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    Micros

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    sanandresmariannekaye

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    © All Rights Reserved
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    Micros

    Uploaded by

    sanandresmariannekaye
    8 views12 pages

    Original Title

    Microscopy

    Copyright

    © © All Rights Reserved

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    Copyright:

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    Download as pptx, pdf, or txt
    8 views12 pages

    Micros

    Uploaded by

    sanandresmariannekaye

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    You are on page 1of 12

    What is a

    AMicroscope?
    microscope is an
    instrument that can be used
    to observe small objects,
    even cells. The image of
    an object is magnified
    through at least one lens in
    the microscope.
    The beginning of
    microscope
    • Zacharias Janssen is a
    Dutch reading glassmaker.
    • In 1590s, he placed two
    magnifying glasses
    together in a tube.
    • A Dutch Fabric maker in
    the 1600s
    • He made a simple
    microscope with a tiny
    glass bead for a lens
    • His microscope could
    make the image of an
    object 270 times larger
    Parts and functions of
    microscope
    STRUCTURAL COMPONENTS

    The three basic, structural components of a


    compound microscope are the head, base and
    arm.

    •Head/Body houses the optical parts in the upper part


    of the microscope
    •Base of the microscope supports the microscope and
    houses the illuminator
    •Arm connects to the base and supports the microscope
    head. It is also used to carry the microscope.
    OPTICAL COMPONENTS
    There are two optical systems in a compound
    microscope: Eyepiece Lenses and Objective Lenses:
    Eyepiece or Ocular is what you look through at the top
    of the microscope. Typically, standard eyepieces have a
    magnifying power of 10x. Optional eyepieces of varying
    powers are available, typically from 5x-30x.
    Eyepiece Tube holds the eyepieces in place above the
    objective lens. Binocular microscope heads typically
    incorporate a diopter adjustment ring that allows for the
    possible inconsistencies of our eyesight in one or both
    eyes. The monocular (single eye usage) microscope does
    not need a diopter. Binocular microscopes also swivel
    (Interpupillary Adjustment) to allow for different
    distances between the eyes of different individuals.
    Objective Lenses are the primary optical lenses on a
    microscope. They range from 4x-100x and typically,
    include, three, four or five on lens on most microscopes.
    Objectives can be forward or rear-facing.
    Nosepiece houses the objectives. The objectives are
    exposed and are mounted on a rotating turret so that
    different objectives can be conveniently selected.
    Standard objectives include 4x, 10x, 40x and 100x
    although different power objectives are available.
    Coarse and Fine Focus knobs are used to focus the
    microscope. Increasingly, they are coaxial knobs - that is
    to say they are built on the same axis with the fine focus
    knob on the outside. Coaxial focus knobs are more
    convenient since the viewer does not have to grope for a
    Stage is where the specimen to be viewed is
    placed. A mechanical stage is used when working
    at higher magnifications where delicate
    movements of the specimen slide are required.
    Stage Clips are used when there is no mechanical
    stage. The viewer is required to move the slide
    manually to view different sections of the
    specimen.
    Aperture is the hole in the stage through which the
    base (transmitted) light reaches the stage.
    Illuminator is the light source for a microscope,
    typically located in the base of the microscope.
    Most light microscopes use low voltage, halogen
    Condenser is used to collect and focus the
    light from the illuminator on to the specimen.
    It is located under the stage often in
    conjunction with an iris diaphragm.
    Iris Diaphragm controls the amount of light
    reaching the specimen. It is located above
    the condenser and below the stage. Most
    high quality microscopes include an Abbe
    condenser with an iris diaphragm. Combined,
    they control both the focus and quantity of
    light applied to the specimen.

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