E100 User
E100 User
E100 User
Microscope
ECLIPSE E100
Instructions
06.10.NF.1
Thank you for purchasing this Nikon product. This instruction manual is for the users of the
Nikon Microscope ECLIPSE E100 describing basic operation of the microscope. To ensure
correct usage, please read this manual thoroughly before using the microscope.
It is prohibited to reproduce or transmit this manual in any form without the prior
consent of Nikon.
The instructions and specifications in this manual are subject to change without
notice.
Although every effort has been made to ensure the accuracy of this manual, if
you find that any part of this manual is unclear or incorrect, contact your nearest
Nikon representative.
Some ECLIPSE E100 microscope sets may come with different components
and accessories from those shown in this manual.
Also read the manuals for the products used with the microscope, for example,
the Nikon photomicrographic equipment.
Meaning
WARNING
CAUTION
Meaning
Caution! Hot surface
This symbol label attached near the field lens unit (the lamp is set
underneath the field lens unit) and on the bottom surface of the base plate
(the lamp is set above this place) reminds you of the following.
WARNING: Risk of burn injury. The lamp and its surroundings (including
the field lens unit) become hot during use. Do not touch them
while the lamp is on and for thirty minutes after the lamp is
turned off.
CAUTION: The lamp and its surroundings remain hot even after the lamp
is turned off. Make sure that they are sufficiently cool before
replacing the lamp.
Caution!
This symbol label attached near the AC inlet reminds you of the following.
CAUTION: Make sure that the input voltage set by the voltage selector
matches the voltage provided in your region.
The voltage selector on the microscope sent to the United
States is set to 120V at the factory.
1. Purpose
WARNING
WARNING
There are no user serviceable parts inside the microscope. Disassembly will void the warranty, and
could degrade the performance, cause electrical shock or personal injury, or damage the instrument.
If you have a service problem, contact your nearest Nikon representative.
WARNING
The input voltage is indicated in two places at the rear of the microscope: on the nameplate and above
the AC-IN socket. Confirm that these input voltage indications correspond to the voltage provided in your
region. If not, follow one of the instructions below. The use of microscopes with the different input
voltage indications will cause overcurrent and overheating, which may result in fire or severe damage to
the microscope.
If the voltage indication on the nameplate differs:
Do not plug in the microscope. Contact your nearest Nikon
representative.
If the voltage indication above the AC-IN socket differs:
Refer to p. 26 and change the input voltage setting before
turning on the power switch.
For the microscope with the nameplate showing
Input Voltage
Indications
[100/110/120 V ]:
The voltage can be set to: AC 100 V, 110 V or 120 V.
For the microscope with the nameplate showing
[220/230/240 V ]:
The voltage can be set to: AC 220 V, 230 V or 240 V.
WARNING
Use the specified lamp and fuse. For the microscope with the nameplate showing [100/110/120 V], use
the power cord provided. For the microscope with the nameplate showing [220/230/240 V], use the
specified power cord. Using an incorrect lamp, fuse, or power cord may damage the instrument or cause
a fire. (Also see p. 41-42 on power cord.)
Specified Lamp
Halogen lamp 6V-20W (PHILIPS 7388 or OSRAM HLX64250)
Specified Fuse
For the microscope with the nameplate showing [100/110/120 V ]:
250 V, 1 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)
For the microscope with the nameplate showing [220/230/240 V ]:
250 V, 0.5 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)
WARNING
Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire.
Also turn off the power switch and then unplug the power cord before assembling the microscope, and
before changing the lamp or fuse. To turn off the power, turn the power switch to .
CAUTION
Keep the microscope free of moisture to prevent short circuiting that could result in overheating or other
malfunctions. If water splashes on the microscope, immediately turn off the power switch (turn the switch
to ) and unplug the power cord. Then, wipe off the water with a dry cloth. Short circuiting can also
result when foreign matter is trapped inside the microscope. If foreign matter or water has entered the
microscope, do not use the microscope and contact your nearest Nikon representative.
CAUTION
The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be sure
the lamp has been off for 30 minutes before touching it.
When changing the lamp bulb, make sure that the lamp is cool enough to touch (the light should be
off at least 30 minutes).
Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so could
result in burns.
Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine,
acetone, thinner, or alcohol near a hot lamp, as a fire could result.
8. Stage Rack
CAUTION
The rack for the stage protrudes during use. Be careful not to hit the rack with your hand or other parts of
your body when handling the microscope as you may get hurt by an edge of the rack.
Rack
9. Installation
This microscope is a precision instrument. Using the microscope in an unfavorable environment could
result in malfunctions or degraded performance. Consider the following conditions when choosing the
installation location.
Observation conditions are better if light from windows and bright room light can be avoided.
Install the microscope in a location with a room temperature of 0 to 40 C and with a maximum
relative humidity of 85%. High temperature and humidity are to be avoided because they promote
mold growth and condensation, which may damage the microscope.
Dirt and dust degrade optical performance and are to be avoided.
Vibrations in the environment will degrade the image. Install the microscope in a location free of
vibrations.
Install the microscope on a solid table and keep the microscope level.
This microscope emits a feeble electromagnetic wave. Do not place a precision electronic device
near the microscope as precision could be degraded. Also, avoid placing a radio or TV near the
microscope as reception of sound and images may be hampered.
Install the microscope in the location of 10 cm or more away from the surrounding walls.
Install the microscope in the place that allows easy detachment of the power cord from the AC inlet
in the event of emergency.
WARNING
When using petroleum benzine or absolute alcohol to wipe off immersion oil or
to clean the lenses, follow the instructions provided by their manufacturers.
Absolute alcohol and petroleum benzine are inflammable. Take great care
when handling them.
Shipping Clamps
Contents
II.
Miscellaneous Operations 22
1. Oil-Immersion Observation 22
2. Adjusting the Torque of the Coarse Focus Knob 24
3. Photomicrography and TV Microscopy 24
4. Cord Hangers 24
VI. Assembly 25
1. Assembly of Standard Set26
2. Assembly of Additional Components28
1) Condenser 28
2) Objectives 29
3) Specimen Holder 29
4) Eyepiece and Eyepiece Pointer 29
5) Monocular eyepiece tube 29
6) Other Accessories 29
3. Replacement of Consumable Materials 30
1) Replacing the Lamp 30
2) Replacing the Fuse 31
1 Basic unit
2 Eyepieces
Screwed on to the eyepiece tube.
3 Eyepiece tube
This is a binocular eyepiece tube. A trinocular eyepiece tube is available for
photomicrography and TV microscopy. Amonocular eyepiece tube can be used also.
4 Objectives
Objectives with various magnifying powers are available.
5 Condenser
Used for condensing light. The condenser should be positioned slightly lower
than its upper limit.
Adjust the aperture diaphragm lever according to the objective.
6 Field lens unit
Pull the field lens unit upward when changing lamp.
7 Lamp
Halogen lamp of 6 V-20 W is used.
8 Fuse
For the microscope with the nameplate showing [100/110/120 V], 250 V, 1 A, time-lag lowbreaking type, 5X20 miniature fuses (two pieces) are used.
For the microscope with the nameplate showing [220/230/240 V], 250 V, 0.5 A, time-lag lowbreaking type, 5X20 miniature fuses (two pieces) are used.
9 Power cord
Use the power cord provided.
2 Eyepieces
3 Eyepiece tube
1 Basic unit
4 Objectives
8 Fuse (x2)
5 Condenser
9 Power cord
7 Lamp
10
1 Diopter ring
7 Stage
2 Revolving nosepiece
3 Specimen holder
8 Power switch
5 Condenser aperture
diaphragm lever
18 Voltage selector
(Fuse holder)
19 Nameplate
15 Coarse focus
knob torque
adjustment ring
20 AC-IN socket
16 Coarse focus knob
17 Fine focus knob
11
Match the
bottom edge
of the diopter
ring with the
engraved
base line.
Viewfields
12
And focus
with this ring.
Observe.
Then focus
with this ring.
13
Lamp Illumination
Turn on the power switch (turn to |) and the
lamp will come on. Turn the brightness control
dial to adjust the brightness of the viewfield.
(Turning the dial clockwise increases the
brightness; turning the dial counterclockwise
decreases it.)
Power ON
Darkens.
2)
Brightens.
