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    994172_cellGrade_proliferation

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    Lesli Melani Lima Choccelahua
    The document details an experiment on the proliferation of HepG2 cells on BRAND plates® cellGrade™ plus surface. It describes the culture conditions, metabolic activity measurement using resazurin, and the results indicating higher cell numbers on treated plates compared to non-treated ones. The conclusion highlights that the BRAND plates® with cellGrade™ plus surface effectively support HepG2 cell attachment and proliferation.

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    994172_cellGrade_proliferation

    Uploaded by

    Lesli Melani Lima Choccelahua
    1 views1 page
    The document details an experiment on the proliferation of HepG2 cells on BRAND plates® cellGrade™ plus surface. It describes the culture conditions, metabolic activity measurement using resazurin, and the results indicating higher cell numbers on treated plates compared to non-treated ones. The conclusion highlights that the BRAND plates® with cellGrade™ plus surface effectively support HepG2 cell attachment and proliferation.

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    The document details an experiment on the proliferation of HepG2 cells on BRAND plates® cellGrade™ plus surface. It describes the culture conditions, metabolic activity measurement using resazurin, and the results indicating higher cell numbers on treated plates compared to non-treated ones. The conclusion highlights that the BRAND plates® with cellGrade™ plus surface effectively support HepG2 cell attachment and proliferation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    1 views1 page

    994172_cellGrade_proliferation

    Uploaded by

    Lesli Melani Lima Choccelahua
    The document details an experiment on the proliferation of HepG2 cells on BRAND plates® cellGrade™ plus surface. It describes the culture conditions, metabolic activity measurement using resazurin, and the results indicating higher cell numbers on treated plates compared to non-treated ones. The conclusion highlights that the BRAND plates® with cellGrade™ plus surface effectively support HepG2 cell attachment and proliferation.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Technical Note

    Proliferation of HepG2 cells on


    BRAND plates ® cellGrade ™ plus surface
    800

    PS
    Culture conditions A 700
    cellGrade™
    For each experiment HepG2 cells were 600
    seeded at a density of 6000 cells/cm2

    relative fluorescence units


    in wells of transparent 96-well F-bottom 500
    BRANDplates® and
    cultivated in DMEM 400
    medium containing
    7 % FCS at 37° C,
    300
    95% relative humi-
    dity and 5 % CO2.
    200

    100

    0
    1DIV 2DIV 3DIV

    B
    PS
    cellGrade™ plus

    1DIV 2DIV 3DIV

    A Metabolic activity measured by resazurin-resafurin turn over is used for rela-


    tive quantification of cell numbers after 2 and 3 days post seeding. HepG2 cells
    were incubated in presence of 50 µM resazurin for 3 hours prior to fluorescence
    measurement (Ex 506 nm/Em 635 nm) in a plate reader (GeminiEM Molecular
    Devices). HepG2 cells cultivated on BRANDplates® cellGrade™ plus show higher
    fluorescence signals indicating higher cell numbers after 2 and 3 days in vitro (DIV)
    when compared to non-treated microplates (PS). Resafurin fluorescence measured
    in cell-free wells was used for background correction. Data represent mean and
    standard deviation of 8 measurements.
    9941 72 · Printed in Germany · WA/0517

    B Representative images of HepG2 cells cultivated on non treated (PS) and


    cellGrade™ plus treated microplates at corresponding time points (200 x magni-
    fication).

    Conclusion
    BRANDplates® with cellGrade™ plus surface perfectly support attachment
    and proliferation of HepG2 cells.

    BRAND GMBH + CO KG · P.O. Box 11 55 · 97861 Wertheim · info@brand.de · www.brand.de May 2017

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