Objective In this study, we aimed to investigate serum chitotriosidase (ChT) activity and irisin ... more Objective In this study, we aimed to investigate serum chitotriosidase (ChT) activity and irisin levels in children with obesity and compare them to those of healthy counterparts. A total of 91 obese and 83 normal-weight children were included in the study. Serum ChT activity and irisin levels of children with obesity were compared to those of normal-weight children. The mean ChT value in the obese group was 1825.332 ± 4804.147 nmol/L/h and was significantly higher than that in the control group. In contrast, the mean irisin level, 2679.663 ± 5473.58 pg/ml, was lower than that in the control group. The cutoff point of the continuous variable selected in the model for ChT was 601, with 31.9% sensitivity, 90.6% specificity, and an area under the curve of 0.596. The cutoff point for irisin was 901.4, with 83.5% sensitivity, 42.4% specificity, and an area under the curve of 0.617. This study is the first to show decreased serum both serum ChT activity and irisin level and ChT's association with irisin levels in children with obesity . We argue that ChT and irisin should be considered potential biomarkers of metabolic syndrome in children with obesity.
Objective: The aim of the present study was to evaluate the relationship between adipokines and p... more Objective: The aim of the present study was to evaluate the relationship between adipokines and perilipin (PLIN) polymorphisms with non-alcoholic fatty liver disease (NAFLD). Methods: Obese Turkish adolescents were assessed in the study. The patients were divided into two groups: obese (NAFLD and non-NAFLD) and non-obese. Serum leptin, adiponectin, resistin, and ghrelin levels and PLIN gene analysis (PLIN 1, 4, and 6) were studied in all patients and healthy control group. Data obtained were compared with those of healthy control group. Results: Overall, 83 obese adolescents with NAFLD, 123 obese adolescents with normal liver, and 102 healthy non-obese adolescents as the control group were evaluated. No significant difference was observed in terms of serum adipokine (leptin, adiponectin, resistin, and ghrelin) levels in patients with NAFLD and non-NAFLD obese adolescents. The incidence of major alleles of PLIN 6 genotype in obese adolescents without NAFLD was slightly higher than that in the control group (p=0.06). PLIN 6 minor allele incidence was significantly lower (p=0.01), and PLIN 4 major allele frequency in patients with NAFLD was slightly lower than those in the control group (p=0.05). Conclusion: These findings suggested that no adipokine role was determined in the development of fatty liver in obese adolescents. The low rate in PLIN 6 minor allele can be a risk factor for NAFLD in adolescents.
Bu çalışmada, gebelikleri süresince yeşil çay ekstraktıyla beslenen sıçanların ve yavrularının ka... more Bu çalışmada, gebelikleri süresince yeşil çay ekstraktıyla beslenen sıçanların ve yavrularının karaciğer dokularında sSitokeratin-18 (SK-18) düzey ve ekspresyonunu değerlendirmek amaçlanmıştır. Yöntem: On sekiz adet Wistar albino gebe sıçan, iki gruba ayrıldı: kontrol grubu ve (oral gavaj ile 50 mg/kg yeşil çay ekstraktı verilenuygulanan) yeşil çay grubu. Yirmi bir günlük gebelikten sonra, her iki gruptaki anne sıçanların ve doğdukları ilk gün yavruların karaciğer dokuları çıkarıldı. Bu doku örneklerinde SK-18 ekspresyonu ve düzeyi immünohistokimyasal olarak ve enzime bağlı immünosorbent analiz (ELISA) ile değerlendirildi. Bulgular: İki grupta da maternal dokularda santral venlerin çevresindeki hepatositlerin hücre zarları yakınında kuvvetli SK-18 immünoreaksiyonu gözlendi. Kontrol grubu yenidoğan dokularında santral ven çevresindeki hepatositlerde zayıf SK-18 immünoreaksiyonu gözlenirken, yeşil çay grubunda hepatositlerin hücre zarı yakınında oldukça kuvvetli SK-18 immünoreaksiyonu gözlendi. Biyokimyasal incelemede de, maternal SK-18 düzeyleri her iki grupta da yüksek olup birbirinden istatistiksel olarak anlamlı farklılık göstermezken, neonatal SK-18 düzeyleri kontrol grubuna kıyasla yeşil çay grubunda anlamlı biçimde daha yüksekti. Sonuç: İmmünohistokimya ve ELISA sonuçlarımız gebelik süresince maternal yeşil çay tüketiminin yenidoğan karaciğerinde hücre hasarına neden olabileceğini düşündürmektedir.
The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the ... more The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T] were studied by Real Time-PCR. Serum leptin, adiponectin, resistin and ghrelin levels were studied by ELISA method in both groups and their link with perilipin polymorphisms were analyzed. Serum leptin level was found significantly high in obese adolescents. Other adipokine levels were similar in both groups. The incidence of PLIN 1, PLIN 4, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T minor and major alleles was similar in both groups. PLIN 6 T/T allele was determined significantly high in obese adolescents compared to that of control group. ...
Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile kar... more Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile karakterize olan oksidatif stres, endotel tabakasının işlev bozukluğunun temel nedenidir. Oksidatif stres kardiyovasküler hastalıklar, kanser, nörolojik hastalıklar, diyabet, yaşlanma ve diğer pek çok hastalığın patogenezinde önemli bir rol oynamaktadır. Oksidatif stres ile ilgili savunma mekanizmaları; bu mekanizmaların engellenmesi, onarımı, fiziksel savunma ve antioksidan savunmayı kapsamaktadır. Hipoksi-reoksijenasyon (ya da iskemi-reperfüzyon) hasarı kalpte hem koroner damarları (damar düz kası ve endotel), hem de kalp kası hücrelerini (kardiyak miyositleri) etkilemektedir. Koroner damarlardaki hasar, damar tonusunu değiştirebilir ve reperfüzyon sırasında koroner kan akışını etkileyebilir. Kas hücrelerindeki iskemi-reperfüzyon hasarına ek olarak, koroner akışın azalması miyokardiyal disfonksiyonu arttırmakta, bu da ölüm ve hastalıkla sonuçlanmaktadır. Enzimatik antioksidan savunma süperoksit dismutaz, glutatyon peroksidaz ve katalazı kapsamaktadır. Enzimatik olmayan antioksidanlar askorbik asit, α-tokoferol, glutatyon, karotenoidler, flavonoidler, stilbenler ve diğerleridir. Normal koşullar altında bu antioksidanların hücre içi düzeyi ve etkinlikleri arasında denge bulunmaktadır. Bu denge, organizmanın canlılığı ve sağlığı açısından gereklidir. Resveratrol, stilbenlerin alt grubu olup, üzüm ve kırmızı şarapta bulunan polifenolik bir bileşiktir. Anti-inflamatuvar, antioksidan, anti-apoptotik, sitoprotektif, anti-kanser ve kardioprotektif etkileri bulunmaktadır. Fransa'da kırmızı şarap tüketimi ile kardiovasküler hastalık sıklığı arasında ters orantı saptanmıştır (Fransız paradoksu). Kardiyak hasarın akut ve kronik modellerinde, resveratrol miyokardiyal iskemireperfüzyon hasarının şiddetini azaltmaktadır. Kardiyovasküler hastalıkların patofizyolojisinde rol oynayan oksidatif hasara karşı koruyucu olduğu, yapılan çok sayıda çalışma ile gösterilmiştir.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p<0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p<0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary artery endothelial cell damage. It is thought that these results could pave the way to the new therapeutic approaches to protect against oxidative stress that develops in cardiovascular diseases.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p < 0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p < 0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary artery endothelial cell damage. It is thought that these results could pave the way to the new therapeutic approaches to protect against oxidative stress that develops in cardiovascular diseases.
Journal of Pediatric Endocrinology and Metabolism, 2015
The prevalence of obesity and related cardiovascular comorbodities is increasing rapidly. Adipoki... more The prevalence of obesity and related cardiovascular comorbodities is increasing rapidly. Adipokines play a major role in the pathogenesis of obesity-related inflammation and hypertension. The aim of this study was to evaluate the serum adropin levels in obese children and to determine the relationship between adropin levels and blood pressure (BP) in the pediatric age group. Forty obese children (mean age: 12.5±2.5 years; male/female ratio: 18/22) and 15 healthy controls (mean age: 15±3.14 years; male/female ratio: 5/15) were included in the study. Serum adropin levels, and a number of laboratory and clinical variables were compared. Ambulatory blood pressure monitoring was performed on obese subjects. Relationship between adropin levels and BP variables was examined. Serum adropin levels were significantly lower in obese subjects than in healthy controls (193.56±94 vs. 289±187 pg/mL, p=0.03). Adropin levels were correlated negatively with body mass index z-score (r=-0.56; p=0.034). There was no correlation between serum adropin levels and laboratory variables in obese subjects. Five of the patients (12.5%) were nondipper, and nine of the patients (22.5%) had hypertension. There was no significant correlation between serum adropin levels and BP variables. Serum adropin levels were significantly lower in obese children; however, there was no correlation between serum adropin levels and BP variables. Further studies are needed to determine the role of adipokines on BP.
Traumatic brain injury (TBI) is a major health problem in pediatric ages and also has major socia... more Traumatic brain injury (TBI) is a major health problem in pediatric ages and also has major social, economic, and emotional outcomes, with diverse sequelae in many spheres of everyday life. We aimed to investigate the effect of MK-801, a competitive NMDA receptor antagonist, on hippocampal damage and behavioral deficits on 10-day-old rat pups subjected to contusion injury. The aims of the present study were to determine: (i) the short term effects of MK-801 on hippocampal BDNF, NGF and NMDA receptor immunoreactivity and neuron density in hippocampus (ii) long term effects of MK-801 on cognitive dysfunction following TBI in the immature rats. MK-801, was injected intraperitoneally at the doses of 1mg/kg of body weight immediately after induction of traumatic injury. Hippocampal damage was examined by cresyl violet staining, BDNF, NGF and NMDAR receptor immunohistochemistry on P10 day and behavioral alterations were evaluated using elevated plus maze and novel object recognition tests two months after the trauma. Histopathological and immunohistochemical evaluations showed that treatment with a single dose of 1mg/kg MK-801 (i.p.) significantly ameliorated the trauma induced hippocampal neuron loss and decreased BDNF, NGF and NMDAR expressions in CA1, CA3 and DG hippocampal brain regions. Additionally, treatment with MK-801 ameliorated anxiety and hippocampus dependent memory of animals subjected to trauma. These results show that acute treatment of MK-801 has a neuroprotective role against trauma induced hippocampal neuron loss and associated cognitive impairment in immature rats.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p < 0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p < 0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary ar...
