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    Activity 12: Lipids

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    Francis Valdez
    This document describes experiments to test the properties of lipids. Lipids are composed of carbon and hydrogen and are a dense source of energy. A solubility test shows that lipids are insoluble in water but soluble in organic solvents like chloroform due to their non-polar nature. An acrolein test identifies samples containing glycerol by heating them with potassium bisulfate, which produces the pungent-smelling compound acrolein. Tests on various lipid samples like oils and lards produced positive results, confirming the presence of glycerol. In conclusion, the experiments demonstrate the solubility and identity properties of common lipid substances.

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    Activity 12: Lipids

    Uploaded by

    Francis Valdez
    49 views19 pages
    This document describes experiments to test the properties of lipids. Lipids are composed of carbon and hydrogen and are a dense source of energy. A solubility test shows that lipids are insoluble in water but soluble in organic solvents like chloroform due to their non-polar nature. An acrolein test identifies samples containing glycerol by heating them with potassium bisulfate, which produces the pungent-smelling compound acrolein. Tests on various lipid samples like oils and lards produced positive results, confirming the presence of glycerol. In conclusion, the experiments demonstrate the solubility and identity properties of common lipid substances.

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    This document describes experiments to test the properties of lipids. Lipids are composed of carbon and hydrogen and are a dense source of energy. A solubility test shows that lipids are insoluble in water but soluble in organic solvents like chloroform due to their non-polar nature. An acrolein test identifies samples containing glycerol by heating them with potassium bisulfate, which produces the pungent-smelling compound acrolein. Tests on various lipid samples like oils and lards produced positive results, confirming the presence of glycerol. In conclusion, the experiments demonstrate the solubility and identity properties of common lipid substances.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    49 views19 pages

    Activity 12: Lipids

    Uploaded by

    Francis Valdez
    This document describes experiments to test the properties of lipids. Lipids are composed of carbon and hydrogen and are a dense source of energy. A solubility test shows that lipids are insoluble in water but soluble in organic solvents like chloroform due to their non-polar nature. An acrolein test identifies samples containing glycerol by heating them with potassium bisulfate, which produces the pungent-smelling compound acrolein. Tests on various lipid samples like oils and lards produced positive results, confirming the presence of glycerol. In conclusion, the experiments demonstrate the solubility and identity properties of common lipid substances.

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    You are on page 1of 19

    ACTIVITY 12

    LIPIDS
    LIPIDS
    COMMONLY REFERRED AS ''FATS''

    - Composed of mostly carbon-hydrogen bonds (C-H) bonds.


    - Rich source energy and an efficient way for the body to store excess
    calories.
    -Also part of an integral part of the cell membranes.
    SOLUBILITY TEST
    This is based on the property of solubility of lipids in organic
    solvents and insolubility in water.

    PRINCIPLE: the oil will float on water because of lesser specific


    gravity.

    This test is used to know the solubility of lipids in some solvents,


    according to polarity feature, lipids are insoluble in polar solvents
    because lipids are non polar compounds, thus lipids are soluble in
    non
    polar solvents like chloroform, benzene and boiling alcohol.
    REAGENTS NEEDED:
    MATERIALS NEEDED:
    • Test tube holder • 10 mL Graduated
    • 10 Test Tubes Cylinder
    • 1 Test Tube Rack • Bunsen burner
    PROCEDURE:
    PROCEDURE:

    Shake the tubes and allow them to


    stand for few seconds then check if
    two phases are present.
    PROCEDURE:
    *If two phases are present,
    heat over the tube over the
    Bunsen burner flame or
    alcohol lamp for 2 mins.,
    then check again if the test
    sample is dissolved.
    PROCEDURE:
    PROCEDURE:

    *Repeat the same


    procedure using different
    solvents (Ether,
    Chloroform, water and
    ethanol). Write your
    observations
    SOLUBILITY TEST
    DCM ETHER CHLOROFORM ETHANOL WATER

    Corn Oil Soluble Soluble Soluble Soluble Insoluble


    Canola Oil Soluble Soluble Soluble Soluble Insoluble
    Pork Lard Soluble Soluble (after Soluble Soluble (after Insoluble
    heating) heating)

    Coconut Oil Soluble Soluble Soluble Soluble Insoluble


    Beef Lard Soluble (after Soluble (after Soluble Soluble (after Insoluble
    heating) heating) heating)

    Olive Oil Soluble Soluble Soluble Soluble Insoluble


    CONCLUSION
    Lipids are not dissolved in
    water (insoluble) due to their
    nature, non-polar or
    hydrophobic, but is therefore
    soluble in organic or non polar
    solvents such as choloroform,
    ethanol, DCM, and ether.
    ACROLEIN TEST
    Is used to detect glycerol or fats.
    Most lipids are found in the form of triglycerides,
    an ester formed from glycerol and fatty acids
    PRINCIPLE: When a fat is heated strongly in the
    presence of a dehydrating agent such as KSHO4
    (Potassium bisulfate), the glycerol portion of the
    molecule is dehydrated to form the unsaturated
    aldehyde, acrolein CH2= CH-CHO, which can be
    distinguished by its irritating acid smell and as
    burnt grease.
    PROCEDURE:

    Place 2 grams of KHSO4 Add 3 drops of samples in separate


    (Potassium bisulfate) test tubes
    PROCEDURE:

    Heat the test tubes for 2


    minutes and allow it to
    cool.

    *Cautiosly smell the


    characteristic odor of
    Acrolein in each tube.
    TEST SAMPLES OBSERVATION INTERPRETATION

    Corn Oil Gives pungent smell Positive

    Canola Oil Gives pungent smell Positive

    Pork Lard Gives pungent smell Positive

    Coconut Oil Gives pungent smell Positive

    Beef Lard Gives pungent smell Positive

    Olive Oil Gives pungent smell Positive


    CONCLUSION
    All of the samples
    produced pungent
    odor after heating.
    This proves that lipids
    samples contains
    glycerol.
    REFERENCES
    • http://www.magdyelnashar.com/new/images/pdf/practical.Lipids.Oil.
    Fat.Cholesterol.pdf
    • http://amrita.olabs.edu.in/?
    sub=73&brch=8&sim=210&cnt=1#:~:text=Acrolein%20test%20is
    %20used%20to,has%20a%20pungent%20irritating%20odour.
    THANKS

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