Papers by Antonio Díaz-cruz
Transplantation Proceedings, 2017
Pesquisa Veterinaria Brasileira, Dec 1, 2017
This study was conducted to assess the changes in some indicators of oxidative status during preg... more This study was conducted to assess the changes in some indicators of oxidative status during pregnancy and lactation in sheep of different parity. Dorset x Suffolk ewes were classified by number of parity: 1, 2 and ≥4. They were sampled before pregnancy and on the first, second, third, and fourth months, then on day 143 of pregnancy, as well as on day 5 after birth and after one month of lactation. Antioxidant capacity was found to have two reductions, the first during the second month of pregnancy and the second on day 5 of lactation. Susceptibility to lipid oxidation decreased with an increased number of parturitions. A reduction in lipid oxidation was observed on day 143 of gestation relative to the other samplings during gestation and lactation. Total glutathione peroxidase activity increased when the two reductions in antioxidant capacity took place. Ascorbic acid decreased during lactation and gestation; the lowest values were recorded in the third month of gestation. It is concluded that susceptibility to lipid oxidation decreases with the number of parturitions and that in ewes, during gestation and lactation, there is a mechanism that prevents lipid oxidation involving changes in antioxidant capacity, glutathione peroxidase and ascorbic acid.
Recibido el 20 de mayo de 2004 y aceptado el 24 de noviembre de 2004. *Parte de este trabajo corr... more Recibido el 20 de mayo de 2004 y aceptado el 24 de noviembre de 2004. *Parte de este trabajo corresponde a la tesis de maestría en ciencias del primer autor. **Centro de Enseñanza, Investigación y Extensión en Producción Avícola, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Salvador Díaz Mirón s/n, 13209, México, D. F., Tel. 5845-0029. ***Departamento de Nutrición Animal y Bioquímica, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, 04510, México, D. F., Tel. 5622-5707. †Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, 04510, México, D. F. Correspondencia: Benjamín Fuente Martínez. Correo electrónico: bfuente@correo.unam.mx Salvador Díaz Mirón s/n, Col. Zapotitlán, 13209, México, D.F.,Tel. y fax: 01 (55) 5845-0029 y 5845-1530. Abstract
Biochemical and Biophysical Research Communications, 1993
Isolated rat hepatocytes, incubated in a high K+ medium which depolarizes their plasma membrane, ... more Isolated rat hepatocytes, incubated in a high K+ medium which depolarizes their plasma membrane, were used to investigate the response to adenosine. High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded that a) adenosine stimulates calcium influx in hepatocytes probably through receptor-operated Ca2+ channels which are closed by depolarization of the plasma membrane, b) the higher cytosolic calcium concentration triggers a regulatory step that fully stimulates the rate in urea synthesis.
INCI, Caracas, 2005
... ESTRÉS OXIDATIVO Y EL USO DE ANTIOXIDANTES EN ANIMALES DOMÉSTICOS. Mariana Huerta Jiménez, Ma... more ... ESTRÉS OXIDATIVO Y EL USO DE ANTIOXIDANTES EN ANIMALES DOMÉSTICOS. Mariana Huerta Jiménez, María Esther Ortega Cerrilla, Mario Cobos Peralta, José G. Herrera Haro, Antonio Díaz-Cruz y Raquel Guinzberg Perrusquía. Mariana Huerta Jiménez. ...
CiteSeerX - Document Details (Isaac Councill, Lee Giles): Inosine, an endogenous nucleoside, has ... more CiteSeerX - Document Details (Isaac Councill, Lee Giles): Inosine, an endogenous nucleoside, has recently been shown to exert potent effects on the immune, neural, and cardiovascular systems.This work addresses the modulation of intermediary metabolism by inosine through ...
Free Radical Research, 2011
The NADPH oxidase (NOX) family of enzymes oxidase catalyzes the transport of electrons from NADPH... more The NADPH oxidase (NOX) family of enzymes oxidase catalyzes the transport of electrons from NADPH to molecular oxygen and generates O(2)(•-), which is rapidly converted into H(2)O(2). We aimed to identify in hepatocytes the protein NOX complex responsible for H(2)O(2) synthesis after α(1)-adrenoceptor (α(1)-AR) stimulation, its activation mechanism, and to explore H(2)O(2) as a potential modulator of hepatic metabolic routes, gluconeogenesis, and ureagenesis, stimulated by the ARs. The dormant NOX2 complex present in hepatocyte plasma membrane (HPM) contains gp91(phox), p22(phox), p40(phox), p47(phox), p67(phox) and Rac 1 proteins. In HPM incubated with NADPH and guanosine triphosphate (GTP), α(1)-AR-mediated H(2)O(2) synthesis required all of these proteins except for p40(phox). A functional link between α(1)-AR and NOX was identified as the Gα(13) protein. Alpha(1)-AR stimulation in hepatocytes promotes Rac1-GTP generation, a necessary step for H(2)O(2) synthesis. Negative cross talk between α(1)-/β-ARs for H(2)O(2) synthesis was observed in HPM. In addition, negative cross talk of α(1)-AR via H(2)O(2) to β-AR-mediated stimulation was recorded in hepatocyte gluconeogenesis and ureagenesis, probably involving aquaporine activity. Based on previous work we suggest that H(2)O(2), generated after NOX2 activation by α(1)-AR lightening in hepatocytes, reacts with cAMP-dependent protein kinase A (PKA) subunits to form an oxidized PKA, insensitive to cAMP activation that prevented any rise in the rate of gluconeogenesis and ureagenesis.