Journal of the Turkish German Gynecological Association, 2013
To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by ... more To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemistry in a rat uterine horn model with full thickness injury. Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically. Adhesion scores were 2.00±0.81, 2.14±0.69 0.71±0.75, and 0.85±0.69 for extent and 2.28±0.48, 2.14±0.69, 0.85±0.69, and 1.14±0.69 for severity in Groups 1, 2, 3 and 4, respectively. Adhesion extent and severity were significantly less for groups treated by lipoic acid but no difference was observed between long and short administration. Both Vitronectin and u-PAR staining were significantly increased in treatment groups when compared to the control group. Lipoic acid was found to be effective in reducing postoperative adhesion formation in a rat model.
Journal of Pediatric Endocrinology and Metabolism, 2014
Nonalcoholic fatty liver disease (NAFLD) is the accumulation of excess fat in the liver in the ab... more Nonalcoholic fatty liver disease (NAFLD) is the accumulation of excess fat in the liver in the absence of alcohol consumption, which is commonly associated with obesity and increased risk of atherosclerosis as well as insulin resistance. Adropin is a recently identified protein encoded by the gene related with energy homeostasis, which is expressed in the liver and the brain and has a role in preventing insulin resistance and obesity. The aim of this study was to investigate the serum adropin and leptin levels in obese adolescents and compare the patients with, and without, NAFLD and with healthy controls. Sixty-four obese adolescents (30 with NAFLD, 34 without NAFLD) and 36 healthy controls were enrolled in the study. Serum adropin and leptin levels were evaluated by sandwich enzyme-linked immunosorbent assay. Serum adropin levels were significantly lower in obese children than healthy controls (3.2±1.0 and 9.2±1.2 ng/mL, respectively, p=0.001). Serum leptin levels were significantly higher in patients than in controls (12.4±1.1 and 4.1±3.1 pg/mL, respectively; p=0.000). Serum adropin levels of patients with NAFLD were significantly lower than in patients without NAFLD (2.9±0.5 and 3.5±1.2 ng/mL, respectively; p=0.023) and healthy controls (p=0.000). Logistic regression analysis showed that a decrease in adropin levels was the only independent factor for fatty liver disease in obese adolescents (odds ratio: 3.07, 95% confidence interval 1.14-8.2, p=0.026). Leptin, relative weight and HOMA-IR of the patients were not independent risk factors for NAFLD. In this study, serum adropin levels were significantly lower in obese adolescents with fatty liver disease compared to patients without fatty liver disease and healthy controls. Lower adropin level was an independent risk factor for NAFLD in obese adolescents in logistic regression analysis. Assessment of serum adropin concentrations may provide a reliable indicator of fatty liver disease in obese adolescents.
The objective of this study was to investigate the effect of resveratrol on the healing process a... more The objective of this study was to investigate the effect of resveratrol on the healing process after midline laparotomy in rats. The study was performed on adult female Wistar-Albino rats. The study group was orally administered 0.5 mg/kg resveratrol once a day for 7 days before the operation until 12 h before surgery and then the treatment was maintained throughout the study. Each rat was anesthetized, and a 4-cm midline laparotomy was performed. Ten animals in each group were sacrificed on postoperative days 7, and 14. A tensile strength analysis was performed, hydroxyproline levels were measured, and the abdominal incision wounds were examined histologically. Resveratrol administration significantly increased the tensile strength of the abdominal fascia, and increased the hydroxyproline levels on postoperative day 14. The acute inflammation scores, collagen deposition scores and the neovascularization scores on postoperative days 7 and 14 were found to be significantly higher in the resveratrol treatment group compared to the control group. The amount of granulation tissue and the fibroblast maturation scores were found to be significantly higher only on postoperative day 14 in the treatment group compared to the control group. Our findings show that resveratrol may have a beneficial effect on incisional wound healing.
Journal of Pediatric Endocrinology and Metabolism, 2000
Nonalcoholic fatty liver disease (NAFLD) is associated with inflammation and increased risk of at... more Nonalcoholic fatty liver disease (NAFLD) is associated with inflammation and increased risk of atherosclerosis. Neopterin is regarded as a biochemical marker of cell-mediated immunity, which is secreted by monocytes and macrophages, mainly in response to interferon-gamma. The aim of the present study was to investigate the serum neopterin levels in obese adolescents and compare the neopterin levels in patients with and without NAFLD and also with healthy controls. The second aim of the study was to research the possible relationship between neopterin levels and adipokines (leptin, adiponectin, resistin, and ghrelin). Ninety-three obese adolescents (39 with NAFLD, 54 without NAFLD) and 55 healthy controls were enrolled in the study. Serum levels of neopterin and adipokines were measured with high-performance liquid chromatography and sandwich enzyme-linked immunosorbent assay, respectively. Serum neopterin levels were significantly higher in patients with NAFLD (3.20 ± 0.09 nmol/L) than in their healthy peers (2.91 ± 0.08 nmol/L) (p=0.020). Neopterin levels were positively correlated with leptin levels in obese patients (r=0.380, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) and in the group comprising all individuals (r=0.206, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). There was no correlation between neopterin concentrations and relative weight, alanin aminotransferase, adiponectin, resistin, and ghrelin levels. The serum neopterin levels were significantly higher in obese adolescents with fatty liver disease compared to controls, and this may be related to increased cell-mediated immunity in fatty liver disease.
Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immun... more Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.
