The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of ... more The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes of Dipterygium glaucum and surrounding bulk soil microbiomes, and to detect differential microbial responses due to watering. Preliminary results reflect the reliability of the experiment and the rationality of grouping microbiomes. Based on the abundance of non-redundant genes, bacterial genomes showed the highest level, followed by Archaeal and Eukaryotic genomes, then, the least abundant viruses. Overall results indicate that most members of bacteria have a higher abundance/relative abundance (AB/RA) pattern in the rhizosphere towards plant growth promotion, while members of eukaryota have a higher pattern in bulk soil, most likely acting as pathogens. The results also indicate the contribution of mycorrhiza (genus Rhizophagus) in mediating complex mutualistic associations between soil microbes (either beneficial or harmful) and plant roots. Some of these s...
The present study aimed to predict differential enrichment of pathways and compounds in the rhizo... more The present study aimed to predict differential enrichment of pathways and compounds in the rhizosphere microbiomes of the two wild plants (Abutilon fruticosum and Nitrosalsola vermiculata) and to predict functional shifts in microbiomes due to water. Amplicon sequencing of 16S rRNA region V3-V4 was done and gene-based microbial compositions were enrolled in PICRUSt to predict enriched pathways and compounds. The results indicated that "ABC transporters" and "Quorum sensing" pathways are among the highest enriched pathways in rhizosphere microbiomes of the two wild plants compared with those of the bulk soil microbiomes. The highest enriched compounds in soil microbiomes of the two wild plants included five proteins and three enzymes participating in one or more KEGG pathways. Six of these eight compounds showed higher predicted enrichment in rhizosphere soil microbiomes, while only one, namely phosphate transport system substrate-binding protein, showed higher enrichment in the surrounding bulk soil microbiomes. In terms of differentially enriched compounds due to watering, only the dual-specific aspartyl-tRNA (Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A showed higher enrichment in rhizosphere soil of the two wild plants after 24 h of watering. Two of the highly enriched compounds namely branched-chain amino acid transport system ATP-binding protein and branched-chain amino acid transport system substrate-binding protein, are encoded by genes stimulated by the plant's GABA that participates in conferring biotic and abiotic stresses in plants and improves the plant's growth performance. The 3-Oxoacyl-[ACP] reductase, a member of the short-chain alcohol dehydrogenase/ reductase (SDR) superfamily, participates in fatty acids elongation cycles and contributes to plant-microbe symbiotic relationships, while enoyl-CoA hydratase has a reverse action as it participates in "Fatty acid degradation" pathway. The methyl-accepting chemotaxis protein is an environmental signal that sense "Bacterial chemotaxis" pathway to help establishing symbiosis with plant roots by recruiting/colonizing of microbial partners (symbionts) to plant rhizosphere. This information justifies the high enrichment of compounds in plant rhizosphere. The dual-specific aspartyl-tRNA (Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A contributes to the plant ability to respond to watering as it participates in attaching the correct amino acid during translation to its This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study was carried out to determine the consequence of foliar application of agmatine (0 and ... more This study was carried out to determine the consequence of foliar application of agmatine (0 and 0.5 mM), on growth, physiological and biochemical traits, and yield of turnip (Brassica rapa L.) plants grown under water stress or salt stress conditions. The effect of three irrigation regimes (100%, 80% and 40% of field capacity) and three salt concentrations (0, 100 and 200 mM NaCl) on turnip plants grown in pots under greenhouse conditions were studied. Water deficit developed at 40% Field capacity (FC) and salinity stress, especially at 200 mM, resulted in significant decreases in all growth parameters when compared to control plants (100% FC) including root length and diameter as well as shoot weights per plant. Water stress and high salt stress negatively affected most physiological and biochemical characteristics such as total chlorophyll, photosynthetic rate, stomatal conductance, and transpiration rate. Water use efficiency (WUE) increased under 80% FC or 100 mM NaCl. Antioxid...
