International Journal of Current Microbiology and Applied Sciences, 2020
Escherichia coli are commensal organism in the gastrointestinal tract of warm-blooded animals and... more Escherichia coli are commensal organism in the gastrointestinal tract of warm-blooded animals and it has been extensively used to monitor antimicrobial resistance in food animals including poultry. During the past few decades, drug resistance in E.coli has increased dramatically worldwide. Diverse class of antimicrobials is used to raise poultry International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 3 (2020) Journal homepage: http://www.ijcmas.com
Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (inv... more Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes, Veterinary World, 10(7): 814-817.
Milk is considered as complete food and an important part of human diet throughout the world incl... more Milk is considered as complete food and an important part of human diet throughout the world including India. Bacterial contamination of milk such as due to unhygienic condition and poor udder health can cause infections, especially in infants and elders or in immunocompromised persons. Possession of antimicrobial resistance genes by commensal bacteria present in milk makes the issue more serious. The study was aimed to isolate and characterize extended-spectrum beta-lactamase (ESBL)-producing from milk samples collected from different parts of West Bengal, India, to assess the potential risk associated with the food. Around 182 milk samples were collected from apparently healthy cows reared by organized dairy farms in West Bengal. was isolated from collected samples as per standard methods followed by serotyping. The detection of ESBL-producing was done both phenotypically and genotypically by detecting the presence of gene. Antibiogram of the ESBL-positive isolates was done using ...
Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dair... more Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dairy farmers, of which Pseudomonas aeruginosa is one of the causative agents. The study was aimed at a screening of suspected milk samples from different cattle farms of West Bengal for detection and confirmation of P. aeruginosa strains followed by their characterization. Materials and Methods: Around 422 milk samples were screened from different dairy farms primarily by on-spot bromothymol blue (BTB) test and then in the lab by somatic cell counts (SCC) to finally consider 352 samples for detection of P. aeruginosa. Selective isolation and confirmation of the isolates were done using selective media, viz., cetrimide and Pseudomonas agar followed by confirmation by fluorescent technique. Molecular characterization of the strains was done by polymerase chain reaction for the presence of toxA (enterotoxin A, 352 bp) and exoS (exoenzyme S, 504 bp) genes. Results: Approximately, 371 (87.9%) samples were positive in on-spot BTB test among which 352 (94.8%) samples revealed high SCC values (more than 3 lakh cells/ml) showing infection when screened. Among these, 23 (6.5%) samples yielded typical Pseudomonas sp. isolates out of which only 19 (5.4%) isolates were confirmed to be P. aeruginosa which showed characteristic blue-green fluorescence due to the presence of pigment pyoverdin under ultraviolet light. Out of these 19 isolates, 11 isolates were positive for toxA, 6 isolates for exoS, and 2 for both these pathogenic genes. Conclusion: Approximately, 5.4% cases of bovine subclinical mastitis infections in South Bengal were associated with P. aeruginosa which possess pathogenic genes such as toxA (63.2%) and exoS (36.8%).
International Journal of Current Microbiology and Applied Sciences, 2020
Escherichia coli are commensal organism in the gastrointestinal tract of warm-blooded animals and... more Escherichia coli are commensal organism in the gastrointestinal tract of warm-blooded animals and it has been extensively used to monitor antimicrobial resistance in food animals including poultry. During the past few decades, drug resistance in E.coli has increased dramatically worldwide. Diverse class of antimicrobials is used to raise poultry International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 3 (2020) Journal homepage: http://www.ijcmas.com
Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (inv... more Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes, Veterinary World, 10(7): 814-817.
Milk is considered as complete food and an important part of human diet throughout the world incl... more Milk is considered as complete food and an important part of human diet throughout the world including India. Bacterial contamination of milk such as due to unhygienic condition and poor udder health can cause infections, especially in infants and elders or in immunocompromised persons. Possession of antimicrobial resistance genes by commensal bacteria present in milk makes the issue more serious. The study was aimed to isolate and characterize extended-spectrum beta-lactamase (ESBL)-producing from milk samples collected from different parts of West Bengal, India, to assess the potential risk associated with the food. Around 182 milk samples were collected from apparently healthy cows reared by organized dairy farms in West Bengal. was isolated from collected samples as per standard methods followed by serotyping. The detection of ESBL-producing was done both phenotypically and genotypically by detecting the presence of gene. Antibiogram of the ESBL-positive isolates was done using ...
Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dair... more Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dairy farmers, of which Pseudomonas aeruginosa is one of the causative agents. The study was aimed at a screening of suspected milk samples from different cattle farms of West Bengal for detection and confirmation of P. aeruginosa strains followed by their characterization. Materials and Methods: Around 422 milk samples were screened from different dairy farms primarily by on-spot bromothymol blue (BTB) test and then in the lab by somatic cell counts (SCC) to finally consider 352 samples for detection of P. aeruginosa. Selective isolation and confirmation of the isolates were done using selective media, viz., cetrimide and Pseudomonas agar followed by confirmation by fluorescent technique. Molecular characterization of the strains was done by polymerase chain reaction for the presence of toxA (enterotoxin A, 352 bp) and exoS (exoenzyme S, 504 bp) genes. Results: Approximately, 371 (87.9%) samples were positive in on-spot BTB test among which 352 (94.8%) samples revealed high SCC values (more than 3 lakh cells/ml) showing infection when screened. Among these, 23 (6.5%) samples yielded typical Pseudomonas sp. isolates out of which only 19 (5.4%) isolates were confirmed to be P. aeruginosa which showed characteristic blue-green fluorescence due to the presence of pigment pyoverdin under ultraviolet light. Out of these 19 isolates, 11 isolates were positive for toxA, 6 isolates for exoS, and 2 for both these pathogenic genes. Conclusion: Approximately, 5.4% cases of bovine subclinical mastitis infections in South Bengal were associated with P. aeruginosa which possess pathogenic genes such as toxA (63.2%) and exoS (36.8%).
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