Interpupillary Distance
Adjustment
Adjust the distance between the eyepieces to
merge the right and left viewfields into one.
(This is an adjustment to match the distance
between eyepieces with the distance between
your eyes).
3)
14
4)
Specimen Mounting
Place specimen slide on the stage with the
coverglass facing upward. Open the claw of
the specimen holder with your finger at the root
tilt and fix the specimen slide with the claw.
Coverglass
5)
15
Focusing
Turning the focus knobs recklessly is a long and hard way to focus on the image. If you are
using a high power objective, you may even damage the specimen by pressing it against the
objective. Before breaking the coverglasses or damaging the objectives, read the following
and find the correct way to focus on the specimen.
(1) Put the 10x (or 4x) objective in the optical path.
(2) Turn the coarse focus knob to raise the stage to its upper limit. * 1
(3) Looking into the eyepieces, slowly rotate the coarse focus knob to lower the
stage. When the specimen image appears, stop rotating the knob.
(4) Rotate the fine focus knob and precisely focus on the image.
When you want to observe the image with a high power objective, first focus on
the image using a 10x (or 4x) objective. Then change to a high power objective
and rotate the fine focus knob for precise focusing.
1. When rotating the coarse focus knob while looking into the eyepieces, be sure to
turn it only in the direction that lowers the stage.
2. When raising the stage using the coarse focus knob, take your eyes off the
eyepieces and look at the gap between the upper surface of the specimen and the
front of the objective from the side.
3. First focus with a low power objective. Then change to a high power objective.
*1: Since the working distances of 10x and 4x objectives are large (p. 33), these objectives do not touch
the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses
of a standard thickness are used. (The standard thickness for slides is 1.2 mm and that for coverglass
is 0.17 mm.)
16
Method 2:
Swing the 40x objective into the optical path. While looking at the microscope from the side,
rotate the coarse focus knob until the specimen almost touches the objective (about 0.5 mm
apart from the front of the objective). The specimen is now almost in focus. Switch to the 10x
objective, look into the eyepieces, and rotate the fine focus knob slightly to find the focal point.
Be careful not to hit the objective with the specimen.
17
6)
18
7)
8)
Objective Selection
Rotate the revolving nosepiece to the desired objective magnification. (The objective will click into
place when rotated into position.)
Adjust the aperture diaphragm lever according to the selected objective (see the procedure 9)).
9)
60
40
20
10
19
7080%
100%
Objective pupil
Aperture diaphragm image
The appropriate size of the aperture diaphragm
20
40
20
10
21
Miscellaneous Operations
Oil-Immersion Observation
1
The Oil mark on the side of an objective indicates that it is an oil-immersion type objective. (The
oil-immersion objective also has a black band around the barrel end.) An oil-immersion objective
is used with the immersion oil applied between the front of the objective and the coverglass. For
an oil-immersion objective with a numerical aperture of 1.0 or more, use of an oil-immersion type
condenser is required to take full advantage of its performance. An oil-immersion type condenser,
like an oil-immersion type objective, needs immersion oil to be applied between the front of the
condenser and the coverglass.
The abbe condenser included in the bright viewfield set can be used for oil immersion observation.
Example of Oil-Immersion
Condenser:
Move the specimen toward the back and
lower the condenser slightly. Add a drop
of oil on the front of the condenser from
the long hole on the stage. Bring the
specimen back over the condenser and
slowly raise the condenser.
Objective:
Rotate the revolving nosepiece to move
the objective out of position. Add a drop
of oil to the specimen. Slowly rotate the
revolving nosepiece to bring the objective
back into position.
22
WARNING
When handling petroleum benzine and absolute alcohol, be sure to follow the instructions
provided by the manufacturers. Since they are highly inflammable take great care when
handling them.
23
Loosens.
Tightens.
Cord Hangers
3
Push the cord hangers (optional) into the holes
on the rear of the microscope. The hangers
can be used for winding the power cord
around when the microscope is not in use.
To remove the hangers, use a screwdriver.
24
Cord hangers
Assembly
Read the Common Sense Safety Instructions in this manual before assembling the microscope.
Be sure to follow the instructions written therein. Also, make sure that the power switch is off
(turned to ) before assembly to prevent electrical shock.
