eyepiece tube first.
+ When moving the microscope, be sure to detach the lamphouse and securely
grasp the arm from the back of the microscope with both hands.
* Do not grasp the coarse and fine focus knobs, eyepiece tube or stage, etc. when
carrying the microscope. This could cause the part to come off, resulting in
damage to the microscope.
+ Be careful not to pinch your hands or fingers when setting up the microscope.
* Viewing will be adversely affected if any of the lenses is scratched or has fin-
gerprints on it. Handle the lenses carefully during assembly.
* This microscope is a precision optical instrument. Handle it carefully and do
not subject it to a strong physical shock. (The accuracy of the objectives in
particular may be adversely affected by even a weak physical shock.)

Assemble each part in sequence as numbered in the diagram. (For details, refer to pages 25 to 29.)

Tools needed
Two hexagonal screwdrivers (provided)
When not using, place these in the tool holder in the back of the microscope.

Installation location
This product is a precision optical instrument, and using or storing it under unsuitable conditions
may damage it or may have an adverse effect on its accuracy. The following conditions should
be kept in mind when selecting the installation location.
* Avoid installing the microscope in a brightly lit location such as a room that receives direct
sunlight, or directly under room lights. The quality of the view through the microscope dete-
riorates if there is excessive ambient light.
¢ Install the microscope in a location that is free from dust or dirt.
* Install the microscope on a flat surface with little vibration.
* Install the microscope on a sturdy desk or table that is able to bear the weight of the instru-
ment.
* Do not install the microscope in a warm, humid location. In such a location, condensation or
mold may form on the lenses, degrading the performance or damaging the instrument.

24
 4. Assembly

[5]
Photomicrographic vertical
tube adapter

Clamp the photomicrographic

Eyepiece vertical tuber adapter
(3 screws) Tools
| 『 | . x2
Revolving E Trinocular Clamp the lamphouse
; iec tubi
nosepiece eyepiece tube Clamp the lamphouse cover
Clamp the
[4] | ^ eyepiece tube Lamphouse cover

= |

L Objective _ | ‘ 12V DC 100W

ーー 7 Clamp Clamp the <=> halogen lamp
Specimen ===" thé specimen revolving nosepiece
holder holder +
(2 screws)
Stage Lamphouse
Clamp
the stage ck
3
[a]
o E600 main unit
> [6]
Ρ Power con
d
ní

>
Condenser Clamp the substage
Substage
Clamp the condenser
(Left side)

1. Confirming the input voltage

Nikon
MODEL ECLIPSE E600
120V-
2.4A
50/60HZ
MADE iN JAPAN

WARNING Make sure that the input voltage indicated on the back panel of the microscope is
the same as the voltage provided in your area. If the indicated voltage is different,
do not use the microscope and notify your nearest Nikon representative immedi-
ately. If the microscope is used with the wrong input voltage, a short circuit,
electrical shock or fire may result, causing damage to the microscope.

25
 2. Lamp and lamphouse installation and removal (lamp replacement) EH

CAUTION + To prevent electrical shock and damage to the microscope, always turn off the
power switch (flip it to the “ :” side) and unplug the power cord when connect-
ing or disconnecting the lamphouse.
+ To prevent burns, allow the lamp and lamphouse to cool before replacing it for
at least 30 minutes after using.
* Use the Nikon C-LP HALOGEN 12V-100W model for the lamphouse.
* Use a 12 V-100 W LONGLIFE halogen lamp (OSRAM HLX 64623 or PHILIPS
7724).
* Do not touch the glass surface of the lamp with your bare hands. Doing so will
cause fingerprints, grease, etc., to burn onto the lamp surface, reducing the illu-
mination provided by the lamp. If you do get any fingerprints or dirt on the
lamp, wipe it clean.
* After replacing the lamp, make sure that the lamphouse cover is attached se-
curely. Never use the lamphouse while its cover is off.

