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    DNA BARCODING

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    manojtbgri5793

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    DNA BARCODING

    Uploaded by

    manojtbgri5793
    9 views12 pages

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    Download as pptx, pdf, or txt
    9 views12 pages

    DNA BARCODING

    Uploaded by

    manojtbgri5793

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
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    PRESENTED BY

    VIDYA C
    VIth Sem BSc BOTANY
    BO1312
    DNA BARCODING
     It is a technique in which species
    identification is performed by using DNA
    sequences from a small fragment of the
    genome.

     DNA barcoding first came to the attention of


    the scientific community in 2003 when Paul
    Hebert’s research group at the university of
    Guelph published a paper titled ‘Biological
    identification through DNA barcodes’.
     Dr. Paul D.N. Hebert is the father of DNA
    barcoding.

     DNA barcode is the widely used computational &


    molecular based identification system.
    WHICH GENE FRAGMENTS

     Prokaryotes : rRNA genes are used for


    species identification.

     Animals,Birds& Fishes: Small regions of the


    mt COI gene.

    Plants : rbcL gene sequence & mat K gene


    sequence of chloroplast.
     Two international initiatives working towards
    the development of DNA barcoding are:

    1.CBOL
     Consortium for the Barcoding Of Life.
     Established in 2004.

    2.iBOL
     International Barcoding Of Life.
    Largest biodiversity genomes initiative.
    STEPS
    i. Collect plants, animals or products in the local
    environment or neighborhood.

    ii. Extract & purify DNA from tissue or processed


    material.

    iii. Amplify a specific region of the chloroplast ,


    mitochondrial or nuclear genome by PCR &
    analyze PCR products by gel electrophoresis.
    iv. Use the Basic Local Alignment Search Tool
    (BLAST) to identify sequences in databases.

    v. Use multiple sequence alignment to analyze


    phylogenetic relationships.

    Once the barcode sequence has been


    obtained, it is placed in the Barcode Of Life
    Data (BOLD) systems database .
    BOLD –A reference library of DNA barcodes
    that can be used to assign the identities to
    unknown specimens.
     DNA barcode sequence are very short (<1000
    bp) relative to the entire genome & they can be
    obtained reasonably quickly & cheaply.

     All the products of one type on a super market


    shelf (like a 2 l bottle Coca Cola) share exactly
    the same 11 digit barcode , which is distinct
    from all other barcodes.

     DNA barcodes vary among individuals of the


    same species , but only to a very minor degree.
     The importance of plant DNA barcode is the
    accurate species identification

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