Tropical and Subtropical Agroecosystems, 2016
The objective was to evaluate the oxidative stress (OS) and its association with performance and ... more The objective was to evaluate the oxidative stress (OS) and its association with performance and serum cortisol in dairy goats under induced (IL) and natural lactation (NL). Six goats programmed to be eliminated due to reproductive problems were IL as follows: a) days 1-7, estradiol plus progesterone; b) days 8-14, estradiol; c) days 18-20, flumetasone; d) every seven days bovine somatotropin; f) on day 21 milking began. Cortisol, antioxidant capacity and Glutathione Peroxidase activity (GHS-Px) were determined in serum. During the first two weeks in milk, NL goats had higher cortisol (P 0.05). Milk yield/day between groups (NL=2.54 + 0.2; IL= 2.12 + 0.2kg) and proportion of pregnant goats (NL=100%; IL=83.3%) were similar (P>0.05). The treatment was effective as inductor of lactation; besides most of the IL animals became pregnant despite their previous reproductive problems, prolonging their productive life with a natural lactation plus the additional benefit of at least one mor...
Ciencia E Agrotecnologia, 2018
Coffee pulp is produced in large quantities, and its disposal can pollute the environment. Howeve... more Coffee pulp is produced in large quantities, and its disposal can pollute the environment. However, the nutritional value and antioxidant content of coffee pulp make it a good option for animal feed. Therefore, the objective of this study was to determine the chemical composition, the phenolic compounds and the antioxidant capacity of coffee pulp using fresh (FCP), ensiled (ECP), and ensiled and sun-dried (EDCP) coffee pulp. The study design was completely randomized with three treatments (n=4). Dry matter (DM), crude protein (CP), ash, acid detergent fiber (ADF), neutral detergent fiber (NDF), lignin, phenolic compounds and antioxidant capacity were determined. Data were analyzed by analysis of variance, and means were compared with the Tukey test. The percentage of CP, NDF and ADF was higher in ECP and EDCP than in FCP. There were no changes in lignin content. Ensiling and sun drying did not decrease (P>0.05) caffeine or tannins. No differences were found in caffeic acid (2.031...
Tecnica Pecuaria En Mexico, 1999
Pesquisa Veterinária Brasileira
ABSTRACT: This study was conducted to assess the changes in some indicators of oxidative status d... more ABSTRACT: This study was conducted to assess the changes in some indicators of oxidative status during pregnancy and lactation in sheep of different parity. Dorset x Suffolk ewes were classified by number of parity: 1, 2 and ≥4. They were sampled before pregnancy and on the first, second, third, and fourth months, then on day 143 of pregnancy, as well as on day 5 after birth and after one month of lactation. Antioxidant capacity was found to have two reductions, the first during the second month of pregnancy and the second on day 5 of lactation. Susceptibility to lipid oxidation decreased with an increased number of parturitions. A reduction in lipid oxidation was observed on day 143 of gestation relative to the other samplings during gestation and lactation. Total glutathione peroxidase activity increased when the two reductions in antioxidant capacity took place. Ascorbic acid decreased during lactation and gestation; the lowest values were recorded in the third month of gestation...
Journal of Animal Behaviour and Biometeorology
Revista Colombiana de Ciencias Pecuarias
Oxidative status and fertility of ewes supplemented coffee pulp during estrous synchronization an... more Oxidative status and fertility of ewes supplemented coffee pulp during estrous synchronization and early pregnancy ¤ Estado oxidativo y fertilidad en ovejas suplementadas con pulpa de café durante la sincronización estral y gestación temprana
European Journal of Pharmacology, 1997
The effect of three different receptor-specific adenosine agonists on the rate of ureagenesis by ... more The effect of three different receptor-specific adenosine agonists on the rate of ureagenesis by isolated rat hepatocytes and the 2q Žw 2q x .