25
Lock plate
Fuses
Voltage selector
Window
Voltage
indication
Fuse holder
(3) Wear gloves and remove two fuses and the voltage
selector from the fuse holder.
(4) Attach the voltage selector to the fuse holder so that the indication of the voltage
provided in your region appears in the window of the fuse holder.
(5) Put the fuses and the fuse holder back in place. Be sure to push both sides of the fuse
holder firmly till it clicks into place.
26
CAUTION
When detaching the eyepiece tube, hold it firmly with your hand to prevent it from
falling and loosen the clamp screw.
27
28
Blue filter
Filter holder
2) Objectives
Objectives are attached to the microscope before shipment.
When replacing an objective, remove the specimen from the stage and lower the stage.
Remove the objective holding it with both hands. Be careful not to drop the objective.
Screw a new objective into the revolving nosepiece. Set the objectives in such an order
that the objective magnification increases as the revolving nosepiece is turned clockwise
(as viewed from the top of the microscope).
3) Specimen Holder
The specimen holder is attached to the stage before shipment.
When removing the holder, loosen the two hexagonal socket head bolts furthermost from the
specimen using the hexagonal wrench provided.
Eyepiece pointer
Eyepiece
5) Other Accessories
For installation of other accessories such as the photomicrographic equipment, see the
manual provided for each product.
29
CAUTION
The lamp is hot when in use. To avoid burn injury, turn off the microscope and
wait at least 30 minutes (until the lamp is cool enough to touch) before
attempting to change the lamp.
Make sure that the contacts of the lamp and socket are not damaged before
installing a new lamp. If the contacts are damaged, they may cause poor
illumination or overheating.
Insert the lamps contact pins fully into the socket holes. If the pins are loose,
the lamp could come loose or result in a contact failure, which will cause
overheating or smoke.
Be sure to put the field lens unit back in place after replacing the lamp. Never
turn on the lamp without the field lens unit.
Do not touch the glass part of the lamp using your bare hands. Wear gloves or
use a cloth when handling the lamp to protect the surface from fingerprints.
Wipe off any fingerprints or stains using a clean cloth moistened with alcohol.
Fingerprints will etch into the hot surface of the lamp and reduce the brightness,
damage the lamp or reduce its service life.
Handle the lamp gently. Shocks and vibrations will damage the lamp or reduce
its service life.
30
(1) Turn off the power switch (turn to ) and unplug the power cord.
(2) Wait about 30 minutes until the lamp and its surroundings are cool enough to touch.
(3) Hold the field lens unit at the dents on both sides and pull it upward slowly while slightly
moving laterally to remove it.
(4) Remove the old lamp.
(5) Hold a new lamp wearing gloves or using a cloth. Insert the lamps contact pins fully
into the socket holes straight and securely.
(6) Put the field lens unit back in place.
(7) Plug the power cord.
Socket
Lamp
31
CAUTION
Make sure that the contact of the fuse is not damaged before installing a new
fuse. If the contact is damaged, a malfunction or overheating may result.
Attach the fuse to the fuse holder securely. If not, the fuse may come loose or a
contact failure may occur, resulting in overheating or smoke.
Put back the fuse holder securely to its original position.
(1) Turn off the power switch (turn to ) and unplug the power cord.
(2) Remove the fuse holder using the flatblade precision screwdriver. (Use the tip of the
screwdriver to push the two lock plates toward the center of the fuse holder. The fuse
folder pops out from the AC -IN socket.)
(3) Wear gloves and emove two fuses and the
voltage selector from the fuse holder.
(4) Attach the voltage selector to the fuse holder
so that the indication of the voltage provided
in your region appears in the window of the
fuse holder.
(5) Put the fuses and the fuse holder back in
place. Be sure to push both sides of the
fuse holder firmly till it clicks into place.
