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    LB Agar Specifications 1

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    Chinna Kaniganti
    Luria Bertani Agar Miller is a nutrient-rich growth medium used for cultivating and maintaining recombinant strains of Escherichia coli for genetic and molecular studies. It contains casein enzymic hydrolysate, yeast extract, and sodium chloride to provide peptides, vitamins, sodium ions, and maintain osmotic equilibrium. The medium is prepared by suspending the powder in water, boiling to dissolve, then autoclaving and pouring into plates. Quality control ensures the medium supports luxuriant growth of three E. coli strains.

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    LB Agar Specifications 1

    Uploaded by

    Chinna Kaniganti
    28 views1 page
    Luria Bertani Agar Miller is a nutrient-rich growth medium used for cultivating and maintaining recombinant strains of Escherichia coli for genetic and molecular studies. It contains casein enzymic hydrolysate, yeast extract, and sodium chloride to provide peptides, vitamins, sodium ions, and maintain osmotic equilibrium. The medium is prepared by suspending the powder in water, boiling to dissolve, then autoclaving and pouring into plates. Quality control ensures the medium supports luxuriant growth of three E. coli strains.

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    Luria Bertani Agar Miller is a nutrient-rich growth medium used for cultivating and maintaining recombinant strains of Escherichia coli for genetic and molecular studies. It contains casein enzymic hydrolysate, yeast extract, and sodium chloride to provide peptides, vitamins, sodium ions, and maintain osmotic equilibrium. The medium is prepared by suspending the powder in water, boiling to dissolve, then autoclaving and pouring into plates. Quality control ensures the medium supports luxuriant growth of three E. coli strains.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    28 views1 page

    LB Agar Specifications 1

    Uploaded by

    Chinna Kaniganti
    Luria Bertani Agar Miller is a nutrient-rich growth medium used for cultivating and maintaining recombinant strains of Escherichia coli for genetic and molecular studies. It contains casein enzymic hydrolysate, yeast extract, and sodium chloride to provide peptides, vitamins, sodium ions, and maintain osmotic equilibrium. The medium is prepared by suspending the powder in water, boiling to dissolve, then autoclaving and pouring into plates. Quality control ensures the medium supports luxuriant growth of three E. coli strains.

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    HiMedia Laboratories

    Technical Data

    Luria Bertani Agar Miller

    M1151

    Luria Bertani Agar Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms. Composition** Ingredients Gms / Litre Casein enzymic hydrolysate 10.000 Yeast extract 5.000 Sodium chloride 10.000 Agar 15.000 Final pH ( at 25C) 7.50.2 **Formula adjusted, standardized to suit performance parameters

    Directions
    Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Dispense as desired. Mix well and and pour into sterile Petri plates.

    Principle And Interpretation


    Luria Bertani Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli . Luria Bertani Agar, Miller (2) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.

    Quality Control
    Appearance Cream to yellow homogeneous free flowing powder Gelling Firm, comparable with 1.5% Agar gel Colour and Clarity of prepared medium Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates Reaction Reaction of 4.0% w/v aqueous solution at 25C. pH : 7.50.2 Cultural Response M1151: Cultural characteristics observed after an incubation at 35-37C for 18 - 24 hours. Organism Inoculum Growth Recovery (CFU) Escherichia coli ATCC 50-100 luxuriant >=70% 23724 Escherichia coli ATCC 50-100 luxuriant >=70% 25922 Escherichia coli DH5 alpha 50-100 luxuriant >=70% MTCC 1652 Reference 1. ,,Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190. 2. Atlas R.M., 1993, Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc. Storage and Shelf Life Store below 30C and the prepared medium at 2 - 8C. Use before expiry date on the label.

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