TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 2019
The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorou... more The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed in the serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and 18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and urea showed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in urea concentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h after exercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observed for other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, which could be used in future studies evaluating the health status of Arabian horses.
Erysipelothrix rhusiopathiae (insidiosa) is the well-known causative agent of erysipelas septicae... more Erysipelothrix rhusiopathiae (insidiosa) is the well-known causative agent of erysipelas septicaemia in swine (RADOSTITS et al., 1994). Erysipelothrix infection also occurs in sheep. The most common manifestation in sheep is polyarthritis and lameness (JUBB et d., 1985;
Milk fever and subclinical hypocalcemia are the most important macro-mineral metabolic disorders ... more Milk fever and subclinical hypocalcemia are the most important macro-mineral metabolic disorders that affect transition dairy cows. Many studies have shown that cows with subclinical hypocalce-mia are also prone to many diseases and disorders. The drain of Calcium (Ca) during early lactation represents a significant increase in Ca demand over that for late fetal growth and physiological maintenance. The requirements of the mammary gland for Ca often exceeds the ability of the cow to replenish the plasma Ca pools. Blood Ca concentrations remarkably decline in dairy cows around calving, with the lowest concentrations occurring about 12 to 24 hours after calving. To maintain Ca homeostasis after calving, at the start of lacta-tion, Ca compensating mechanisms are activated. These mechanisms involve a coordinated effort among the hormones 1,25-dihydroxyvitamin D3, parathyroid hormone (PTH), and calcitonin. Hy-pocalcemia is associated with an increased risk of several important health con...
Negative energy balance in pregnant ewes during last two month of gestation could result in fatal... more Negative energy balance in pregnant ewes during last two month of gestation could result in fatal pregnancy toxemia and affects ewe and lamb health. The understanding of variations of energy related metabolites during this critical period may cast light upon to a better management of the situation. This study describes the variations of serum energy related biochemical metabolites at a period of 45 days before to 21 days after parturition in Baloochi breed ewes. Blood from the jugular vein was collected from each sheep at days 45, 30, 20, 10, 1 prior to parturition, and days 4, 7, 13, 19 and 21 post-partum to determine total protein, albumin, urea , cholesterol, glucose, triglyceride, aspartate aminotransferase (AST), -Hydroxybutyrate (BHBA) and non-esterified fatty acid (NEFA). NEFA and BHBA concentrations reached peak levels at parturition. However, AST activity had highest levels at the first and second week after lambing. AST activity significantly correlated to energy metaboli...
The objective of this study was to determine optimal thresholds of serum concentrations of metabo... more The objective of this study was to determine optimal thresholds of serum concentrations of metabolites that were associated with an increased risk of hepatic lipidosis. Serum and liver specimens of 506 Holstein dairy cows were taken immediately after slaughter. Before slaughter, blood specimens were collected from the coccygeal vein. Serum concentrations of total bilirubin, cholesterol, triglyceride, BHBA, NEFA and activity of AST was determined and the ratio of NEFA to cholesterol was calculated. Total lipid content of liver was also measured. Metabolite concentrations in serum were considered as predictors of hepatic lipidosis in a logistic regression statistical model. The model also included BCS, parity, and cause of culling and stage of lactation as covariates. ROC analysis was used to determine the critical thresholds for predicting hepatic lipidosis. Cows with hepatic lipidosis had significantly higher concentrations of BHBA, NEFA, bilirubin, activity of AST, and NEFA to chol...
The Iranian Journal of Veterinary Science and Technology, 2014
Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by ... more Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by Theileria equi and Babesia caballi. This study was conducted to determine the seroprevalence of T. equi and B. caballi infection in Turkoman breed horses in North Khorasan Province of Iran. Blood samples were collected from 100 apparently healthy horses and examined by microscopy and indirect immunofluorescent antibody test. T. equi was microscopically detected in five blood smears. Antibodies against T. equi, B. caballi and dual infection were found in 48 (48%), 2 (2%) and 3 (3%) serum samples, respectively. No significant difference was observed between the seroprevalence of piroplasm infection with risk factors such as age, gender and activity in horses. This is the first report of detection of T. equi and B. caballi infection using IFAT in Iran. It was concluded that the seroprevalence of T. equi infection is higher than B. caballi infection in Turkoman breed horse in Iran.