Evidence-Based Complementary and Alternative Medicine, 2013
This study compared the effects of transcutaneous electrical nerve stimulation (TENS), saline sol... more This study compared the effects of transcutaneous electrical nerve stimulation (TENS), saline solution (SS), povidone-iodine (PI), and lavender oil (Lavandula angustifolia) through expression of growth factors in a rat model of wound healing. Six experimental groups were established, each containing 8 rats: a healthy group with no incision wounds, an incision-control group, an incision and TENS group, an incision and SS group, an incision and PI group, and an incision and lavender oil group. Experiments continued for 5 days, after which the skin in the excision area was removed. Tissue concentrations of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-A were measured using enzyme-linked immunosorbent assay (ELISA). Tissue expressions of EGF, PDGF-A, and fibroblast growth factor (FGF)-2 were determined using immunohistochemistry. Wound closure progressed more rapidly in the TENS and lavender oil groups than in the control and other study groups. In particular, PDGF-A expressions in the dermis and EGF expression in the epidermis were significantly intense in the TENS group ( < 0.05). In addition, ELISA levels of growth factors such as PDGF-A and EGF were significantly higher in TENS group compared to the control group ( < 0.05). These immunohistochemical and ELISA results suggest that TENS may improve wound healing through increasing growth factors in the dermis and epidermis more than other topical applications.
Türk Biyokimya Dergisi [Turkish Journal of …, 2008
Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile kar... more Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile karakterize olan oksidatif stres, endotel tabakasının işlev bozukluğunun temel nedenidir. Oksidatif stres kardiyovasküler hastalıklar, kanser, nörolojik hastalıklar, diyabet, yaşlanma ve diğer pek çok hastalığın patogenezinde önemli bir rol oynamaktadır. Oksidatif stres ile ilgili savunma mekanizmaları; bu mekanizmaların engellenmesi, onarımı, fiziksel savunma ve antioksidan savunmayı kapsamaktadır. Hipoksi-reoksijenasyon (ya da iskemi-reperfüzyon) hasarı kalpte hem koroner damarları (damar düz kası ve endotel), hem de kalp kası hücrelerini (kardiyak miyositleri) etkilemektedir. Koroner damarlardaki hasar, damar tonusunu değiştirebilir ve reperfüzyon sırasında koroner kan akışını etkileyebilir. Kas hücrelerindeki iskemi-reperfüzyon hasarına ek olarak, koroner akışın azalması miyokardiyal disfonksiyonu arttırmakta, bu da ölüm ve hastalıkla sonuçlanmaktadır. Enzimatik antioksidan savunma süperoksit dismutaz, glutatyon peroksidaz ve katalazı kapsamaktadır. Enzimatik olmayan antioksidanlar askorbik asit, α-tokoferol, glutatyon, karotenoidler, flavonoidler, stilbenler ve diğerleridir. Normal koşullar altında bu antioksidanların hücre içi düzeyi ve etkinlikleri arasında denge bulunmaktadır. Bu denge, organizmanın canlılığı ve sağlığı açısından gereklidir. Resveratrol, stilbenlerin alt grubu olup, üzüm ve kırmızı şarapta bulunan polifenolik bir bileşiktir. Anti-inflamatuvar, antioksidan, anti-apoptotik, sitoprotektif, anti-kanser ve kardioprotektif etkileri bulunmaktadır. Fransa'da kırmızı şarap tüketimi ile kardiovasküler hastalık sıklığı arasında ters orantı saptanmıştır (Fransız paradoksu). Kardiyak hasarın akut ve kronik modellerinde, resveratrol miyokardiyal iskemireperfüzyon hasarının şiddetini azaltmaktadır. Kardiyovasküler hastalıkların patofizyolojisinde rol oynayan oksidatif hasara karşı koruyucu olduğu, yapılan çok sayıda çalışma ile gösterilmiştir.
International Journal for Vitamin and Nutrition Research, 2012
The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular u... more The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Among the human matrix metalloproteinases (MMPs), MMP-2 and -9, known as gelatinases, are the key enzymes for degrading type IV collagen, which is the major component of the basal membrane that surrounds the cerebral blood vessel. In the present study, we investigated the effects of resveratrol on cytotoxicity, reactive oxygen species (ROS), and gelatinases (MMP-2 and -9) in human cerebral microvascular endothelial cells exposed to 6 hours of oxygen-glucose deprivation and a subsequent 24 hours of reoxygenation with glucose (OGD/R), to mimic ischemia/reperfusion in vivo. Lactate dehydrogenase increased significantly, in comparison to that in the normoxia group. ROS was markedly increased in the OGD/R group, compared to normoxia. Correspondingly, ROS was significantly reduced with 50 μM of resveratrol. The proMMP-2 activity in the OGD/R group showed a statistically significant increase from the control cells. Resveratrol preconditioning decreased significantly the proMMP-2 in the cells exposed to OGD/R in comparison to that in the OGD/R group. Our results indicate that resveratrol regulates MMP-2 activity induced by OGD/R via its antioxidant effect, implying a possible mechanism related to the neuroprotective effect of resveratrol.
