DNA Crime Scene Detective

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A CRIME HAS BEEN COMMITTED.

IT’S UP TO YOU TO TRY AND SOLVE IT.


In this activity you will take a closer look at a
crime scene, decide what evidence to collect and
how to get it back safely to the lab.

Learn more about DNA profiling and compare


different profiles to see if you can help to solve
the crime.

Good luck! © Kitchener Bain/123RF Ltd


THE CRIME SCENELook closely at this
crime scene.

What do you think has


happened here?

What could you collect


as evidence?

Make a list of samples


you would like to take
back to the lab.
LOOKING FOR DNA EVIDENCE
Think about the samples on your list. Which of these do you think contain DNA?
Write down your ideas.
YOUR NOTES HERE
HOW DO YOU ‘LEAVE DNA
BEHIND’?
We all leave DNA behind
wherever we go. DNA is a
molecule found in the
nucleus of nearly every cell
in your body. Therefore,
any sample containing cells
will contain DNA.

Think about the samples on


the previous slide. Would
you like to change any of
your answers?
SAMPLE
Forward to the ESR lab as soon as possible.
Personnel collecting DNA
Photograph the sample. samples at a crime scene need
to be very careful not to
Allow the sample to air dry before packaging into a paper bag or
collecting into a test tube if liquid.
contaminate the evidence with
their own biological material
Provide information that you think may be important, for example, an containing cells (and therefore
unusual location or any possible contaminants.
DNA).
Carefully label and seal the sample bag/container.
Think about the following
Put on protective clothing including gloves to prevent sweat, hair etc.
from contaminating the sample. steps and re-write them in
the correct order.
Carefully swab the sample.

Protect the sample from any external factors including the weather, animals
and traffic.
Polymerase chain reaction (PCR) is a method that rapidly
increases the number of copies of a target DNA sequence enabling it to be visualised. This is used for
detecting small amounts of DNA material.
The picture below explains the steps involved in the PCR process .
CREATING A DNA PROFILE

©ESR
THE SUSPECT? OR THE VICTIM?
The first step is to compare the DNA from
the blood at the scene with the two people
that live at the house. Why do you think this
is necessary?

Look at the DNA profiles.

Does the sample match either of the of


flatmates?

What does this information tell us? Write


down your ideas.
YOUR IDEAS BELOW
WHO DID IT?
After the burglary, the police interviewed neighbours and eye-witnesses who saw the
burglar run away from the house. Based on this information and some previous burglaries
in the area they have four suspects. Compare the DNA profile from the crime scene with
the suspects.

1. Is there a match?

2. What does this tell us?

3. Take notes on your ideas.


WRITE YOUR IDEAS IN THIS
SLIDE
CAN WE TRUST THE EVIDENCE?
The probability of two random individuals having the same DNA profile decreases
with each additional locus:

For example, comparing alleles at:

1 locus approximately one in 10


4 loci one in 7000
10 loci less than one in 1000 million (a US billion)
15 loci less than one in 1012 (an English billion)

ESR tests 15 loci. In the UK and Europe 10 loci are tested. In the USA 13 loci are tested.
GOING TO COURT – WHAT DO
YOU THINK?

What does a
matching DNA profile
actually prove?
What other evidence
could be used in
court in addition to a
DNA match?
ADD YOUR IDEAS BELOW

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