The objective of the present study was to compare the efficacy of conventional and extended intra... more The objective of the present study was to compare the efficacy of conventional and extended intramammary (IMM) therapy of persistent subclinical mastitis in lactating dairy cattle using nafcillinpenicillin-dihydrostreptomycin combination (NPD). Sixty-five dairy cows with 126 infected quarters were enrolled in the study. Infected cows were allocated randomly to 1 of 3 different treatment regimens: (1) conventional group: NPD administered IMM 3 times at 24-h intervals (20 infected cows, 43 intramammary infections [IMI]), (2) extended group: NPD administered IMM 6 times at 24-h intervals (23 cows, 43 IMI), and (3) untreated control group (22 cows, 40 IMI). The overall bacteriological cure (BC) rates for subclinical IMI were 86.04%, 100%, and 20% for the conventional, extended and the control groups, respectively; indicating a higher BC rate (P 0.05). Results of this study indicate that NPD therapy was effective in eliminating subclinical IMI in lactating dairy cows, and that extended t...
The relationship between fructosamine (FRUC) and glucose (GLU) amounts in dog was previously repo... more The relationship between fructosamine (FRUC) and glucose (GLU) amounts in dog was previously reported. On the other hand, no correlations were reported in transition dairy cow and late-pregnant sheep. Similar controversy also existed concerning the relationship between FRUC, albumin (ALB), and total protein (TP) concentrations. Thirty-three healthy Holstein cows were enrolled in to the study. Blood samples were obtained by jugular venipuncture at 3 and 1 weeks before and 1 and 3 weeks after calving. The concentrations of FRUC, GLU, ALB, TP, β hydroxybutyrate (BHBA), and non-esterified fatty acids (NEFA) were measured with automated biochemical analyzer, using commercial kits. SPSS software, version 20 (SPSS Inc., Chicago, USA) was used for data analysis. The mean ± SE of fructosamine for all cows during the study was 355.6 ± 4.5 μmol/l. There was significant difference in the serum concentration of FRUC post-partum, and throughout of trial, but not at pre-partum period. The maximum concentration of FRUC was at W−1 and decreased thereafter. There were significant positive correlations between FRUC and GLU concentrations at pre-partum, post-partum, and overall time of the study. There were significant negative correlations between FRUC and NEFA concentrations at pre-partum, post-partum, and overall time of the study. There were no correlations between FRUC, TP, ALB, and BHBA amounts at pre-partum, post-partum, and overall time of the study. Linear regression analysis between each sampling amount of GLU and three consecutive FRUC concentrations did not reveal significant correlation between GLU and FRUC. According to present results, fructosamine measurement could be considered as a useful indicator of energy economy in transition period.
Seifi, H.A., Movassaghi, A.R., Mohri, M., Rad, M., Aslani, M.R., Parand, A. and Razmi, Gh. 2000. ... more Seifi, H.A., Movassaghi, A.R., Mohri, M., Rad, M., Aslani, M.R., Parand, A. and Razmi, Gh. 2000. Concurrent coccidiosis and bovine papular stomatitis infection in calves. J. Appl. Anim. Res., 18: 103–108.A group of 23 calves (2–3 months) showed diarrhoea after weaning and short distance transportation. Buccal cavity lesions of papular nature on the muzzle, gingivae, buccal papillae, inner surface of
جستجو در مقالات دانشگاهی و کتب استادان دانشگاه فردوسی مشهد. صفحه اصلی; لیست مقالات استادان; جستجو... more جستجو در مقالات دانشگاهی و کتب استادان دانشگاه فردوسی مشهد. صفحه اصلی; لیست مقالات استادان; جستجو های اخیر; بیشترین جستجو; تماس با ما. Journal of Applied Animal Research, Year (2000-9). Title : Concurrent Coccidiosis and Bovine papular.... ...
High yielding dairy cows are typically in a state of negative energy balance (NEB) postpartum, be... more High yielding dairy cows are typically in a state of negative energy balance (NEB) postpartum, because energy required for milk production and maintenance of body tissue functions exceeds energy ingested. Hence, metabolic and endocrine changes in early lactation allow enhanced mobilization of depot fat and skeletal muscle breakdown and favor partitioning of absorbed nutrients to the mammary gland to provide sufficient substrates for milk synthesis [10]. Although virtually all cows go through some degree of NEB post-calving [12], it is the degree and duration of NEB that most likely contributes to disease. Subclinical ketosis is the most common consequence of NEB in early lactation [6]. Glucocorticoids such as dexamethasone frequently are used in treatment protocols for ketosis. Glucocorticoids decrease blood ketone concentrations in cows with clinical ketosis [26, 30] and in healthy fresh cows [23]. On the other hand, it has been shown that elevated concentrations of beta hydroxybut...