Objective In this study, we aimed to investigate serum chitotriosidase (ChT) activity and irisin ... more Objective In this study, we aimed to investigate serum chitotriosidase (ChT) activity and irisin levels in children with obesity and compare them to those of healthy counterparts. A total of 91 obese and 83 normal-weight children were included in the study. Serum ChT activity and irisin levels of children with obesity were compared to those of normal-weight children. The mean ChT value in the obese group was 1825.332 ± 4804.147 nmol/L/h and was significantly higher than that in the control group. In contrast, the mean irisin level, 2679.663 ± 5473.58 pg/ml, was lower than that in the control group. The cutoff point of the continuous variable selected in the model for ChT was 601, with 31.9% sensitivity, 90.6% specificity, and an area under the curve of 0.596. The cutoff point for irisin was 901.4, with 83.5% sensitivity, 42.4% specificity, and an area under the curve of 0.617. This study is the first to show decreased serum both serum ChT activity and irisin level and ChT's association with irisin levels in children with obesity . We argue that ChT and irisin should be considered potential biomarkers of metabolic syndrome in children with obesity.
Objective: The aim of the present study was to evaluate the relationship between adipokines and p... more Objective: The aim of the present study was to evaluate the relationship between adipokines and perilipin (PLIN) polymorphisms with non-alcoholic fatty liver disease (NAFLD). Methods: Obese Turkish adolescents were assessed in the study. The patients were divided into two groups: obese (NAFLD and non-NAFLD) and non-obese. Serum leptin, adiponectin, resistin, and ghrelin levels and PLIN gene analysis (PLIN 1, 4, and 6) were studied in all patients and healthy control group. Data obtained were compared with those of healthy control group. Results: Overall, 83 obese adolescents with NAFLD, 123 obese adolescents with normal liver, and 102 healthy non-obese adolescents as the control group were evaluated. No significant difference was observed in terms of serum adipokine (leptin, adiponectin, resistin, and ghrelin) levels in patients with NAFLD and non-NAFLD obese adolescents. The incidence of major alleles of PLIN 6 genotype in obese adolescents without NAFLD was slightly higher than that in the control group (p=0.06). PLIN 6 minor allele incidence was significantly lower (p=0.01), and PLIN 4 major allele frequency in patients with NAFLD was slightly lower than those in the control group (p=0.05). Conclusion: These findings suggested that no adipokine role was determined in the development of fatty liver in obese adolescents. The low rate in PLIN 6 minor allele can be a risk factor for NAFLD in adolescents.
Bu çalışmada, gebelikleri süresince yeşil çay ekstraktıyla beslenen sıçanların ve yavrularının ka... more Bu çalışmada, gebelikleri süresince yeşil çay ekstraktıyla beslenen sıçanların ve yavrularının karaciğer dokularında sSitokeratin-18 (SK-18) düzey ve ekspresyonunu değerlendirmek amaçlanmıştır. Yöntem: On sekiz adet Wistar albino gebe sıçan, iki gruba ayrıldı: kontrol grubu ve (oral gavaj ile 50 mg/kg yeşil çay ekstraktı verilenuygulanan) yeşil çay grubu. Yirmi bir günlük gebelikten sonra, her iki gruptaki anne sıçanların ve doğdukları ilk gün yavruların karaciğer dokuları çıkarıldı. Bu doku örneklerinde SK-18 ekspresyonu ve düzeyi immünohistokimyasal olarak ve enzime bağlı immünosorbent analiz (ELISA) ile değerlendirildi. Bulgular: İki grupta da maternal dokularda santral venlerin çevresindeki hepatositlerin hücre zarları yakınında kuvvetli SK-18 immünoreaksiyonu gözlendi. Kontrol grubu yenidoğan dokularında santral ven çevresindeki hepatositlerde zayıf SK-18 immünoreaksiyonu gözlenirken, yeşil çay grubunda hepatositlerin hücre zarı yakınında oldukça kuvvetli SK-18 immünoreaksiyonu gözlendi. Biyokimyasal incelemede de, maternal SK-18 düzeyleri her iki grupta da yüksek olup birbirinden istatistiksel olarak anlamlı farklılık göstermezken, neonatal SK-18 düzeyleri kontrol grubuna kıyasla yeşil çay grubunda anlamlı biçimde daha yüksekti. Sonuç: İmmünohistokimya ve ELISA sonuçlarımız gebelik süresince maternal yeşil çay tüketiminin yenidoğan karaciğerinde hücre hasarına neden olabileceğini düşündürmektedir.
The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the ... more The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T] were studied by Real Time-PCR. Serum leptin, adiponectin, resistin and ghrelin levels were studied by ELISA method in both groups and their link with perilipin polymorphisms were analyzed. Serum leptin level was found significantly high in obese adolescents. Other adipokine levels were similar in both groups. The incidence of PLIN 1, PLIN 4, PLIN 5'UTR-1234 C > G and PLIN 10171 A/T minor and major alleles was similar in both groups. PLIN 6 T/T allele was determined significantly high in obese adolescents compared to that of control group. ...
Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile kar... more Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile karakterize olan oksidatif stres, endotel tabakasının işlev bozukluğunun temel nedenidir. Oksidatif stres kardiyovasküler hastalıklar, kanser, nörolojik hastalıklar, diyabet, yaşlanma ve diğer pek çok hastalığın patogenezinde önemli bir rol oynamaktadır. Oksidatif stres ile ilgili savunma mekanizmaları; bu mekanizmaların engellenmesi, onarımı, fiziksel savunma ve antioksidan savunmayı kapsamaktadır. Hipoksi-reoksijenasyon (ya da iskemi-reperfüzyon) hasarı kalpte hem koroner damarları (damar düz kası ve endotel), hem de kalp kası hücrelerini (kardiyak miyositleri) etkilemektedir. Koroner damarlardaki hasar, damar tonusunu değiştirebilir ve reperfüzyon sırasında koroner kan akışını etkileyebilir. Kas hücrelerindeki iskemi-reperfüzyon hasarına ek olarak, koroner akışın azalması miyokardiyal disfonksiyonu arttırmakta, bu da ölüm ve hastalıkla sonuçlanmaktadır. Enzimatik antioksidan savunma süperoksit dismutaz, glutatyon peroksidaz ve katalazı kapsamaktadır. Enzimatik olmayan antioksidanlar askorbik asit, α-tokoferol, glutatyon, karotenoidler, flavonoidler, stilbenler ve diğerleridir. Normal koşullar altında bu antioksidanların hücre içi düzeyi ve etkinlikleri arasında denge bulunmaktadır. Bu denge, organizmanın canlılığı ve sağlığı açısından gereklidir. Resveratrol, stilbenlerin alt grubu olup, üzüm ve kırmızı şarapta bulunan polifenolik bir bileşiktir. Anti-inflamatuvar, antioksidan, anti-apoptotik, sitoprotektif, anti-kanser ve kardioprotektif etkileri bulunmaktadır. Fransa'da kırmızı şarap tüketimi ile kardiovasküler hastalık sıklığı arasında ters orantı saptanmıştır (Fransız paradoksu). Kardiyak hasarın akut ve kronik modellerinde, resveratrol miyokardiyal iskemireperfüzyon hasarının şiddetini azaltmaktadır. Kardiyovasküler hastalıkların patofizyolojisinde rol oynayan oksidatif hasara karşı koruyucu olduğu, yapılan çok sayıda çalışma ile gösterilmiştir.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p<0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p<0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary artery endothelial cell damage. It is thought that these results could pave the way to the new therapeutic approaches to protect against oxidative stress that develops in cardiovascular diseases.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p < 0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p < 0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary artery endothelial cell damage. It is thought that these results could pave the way to the new therapeutic approaches to protect against oxidative stress that develops in cardiovascular diseases.
Journal of Pediatric Endocrinology and Metabolism, 2015
The prevalence of obesity and related cardiovascular comorbodities is increasing rapidly. Adipoki... more The prevalence of obesity and related cardiovascular comorbodities is increasing rapidly. Adipokines play a major role in the pathogenesis of obesity-related inflammation and hypertension. The aim of this study was to evaluate the serum adropin levels in obese children and to determine the relationship between adropin levels and blood pressure (BP) in the pediatric age group. Forty obese children (mean age: 12.5±2.5 years; male/female ratio: 18/22) and 15 healthy controls (mean age: 15±3.14 years; male/female ratio: 5/15) were included in the study. Serum adropin levels, and a number of laboratory and clinical variables were compared. Ambulatory blood pressure monitoring was performed on obese subjects. Relationship between adropin levels and BP variables was examined. Serum adropin levels were significantly lower in obese subjects than in healthy controls (193.56±94 vs. 289±187 pg/mL, p=0.03). Adropin levels were correlated negatively with body mass index z-score (r=-0.56; p=0.034). There was no correlation between serum adropin levels and laboratory variables in obese subjects. Five of the patients (12.5%) were nondipper, and nine of the patients (22.5%) had hypertension. There was no significant correlation between serum adropin levels and BP variables. Serum adropin levels were significantly lower in obese children; however, there was no correlation between serum adropin levels and BP variables. Further studies are needed to determine the role of adipokines on BP.
Traumatic brain injury (TBI) is a major health problem in pediatric ages and also has major socia... more Traumatic brain injury (TBI) is a major health problem in pediatric ages and also has major social, economic, and emotional outcomes, with diverse sequelae in many spheres of everyday life. We aimed to investigate the effect of MK-801, a competitive NMDA receptor antagonist, on hippocampal damage and behavioral deficits on 10-day-old rat pups subjected to contusion injury. The aims of the present study were to determine: (i) the short term effects of MK-801 on hippocampal BDNF, NGF and NMDA receptor immunoreactivity and neuron density in hippocampus (ii) long term effects of MK-801 on cognitive dysfunction following TBI in the immature rats. MK-801, was injected intraperitoneally at the doses of 1mg/kg of body weight immediately after induction of traumatic injury. Hippocampal damage was examined by cresyl violet staining, BDNF, NGF and NMDAR receptor immunohistochemistry on P10 day and behavioral alterations were evaluated using elevated plus maze and novel object recognition tests two months after the trauma. Histopathological and immunohistochemical evaluations showed that treatment with a single dose of 1mg/kg MK-801 (i.p.) significantly ameliorated the trauma induced hippocampal neuron loss and decreased BDNF, NGF and NMDAR expressions in CA1, CA3 and DG hippocampal brain regions. Additionally, treatment with MK-801 ameliorated anxiety and hippocampus dependent memory of animals subjected to trauma. These results show that acute treatment of MK-801 has a neuroprotective role against trauma induced hippocampal neuron loss and associated cognitive impairment in immature rats.
Oxidative stress is defined as imbalance between the production and destruction of reactive oxyge... more Oxidative stress is defined as imbalance between the production and destruction of reactive oxygen species. The aim of this study was to investigate whether resveratrol could protect human endothelial cells against hydrogen peroxide damage in vitro. In this in vitro study on human coronary endothelial cells, the effects of resveratrol on the glutathione content in human coronary endothelial cells in vitro were evaluated with high performance liquid chromatography. The effects of resveratrol on protein expression of the glutamate cysteine ligase, glutathione peroxidase and glutathione reductase enzymes were evaluated with the Western blot method. Resveratrol increased the reduced glutathione contents significantly (p < 0.05). Resveratrol increased protein expression of the glutamate cysteine ligase, glutathione peroxidase-1 and glutathione reductase enzymes (p < 0.05). All data supported each other and suggested that resveratrol had a protective effect against human coronary ar...
