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BIOC 0113(1)

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BIOC 0113

CHUKA UNIVERSITY

UNIVERSITY EXAMINATIONS
EXAMINATION FOR THE AWARD OF DIPLOMA IN ANIMAL HEALTH AND
PRODUCTION
BIOC 0113: BASIC BIOCHEMISTRY

STREAMS: DIP. ANHE TIME: 2 HOURS

DAY/DATE: MONDAY 15/4/2019 11.30 A.M. – 1.30 P.M.


INSTRUCTIONS:
 Answer question ONE and any other TWO questions
 Do not write on the question paper

QUESTION ONE
a) Define the following terms as used in Biochemistry and state their significance (3 marks)
i. Metabolism
ii. Enzyme
b) The foods of ruminants, forages and fibrous roughages, consist mainly of β -linked

polysaccharides such as cellulose, which cannot be broken down by mammalian digestive


enzymes. Briefly describe the general digestion of food in ruminants. (9 marks)
c) Differentiate between the following: (4 marks)
i. Anabolism and catabolism
ii. Exergonic and endergonic reactions
d) Outline 6 biological functions of lipids (3 marks)
e) Differentiate between a nucleoside and a nucleotide and give an example of each.
(2 marks)
f) Describe the fates of pyruvate. (6 marks)
g) Outline the various functions of proteins. (3 marks)

QUESTION TWO
a) Using structural formulae, describe the reactions in glycolysis. (10 marks)
b) Describe how enzymes catalyze chemical reactions. (10 marks)

QUESTION THREE

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BIOC 0113

a) Using structural formulae, describe the basic steps of β -oxidation pathway of

saturated fatty acids. (7


marks)
b)
i. Define the digestibility of a food as used in animal nutrition. (1 mark)
ii. Discuss (6) factors affecting digestibility of a food in ruminants and non-ruminant
animals. (12 marks)

QUESTION FOUR
a) Draw the structure of ATP and explain how it is utilized in energy coupling links reactions
in the cell. (10 marks)
b) Enzymes are essentially proteins with large molecular weight. Using hexokinase gene as
an example, describe gene expression. (10 marks)

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