The purpose of the study was to evaluate the effect of retained fetal membranes (RFM) on serum mi... more The purpose of the study was to evaluate the effect of retained fetal membranes (RFM) on serum minerals and energy-and protein-related metabolites in dairy cows at a herd with a recent history of fatty liver syndrome. Forty-seven multiparous Holstein cows were selected during transition period. Nine cows had RFM longer than 24 h after calving. Blood samples were obtained on prepartum days 21 and 7 and postpartum days 7 and 21. We used repeated measure procedure of anova to evaluate the effect of RFM on serum metabolites. Cows with RFM had significantly higher concentrations of b-hydroxybutyrate, non-esterified fatty acids and triglycerides after calving, but had lower concentrations of cholesterol during transition period. The concentrations of serum albumin and blood urea nitrogen were also significantly lower in RFMaffected cows than non-affected ones after parturition. Our results suggested that negative energy balance (NEB) postpartum was associated with RFM in dairy cattle. However, our findings did not reveal a cause and effect relationship with respect to the role of NEB as a possible risk factor for RFM.
The objective of the present study was to determine whether there was an association between the ... more The objective of the present study was to determine whether there was an association between the in vitro antimicrobial sensitivity test results of subclinical mastitis pathogens and bacteriological cure following intramammary treatment using a combination of nafcillin, penicillin, and streptomycin (NPS). Eighty-six intramammary pathogens from 43 cows were examined in this study. Most intramammary infections were due to coagulase-negative staphylococci, coliforms, and environmental streptococci. The antibiotic sensitivity to NPS was determined using the Kirby-Bauer disc diffusion method. Bacteriological cure rates for sensitive, intermediate, and resistant isolates in the standard treatment group (3 intramammary infusions of NPS once daily) were 84.4, 88.9, and 100%, respectively. These figures in the extended treatment group (6 intramammary infusions of NPS once daily) were 100% for the 3 categories. Bacteriological cure was not associated with the sensitivity test result. Based on...
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 2019
The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorou... more The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed in the serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and 18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and urea showed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in urea concentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h after exercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observed for other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, which could be used in future studies evaluating the health status of Arabian horses.
Erysipelothrix rhusiopathiae (insidiosa) is the well-known causative agent of erysipelas septicae... more Erysipelothrix rhusiopathiae (insidiosa) is the well-known causative agent of erysipelas septicaemia in swine (RADOSTITS et al., 1994). Erysipelothrix infection also occurs in sheep. The most common manifestation in sheep is polyarthritis and lameness (JUBB et d., 1985;
Milk fever and subclinical hypocalcemia are the most important macro-mineral metabolic disorders ... more Milk fever and subclinical hypocalcemia are the most important macro-mineral metabolic disorders that affect transition dairy cows. Many studies have shown that cows with subclinical hypocalce-mia are also prone to many diseases and disorders. The drain of Calcium (Ca) during early lactation represents a significant increase in Ca demand over that for late fetal growth and physiological maintenance. The requirements of the mammary gland for Ca often exceeds the ability of the cow to replenish the plasma Ca pools. Blood Ca concentrations remarkably decline in dairy cows around calving, with the lowest concentrations occurring about 12 to 24 hours after calving. To maintain Ca homeostasis after calving, at the start of lacta-tion, Ca compensating mechanisms are activated. These mechanisms involve a coordinated effort among the hormones 1,25-dihydroxyvitamin D3, parathyroid hormone (PTH), and calcitonin. Hy-pocalcemia is associated with an increased risk of several important health con...
Negative energy balance in pregnant ewes during last two month of gestation could result in fatal... more Negative energy balance in pregnant ewes during last two month of gestation could result in fatal pregnancy toxemia and affects ewe and lamb health. The understanding of variations of energy related metabolites during this critical period may cast light upon to a better management of the situation. This study describes the variations of serum energy related biochemical metabolites at a period of 45 days before to 21 days after parturition in Baloochi breed ewes. Blood from the jugular vein was collected from each sheep at days 45, 30, 20, 10, 1 prior to parturition, and days 4, 7, 13, 19 and 21 post-partum to determine total protein, albumin, urea , cholesterol, glucose, triglyceride, aspartate aminotransferase (AST), -Hydroxybutyrate (BHBA) and non-esterified fatty acid (NEFA). NEFA and BHBA concentrations reached peak levels at parturition. However, AST activity had highest levels at the first and second week after lambing. AST activity significantly correlated to energy metaboli...
