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    bct30306 Lect 4 Slides

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    Victor van Dam
    The document discusses biobased soft materials called oleosomes, which are lipid droplets found in plant seeds and other organisms. It describes how oleosomes can be extracted from seeds and their potential uses as delivery systems for drugs, replacing oil droplets in emulsions, or transferring their oil cores to other surfaces or organisms.

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    bct30306 Lect 4 Slides

    Uploaded by

    Victor van Dam
    15 views23 pages
    The document discusses biobased soft materials called oleosomes, which are lipid droplets found in plant seeds and other organisms. It describes how oleosomes can be extracted from seeds and their potential uses as delivery systems for drugs, replacing oil droplets in emulsions, or transferring their oil cores to other surfaces or organisms.

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    The document discusses biobased soft materials called oleosomes, which are lipid droplets found in plant seeds and other organisms. It describes how oleosomes can be extracted from seeds and their potential uses as delivery systems for drugs, replacing oil droplets in emulsions, or transferring their oil cores to other surfaces or organisms.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    15 views23 pages

    bct30306 Lect 4 Slides

    Uploaded by

    Victor van Dam
    The document discusses biobased soft materials called oleosomes, which are lipid droplets found in plant seeds and other organisms. It describes how oleosomes can be extracted from seeds and their potential uses as delivery systems for drugs, replacing oil droplets in emulsions, or transferring their oil cores to other surfaces or organisms.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    You are on page 1of 23

    Advanced Biorefinery BCT32306

    Biobased Soft Materials

    17/3/2024
    Costas Nikiforidis
    Biobased Chemistry and Technology Group
    Protein separation from a biomass
    Increase the surface
    Milling
    area
    P-9 / GR-101
    Grinding

    Seeds +
    Defatting
    Org. (for oil seeds of course)
    solvent

    Soaking
    Soften the
    (extraction seed structure P-1 / V-101
    media) Blending / Storage

    Blending Break the P-1 / DE-101


    Dead-End Filtration

    cell wall

    Sieving Solid separation

    ...Obtain proteins from the P-2 / SDR-101


    dispersion Spray Drying

    Drying
    Today’s lecture

     Biobased Soft Materials (Oleosomes)


     Scientific poster design/presentation

    3
    What are the biobased soft matter?

    • Under the umbrella of bio-inspired materials

    Design soft materials using bio-sourced molecules


    or molecular complexes

    4
    What are the biobased soft materials?

    • i.e. Cells contain many sophisticated complexes with


    different functionality
    • We are not only trying to mimic their structure and
    properties,
    but extract them and use them as such
    5
    Biobased Soft Materials
    Multidisciplinary topic:

    • Biology
    • Physics
    • Chemistry
    Oleosomes/Lipid droplets/Oil bodies
    Where can we find oleosomes?
     Present in all eukaryotic organisms
    Mammal Liver Tissue
    Mammal Neuron Tissue Insects

    Seeds Algae
    Why to focus on oleosomes?
    Proteins

    Oleosomes

    • Currently plant oils are extracted using hexane


    Oleosomes

    Oil for
    food applications

    Empty oleosomes
    after hexane extraction

    9
    Karefyllakis, et. al. Soft Matter, 2019
    Function of oleosomes
    Can oleosomes be used to replace the oil
    droplets in emulsions?

    • Should have better/different properties than current


    emulsions
    • Lower environmental impact that than oil extraction with
    hexane
    • Should not cost “too” much
    10
    Oleosome Extraction
    58 wt% yield

    80 wt% oil
    15 wt% proteins
    • pH

    Cream
    De- Soak Blend filter Liquid Centrifuge
    hull
    Subnatant

    Solids
    Pellet

    • Milling
    • Soaking time
    • Ratio solids:water
    • pH
    Extraction of oleosomes from seeds

     Optimize the extraction of oleosomes from seeds


    Extraction yield so far: up to 65 wt%
    Oleosome dispersion

    Aqueous
    Extraction

    • Maize germ
    • Sunflower seeds
    • Rapeseeds
    • Sesame seeds
    • Algae
    • Fungi
    • .....

    12
    Oleosomes in seeds

    Dry corn seed


    Jammed oleosomes with
    irregular shapes

    Spherical when dispersed


    in water
    Stable against coalescence

    13
    Nikiforidis, et. al. Food Research International, 2013
    Dense oleosomes systems
    Droplets in a
    finite space
    Φmax=0.65

    Φmax=0.96

    96 wt% oleosomes

    14
    Nikiforidis, et. al. Food Hydrocolloids, 2015
    Elasticity of oleosomes (AFM)

    Soft ball

    3 μm

    15 nm

    15
    Dynamic Molecular Simulations
    Oil Oil + PL

    No rigid PL areas

    2 ns 5 ns 7 ns 9 ns 18 ns 1000 ns
    Time
    Rearrangement of PL on surface

    16
    Ntone, et. al. Soft Matter, under review
    Loading Oleosomes for delivery systems

    Protein
    Phospholipid
    TAG matrix
    Curcumin

    +
    therapeutic

    17
    Loading of oleosomes with curcumin
    Red: Curcumin

    18

    Vardar-Kule, et. al. to be submitted


    Oleosome are metastable

     AFM
    oil

    Hydrophilic
    Mica surface
    μm

    oil
    Hydrophobic
    Silicon wafer surface

    19
    Ntone, et. al. Soft Matter, under review
    Release of oil from oleosomes on hydrophobic surface

    Silicon wafer Hydrophobic surface Hydrophobic

    20
    Ntone, et. al. Soft Matter, under review
    LDs/oleosomes transferring their core to
    bacteria

    Bosch et. al. Science, 2020

    21
    Release from oleosomes to cells

    Oleosomes

    Cells

    22
    Features of oleosomes useful for designing
    materials

     Ready to use
     Physically stable
     Carry and selective release hydrophobic molecules

    23

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