Journal of the Turkish German Gynecological Association, 2013
To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by ... more To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemistry in a rat uterine horn model with full thickness injury. Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically. Adhesion scores were 2.00±0.81, 2.14±0.69 0.71±0.75, and 0.85±0.69 for extent and 2.28±0.48, 2.14±0.69, 0.85±0.69, and 1.14±0.69 for severity in Groups 1, 2, 3 and 4, respectively. Adhesion extent and severity were significantly less for groups treated by lipoic acid but no difference was observed between long and short administration. Both Vitronectin and u-PAR staining were significantly increased in treatment groups when compared to the control group. Lipoic acid was found to be effective in reducing postoperative adhesion formation in a rat model.
Journal of Pediatric Endocrinology and Metabolism, 2014
Nonalcoholic fatty liver disease (NAFLD) is the accumulation of excess fat in the liver in the ab... more Nonalcoholic fatty liver disease (NAFLD) is the accumulation of excess fat in the liver in the absence of alcohol consumption, which is commonly associated with obesity and increased risk of atherosclerosis as well as insulin resistance. Adropin is a recently identified protein encoded by the gene related with energy homeostasis, which is expressed in the liver and the brain and has a role in preventing insulin resistance and obesity. The aim of this study was to investigate the serum adropin and leptin levels in obese adolescents and compare the patients with, and without, NAFLD and with healthy controls. Sixty-four obese adolescents (30 with NAFLD, 34 without NAFLD) and 36 healthy controls were enrolled in the study. Serum adropin and leptin levels were evaluated by sandwich enzyme-linked immunosorbent assay. Serum adropin levels were significantly lower in obese children than healthy controls (3.2±1.0 and 9.2±1.2 ng/mL, respectively, p=0.001). Serum leptin levels were significantly higher in patients than in controls (12.4±1.1 and 4.1±3.1 pg/mL, respectively; p=0.000). Serum adropin levels of patients with NAFLD were significantly lower than in patients without NAFLD (2.9±0.5 and 3.5±1.2 ng/mL, respectively; p=0.023) and healthy controls (p=0.000). Logistic regression analysis showed that a decrease in adropin levels was the only independent factor for fatty liver disease in obese adolescents (odds ratio: 3.07, 95% confidence interval 1.14-8.2, p=0.026). Leptin, relative weight and HOMA-IR of the patients were not independent risk factors for NAFLD. In this study, serum adropin levels were significantly lower in obese adolescents with fatty liver disease compared to patients without fatty liver disease and healthy controls. Lower adropin level was an independent risk factor for NAFLD in obese adolescents in logistic regression analysis. Assessment of serum adropin concentrations may provide a reliable indicator of fatty liver disease in obese adolescents.
The objective of this study was to investigate the effect of resveratrol on the healing process a... more The objective of this study was to investigate the effect of resveratrol on the healing process after midline laparotomy in rats. The study was performed on adult female Wistar-Albino rats. The study group was orally administered 0.5 mg/kg resveratrol once a day for 7 days before the operation until 12 h before surgery and then the treatment was maintained throughout the study. Each rat was anesthetized, and a 4-cm midline laparotomy was performed. Ten animals in each group were sacrificed on postoperative days 7, and 14. A tensile strength analysis was performed, hydroxyproline levels were measured, and the abdominal incision wounds were examined histologically. Resveratrol administration significantly increased the tensile strength of the abdominal fascia, and increased the hydroxyproline levels on postoperative day 14. The acute inflammation scores, collagen deposition scores and the neovascularization scores on postoperative days 7 and 14 were found to be significantly higher in the resveratrol treatment group compared to the control group. The amount of granulation tissue and the fibroblast maturation scores were found to be significantly higher only on postoperative day 14 in the treatment group compared to the control group. Our findings show that resveratrol may have a beneficial effect on incisional wound healing.
Journal of Pediatric Endocrinology and Metabolism, 2000
Nonalcoholic fatty liver disease (NAFLD) is associated with inflammation and increased risk of at... more Nonalcoholic fatty liver disease (NAFLD) is associated with inflammation and increased risk of atherosclerosis. Neopterin is regarded as a biochemical marker of cell-mediated immunity, which is secreted by monocytes and macrophages, mainly in response to interferon-gamma. The aim of the present study was to investigate the serum neopterin levels in obese adolescents and compare the neopterin levels in patients with and without NAFLD and also with healthy controls. The second aim of the study was to research the possible relationship between neopterin levels and adipokines (leptin, adiponectin, resistin, and ghrelin). Ninety-three obese adolescents (39 with NAFLD, 54 without NAFLD) and 55 healthy controls were enrolled in the study. Serum levels of neopterin and adipokines were measured with high-performance liquid chromatography and sandwich enzyme-linked immunosorbent assay, respectively. Serum neopterin levels were significantly higher in patients with NAFLD (3.20 ± 0.09 nmol/L) than in their healthy peers (2.91 ± 0.08 nmol/L) (p=0.020). Neopterin levels were positively correlated with leptin levels in obese patients (r=0.380, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) and in the group comprising all individuals (r=0.206, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). There was no correlation between neopterin concentrations and relative weight, alanin aminotransferase, adiponectin, resistin, and ghrelin levels. The serum neopterin levels were significantly higher in obese adolescents with fatty liver disease compared to controls, and this may be related to increased cell-mediated immunity in fatty liver disease.
Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immun... more Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.
Evidence-Based Complementary and Alternative Medicine, 2013
This study compared the effects of transcutaneous electrical nerve stimulation (TENS), saline sol... more This study compared the effects of transcutaneous electrical nerve stimulation (TENS), saline solution (SS), povidone-iodine (PI), and lavender oil (Lavandula angustifolia) through expression of growth factors in a rat model of wound healing. Six experimental groups were established, each containing 8 rats: a healthy group with no incision wounds, an incision-control group, an incision and TENS group, an incision and SS group, an incision and PI group, and an incision and lavender oil group. Experiments continued for 5 days, after which the skin in the excision area was removed. Tissue concentrations of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-A were measured using enzyme-linked immunosorbent assay (ELISA). Tissue expressions of EGF, PDGF-A, and fibroblast growth factor (FGF)-2 were determined using immunohistochemistry. Wound closure progressed more rapidly in the TENS and lavender oil groups than in the control and other study groups. In particular, PDGF-A expressions in the dermis and EGF expression in the epidermis were significantly intense in the TENS group ( < 0.05). In addition, ELISA levels of growth factors such as PDGF-A and EGF were significantly higher in TENS group compared to the control group ( < 0.05). These immunohistochemical and ELISA results suggest that TENS may improve wound healing through increasing growth factors in the dermis and epidermis more than other topical applications.
Türk Biyokimya Dergisi [Turkish Journal of …, 2008
Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile kar... more Süperoksit ve hidrojen peroksit gibi reaktif oksijen ürünlerinin endojen üretiminde artış ile karakterize olan oksidatif stres, endotel tabakasının işlev bozukluğunun temel nedenidir. Oksidatif stres kardiyovasküler hastalıklar, kanser, nörolojik hastalıklar, diyabet, yaşlanma ve diğer pek çok hastalığın patogenezinde önemli bir rol oynamaktadır. Oksidatif stres ile ilgili savunma mekanizmaları; bu mekanizmaların engellenmesi, onarımı, fiziksel savunma ve antioksidan savunmayı kapsamaktadır. Hipoksi-reoksijenasyon (ya da iskemi-reperfüzyon) hasarı kalpte hem koroner damarları (damar düz kası ve endotel), hem de kalp kası hücrelerini (kardiyak miyositleri) etkilemektedir. Koroner damarlardaki hasar, damar tonusunu değiştirebilir ve reperfüzyon sırasında koroner kan akışını etkileyebilir. Kas hücrelerindeki iskemi-reperfüzyon hasarına ek olarak, koroner akışın azalması miyokardiyal disfonksiyonu arttırmakta, bu da ölüm ve hastalıkla sonuçlanmaktadır. Enzimatik antioksidan savunma süperoksit dismutaz, glutatyon peroksidaz ve katalazı kapsamaktadır. Enzimatik olmayan antioksidanlar askorbik asit, α-tokoferol, glutatyon, karotenoidler, flavonoidler, stilbenler ve diğerleridir. Normal koşullar altında bu antioksidanların hücre içi düzeyi ve etkinlikleri arasında denge bulunmaktadır. Bu denge, organizmanın canlılığı ve sağlığı açısından gereklidir. Resveratrol, stilbenlerin alt grubu olup, üzüm ve kırmızı şarapta bulunan polifenolik bir bileşiktir. Anti-inflamatuvar, antioksidan, anti-apoptotik, sitoprotektif, anti-kanser ve kardioprotektif etkileri bulunmaktadır. Fransa'da kırmızı şarap tüketimi ile kardiovasküler hastalık sıklığı arasında ters orantı saptanmıştır (Fransız paradoksu). Kardiyak hasarın akut ve kronik modellerinde, resveratrol miyokardiyal iskemireperfüzyon hasarının şiddetini azaltmaktadır. Kardiyovasküler hastalıkların patofizyolojisinde rol oynayan oksidatif hasara karşı koruyucu olduğu, yapılan çok sayıda çalışma ile gösterilmiştir.
International Journal for Vitamin and Nutrition Research, 2012
The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular u... more The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Among the human matrix metalloproteinases (MMPs), MMP-2 and -9, known as gelatinases, are the key enzymes for degrading type IV collagen, which is the major component of the basal membrane that surrounds the cerebral blood vessel. In the present study, we investigated the effects of resveratrol on cytotoxicity, reactive oxygen species (ROS), and gelatinases (MMP-2 and -9) in human cerebral microvascular endothelial cells exposed to 6 hours of oxygen-glucose deprivation and a subsequent 24 hours of reoxygenation with glucose (OGD/R), to mimic ischemia/reperfusion in vivo. Lactate dehydrogenase increased significantly, in comparison to that in the normoxia group. ROS was markedly increased in the OGD/R group, compared to normoxia. Correspondingly, ROS was significantly reduced with 50 μM of resveratrol. The proMMP-2 activity in the OGD/R group showed a statistically significant increase from the control cells. Resveratrol preconditioning decreased significantly the proMMP-2 in the cells exposed to OGD/R in comparison to that in the OGD/R group. Our results indicate that resveratrol regulates MMP-2 activity induced by OGD/R via its antioxidant effect, implying a possible mechanism related to the neuroprotective effect of resveratrol.
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