The objective of this study was to determine optimal thresholds of serum concentrations of metabo... more The objective of this study was to determine optimal thresholds of serum concentrations of metabolites that were associated with an increased risk of hepatic lipidosis. Serum and liver specimens of 506 Holstein dairy cows were taken immediately after slaughter. Before slaughter, blood specimens were collected from the coccygeal vein. Serum concentrations of total bilirubin, cholesterol, triglyceride, BHBA, NEFA and activity of AST was determined and the ratio of NEFA to cholesterol was calculated. Total lipid content of liver was also measured. Metabolite concentrations in serum were considered as predictors of hepatic lipidosis in a logistic regression statistical model. The model also included BCS, parity, and cause of culling and stage of lactation as covariates. ROC analysis was used to determine the critical thresholds for predicting hepatic lipidosis. Cows with hepatic lipidosis had significantly higher concentrations of BHBA, NEFA, bilirubin, activity of AST, and NEFA to chol...
The Iranian Journal of Veterinary Science and Technology, 2014
Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by ... more Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by Theileria equi and Babesia caballi. This study was conducted to determine the seroprevalence of T. equi and B. caballi infection in Turkoman breed horses in North Khorasan Province of Iran. Blood samples were collected from 100 apparently healthy horses and examined by microscopy and indirect immunofluorescent antibody test. T. equi was microscopically detected in five blood smears. Antibodies against T. equi, B. caballi and dual infection were found in 48 (48%), 2 (2%) and 3 (3%) serum samples, respectively. No significant difference was observed between the seroprevalence of piroplasm infection with risk factors such as age, gender and activity in horses. This is the first report of detection of T. equi and B. caballi infection using IFAT in Iran. It was concluded that the seroprevalence of T. equi infection is higher than B. caballi infection in Turkoman breed horse in Iran.
The objective of the present study was to compare the efficacy of conventional and extended intra... more The objective of the present study was to compare the efficacy of conventional and extended intramammary (IMM) therapy of persistent subclinical mastitis in lactating dairy cattle using nafcillinpenicillin-dihydrostreptomycin combination (NPD). Sixty-five dairy cows with 126 infected quarters were enrolled in the study. Infected cows were allocated randomly to 1 of 3 different treatment regimens: (1) conventional group: NPD administered IMM 3 times at 24-h intervals (20 infected cows, 43 intramammary infections [IMI]), (2) extended group: NPD administered IMM 6 times at 24-h intervals (23 cows, 43 IMI), and (3) untreated control group (22 cows, 40 IMI). The overall bacteriological cure (BC) rates for subclinical IMI were 86.04%, 100%, and 20% for the conventional, extended and the control groups, respectively; indicating a higher BC rate (P 0.05). Results of this study indicate that NPD therapy was effective in eliminating subclinical IMI in lactating dairy cows, and that extended t...
The relationship between fructosamine (FRUC) and glucose (GLU) amounts in dog was previously repo... more The relationship between fructosamine (FRUC) and glucose (GLU) amounts in dog was previously reported. On the other hand, no correlations were reported in transition dairy cow and late-pregnant sheep. Similar controversy also existed concerning the relationship between FRUC, albumin (ALB), and total protein (TP) concentrations. Thirty-three healthy Holstein cows were enrolled in to the study. Blood samples were obtained by jugular venipuncture at 3 and 1 weeks before and 1 and 3 weeks after calving. The concentrations of FRUC, GLU, ALB, TP, β hydroxybutyrate (BHBA), and non-esterified fatty acids (NEFA) were measured with automated biochemical analyzer, using commercial kits. SPSS software, version 20 (SPSS Inc., Chicago, USA) was used for data analysis. The mean ± SE of fructosamine for all cows during the study was 355.6 ± 4.5 μmol/l. There was significant difference in the serum concentration of FRUC post-partum, and throughout of trial, but not at pre-partum period. The maximum concentration of FRUC was at W−1 and decreased thereafter. There were significant positive correlations between FRUC and GLU concentrations at pre-partum, post-partum, and overall time of the study. There were significant negative correlations between FRUC and NEFA concentrations at pre-partum, post-partum, and overall time of the study. There were no correlations between FRUC, TP, ALB, and BHBA amounts at pre-partum, post-partum, and overall time of the study. Linear regression analysis between each sampling amount of GLU and three consecutive FRUC concentrations did not reveal significant correlation between GLU and FRUC. According to present results, fructosamine measurement could be considered as a useful indicator of energy economy in transition period.