Clamp lever

Check that the power switch is off (i.e., that it is flipped to the “ ” side).
N 一

If the lamphouse is mounted on the microscope, use a hexagonal screwdriver to loosen the
lamphouse clamp screw, and then remove the lamphouse from the microscope (the screw is
on the top of the lamphouse mount).
3 Use a hexagonal screwdriver to loosen the clamp screw on the front of the lamphouse and
remove the lamphouse cover.
4 While pressing the lamp clamp lever, push the lamp into the socket pin holes as far as it will
go. (Do not touch the glass portion of the lamp with your bare hands.)
5 Return the clamp lever to its original position. Make sure that the lamp is not tilted.
6 Attach the cover securely and tighten the clamp screw. Make sure that the cover is fixed in
place and will not come off even when shaken by hand.
7 Connect the lamphouse to the microscope by inserting the lamphouse plug into the lamphouse
socket.
8 Tighten the lamphouse clamp screw to secure the lamphouse in place.

26
 4. Assembly

3. Stage assembly MI

1 Substage installation
Use a hexagonal screwdriver to loosen the clamp screw on the right side of the substage.
Fit the substage over the substage mount on the microscope and slide it down as far as 1t will
go. Use a hexagonal screwdriver to tighten the substage clamp screw and secure the substage
in place.

2 Stage installation
Loosen the stage rotation clamp screw. Place the stage on top of the substage and fit it in
position so that it is level. Tighten the rotation clamp screw with the stage facing to the front.

Note on removing specimen holder

Release two clamp screws of the specimen holder with the stage dismounted from the sub-
stage. Securely clamp the screws when reinstalling the specimen holder.

3 Condenser installation
Turn the condenser focus knob to lower the condenser holder as far as it will go.
Insert the condenser in the condenser holder. Tighten the clamp screw on the left side with
the aperture scale on the condenser facing to the front.
Turn the condenser focus knob to raise the condenser as far as it will go.

| Observing a relatively thick specimen

Lower the substage according to the following procedure.
1 Turn the coarse focus knob to lower the substage as far as it will go and remove the stage.
2 Loosen the substage clamp screw with a hexagonal screwdriver and remove the substage.
3 Remove the upper limiting screw with a hexagonal screwdriver. (Store the screw in a safe
place to prevent its loss.)

Remove the upper

limiting screw.

4 Re-install the substage and stage in their original positions.

27
 4. Revolving nosepiece assembly L LgŽLg„LÉLg2K„2="Yx2„».—

1 Revolving nosepiece installation

Use a hexagonal screwdriver to loosen the revolving nosepiece clamp screw. Fit the revolv-
ing nosepiece into the revolving nosepiece mount on the microscope from the bottom and
slide it toward the rear of the microscope as far as it will go. Tighten the clamp screw to
secure the revolving nosepiece.

Note on removing the revolving nosepiece

First, remove all the objectives. Lower the stage completely, and hold the revolving nose-
piece in your hand so that it does not fall when you remove it.

2 Objective installation
Lower the stage completely. Screw the objective into the revolving nosepiece so that the
magnification increases when the nosepiece is rotated in the clockwise direction when look-
ing down on the nosepiece from above.

Note on removing objectives

If there is a specimen on the stage, remove it first. Lower the stage completely, and hold each
objective in both hands so that they do not fall when you remove them.

| 5. Eyepiece tube assembly MM

1 Eyepiece tube installation

Loosen the eyepiece tube clamp screw. Tilt and insert the round dovetail on the eyepiece tube
into the round dovetail mount on the microscope arm. Then tighten the eyepiece tube clamp
screw to secure the eyepiece tube in place.

2 Eyepiece installation
Install the left and right eyepieces, both with the same magnification.
There are positioning protrusions on the right sleeve. Align the notches of an eyepiece with
the protrusions on the sleeve and slide the eyepiece on. Some types of eyepiece tube do not
have such protrusions.
Fit the rubber eyeguard (sold separately) into the groove around the eyepiece.

