Prevention of angiogenesis by
naked DNA IL-12 gene transfer: angioprevention by immunogene therapy.
Evaluation of the humoral immune response in BALB/c mice immunized with a
naked DNA vaccine anti- methicillin-resistant Staphylococcus aureus.
Lipid spheres can carry DNA, or
naked DNA can be injected directly into muscles.
As for safety, we are using
naked DNA delivery method instead of viral vectors to transfect genes, so that there won't be any safety concerns often caused by viral vectors in case of gene therapies.
DNA constructs encoding a chimeric T-cell receptor and a method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using
naked DNA encoding the receptor are also disclosed.
Hence when compared to direct injection of
naked DNA, gene delivery using vectors is preferred.
After an introductory article on DNA vaccines contributors offer such topics as the regulatory aspects of DNA as a pharmaceutical, plasmid manufacturing and storage, localized nucleic acid delivery, needle-less injection of
naked DNA for nonviral in vivo gene transfer, inhalation, hydrodymanic gene delivery, applications to skin diseases, electrotransfection, electrogenetransfer, and a clinical study of cancer inhibition in mice.
Methods for the transfection of gametes have employed
naked DNA, viral vectors, DNA/Liposome complexes, electroporation or high velocity microprojectiles.
He demonstrated that a different pattern of damage is observed when cells (as opposed to
naked DNA) are irradiated with UV light, and he characterized the methylation and nucleosome to the study of DNA damage at the nucleotide level.
Post: Is your trial different in the sense that you are using a different vector called
naked DNA, as opposed to the viral vector used in many other gene therapy clinical trials?
"But direct injection of
naked DNA just doesn't work," she notes.
The first method used a catheter system to inject
naked DNA for vascular endothelial growth factor directly into the myocardium of the left ventricle with a special catheter system.
Of course, the excretion of
naked DNA and DNA from dying cells can be different.