American Journal of Physiology-endocrinology and Metabolism, 2006
Inosine, an endogenous nucleoside, has recently been shown to exert potent effects on the immune,... more Inosine, an endogenous nucleoside, has recently been shown to exert potent effects on the immune, neural, and cardiovascular systems. This work addresses modulation of intermediary metabolism by inosine through adenosine receptors (ARs) in isolated rat hepatocytes. We conducted an in silico search in the GenBank and complete genomic sequence databases for additional adenosine/inosine receptors and for a feasible physiological role of inosine in homeostasis. Inosine stimulated glycogenolysis (approximately 40%, EC50 4.2 x 10(-9) M), gluconeogenesis (approximately 40%, EC50 7.8 x 10(-9) M), and ureagenesis (approximately 130%, EC50 7.0 x 10(-8) M) compared with basal values; these effects were blunted by the selective A3 AR antagonist 9-chloro-2-(2-furanyl)-5-[(phenylacetyl)amino][1,2,4]-triazolo[1,5-c]quinazoline (MRS 1220) but not by selective A1, A2A, and A2B AR antagonists. In addition, MRS 1220 antagonized inosine-induced transient increase (40%) in cytosolic Ca2+ and enhanced (90%) glycogen phosphorylase activity. Inosine-induced Ca2+ mobilization was desensitized by adenosine; in a reciprocal manner, inosine desensitized adenosine action. Inosine decreased the cAMP pool in hepatocytes when A1, A2A, and A2B AR were blocked by a mixture of selective antagonists. Inosine-promoted metabolic changes were unrelated to cAMP decrease but were Ca2+ dependent because they were absent in hepatocytes incubated in EGTA- or BAPTA-AM-supplemented Ca2+-free medium. After in silico analysis, no additional cognate adenosine/inosine receptors were found in human, mouse, and rat. In both perfused rat liver and isolated hepatocytes, hypoxia/reoxygenation produced an increase in inosine, adenosine, and glucose release; these actions were quantitatively greater in perfused rat liver than in isolated cells. Moreover, all of these effects were impaired by the antagonist MRS 1220. On the basis of results obtained, known higher extracellular inosine levels under ischemic conditions, and inosine's higher sensitivity for stimulating hepatic gluconeogenesis, it is suggested that, after tissular ischemia, inosine contributes to the maintenance of homeostasis by releasing glucose from the liver through stimulation of A3 ARs.
Archivos latinoamericanos de nutrición
Colostrum from Holstein cows was collected during the first three days post partum. Ground sorghu... more Colostrum from Holstein cows was collected during the first three days post partum. Ground sorghum (7.5%) was added to it. Untreated colostrum used as control, and sorghum treated colostrum samples were allowed to ferment for 0, 8 or 21 days at 18-20 degrees C in glass containers; pH, moisture, crude protein, digestible protein, ammonia, lactic acid and total energy were analyzed in untreated and treated samples. Crude protein was not significantly different (P > 0.01) in control colostrum (7.12, 5.76, 5.70%) and treated colostrum (6.66, 5.71, 5.98%) at 0, 8 and 21 days of fermentation respectively. Digestible protein was higher (P < 0.01) in the untreated (90.0, 93.0%) than in the treated colostrum (89.0, 81.0, 86.0%). Ammonia content was also higher (P < 0.01) in the control (0.25, 1.31, 1.37%) than in the treated one (0.23, 0.97, 1.20%). Lactic acid was lower (P < 0.01) in the untreated colostrum (0.82 g/100 ml) than in the treated colostrum (1.24 g/100 ml) after 21 d...
Archivos latinoamericanos de nutrición
Colostrum from Holstein cows was collected during the first three days post partum. Ground sorghu... more Colostrum from Holstein cows was collected during the first three days post partum. Ground sorghum (7.5%) was added to it. Untreated colostrum used as control, and sorghum treated colostrum samples were allowed to ferment for 0, 8 or 21 days at 18-20 degrees C in glass containers; pH, moisture, crude protein, digestible protein, ammonia, lactic acid and total energy were analyzed in untreated and treated samples. Crude protein was not significantly different (P > 0.01) in control colostrum (7.12, 5.76, 5.70%) and treated colostrum (6.66, 5.71, 5.98%) at 0, 8 and 21 days of fermentation respectively. Digestible protein was higher (P < 0.01) in the untreated (90.0, 93.0%) than in the treated colostrum (89.0, 81.0, 86.0%). Ammonia content was also higher (P < 0.01) in the control (0.25, 1.31, 1.37%) than in the treated one (0.23, 0.97, 1.20%). Lactic acid was lower (P < 0.01) in the untreated colostrum (0.82 g/100 ml) than in the treated colostrum (1.24 g/100 ml) after 21 d...
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Papers by Antonio Díaz-cruz