28
 4. Assembly

| 6. Connecting the power cord o

WARNING Use only the following power supply cord set.

* For 100-120V area
UL Listed, detachable cord set, 3 conductor grounding type SVT, No. 18 AWG
rated at 125V, 7A minimum.
In case of using the extension cord, use only the power supply cord with the PE
(protective earth) wire.

* For 220- 240V area

3 pole power supply cord set, which must be approved according to EU/EN
standards.
Class I equipment should be connected to PE (protective earth) terminal.
In case of using the extension cord, use only the power supply cord with the PE
(protective earth) wire.

Make sure to turn off the microscope power switch (flip it to the “ `” side) before connecting the
power cord.
Plug the cord into the socket of the AC input connector on the back of the microscope. Securely
plug the other end of the cord into an AC outlet.

7. Installing separately sold accessories [SS

Install photomicrographic equipment and other separately sold accessories by referring to the in-
struction manual for each accessory.

29
 Troubleshooting Tables

Improper use of the microscope may adversely affect the performance even if it is not damaged. If any of
the problems listed in the table below occur, follow the countermeasures.

圖 Viewing and control systems

Problem Cause Countermeasure

The optical path selection lever on the

eyepiece tube is in an intermediate position.
Set the optical path selection lever to 100%
The optical path selection lever on the of the binocular eyepiece. (p. 15)
eyepiece tube is not set to 100% of the
binocular eyepiece.

The diffuser is in an intermediate position. Insert and remove correctly. (p. 20)

The revolving nosepiece has not been Install the revolving nosepiece correctly.
installed properly. (p. 28)

The revolving nosepiece has not been Turn the revolving nosepiece until it clicks
Vignetting or uneven rotated until it has clicked into place. into place. (Place the objective in the optical
brightness in the view field; (The objective is not in the optical path.) path.) (p. 11)
the entire view field cannot
be seen. Position the condenser so that the image of
The condenser is too low. the field diaphragm forms properly on the
specimen. (p. 17)

The condenser is not centered. Center the condenser. (p. 17)

The condenser is not installed properly. Install the condenser correctly. (p. 27)

The filters are not switched fully into Switch the filters correctly, (p. 20)
position.

The field diaphragm is stopped down too far. Open the diaphragm to a suitable size. (p. 19)

An unsuitable objective and condenser Use a suitable combination. (p. 19)

combination is being used.

The lamp is not installed properly. Install the lamp correctly. (p. 26)

Position the condenser so that the image of

The condenser is too low. the field diaphragm forms properly on the
specimen. (p. 17)

Dirt or dust in the view field. The aperture diaphragm is stopped down too Open the diaphragm to a suitable size. (p. 18)
far.

There is dirt or dust on the lens, condenser, Clean the components. (p. 34)
eyepiece, filter or specimen.

30
 5. Troubleshooting Tables

Problem Cause Countermeasure

The aperture diaphragm is stopped down too Open the diaphragm to a suitable size. (p. 18)
far.

Position the condenser so that the image of

The condenser is too low. the field diaphragm forms properly on the
specimen. (p. 17)

The cover glass is too thick. Use the specified type of cover glass
(thickness: 0.17 mm).
There is no cover glass.

There is no oil on the tip of an oil-immersion

Viewing is poor (too much or type of objective.
Apply Nikon immersion oil. (p. 22)
too little contrast, poor
resolution). The specified immersion oil is not being
used.

There are bubbles in the immersion oil. Remove the bubbles. (p. 22)

There is immersion oil on the tip of a dry Clean the components. (p. 23)
type of objective.

The compensation ring on an objective fitted

Adjust the compensation ring according to
with a compensation ring has not been
the cover glass.
adjusted.

There is dirt or dust on the lens, condenser,

Clean the components. (p. 34)
objective or specimen.