Seifi, H.A., Movassaghi, A.R., Mohri, M., Rad, M., Aslani, M.R., Parand, A. and Razmi, Gh. 2000. ... more Seifi, H.A., Movassaghi, A.R., Mohri, M., Rad, M., Aslani, M.R., Parand, A. and Razmi, Gh. 2000. Concurrent coccidiosis and bovine papular stomatitis infection in calves. J. Appl. Anim. Res., 18: 103–108.A group of 23 calves (2–3 months) showed diarrhoea after weaning and short distance transportation. Buccal cavity lesions of papular nature on the muzzle, gingivae, buccal papillae, inner surface of
جستجو در مقالات دانشگاهی و کتب استادان دانشگاه فردوسی مشهد. صفحه اصلی; لیست مقالات استادان; جستجو... more جستجو در مقالات دانشگاهی و کتب استادان دانشگاه فردوسی مشهد. صفحه اصلی; لیست مقالات استادان; جستجو های اخیر; بیشترین جستجو; تماس با ما. Journal of Applied Animal Research, Year (2000-9). Title : Concurrent Coccidiosis and Bovine papular.... ...
High yielding dairy cows are typically in a state of negative energy balance (NEB) postpartum, be... more High yielding dairy cows are typically in a state of negative energy balance (NEB) postpartum, because energy required for milk production and maintenance of body tissue functions exceeds energy ingested. Hence, metabolic and endocrine changes in early lactation allow enhanced mobilization of depot fat and skeletal muscle breakdown and favor partitioning of absorbed nutrients to the mammary gland to provide sufficient substrates for milk synthesis [10]. Although virtually all cows go through some degree of NEB post-calving [12], it is the degree and duration of NEB that most likely contributes to disease. Subclinical ketosis is the most common consequence of NEB in early lactation [6]. Glucocorticoids such as dexamethasone frequently are used in treatment protocols for ketosis. Glucocorticoids decrease blood ketone concentrations in cows with clinical ketosis [26, 30] and in healthy fresh cows [23]. On the other hand, it has been shown that elevated concentrations of beta hydroxybut...
The purpose of the study was to evaluate the effect of retained fetal membranes (RFM) on serum mi... more The purpose of the study was to evaluate the effect of retained fetal membranes (RFM) on serum minerals and energy-and protein-related metabolites in dairy cows at a herd with a recent history of fatty liver syndrome. Forty-seven multiparous Holstein cows were selected during transition period. Nine cows had RFM longer than 24 h after calving. Blood samples were obtained on prepartum days 21 and 7 and postpartum days 7 and 21. We used repeated measure procedure of anova to evaluate the effect of RFM on serum metabolites. Cows with RFM had significantly higher concentrations of b-hydroxybutyrate, non-esterified fatty acids and triglycerides after calving, but had lower concentrations of cholesterol during transition period. The concentrations of serum albumin and blood urea nitrogen were also significantly lower in RFMaffected cows than non-affected ones after parturition. Our results suggested that negative energy balance (NEB) postpartum was associated with RFM in dairy cattle. However, our findings did not reveal a cause and effect relationship with respect to the role of NEB as a possible risk factor for RFM.
The objective of the present study was to determine whether there was an association between the ... more The objective of the present study was to determine whether there was an association between the in vitro antimicrobial sensitivity test results of subclinical mastitis pathogens and bacteriological cure following intramammary treatment using a combination of nafcillin, penicillin, and streptomycin (NPS). Eighty-six intramammary pathogens from 43 cows were examined in this study. Most intramammary infections were due to coagulase-negative staphylococci, coliforms, and environmental streptococci. The antibiotic sensitivity to NPS was determined using the Kirby-Bauer disc diffusion method. Bacteriological cure rates for sensitive, intermediate, and resistant isolates in the standard treatment group (3 intramammary infusions of NPS once daily) were 84.4, 88.9, and 100%, respectively. These figures in the extended treatment group (6 intramammary infusions of NPS once daily) were 100% for the 3 categories. Bacteriological cure was not associated with the sensitivity test result. Based on...
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