The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of ... more The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes of Dipterygium glaucum and surrounding bulk soil microbiomes, and to detect differential microbial responses due to watering. Preliminary results reflect the reliability of the experiment and the rationality of grouping microbiomes. Based on the abundance of non-redundant genes, bacterial genomes showed the highest level, followed by Archaeal and Eukaryotic genomes, then, the least abundant viruses. Overall results indicate that most members of bacteria have a higher abundance/relative abundance (AB/RA) pattern in the rhizosphere towards plant growth promotion, while members of eukaryota have a higher pattern in bulk soil, most likely acting as pathogens. The results also indicate the contribution of mycorrhiza (genus Rhizophagus) in mediating complex mutualistic associations between soil microbes (either beneficial or harmful) and plant roots. Some of these s...
The present study aimed to predict differential enrichment of pathways and compounds in the rhizo... more The present study aimed to predict differential enrichment of pathways and compounds in the rhizosphere microbiomes of the two wild plants (Abutilon fruticosum and Nitrosalsola vermiculata) and to predict functional shifts in microbiomes due to water. Amplicon sequencing of 16S rRNA region V3-V4 was done and gene-based microbial compositions were enrolled in PICRUSt to predict enriched pathways and compounds. The results indicated that "ABC transporters" and "Quorum sensing" pathways are among the highest enriched pathways in rhizosphere microbiomes of the two wild plants compared with those of the bulk soil microbiomes. The highest enriched compounds in soil microbiomes of the two wild plants included five proteins and three enzymes participating in one or more KEGG pathways. Six of these eight compounds showed higher predicted enrichment in rhizosphere soil microbiomes, while only one, namely phosphate transport system substrate-binding protein, showed higher enrichment in the surrounding bulk soil microbiomes. In terms of differentially enriched compounds due to watering, only the dual-specific aspartyl-tRNA (Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A showed higher enrichment in rhizosphere soil of the two wild plants after 24 h of watering. Two of the highly enriched compounds namely branched-chain amino acid transport system ATP-binding protein and branched-chain amino acid transport system substrate-binding protein, are encoded by genes stimulated by the plant's GABA that participates in conferring biotic and abiotic stresses in plants and improves the plant's growth performance. The 3-Oxoacyl-[ACP] reductase, a member of the short-chain alcohol dehydrogenase/ reductase (SDR) superfamily, participates in fatty acids elongation cycles and contributes to plant-microbe symbiotic relationships, while enoyl-CoA hydratase has a reverse action as it participates in "Fatty acid degradation" pathway. The methyl-accepting chemotaxis protein is an environmental signal that sense "Bacterial chemotaxis" pathway to help establishing symbiosis with plant roots by recruiting/colonizing of microbial partners (symbionts) to plant rhizosphere. This information justifies the high enrichment of compounds in plant rhizosphere. The dual-specific aspartyl-tRNA (Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A contributes to the plant ability to respond to watering as it participates in attaching the correct amino acid during translation to its This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study was carried out to determine the consequence of foliar application of agmatine (0 and ... more This study was carried out to determine the consequence of foliar application of agmatine (0 and 0.5 mM), on growth, physiological and biochemical traits, and yield of turnip (Brassica rapa L.) plants grown under water stress or salt stress conditions. The effect of three irrigation regimes (100%, 80% and 40% of field capacity) and three salt concentrations (0, 100 and 200 mM NaCl) on turnip plants grown in pots under greenhouse conditions were studied. Water deficit developed at 40% Field capacity (FC) and salinity stress, especially at 200 mM, resulted in significant decreases in all growth parameters when compared to control plants (100% FC) including root length and diameter as well as shoot weights per plant. Water stress and high salt stress negatively affected most physiological and biochemical characteristics such as total chlorophyll, photosynthetic rate, stomatal conductance, and transpiration rate. Water use efficiency (WUE) increased under 80% FC or 100 mM NaCl. Antioxid...
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