The revolving nosepiece has not been Install the revolving nosepiece correctly.
installed properly. (p. 28)

The revolving nosepiece has not been Turn the revolving nosepiece until it clicks
rotated until it clicks into place. into place. (p. 11)
Uneven focus.

The specimen is not secured in place on the Install the specimen property in the
stage. specimen holder on the stage.

The stage has been installed slanted. Install the stage correctly. (p. 27)

The revolving nosepiece has not been Install the revolving nosepiece correctly.
installed properly. (p. 28)

The revolving nosepiece has not been Turn the revolving nosepiece until it clicks
rotated until it clicks into place. into place. (p. 11)

image flows.
The specimen is not secured in place on the Install the specimen properly in the
stage. specimen holder on the stage.

The condenser is not centered. Center the condenser. (p. 17)

The stage has been installed slanted. Install the stage correctly. (p. 27)

31
 Problem Cause Countermeasure

An NCB11 filter is not being used. Use the NCB11 filter. (p. 20)
The image is yellowish.
The lamp voltage is too low.
Push auto-photo switch and then adjust the
The image is too bright. The lamp voltage is too high. brightness through the ND filter combina-
tion. (p. 20 and 21)

The lamp voltage is too low.

Inadequate illumination The aperture diaphragm is stopped down too Open the diaphragm to a suitable size. (p. 18)
(also check the electrical far.
system problems and
countermeasures). Position the condenser so that the image of
The condenser is too low. the field diaphragm forms properly on the
specimen. (p. 17)

Set the specimen on the stage with the cover

The specimen is placed upside-down. glass facing up.
Focusing is not possible with
high-power objectives.
Use the specified type of cover glass
The cover glass is too thick. (thickness: 0.17 mm).

Set the specimen on the stage with the cover

The specimen is placed upside-down. glass facing up.
The objective strikes the
specimen when changing from
Use the specified type of cover glass
a low-power objective The cover glass is too thick. (thickness: 0.17 mm).
to a high-power objective.

The diopter setting has not been adjusted. Adjust the diopter setting. (p. 16)

The difference in focus is large

when the objective is changed. The diopter setting has not been adjusted. Adjust the diopter setting. (p. 16)

The specimen does not move The specimen holder is not fixed securely in Secure the specimen holder in place. (p. 27)
smoothly. place on the stage.

When viewing through The interpupillary distance has not been Adjust the interpupillary distance. (p. 16)
the binocular eyepiece, adjusted.
the image does not resolve
into a single image. The diopter setting has not been adjusted. Adjust the diopter setting. (p. 16)

The interpupillary distance has not been Adjust the interpupillary distance. (p. 16)
adjusted.

Eye strain develops while

viewing. The diopter setting has not been adjusted. Adjust the diopter setting. (p. 16)

Adjust the brightness through the ND filter

The brightness level is not suitable.
combination. (p. 20)

32
 5. Troubleshooting Tables

2 Electrical system

Problem Cause Countermeasure

The power is not being supplied. Plug the power cord into an outlet. (p.29)

The power cord is not connected to the

microscope. Connect t th the power cord . (p. . 29)

The lamp does not light when

the power switch is turned on. The lamp has not been installed. Install the lamp. (p. 26)

The lamp is burned out. Replace the lamp. (p. 26)

Use the specified lamp (refer to the electrical

The specified lamp is not being used.
specifications on p. 35). (p. 26)

The lamp is near the end of its life. Replace the lamp. (p. 26)

The power cord is not connected securely. Secure the connection. (p. 29)
The lamp flickers;
the brightness is unstable. The lamp is not plugged into its socket Insert the lamp securely into its socket.
securely. (p. 26)

The lamphouse is not connected to the

microscope securely. Connect the lamphouse securely. (p. 26)

33
 Care and Maintenance

Lens cleaning

Do not let dust, fingerprints, etc. get on the lenses. Dirt on the lenses, filters, etc. will adversely
affect the view of the image. If any of the lenses gets dirty, clean it as described below.