Lock plate
Fuses
Voltage selector
Window
Voltage
indication
32
Fuse holder
Optical Characteristics
Combinations of 10x (Field No. 18) Eyepiece with
Objectives
1
Objective
Total
Numerical
Magnification Magnification Aperture
Real
Viewfield
Depth of
Focus
Resolving
Power
Working
Distance
40
0.10
4.5 mm
63.2 m
2.8 m
25 mm
10
100
0.25
1.8 mm
10.1 m
1.1 m
5.6 mm
40
400
0.65
0.45 mm
1.2 m
0.4 m
0.6 mm
60
600
0.80
0.3 mm
0.7 m
0.3 m
0.24 mm
100
1000
1.25
0.18 mm
0.4 m
0.2 m
0.14 mm
Microscope Terminology
2
(1) Total Magnification
The total magnification of a microscope is the individual magnifying power of the objective
multiplied by that of the eyepiece.
(2) Numerical aperture (N.A.)
The numerical aperture is an important factor in determining the efficiency of the condenser
and objective. It is represented by the formula:
N.A. = n sin
where n is the refractive index of the medium (air, immersion oil, etc.) between the
objectivelens and the specimen or condenser, and is half of the maximum angle at which
light enters or leaves the lens from or to a focused object point on the optical axis.
The larger the numerical aperture the brighter the image and the higher the resolution.
(3) Resolving Power
The ability of an optical system to discriminate between two discrete objects separated by a
minute distance. The more minute the distance, the higher the resolving power of the optical
system. In relation to the numerical aperture, the resolving power is represented by the
following formula:
Resolving power =
2N.A.
where is the used wavelength of light. (The resolving power in the above table is indicated
for = 0.55m.)
(4) Working Distance (W.D.)
The clearance between the front of the objective and the upper surface of the coverglass,
when a specimen image is sharply focused. Generally, the higher the magnifying power of the
objective, the shorter the working distance.
33
n
n
1000
7MN.A.
2N.A.2
34
Troubleshooting Tables
If difficulties should be encountered in the course of operation, please recheck the symptoms,
referring to the tables below, before contacting your nearest Nikon representative.
Optical
1
Troubles
Darkness at the periphery,
no viewfield seen, or
uneven viewfield
brightness.
Causes
Corrective Measures
35
Troubles
Poor image quality (low
resolution, contrast too
low or too high)
Causes
Corrective Measures
Immersion oil found on dry type Clean the objective. (P. 40)
objective (especially 40x and
60x objective).
Aperture diaphragm opened or Close or open properly.
closed too far.
(P. 19, 20)
Correction ring on the objective Adust the ring according to the
cover glass thickness.
not adjusted (only for the
objective with the correction
ring).
Dust or dirt on the eyepieces,
objective or condenser.
No-cover-glass-objective is
Use the ordinary obective for
used for the specimen with the cover glassed specimen.
cover glass.
The ordinary objective is used
for the specimen without the
cover glass.
36
Troubles
Image dark on one side.
Causes
Corrective Measures
click-stop position.
Insufficient brightness.
(Also see the causes and corrective measures for electrical problems.)
37
Mechanical Problems
2
Troubles
Causes
High-power objective
contacts slide when
changed over from low
power.
Note:
Inadequate brightness or
illumination.
38
Corrective Measures
Electrical Problems
3
Troubles
Causes
Flickering or unstable
lamp brightness.
Corrective Measures
Check power cord connection.
(P. 28)
39
Periodical Inspections
4
To maintain the performance of the microscope, periodical inspections and maintenance are
recommended.
For details, contact your nearest Nikon representative.
40
Specifications
(1) Model Name:
(4) Stage:
Stroke:
2 m/graduation
0.2 mm up or down / revolution
about 37.7 mm up or down / revolution
2 mm upward and 20 mm downward from the
focal plane.
X axis: 78 mm
Y axis: 40 mm
Output Rating:
6V 3.3A max.
Input Rating:
Frequency:
50 - 60 Hz
Voltage fluctuation:
10%
Rated current:
0.5 A
Fuse rating:
Power Cord:
41
Frequency:
50 - 60 Hz
Voltage fluctuation:
10%
Rated current:
0.3 A
Fuse rating:
Power Cord:
Protection Class:
Class I
0 to 40C
Relative Humidity:
Altitude:
2000m Max.
Pollution:
Degree 2
Installation Category
(Overvoltage Category): Category 2
For indoor use only.
(8) Storage Environment
Room Temperature:
-20 to 60C
Relative Humidity:
(The model for 100, 110, and 120 V areas is not covered by the FCC.)
42