* Either brush away dust with a soft brush, or gently wipe away with gauze.
* To remove fingerprints or grease, use a piece of soft, clean cotton cloth, lens tissue, or gauze
moistened with absolute alcohol (ethyl alcohol or methyl alcohol).
* Use petroleum benzine to clean off immersion oil (p. 23).
* Do not wipe the entrance lens on the eyepiece tube with petroleum benzine.
« Absolute alcohol and petroleum benzine are both highly flammable. Be careful when handling
them, when around open flames, or when turning the power switch on and off.
* Follow the instructions provided by the manufacturer when using absolute alcohol and petroleum
benzine.

| 2 Cleaning painted components

Do not use organic solvents such as alcohol, ether or paint thinner on painted components, plastic
components or printed components. Doing so could result in discoloration or in peeling of the
printed characters. For persistent dirt, dampen a piece of gauze with neutral detergent and wipe
lightly.

3 | Storage

Store the microscope in a dry place where mold is not likely to form.
Store the objectives and eyepieces in a desiccator or similar container with a drying agent.
Put the vinyl cover over the microscope after use to protect it from dust.
Before putting on the vinyl cover, turn off the microscope power (flip the switch to the“ ” position),
and wait until the lamphouse has cooled.

4 Regular inspection

Regular inspection of this microscope is recommended to maintain peak performance. Contact your
nearest Nikon representative for details about regular inspection.

34
Electrical Specifications

Ml ECLIPSE E600 (Main body of the microscope)

Input Power Supply Voltage: + AC 100/120V + 10% 50/60Hz
Current consumption: 2.4A or less
Internal fuse rating: 125V, SA
* AC 230V + 10% 50/60Hz
Current consumption: 1.2A or less
Internal fuse rating: 250V, T3.15A
Lamp Rating: 12V DC, 100W halogen lamp
(OSRAM HLX 64623 or PHILIPS 7724)
Protection Class: Class I

Operating Environmental * Temperature: 0 to +40°C

Conditions: * Humidity: 85% RH max., non-condensing
* Altitude: 2000 m max.
* Pollution: Degree 2
* Installation: Category II
* Indoor use only
Conforming Standards
e Product with AC 120V + UL Listed product
* FCC 15B Class A satisfied
This equipment has been tested and found to comply with the limits
for a Class A digital device pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protections against
harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and if not installed and used in accordance with
the instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area
is likely to cause harmful interference in which the user will be
required to correct the interference at this own expense.

This Class A digital apparatus meets all requirements of the Canadian

Interference-Causing Equipment Regulations.
Cet appareil numérique de la Classe A respecte toutes les exigences
du Règlement sur le matériel brouilleur du Canada.
+ Product with AC 230V + GS approved product
* EU Low Voltage Directive satisfied
+ EU EMC Directive satisfied

35
 Index

Absolute alcohol 23, 24

Aperture diaphragm 18
Aperture diaphragm ring 18
Auto-photo switch 21
Centering telescope 18
Coarse and fine focus knobs 13
Coarse focus stopper 14
Color balancing filter (NCB11) 20, 21
Color compensation (CC) filter 21
Color film 21
Color temperature 21
Condenser focusing and centering 17
Contrast 18
Diopter adjustment 16
Diopter adjustment ring 16
Eyepiece tube lever clicking 15
Field diaphragm 19
Input voltage 25
Interpupillary distance adjustment 16
Lamp voltage 21
Numerical aperture 18
Oil-immersion 22, 23
Installation location 24
Petroleum benzine 23, 34
Photo vertical tube adapter 15
Power cord 29
Power switch 24
Round dovetail 29
Specified lamp 26
Specimen holder 27
Substage 27
Trinocular eyepiece tube 10
Trimming 21
Torque adjustment 13
TV vertical tube adapter 15
Water-immersion 23
Wiping off oil 23

36